Reviewed, UniProtKB/Swiss-Prot P05417 (UCRI_PARDE)

Names and origin

Protein names

Recommended name:
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    EC=1.10.2.2
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP

Gene names

Name:

petA

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell membrane; Single-pass membrane protein.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 190	190	Ubiquinol-cytochrome c reductase iron-sulfur subunit		PRO_0000127761
Regions
	Transmembrane	18 – 39	22	Potential
	Domain	95 – 188	94	Rieske
Sites
	Metal binding	132	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	134	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	152	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	155	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	137 ↔ 154		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05417-1 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: F83F5D9A9C1FBEE4	FASTA	190	20,300
	10 20 30 40 50 60 MSHADEHAGD HGATRRDFLY YATAGAGTVA AGAAAWTLVN QMNPSADVQA LASIQVDVSG 70 80 90 100 110 120 VETGTQLTVK WLGKPVFIRR RTEDEIQAGR EVDLGQLIDR SAQNSNKPDA PATDENRTMD 130 140 150 160 170 180 EAGEWLVMIG VCTHLGCVPI GDGAGDFGGW FCPCHGSHYD TSGRIRRGPA PQNLHIPVAE 190 FLDDTTIKLG

References

[1]

"The genes of the Paracoccus denitrificans bc1 complex. Nucleotide sequence and homologies between bacterial and mitochondrial subunits." Kurowski B., Ludwig B. J. Biol. Chem. 262:13805-13811(1987) [PubMed: 2820981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

[2]

"Immunoelectron microscopy and epitope mapping with monoclonal antibodies suggest the existence of an additional N-terminal transmembrane helix in the cytochrome b subunit of bacterial ubiquinol:cytochrome-c oxidoreductases." Kleymann G., Iwata S., Wiesmueller K.-H., Ludwig B., Haase W., Michel H. Eur. J. Biochem. 230:359-363(1995) [PubMed: 7601123] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-23 AND 37-60.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17522 Genomic DNA. Translation: AAA25571.1. X05799 Genomic DNA. Translation: CAA29243.1.
PIR	A29413.
3D structure databases
HSSP	HSSP built from PDB template 1RIE based on UniProtKB P13272.
SMR	P05417. Positions 13-190.
ModBase	Search...
Protein family/group databases
TCDB	3.D.3.1.1. proton-translocating quinol:cytochrome c reductase (QCR) superfamily.
Enzyme and pathway databases
BRENDA	1.10.2.2. 59.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF10399. UCR_Fe-S_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_PARDE

Accession

Primary (citable) accession number: P05417

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)