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P05457 (GLNA1_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase 1

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase I
Glutamine synthetase I
Short name=GSI
Gene names
Name:glnA
Synonyms:glnI
Ordered Locus Names:blr4949
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulation

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.

Subunit structure

Oligomer of 12 subunits arranged in the form of two hexagons.

Subcellular location

Cytoplasm.

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Glutamine synthetase 1
PRO_0000153219

Amino acid modifications

Modified residue3981O-AMP-tyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
P05457 [UniParc].

Last modified February 28, 2003. Version 2.
Checksum: DCFA18CAA64F4B80

FASTA46952,315
        10         20         30         40         50         60 
MKTAKDVLKS IKDNDVKYVD LRFTDPRGKW QHVTFDVSMI DEDIFAEGTM FDGSSIAGWK 

        70         80         90        100        110        120 
AINESDMCLM PDPVTATIDP FFAETTMVIT CDVLEPTTGE PYNRDPRGIA KKAEAMVKSM 

       130        140        150        160        170        180 
GVGDTVFVGP EAEFFVFDDV RFSSSPYNTG FRLDSSELPT NTDTEYEGGN LGHRVRTKGG 

       190        200        210        220        230        240 
YFPVPPQDSV QDMRSEMLGA MAKMGVKVEK HHHEVASAQH ELGMKFDTLT LMADHLQIYK 

       250        260        270        280        290        300 
YCIHQVAHIY GKTATFMPKP VYGDNGSGMH VHQSIWKDGK PVFAGNKYAD LSETCLHYIG 

       310        320        330        340        350        360 
GIIKHAKAIN AFTNPSTNSY KRLVPGYEAP VLLAYSARNR SASCRIPYTA SPKAKRVEVR 

       370        380        390        400        410        420 
FPDPLANPYL GFAAMLMAGL DGIKNKIDPG PAMDKDLYDL PKEELKQIPT VCGSLREALE 

       430        440        450        460 
NLDKDRAFLK AGGVFDDDFI DSYIELKMTE VERFEMTPHP VEFDMYYSG 

« Hide

References

« Hide 'large scale' references
[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.
[2]"Characterization of the gene encoding glutamine synthetase I (glnA) from Bradyrhizobium japonicum."
Carlson T.A., Guerinot M.L., Chelm B.K.
J. Bacteriol. 162:698-703(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
[3]"Bradyrhizobium japonicum glnB, a putative nitrogen-regulatory gene, is regulated by NtrC at tandem promoters."
Martin G.B., Thomashow M.F., Chelm B.K.
J. Bacteriol. 171:5638-5645(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC50214.1.
M10926 Genomic DNA. Translation: AAA26213.1.
M26753 Genomic DNA. Translation: AAA26215.1.
PIRB33600.
RefSeqNP_771589.1. NC_004463.1.

3D structure databases

ProteinModelPortalP05457.
SMRP05457. Positions 3-468.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.blr4949.

Proteomic databases

PRIDEP05457.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC50214; BAC50214; BAC50214.
GeneID1051981.
KEGGbja:blr4949.
PATRIC21193582. VBIBraJap65052_5037.

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000005157.
KOK01915.
OMAISWARRN.
OrthoDBEOG6B360N.
ProtClustDBCLSK911295.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-4985-MONOMER.

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA1_BRADU
AccessionPrimary (citable) accession number: P05457
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 28, 2003
Last modified: December 11, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families