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Reviewed, UniProtKB/Swiss-Prot P05466 (AROA_ARATH)

Last modified November 3, 2009. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
Gene names
Ordered Locus Names: At2g45300
ORF Names: F4L23.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.

Subcellular location

Plastidchloroplast.

Miscellaneous

This enzyme is the target of the potent, broad-spectrum herbicide, glyphosate [n-(phosphonomethyl)glycine]. Overproduction of EPSP leads to glyphosate tolerance.

Sequence similarities

Belongs to the EPSP synthase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7676Chloroplast Potential
Chain77 – 5204443-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic
PRO_0000002287

Experimental info

Sequence conflict3331S → C Ref.1
Sequence conflict3331S → C Ref.2
Sequence conflict5001P → S Ref.1
Sequence conflict5001P → S Ref.2

Sequences

Sequence LengthMass (Da)Tools
P05466-1 [UniParc].

Last modified February 5, 2008. Version 3.
Checksum: 79418663170D0809

FASTA52055,734
        10         20         30         40         50         60 
MAQVSRICNG VQNPSLISNL SKSSQRKSPL SVSLKTQQHP RAYPISSSWG LKKSGMTLIG 

        70         80         90        100        110        120 
SELRPLKVMS SVSTAEKASE IVLQPIREIS GLIKLPGSKS LSNRILLLAA LSEGTTVVDN 

       130        140        150        160        170        180 
LLNSDDINYM LDALKRLGLN VETDSENNRA VVEGCGGIFP ASIDSKSDIE LYLGNAGTAM 

       190        200        210        220        230        240 
RPLTAAVTAA GGNASYVLDG VPRMRERPIG DLVVGLKQLG ADVECTLGTN CPPVRVNANG 

       250        260        270        280        290        300 
GLPGGKVKLS GSISSQYLTA LLMSAPLALG DVEIEIVDKL ISVPYVEMTL KLMERFGVSV 

       310        320        330        340        350        360 
EHSDSWDRFF VKGGQKYKSP GNAYVEGDAS SASYFLAGAA ITGETVTVEG CGTTSLQGDV 

       370        380        390        400        410        420 
KFAEVLEKMG CKVSWTENSV TVTGPPRDAF GMRHLRAIDV NMNKMPDVAM TLAVVALFAD 

       430        440        450        460        470        480 
GPTTIRDVAS WRVKETERMI AICTELRKLG ATVEEGSDYC VITPPKKVKT AEIDTYDDHR 

       490        500        510        520 
MAMAFSLAAC ADVPITINDP GCTRKTFPDY FQVLERITKH 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase: sequence analysis and manipulation to obtain glyphosate-tolerant plants."
Klee H.J., Muskopf Y.M., Gasser C.S.
Mol. Gen. Genet. 210:437-442(1987) [PubMed: 3481024] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]Gasser C.S.
Submitted (OCT-1988) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

X06613 Genomic DNA. Translation: CAA29828.1.
AC002387 Genomic DNA. Translation: AAB82633.1.
BT022026 mRNA. Translation: AAY25438.1.
AK227120 mRNA. Translation: BAE99170.1.
IPIIPI00538860.
PIRH84888.
XUMUVS. S01061.
RefSeqNP_182055.1.
UniGeneAt.43670

3D structure databases

HSSPHSSP built from PDB template 1G6S based on UniProtKB P07638.
ModBaseSearch...

Protein-protein interaction databases

STRINGP05466.

Proteomic databases

PRIDEP05466.

Genome annotation databases

GeneID819138.
GenomeReviewsGene locus AT2G45300 in contig CT485783_GR.
KEGGath:AT2G45300.

Organism-specific databases

TAIRAt2g45300.

Phylogenomic databases

OMADWRAASI.

Enzyme and pathway databases

BRENDA2.5.1.19. 302.

Gene expression databases

ArrayExpressP05466.
GenevestigatorP05466.
GermOnlineAT2G45300. Arabidopsis thaliana.

Family and domain databases

InterProIPR001986. EPSP_synthase_core.
IPR006264. EPSP_synthase_subgroup.
[Graphical view]
Gene3DG3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PfamPF00275. EPSP_synthase. 1 hit.
[Graphical view]
ProDomPD001867. EPSP_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01356. aroA. 1 hit.
PROSITEPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAROA_ARATH
AccessionPrimary (citable) accession number: P05466
Secondary accession number(s): O22142, Q501E1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 5, 2008
Last modified: November 3, 2009
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents