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P05457

- GLNA1_BRADU

UniProt

P05457 - GLNA1_BRADU

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Protein

Glutamine synthetase 1

Gene

glnA

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulationi

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-4985-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase 1 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase I
Glutamine synthetase I
Short name:
GSI
Gene namesi
Name:glnA
Synonyms:glnI
Ordered Locus Names:blr4949
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Glutamine synthetase 1PRO_0000153219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei398 – 3981O-AMP-tyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP05457.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.

Protein-protein interaction databases

STRINGi224911.blr4949.

Structurei

3D structure databases

ProteinModelPortaliP05457.
SMRiP05457. Positions 3-468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000005157.
InParanoidiP05457.
KOiK01915.
OrthoDBiEOG6B360N.
PhylomeDBiP05457.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05457-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTAKDVLKS IKDNDVKYVD LRFTDPRGKW QHVTFDVSMI DEDIFAEGTM
60 70 80 90 100
FDGSSIAGWK AINESDMCLM PDPVTATIDP FFAETTMVIT CDVLEPTTGE
110 120 130 140 150
PYNRDPRGIA KKAEAMVKSM GVGDTVFVGP EAEFFVFDDV RFSSSPYNTG
160 170 180 190 200
FRLDSSELPT NTDTEYEGGN LGHRVRTKGG YFPVPPQDSV QDMRSEMLGA
210 220 230 240 250
MAKMGVKVEK HHHEVASAQH ELGMKFDTLT LMADHLQIYK YCIHQVAHIY
260 270 280 290 300
GKTATFMPKP VYGDNGSGMH VHQSIWKDGK PVFAGNKYAD LSETCLHYIG
310 320 330 340 350
GIIKHAKAIN AFTNPSTNSY KRLVPGYEAP VLLAYSARNR SASCRIPYTA
360 370 380 390 400
SPKAKRVEVR FPDPLANPYL GFAAMLMAGL DGIKNKIDPG PAMDKDLYDL
410 420 430 440 450
PKEELKQIPT VCGSLREALE NLDKDRAFLK AGGVFDDDFI DSYIELKMTE
460
VERFEMTPHP VEFDMYYSG
Length:469
Mass (Da):52,315
Last modified:February 28, 2003 - v2
Checksum:iDCFA18CAA64F4B80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC50214.1.
M10926 Genomic DNA. Translation: AAA26213.1.
M26753 Genomic DNA. Translation: AAA26215.1.
PIRiB33600.
RefSeqiNP_771589.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC50214; BAC50214; BAC50214.
GeneIDi1051981.
KEGGibja:blr4949.
PATRICi21193582. VBIBraJap65052_5037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC50214.1 .
M10926 Genomic DNA. Translation: AAA26213.1 .
M26753 Genomic DNA. Translation: AAA26215.1 .
PIRi B33600.
RefSeqi NP_771589.1. NC_004463.1.

3D structure databases

ProteinModelPortali P05457.
SMRi P05457. Positions 3-468.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr4949.

Proteomic databases

PRIDEi P05457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC50214 ; BAC50214 ; BAC50214 .
GeneIDi 1051981.
KEGGi bja:blr4949.
PATRICi 21193582. VBIBraJap65052_5037.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000005157.
InParanoidi P05457.
KOi K01915.
OrthoDBi EOG6B360N.
PhylomeDBi P05457.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-4985-MONOMER.

Family and domain databases

Gene3Di 3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
TIGRFAMsi TIGR00653. GlnA. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.
  2. "Characterization of the gene encoding glutamine synthetase I (glnA) from Bradyrhizobium japonicum."
    Carlson T.A., Guerinot M.L., Chelm B.K.
    J. Bacteriol. 162:698-703(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
  3. "Bradyrhizobium japonicum glnB, a putative nitrogen-regulatory gene, is regulated by NtrC at tandem promoters."
    Martin G.B., Thomashow M.F., Chelm B.K.
    J. Bacteriol. 171:5638-5645(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.

Entry informationi

Entry nameiGLNA1_BRADU
AccessioniPrimary (citable) accession number: P05457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 28, 2003
Last modified: October 29, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3