Reviewed, UniProtKB/Swiss-Prot P05466 (AROA_ARATH)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase

Gene names

Ordered Locus Names:	At2g45300
ORF Names:	F4L23.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.
Subcellular location	Plastid › chloroplast.
Miscellaneous	This enzyme is the target of the potent, broad-spectrum herbicide, glyphosate [n-(phosphonomethyl)glycine]. Overproduction of EPSP leads to glyphosate tolerance.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW chorismate biosynthetic process Ref.1 Inferred from mutant phenotype. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Ref.1 Inferred from mutant phenotype. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 76	76	Chloroplast Potential
	Chain	77 – 520	444	3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic		PRO_0000002287
Experimental info
	Sequence conflict	333	1	S → C Ref.1
	Sequence conflict	333	1	S → C Ref.2
	Sequence conflict	500	1	P → S Ref.1
	Sequence conflict	500	1	P → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05466-1 [UniParc]. Last modified February 5, 2008. Version 3. Checksum: 79418663170D0809	FASTA	520	55,734
	10 20 30 40 50 60 MAQVSRICNG VQNPSLISNL SKSSQRKSPL SVSLKTQQHP RAYPISSSWG LKKSGMTLIG 70 80 90 100 110 120 SELRPLKVMS SVSTAEKASE IVLQPIREIS GLIKLPGSKS LSNRILLLAA LSEGTTVVDN 130 140 150 160 170 180 LLNSDDINYM LDALKRLGLN VETDSENNRA VVEGCGGIFP ASIDSKSDIE LYLGNAGTAM 190 200 210 220 230 240 RPLTAAVTAA GGNASYVLDG VPRMRERPIG DLVVGLKQLG ADVECTLGTN CPPVRVNANG 250 260 270 280 290 300 GLPGGKVKLS GSISSQYLTA LLMSAPLALG DVEIEIVDKL ISVPYVEMTL KLMERFGVSV 310 320 330 340 350 360 EHSDSWDRFF VKGGQKYKSP GNAYVEGDAS SASYFLAGAA ITGETVTVEG CGTTSLQGDV 370 380 390 400 410 420 KFAEVLEKMG CKVSWTENSV TVTGPPRDAF GMRHLRAIDV NMNKMPDVAM TLAVVALFAD 430 440 450 460 470 480 GPTTIRDVAS WRVKETERMI AICTELRKLG ATVEEGSDYC VITPPKKVKT AEIDTYDDHR 490 500 510 520 MAMAFSLAAC ADVPITINDP GCTRKTFPDY FQVLERITKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase: sequence analysis and manipulation to obtain glyphosate-tolerant plants." Klee H.J., Muskopf Y.M., Gasser C.S. Mol. Gen. Genet. 210:437-442(1987) [PubMed: 3481024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	Gasser C.S. Submitted (OCT-1988) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X06613 Genomic DNA. Translation: CAA29828.1. AC002387 Genomic DNA. Translation: AAB82633.1. BT022026 mRNA. Translation: AAY25438.1. AK227120 mRNA. Translation: BAE99170.1.
IPI	IPI00538860.
PIR	H84888. XUMUVS. S01061.
RefSeq	NP_182055.1.
UniGene	At.43670
3D structure databases
HSSP	HSSP built from PDB template 1G6S based on UniProtKB P07638.
ModBase	Search...
Protein-protein interaction databases
STRING	P05466.
Proteomic databases
PRIDE	P05466.
Genome annotation databases
GeneID	819138.
GenomeReviews	Gene locus AT2G45300 in contig CT485783_GR.
KEGG	ath:AT2G45300.
Organism-specific databases
TAIR	At2g45300.
Phylogenomic databases
OMA	DWRAASI.
Enzyme and pathway databases
BRENDA	2.5.1.19. 302.
Gene expression databases
ArrayExpress	P05466.
Genevestigator	P05466.
GermOnline	AT2G45300. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_ARATH

Accession

Primary (citable) accession number: P05466
Secondary accession number(s): O22142, Q501E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	February 5, 2008
Last modified:	November 3, 2009
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)