Reviewed, UniProtKB/Swiss-Prot P05423 (RPC4_HUMAN)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase III subunit RPC4
      Short name=RNA polymerase III subunit C4
Alternative name(s):
    DNA-directed RNA polymerase III subunit D
    DNA-directed RNA polymerase III 47 kDa polypeptide
    RPC53
    Protein BN51

Gene names

Name:	POLR3D
Synonyms:	BN51, BN51T

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity. Interacts with POLR3E/RPC5.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the eukaryotic RPC4/POLR3D RNA polymerase subunit family.
Sequence caution	The sequence BC000516 differs from that shown. Reason: Frameshift at position 175. The sequence BC003039 differs from that shown. Reason: Frameshift at position 175.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	transcription from RNA polymerase III promoter Inferred from electronic annotation. Source: InterPro
Cellular component	DNA-directed RNA polymerase III complex Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	DNA-directed RNA polymerase III subunit RPC4		PRO_0000073967
Amino acid modifications
	Modified residue	386	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	9 – 18	10	EPSTPGGPRP → RPARQGPDL in AAA51838. Ref.1
	Sequence conflict	9 – 12	4	EPST → RPAR in AAA72377. Ref.6
	Sequence conflict	26 – 40	15	LIGRR…TPGRL → SSGGGGLPSPPAV Ref.1
	Sequence conflict	98	1	G → R in AAA51838. Ref.1
	Sequence conflict	233	1	K → R in AAM18216. Ref.2
	Sequence conflict	286	1	P → L in AAM18216. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05423-1 [UniParc]. Last modified March 28, 2003. Version 2. Checksum: CD8AFF3257B78410	FASTA	398	44,396
	10 20 30 40 50 60 MSEGNAAGEP STPGGPRPLL TGARGLIGRR PAPPLTPGRL PSIRSRDLTL GGVKKKTFTP 70 80 90 100 110 120 NIISRKIKEE PKEEVTVKKE KRERDRDRQR EGHGRGRGRP EVIQSHSIFE QGPAEMMKKK 130 140 150 160 170 180 GNWDKTVDVS DMGPSHIINI KKEKRETDEE TKQILRMLEK DDFLDDPGLR NDTRNMPVQL 190 200 210 220 230 240 PLAHSGWLFK EENDEPDVKP WLAGPKEEDM EVDIPAVKVK EEPRDEEEEA KMKAPPKAAR 250 260 270 280 290 300 KTPGLPKDVS VAELLRELSL TKEEELLFLQ LPDTLPGQPP TQDIKPIKTE VQGEDGQVVL 310 320 330 340 350 360 IKQEKDREAK LAENACTLAD LTEGQVGKLL IRKSGRVQLL LGKVTLDVTM GTACSFLQEL 370 380 390 VSVGLGDSRT GEMTVLGHVK HKLVCSPDFE SLLDHKHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells." Ittmann M., Greco A., Basilico C. Mol. Cell. Biol. 7:3386-3393(1987) [PubMed: 3683386] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits." Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N. Mol. Cell. Biol. 22:8044-8055(2002) [PubMed: 12391170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN THE RNA POL III COMPLEX, MASS SPECTROMETRY, INTERACTION WITH POLR3E.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Lymph.
[5]	"Reconstitution of transcription from the human U6 small nuclear RNA promoter with eight recombinant polypeptides and a partially purified RNA polymerase III complex." Chong S.S., Hu P., Hernandez N. J. Biol. Chem. 276:20727-20734(2001) [PubMed: 11279001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-50.
[6]	"Promoter structure and cell cycle control of the BN51 cell cycle gene, which encodes a subunit of RNA polymerase III." Ittmann M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
[7]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-386, MASS SPECTROMETRY. Tissue: Epithelium.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17754 mRNA. Translation: AAA51838.1. AY092086 mRNA. Translation: AAM18216.1. CR536509 mRNA. Translation: CAG38747.1. CR541803 mRNA. Translation: CAG46602.1. BC000516 mRNA. No translation available. BC003039 mRNA. No translation available. BC002603 mRNA. Translation: AAH02603.1. BC004484 mRNA. Translation: AAH04484.1. AF346574 mRNA. Translation: AAK15371.1. L15301 Genomic DNA. Translation: AAA72377.1.
IPI	IPI00215978.
PIR	A43700.
RefSeq	NP_001713.2.
UniGene	Hs.148342
3D structure databases
ModBase	Search...
Proteomic databases
PeptideAtlas	P05423.
PRIDE	P05423.
Genome annotation databases
Ensembl	ENSG00000168495. Homo sapiens. [Contig view]
GeneID	661.
KEGG	hsa:661.
NMPDR	fig|9606.3.peg.30039.
Organism-specific databases
GeneCards	GC08P022158.
H-InvDB	HIX0007368.
HGNC	HGNC:1080. POLR3D.
MIM	187280. gene.
PharmGKB	PA25390.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P05423.
HOVERGEN	P05423.
OMA	P05423. DVTMGTT.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription.
Gene expression databases
ArrayExpress	P05423.
Bgee	P05423.
CleanEx	HS_POLR3D.
GermOnline	ENSG00000168495. Homo sapiens.
Family and domain databases
InterPro	IPR007811. RNA_pol_Rpc4. [Graphical view]
PANTHER	PTHR13408. RNA_pol_Rpc4. 1 hit.
Pfam	PF05132. RNA_pol_Rpc4. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	2690.
SOURCE	Search...

Entry information

Entry name

RPC4_HUMAN

Accession

Primary (citable) accession number: P05423
Secondary accession number(s): Q6FI28 , Q9BPV7, Q9BPZ1, Q9BXB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	March 28, 2003
Last modified:	June 16, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)