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P05457

- GLNA1_BRADU

UniProt

P05457 - GLNA1_BRADU

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Protein
Glutamine synthetase 1
Gene
glnA, glnI, blr4949
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulationi

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-4985-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase 1 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase I
Glutamine synthetase I
Short name:
GSI
Gene namesi
Name:glnA
Synonyms:glnI
Ordered Locus Names:blr4949
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Glutamine synthetase 1
PRO_0000153219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei398 – 3981O-AMP-tyrosine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP05457.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.

Protein-protein interaction databases

STRINGi224911.blr4949.

Structurei

3D structure databases

ProteinModelPortaliP05457.
SMRiP05457. Positions 3-468.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000005157.
KOiK01915.
OrthoDBiEOG6B360N.
PhylomeDBiP05457.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05457-1 [UniParc]FASTAAdd to Basket

« Hide

MKTAKDVLKS IKDNDVKYVD LRFTDPRGKW QHVTFDVSMI DEDIFAEGTM    50
FDGSSIAGWK AINESDMCLM PDPVTATIDP FFAETTMVIT CDVLEPTTGE 100
PYNRDPRGIA KKAEAMVKSM GVGDTVFVGP EAEFFVFDDV RFSSSPYNTG 150
FRLDSSELPT NTDTEYEGGN LGHRVRTKGG YFPVPPQDSV QDMRSEMLGA 200
MAKMGVKVEK HHHEVASAQH ELGMKFDTLT LMADHLQIYK YCIHQVAHIY 250
GKTATFMPKP VYGDNGSGMH VHQSIWKDGK PVFAGNKYAD LSETCLHYIG 300
GIIKHAKAIN AFTNPSTNSY KRLVPGYEAP VLLAYSARNR SASCRIPYTA 350
SPKAKRVEVR FPDPLANPYL GFAAMLMAGL DGIKNKIDPG PAMDKDLYDL 400
PKEELKQIPT VCGSLREALE NLDKDRAFLK AGGVFDDDFI DSYIELKMTE 450
VERFEMTPHP VEFDMYYSG 469
Length:469
Mass (Da):52,315
Last modified:February 28, 2003 - v2
Checksum:iDCFA18CAA64F4B80
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC50214.1.
M10926 Genomic DNA. Translation: AAA26213.1.
M26753 Genomic DNA. Translation: AAA26215.1.
PIRiB33600.
RefSeqiNP_771589.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC50214; BAC50214; BAC50214.
GeneIDi1051981.
KEGGibja:blr4949.
PATRICi21193582. VBIBraJap65052_5037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC50214.1 .
M10926 Genomic DNA. Translation: AAA26213.1 .
M26753 Genomic DNA. Translation: AAA26215.1 .
PIRi B33600.
RefSeqi NP_771589.1. NC_004463.1.

3D structure databases

ProteinModelPortali P05457.
SMRi P05457. Positions 3-468.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr4949.

Proteomic databases

PRIDEi P05457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC50214 ; BAC50214 ; BAC50214 .
GeneIDi 1051981.
KEGGi bja:blr4949.
PATRICi 21193582. VBIBraJap65052_5037.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000005157.
KOi K01915.
OrthoDBi EOG6B360N.
PhylomeDBi P05457.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-4985-MONOMER.

Family and domain databases

Gene3Di 3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
TIGRFAMsi TIGR00653. GlnA. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.
  2. "Characterization of the gene encoding glutamine synthetase I (glnA) from Bradyrhizobium japonicum."
    Carlson T.A., Guerinot M.L., Chelm B.K.
    J. Bacteriol. 162:698-703(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
  3. "Bradyrhizobium japonicum glnB, a putative nitrogen-regulatory gene, is regulated by NtrC at tandem promoters."
    Martin G.B., Thomashow M.F., Chelm B.K.
    J. Bacteriol. 171:5638-5645(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.

Entry informationi

Entry nameiGLNA1_BRADU
AccessioniPrimary (citable) accession number: P05457
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: February 28, 2003
Last modified: September 3, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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