P05417 (UCRI_PARDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquinol-cytochrome c reductase iron-sulfur subunit
EC=1.10.2.2

Alternative name(s):
Rieske iron-sulfur protein
Short name=RISP

Gene names

Name:

petA

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell membrane; Single-pass membrane protein.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Ubiquinol-cytochrome c reductase iron-sulfur subunit		PRO_0000127761
Regions
	Transmembrane	18 – 39	22	Helical; Potential
	Domain	95 – 188	94	Rieske
Sites
	Metal binding	132	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	134	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	152	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	155	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	137 ↔ 154		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05417 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: F83F5D9A9C1FBEE4	FASTA	190	20,300
	10 20 30 40 50 60 MSHADEHAGD HGATRRDFLY YATAGAGTVA AGAAAWTLVN QMNPSADVQA LASIQVDVSG 70 80 90 100 110 120 VETGTQLTVK WLGKPVFIRR RTEDEIQAGR EVDLGQLIDR SAQNSNKPDA PATDENRTMD 130 140 150 160 170 180 EAGEWLVMIG VCTHLGCVPI GDGAGDFGGW FCPCHGSHYD TSGRIRRGPA PQNLHIPVAE 190 FLDDTTIKLG

References

[1]	"The genes of the Paracoccus denitrificans bc1 complex. Nucleotide sequence and homologies between bacterial and mitochondrial subunits." Kurowski B., Ludwig B. J. Biol. Chem. 262:13805-13811(1987) [PubMed: 2820981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Immunoelectron microscopy and epitope mapping with monoclonal antibodies suggest the existence of an additional N-terminal transmembrane helix in the cytochrome b subunit of bacterial ubiquinol:cytochrome-c oxidoreductases." Kleymann G., Iwata S., Wiesmueller K.-H., Ludwig B., Haase W., Michel H. Eur. J. Biochem. 230:359-363(1995) [PubMed: 7601123] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-23 AND 37-60.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17522 Genomic DNA. Translation: AAA25571.1.
X05799 Genomic DNA. Translation: CAA29243.1.

PIR

A29413.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YIU	X-ray	2.70	C/F	1-190	[»]

ProteinModelPortal

P05417.

SMR

P05417. Positions 15-190.

ModBase

Search...

Protein family/group databases

TCDB

3.D.3.1.1. proton-translocating quinol:cytochrome c reductase (QCR) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
[Graphical view]

Gene3D

G3DSA:2.102.10.10. Rieske_reg. 1 hit.

PANTHER

PTHR10134. Rieske. 1 hit.

Pfam

PF00355. Rieske. 1 hit.
PF10399. UCR_Fe-S_N. 1 hit.
[Graphical view]

PRINTS

PR00162. RIESKE.

SUPFAM

SSF50022. Rieske_dom. 1 hit.

TIGRFAMs

TIGR01416. Rieske_proteo. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.

PROSITE

PS51296. RIESKE. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

UCRI_PARDE

Accession

Primary (citable) accession number: P05417

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program