P05465 (DHGA_ACICA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Quinoprotein glucose dehydrogenase A
EC=1.1.99.35

Alternative name(s):
GDH-A
Glucose dehydrogenase A [pyrroloquinoline-quinone]
Quinoprotein glucose DH

Gene names

Name:

gdhA

Organism

Acinetobacter calcoaceticus

Taxonomic identifier

471 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	801 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes an exceptionally high rate of oxidation of a wide range of aldose sugars, including D-glucose, galactose, arabinose and xylose, and also the disaccharides lactose, cellobiose and maltose.
Catalytic activity	D-glucose + acceptor = D-glucono-1,5-lactone + reduced acceptor.
Cofactor	PQQ.
Subunit structure	Monomer.
Subcellular location	Cell inner membrane; Multi-pass membrane protein; Periplasmic side.
Miscellaneous	Acinetobacter calcoaceticus contains two different PQQ dependent enzymes with GDH activity. GDH-A prefers 2-deoxyglucose as substrate, the specific substrates for GDH-B are disaccharides.
Sequence similarities	Belongs to the bacterial PQQ dehydrogenase family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	PQQ
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on CH-OH group of donors Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 801	768	Quinoprotein glucose dehydrogenase A		PRO_0000025557
Regions
	Transmembrane	39 – 55	17	Helical; Potential
	Transmembrane	59 – 79	21	Helical; Potential
	Transmembrane	94 – 108	15	Helical; Potential
	Transmembrane	119 – 138	20	Helical; Potential
Sites
	Active site	471	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	P05465 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 2F4EA222EB5450D8	FASTA	801	86,957
	10 20 30 40 50 60 MNQPTSRSGL TTFTVIIIGL LALFLLIGGI WLATLGGSIY YIIAGVLLLI VAWQLYKRAS 70 80 90 100 110 120 TALWFYAALM LGTIIWSVWE VGTDFWALAP RLDILGILGL WLLVPAVTRG INNLGSSKVA 130 140 150 160 170 180 LSSTLAIAIV LMVYSIFNDP QEINGEIKTP QPETAQAVPG VAESDWPAYG RTQAGVRYSP 190 200 210 220 230 240 LKQINDQNVK DLKVAWTLRT GDLKTDNDSG ETTNQVTPIK IGNNMFICTA HQQLIAIDPA 250 260 270 280 290 300 TGKEKWRFDP KLKTDKSFQH LTCRGVMYYD ANNTTEFATS LQSKKSSSTQ CPRKVFVPVN 310 320 330 340 350 360 DGRLVAVNAD TGKACTDFGQ NGQVNLQEFM PYAYPGGYNP TSPGIVTGST VVIAGSVTDN 370 380 390 400 410 420 YSNKEPSGVI RGYDVNTGKL LWVFDTGAAD PNAMPGEGTT FVHNSPNAWA PLAYDAKLDI 430 440 450 460 470 480 VYVPTGVGTP DIWGGDRTEL KERYANSMLA INASTGKLVW NFQTTHHDLW DMDVPSQPSL 490 500 510 520 530 540 ADIKNKAGQT VPAIYVLTKT GNAFVLDRRN GQPIVPVTEK PVPQTVKRGP QTKGEFYSKT 550 560 570 580 590 600 QPFSDLNLAP QDKLTDKDMW GATMLDQLMC RVSFKRLNYD GIYTPPSENG TLVFPGNLGV 610 620 630 640 650 660 FEWGGMSVNP DRQVAVMNPI GLPFVSRLIP ADPNRAQTAK GAGTEQGVQP MYGVPYGVEI 670 680 690 700 710 720 SAFLSPLGLP CKQPAWGYVA GVDLKTHEVV WKKRIGTIRD SLPNLFQLPA VKIGVPGLGG 730 740 750 760 770 780 SISTAGNVMF VGATQDNYLR AFNVTNGKKL WEARLPAGGQ ATPMTYEING KQYVVIMAGG 790 800 HGSFGTKMGD YLVAYALPDN K

References

[1]

"Nucleotide sequence of the gene coding for quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus." Cleton-Jansen A.-M., Goosen N., Odle G., van de Putte P. Nucleic Acids Res. 16:6228-6228(1988) [PubMed: 3399393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LMD 79.41.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07235 Genomic DNA. Translation: CAA30222.1.
PIR	S00943.
3D structure databases
ProteinModelPortal	P05465.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017511. PQQ-dep_membr-bd_DH. IPR019556. PQQ-dependent_C. IPR019551. PQQ-dependent_N. IPR018391. PQQ_beta_propeller_repeat. IPR002372. PQQ_repeat. IPR011047. Quinonprotein_ADH-like. IPR001479. Quinoprotein_DH_CS. [Graphical view]
Gene3D	G3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 2 hits.
Pfam	PF01011. PQQ. 6 hits. PF10535. PQQ_C. 1 hit. PF10527. PQQ_N. 1 hit. [Graphical view]
SMART	SM00564. PQQ. 5 hits. [Graphical view]
SUPFAM	SSF50998. Quin_alc_DH_like. 1 hit.
TIGRFAMs	TIGR03074. PQQ_membr_DH. 1 hit.
PROSITE	PS00363. BACTERIAL_PQQ_1. 1 hit. PS00364. BACTERIAL_PQQ_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHGA_ACICA

Accession

Primary (citable) accession number: P05465

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	July 27, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program