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Protein

3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic

Gene

At2g45300

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.By similarity

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.

Pathwayi: chorismate biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Phospho-2-dehydro-3-deoxyheptonate aldolase, Phospho-2-dehydro-3-deoxyheptonate aldolase (DHS2), Phospho-2-dehydro-3-deoxyheptonate aldolase 2, chloroplastic (DHS2), Phospho-2-dehydro-3-deoxyheptonate aldolase (At4g33510), Phospho-2-dehydro-3-deoxyheptonate aldolase (At4g33510), Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic (DHS1), Phospho-2-dehydro-3-deoxyheptonate aldolase (F12K8.24)
  2. 3-dehydroquinate synthase, chloroplastic (DHQS)
  3. Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (EMB3004)
  4. Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (EMB3004)
  5. Shikimate kinase 1, chloroplastic (SK1), Shikimate kinase 2, chloroplastic (SK2)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (F27K7.11), 3-phosphoshikimate 1-carboxyvinyltransferase (At1g48860), 3-phosphoshikimate 1-carboxyvinyltransferase, 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic (At2g45300)
  7. Chorismate synthase (At1g48850), Chorismate synthase (EMB1144), Chorismate synthase (EMB1144), Chorismate synthase, chloroplastic (EMB1144)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041Shikimate-3-phosphateBy similarity
Binding sitei207 – 2071PhosphoenolpyruvateBy similarity
Binding sitei282 – 2821Shikimate-3-phosphateBy similarity
Active sitei407 – 4071Proton acceptorBy similarity
Binding sitei434 – 4341Shikimate-3-phosphateBy similarity
Active sitei435 – 4351Proton donorBy similarity
Binding sitei438 – 4381PhosphoenolpyruvateBy similarity
Binding sitei480 – 4801PhosphoenolpyruvateBy similarity
Binding sitei505 – 5051PhosphoenolpyruvateBy similarity

GO - Molecular functioni

  • 3-phosphoshikimate 1-carboxyvinyltransferase activity Source: TAIR

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: UniProtKB-KW
  • chorismate biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciARA:AT2G45300-MONOMER.
UniPathwayiUPA00053; UER00089.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic (EC:2.5.1.19)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name:
EPSP synthase
Gene namesi
Ordered Locus Names:At2g45300
ORF Names:F4L23.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G45300.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7676ChloroplastSequence analysisAdd
BLAST
Chaini77 – 5204443-phosphoshikimate 1-carboxyvinyltransferase, chloroplasticPRO_0000002287Add
BLAST

Proteomic databases

PaxDbiP05466.
PRIDEiP05466.

PTM databases

iPTMnetiP05466.

Expressioni

Gene expression databases

GenevisibleiP05466. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G45300.1.

Structurei

3D structure databases

ProteinModelPortaliP05466.
SMRiP05466. Positions 81-519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 1002Shikimate-3-phosphate bindingBy similarity
Regioni175 – 1784PhosphoenolpyruvateBy similarity
Regioni254 – 2563Shikimate-3-phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the EPSP synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IRXE. Eukaryota.
COG0128. LUCA.
HOGENOMiHOG000247372.
InParanoidiP05466.
KOiK00800.
OMAiMAFADCG.
PhylomeDBiP05466.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQVSRICNG VQNPSLISNL SKSSQRKSPL SVSLKTQQHP RAYPISSSWG
60 70 80 90 100
LKKSGMTLIG SELRPLKVMS SVSTAEKASE IVLQPIREIS GLIKLPGSKS
110 120 130 140 150
LSNRILLLAA LSEGTTVVDN LLNSDDINYM LDALKRLGLN VETDSENNRA
160 170 180 190 200
VVEGCGGIFP ASIDSKSDIE LYLGNAGTAM RPLTAAVTAA GGNASYVLDG
210 220 230 240 250
VPRMRERPIG DLVVGLKQLG ADVECTLGTN CPPVRVNANG GLPGGKVKLS
260 270 280 290 300
GSISSQYLTA LLMSAPLALG DVEIEIVDKL ISVPYVEMTL KLMERFGVSV
310 320 330 340 350
EHSDSWDRFF VKGGQKYKSP GNAYVEGDAS SASYFLAGAA ITGETVTVEG
360 370 380 390 400
CGTTSLQGDV KFAEVLEKMG CKVSWTENSV TVTGPPRDAF GMRHLRAIDV
410 420 430 440 450
NMNKMPDVAM TLAVVALFAD GPTTIRDVAS WRVKETERMI AICTELRKLG
460 470 480 490 500
ATVEEGSDYC VITPPKKVKT AEIDTYDDHR MAMAFSLAAC ADVPITINDP
510 520
GCTRKTFPDY FQVLERITKH
Length:520
Mass (Da):55,734
Last modified:February 5, 2008 - v3
Checksum:i79418663170D0809
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti333 – 3331S → C in CAA29828 (PubMed:3481024).Curated
Sequence conflicti500 – 5001P → S in CAA29828 (PubMed:3481024).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06613 Genomic DNA. Translation: CAA29828.1.
AC002387 Genomic DNA. Translation: AAB82633.1.
CP002685 Genomic DNA. Translation: AEC10536.1.
BT022026 mRNA. Translation: AAY25438.1.
AK227120 mRNA. Translation: BAE99170.1.
PIRiH84888.
S01061. XUMUVS.
RefSeqiNP_182055.1. NM_130093.2.
UniGeneiAt.43670.

Genome annotation databases

EnsemblPlantsiAT2G45300.1; AT2G45300.1; AT2G45300.
GeneIDi819138.
GrameneiAT2G45300.1; AT2G45300.1; AT2G45300.
KEGGiath:AT2G45300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06613 Genomic DNA. Translation: CAA29828.1.
AC002387 Genomic DNA. Translation: AAB82633.1.
CP002685 Genomic DNA. Translation: AEC10536.1.
BT022026 mRNA. Translation: AAY25438.1.
AK227120 mRNA. Translation: BAE99170.1.
PIRiH84888.
S01061. XUMUVS.
RefSeqiNP_182055.1. NM_130093.2.
UniGeneiAt.43670.

3D structure databases

ProteinModelPortaliP05466.
SMRiP05466. Positions 81-519.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G45300.1.

PTM databases

iPTMnetiP05466.

Proteomic databases

PaxDbiP05466.
PRIDEiP05466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G45300.1; AT2G45300.1; AT2G45300.
GeneIDi819138.
GrameneiAT2G45300.1; AT2G45300.1; AT2G45300.
KEGGiath:AT2G45300.

Organism-specific databases

TAIRiAT2G45300.

Phylogenomic databases

eggNOGiENOG410IRXE. Eukaryota.
COG0128. LUCA.
HOGENOMiHOG000247372.
InParanoidiP05466.
KOiK00800.
OMAiMAFADCG.
PhylomeDBiP05466.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.
BioCyciARA:AT2G45300-MONOMER.

Miscellaneous databases

PROiP05466.

Gene expression databases

GenevisibleiP05466. AT.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase: sequence analysis and manipulation to obtain glyphosate-tolerant plants."
    Klee H.J., Muskopf Y.M., Gasser C.S.
    Mol. Gen. Genet. 210:437-442(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. Gasser C.S.
    Submitted (OCT-1988) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiAROA_ARATH
AccessioniPrimary (citable) accession number: P05466
Secondary accession number(s): O22142, Q501E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 5, 2008
Last modified: February 17, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

This enzyme is the target of the potent, broad-spectrum herbicide, glyphosate [n-(phosphonomethyl)glycine]. Overproduction of EPSP leads to glyphosate tolerance.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.