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P05457

- GLNA1_BRADU

UniProt

P05457 - GLNA1_BRADU

Protein

Glutamine synthetase 1

Gene

glnA

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (28 Feb 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Enzyme regulationi

    The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-4985-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase 1 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase I
    Glutamine synthetase I
    Short name:
    GSI
    Gene namesi
    Name:glnA
    Synonyms:glnI
    Ordered Locus Names:blr4949
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Glutamine synthetase 1PRO_0000153219Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei398 – 3981O-AMP-tyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP05457.

    Interactioni

    Subunit structurei

    Oligomer of 12 subunits arranged in the form of two hexagons.

    Protein-protein interaction databases

    STRINGi224911.blr4949.

    Structurei

    3D structure databases

    ProteinModelPortaliP05457.
    SMRiP05457. Positions 3-468.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000005157.
    KOiK01915.
    OrthoDBiEOG6B360N.
    PhylomeDBiP05457.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR00653. GlnA. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05457-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTAKDVLKS IKDNDVKYVD LRFTDPRGKW QHVTFDVSMI DEDIFAEGTM    50
    FDGSSIAGWK AINESDMCLM PDPVTATIDP FFAETTMVIT CDVLEPTTGE 100
    PYNRDPRGIA KKAEAMVKSM GVGDTVFVGP EAEFFVFDDV RFSSSPYNTG 150
    FRLDSSELPT NTDTEYEGGN LGHRVRTKGG YFPVPPQDSV QDMRSEMLGA 200
    MAKMGVKVEK HHHEVASAQH ELGMKFDTLT LMADHLQIYK YCIHQVAHIY 250
    GKTATFMPKP VYGDNGSGMH VHQSIWKDGK PVFAGNKYAD LSETCLHYIG 300
    GIIKHAKAIN AFTNPSTNSY KRLVPGYEAP VLLAYSARNR SASCRIPYTA 350
    SPKAKRVEVR FPDPLANPYL GFAAMLMAGL DGIKNKIDPG PAMDKDLYDL 400
    PKEELKQIPT VCGSLREALE NLDKDRAFLK AGGVFDDDFI DSYIELKMTE 450
    VERFEMTPHP VEFDMYYSG 469
    Length:469
    Mass (Da):52,315
    Last modified:February 28, 2003 - v2
    Checksum:iDCFA18CAA64F4B80
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC50214.1.
    M10926 Genomic DNA. Translation: AAA26213.1.
    M26753 Genomic DNA. Translation: AAA26215.1.
    PIRiB33600.
    RefSeqiNP_771589.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC50214; BAC50214; BAC50214.
    GeneIDi1051981.
    KEGGibja:blr4949.
    PATRICi21193582. VBIBraJap65052_5037.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC50214.1 .
    M10926 Genomic DNA. Translation: AAA26213.1 .
    M26753 Genomic DNA. Translation: AAA26215.1 .
    PIRi B33600.
    RefSeqi NP_771589.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali P05457.
    SMRi P05457. Positions 3-468.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.blr4949.

    Proteomic databases

    PRIDEi P05457.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC50214 ; BAC50214 ; BAC50214 .
    GeneIDi 1051981.
    KEGGi bja:blr4949.
    PATRICi 21193582. VBIBraJap65052_5037.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000005157.
    KOi K01915.
    OrthoDBi EOG6B360N.
    PhylomeDBi P05457.

    Enzyme and pathway databases

    BioCyci BJAP224911:GJEJ-4985-MONOMER.

    Family and domain databases

    Gene3Di 3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    TIGRFAMsi TIGR00653. GlnA. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.
    2. "Characterization of the gene encoding glutamine synthetase I (glnA) from Bradyrhizobium japonicum."
      Carlson T.A., Guerinot M.L., Chelm B.K.
      J. Bacteriol. 162:698-703(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
    3. "Bradyrhizobium japonicum glnB, a putative nitrogen-regulatory gene, is regulated by NtrC at tandem promoters."
      Martin G.B., Thomashow M.F., Chelm B.K.
      J. Bacteriol. 171:5638-5645(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.

    Entry informationi

    Entry nameiGLNA1_BRADU
    AccessioniPrimary (citable) accession number: P05457
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: February 28, 2003
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3