P05423 (RPC4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase III subunit RPC4
Short name=RNA polymerase III subunit C4

Alternative name(s):
DNA-directed RNA polymerase III subunit D
Protein BN51
RNA polymerase III 47 kDa subunit
RPC53 homolog

Gene names

Name:	POLR3D
Synonyms:	BN51, BN51T

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway By similarity. Ref.7 Ref.8
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity. Interacts with POLR3E/RPC5. Ref.2
Subcellular location	Nucleus.
Sequence similarities	Belongs to the eukaryotic RPC4/POLR3D RNA polymerase subunit family.
Sequence caution	The sequence BC000516 differs from that shown. Reason: Frameshift at position 175. The sequence BC003039 differs from that shown. Reason: Frameshift at position 175.

Ontologies

Keywords
Biological process	Antiviral defense Immunity Innate immunity Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW innate immune response Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of innate immune response Inferred from direct assay Ref.7. Source: UniProtKB positive regulation of interferon-beta production Inferred from direct assay Ref.7. Source: UniProtKB termination of RNA polymerase III transcription Traceable author statement. Source: Reactome transcription elongation from RNA polymerase III promoter Traceable author statement. Source: Reactome
Cellular_component	DNA-directed RNA polymerase III complex Inferred from electronic annotation. Source: InterPro nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW chromatin binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	DNA-directed RNA polymerase III subunit RPC4		PRO_0000073967
Experimental info
	Sequence conflict	9 – 18	10	EPSTPGGPRP → RPARQGPDL in AAA51838. Ref.1
	Sequence conflict	9 – 12	4	EPST → RPAR in AAA72377. Ref.6
	Sequence conflict	26 – 40	15	LIGRR…TPGRL → SSGGGGLPSPPAV Ref.1
	Sequence conflict	98	1	G → R in AAA51838. Ref.1
	Sequence conflict	233	1	K → R in AAM18216. Ref.2
	Sequence conflict	286	1	P → L in AAM18216. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05423 [UniParc]. Last modified March 28, 2003. Version 2. Checksum: CD8AFF3257B78410	FASTA	398	44,396
	10 20 30 40 50 60 MSEGNAAGEP STPGGPRPLL TGARGLIGRR PAPPLTPGRL PSIRSRDLTL GGVKKKTFTP 70 80 90 100 110 120 NIISRKIKEE PKEEVTVKKE KRERDRDRQR EGHGRGRGRP EVIQSHSIFE QGPAEMMKKK 130 140 150 160 170 180 GNWDKTVDVS DMGPSHIINI KKEKRETDEE TKQILRMLEK DDFLDDPGLR NDTRNMPVQL 190 200 210 220 230 240 PLAHSGWLFK EENDEPDVKP WLAGPKEEDM EVDIPAVKVK EEPRDEEEEA KMKAPPKAAR 250 260 270 280 290 300 KTPGLPKDVS VAELLRELSL TKEEELLFLQ LPDTLPGQPP TQDIKPIKTE VQGEDGQVVL 310 320 330 340 350 360 IKQEKDREAK LAENACTLAD LTEGQVGKLL IRKSGRVQLL LGKVTLDVTM GTACSFLQEL 370 380 390 VSVGLGDSRT GEMTVLGHVK HKLVCSPDFE SLLDHKHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the human gene that complements a temperature-sensitive cell cycle mutation in BHK cells." Ittmann M., Greco A., Basilico C. Mol. Cell. Biol. 7:3386-3393(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits." Hu P., Wu S., Sun Y., Yuan C.-C., Kobayashi R., Myers M.P., Hernandez N. Mol. Cell. Biol. 22:8044-8055(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION IN THE RNA POL III COMPLEX, MASS SPECTROMETRY, INTERACTION WITH POLR3E.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Lymph.
[5]	"Reconstitution of transcription from the human U6 small nuclear RNA promoter with eight recombinant polypeptides and a partially purified RNA polymerase III complex." Chong S.S., Hu P., Hernandez N. J. Biol. Chem. 276:20727-20734(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-50.
[6]	"Promoter structure and cell cycle control of the BN51 cell cycle gene, which encodes a subunit of RNA polymerase III." Ittmann M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-12.
[7]	"RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway." Chiu Y.-H., Macmillan J.B., Chen Z.J. Cell 138:576-591(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate." Ablasser A., Bauernfeind F., Hartmann G., Latz E., Fitzgerald K.A., Hornung V. Nat. Immunol. 10:1065-1072(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M17754 mRNA. Translation: AAA51838.1. AY092086 mRNA. Translation: AAM18216.1. CR536509 mRNA. Translation: CAG38747.1. CR541803 mRNA. Translation: CAG46602.1. BC000516 mRNA. No translation available. BC003039 mRNA. No translation available. BC002603 mRNA. Translation: AAH02603.1. BC004484 mRNA. Translation: AAH04484.1. AF346574 mRNA. Translation: AAK15371.1. L15301 Genomic DNA. Translation: AAA72377.1.
IPI	IPI00215978.
PIR	A43700.
RefSeq	NP_001713.2. NM_001722.2.
UniGene	Hs.148342.
3D structure databases
ProteinModelPortal	P05423.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-56155N.
IntAct	P05423. 1 interaction.
MINT	MINT-3004820.
STRING	9606.ENSP00000303088.
PTM databases
PhosphoSite	P05423.
Polymorphism databases
DMDM	29429159.
Proteomic databases
PaxDb	P05423.
PeptideAtlas	P05423.
PRIDE	P05423.
Protocols and materials databases
DNASU	661.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306433; ENSP00000303088; ENSG00000168495. ENST00000397802; ENSP00000380904; ENSG00000168495.
GeneID	661.
KEGG	hsa:661.
UCSC	uc003xbl.3. human.
Organism-specific databases
CTD	661.
GeneCards	GC08P022102.
HGNC	HGNC:1080. POLR3D.
MIM	187280. gene.
neXtProt	NX_P05423.
PharmGKB	PA25390.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG239914.
HOGENOM	HOG000008080.
HOVERGEN	HBG039777.
InParanoid	P05423.
KO	K03026.
OMA	GTTCSFL.
OrthoDB	EOG4KSPKB.
PhylomeDB	P05423.
Enzyme and pathway databases
Reactome	REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P05423.
Bgee	P05423.
CleanEx	HS_POLR3D.
Genevestigator	P05423.
GermOnline	ENSG00000168495. Homo sapiens.
Family and domain databases
InterPro	IPR007811. RNA_pol_III_Rpc4. [Graphical view]
PANTHER	PTHR13408. PTHR13408. 1 hit.
Pfam	PF05132. RNA_pol_Rpc4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P05423.
ChEMBL	CHEMBL2947.
GenomeRNAi	661.
NextBio	2690.
SOURCE	Search...

Entry information

Entry name

RPC4_HUMAN

Accession

Primary (citable) accession number: P05423
Secondary accession number(s): Q6FI28 , Q9BPV7, Q9BPZ1, Q9BXB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	March 28, 2003
Last modified:	May 29, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.