Reviewed, UniProtKB/Swiss-Prot P05413 (FABPH_HUMAN)

Names and origin

Protein names

Recommended name:
    Fatty acid-binding protein, heart
Alternative name(s):
    Heart-type fatty acid-binding protein
      Short name=H-FABP
    Muscle fatty acid-binding protein
      Short name=M-FABP
    Mammary-derived growth inhibitor
      Short name=MDGI

Gene names

Name:	FABP3
Synonyms:	FABP11, MDGI

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Subcellular location	Cytoplasm.
Domain	Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
Sequence similarities	Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm
Ligand	Lipid-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of cell proliferation Traceable author statement. Source: ProtInc transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	lipid binding Ref.9 Traceable author statement. Source: ProtInc transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.9 Ref.10

Chain

2 – 133

132

Fatty acid-binding protein, heart

PRO_0000067321

Sites

Binding site

127

1

Fatty acid

Binding site

129

1

Fatty acid

Amino acid modifications

Modified residue

2

1

N-acetylvaline Ref.9

Modified residue

20

1

Phosphotyrosine; by Tyr-kinases By similarity

Experimental info

Sequence conflict

2

1

V → A in CAA71305. Ref.2

Sequence conflict

105

1

L → K AA sequence Ref.9

Sequence conflict

125

1

C → S AA sequence Ref.9

Sequence conflict

130

1

E → Q AA sequence Ref.10

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

133

Helix Strand Turn

Details...

Helix

3 – 5

3

Beta strand

7 – 16

10

Helix

17 – 24

8

Helix

28 – 34

7

Beta strand

40 – 46

7

Beta strand

49 – 55

7

Beta strand

61 – 65

5

Beta strand

71 – 74

4

Beta strand

80 – 88

9

Beta strand

91 – 98

8

Beta strand

101 – 110

10

Beta strand

113 – 120

8

Beta strand

123 – 131

9

Sequences

	Sequence		Length	Mass (Da)
	P05413-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 5FD396B1BE538A3C	FASTA	133	14,858
	10 20 30 40 50 60 MVDAFLGTWK LVDSKNFDDY MKSLGVGFAT RQVASMTKPT TIIEKNGDIL TLKTHSTFKN 70 80 90 100 110 120 TEISFKLGVE FDETTADDRK VKSIVTLDGG KLVHLQKWDG QETTLVRELI DGKLILTLTH 130 GTAVCTRTYE KEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the cDNA encoding human skeletal-muscle fatty-acid-binding protein, its peptide sequence and chromosomal localization." Peeter R.A., Veerkamp J.H., Kanda T., Ono T., Geurts van Kessel A. Biochem. J. 276:203-207(1991) [PubMed: 1710107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"Molecular cloning of human mammary-derived growth inhibitor (MDGI) reveals that its expression is associated with breast differentiation, but not with cancer progression." Hu Y.F., Ao X., Russo I.H., Russo J. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland.
[3]	"Genomic organization and complete nucleotide sequence of the human cardiac fatty acid binding protein gene (FABP3), and identification of a closely related genomic sequence." Wu X., Arlt M., Goodfellow P.J., Rottman J.N. Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[9]	"Characterization and amino acid sequence of a fatty acid-binding protein from human heart." Offner G.D., Brecher P., Sawlivich W.B., Costello C.E., Troxler R.F. Biochem. J. 252:191-198(1988) [PubMed: 3421901] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-133.
[10]	"Revision of the amino acid sequence of human heart fatty acid-binding protein." Boerchers T., Hoejrup P., Nielsen S.U., Roepstorff P., Spener F., Knudsen J. Mol. Cell. Biochem. 98:127-133(1990) [PubMed: 2266954] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-133, SEQUENCE REVISION.
[11]	"Localization of the gene for human heart fatty acid binding protein to chromosome 1p32-1p33." Troxler R.F., Offner G.D., Jiang J.W., Wu B.L., Skare J.C., Milunsky A., Wyandt H.E. Hum. Genet. 92:563-566(1993) [PubMed: 8262516] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-133. Tissue: Heart.
[12]	Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 23-31; 46-53; 67-80; 98-107 AND 114-127, MASS SPECTROMETRY. Tissue: Fetal brain cortex.
[13]	"The major protein expression profile and two-dimensional protein database of human heart." Kovalyov L.I., Shishkin S.S., Efimochkin A.S., Kovalyova M.A., Ershova E.S., Egorov T.A., Musalyamov A.K. Electrophoresis 16:1160-1169(1995) [PubMed: 7498159] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-39. Tissue: Heart.
[14]	"Three-dimensional structure of recombinant human muscle fatty acid-binding protein." Zanotti G., Scapin G., Spadon P., Veerkamp J.H., Sacchettini J.C. J. Biol. Chem. 267:18541-18550(1992) [PubMed: 1526991] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[15]	"Structural studies on human muscle fatty acid binding protein at 1.4-A resolution: binding interactions with three C18 fatty acids." Young A.C.M., Scapin G., Kromminga A., Patel S.B., Veerkamp J.H., Sacchettini J.C. Structure 2:523-534(1994) [PubMed: 7922029] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) IN COMPLEX WITH C18 FATTY ACID.
[16]	"Spin-system heterogeneities indicate a selected-fit mechanism in fatty acid binding to heart-type fatty acid-binding protein (H-FABP)." Luecke C., Rademacher M., Zimmerman A.W., van Moerkerk H.T.B., Veerkamp J.H., Rueterjans H. Biochem. J. 354:259-266(2001) [PubMed: 11171102] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X56549 mRNA. Translation: CAA39889.1.
Y10255 mRNA. Translation: CAA71305.1.
U57623 Genomic DNA. Translation: AAB02555.1.
AK314122 mRNA. Translation: BAG36813.1.
U17081 Genomic DNA. Translation: AAC99800.1.
S67314 mRNA. Translation: AAB29294.1.
BT006727 mRNA. Translation: AAP35373.1.
AL451070 Genomic DNA. Translation: CAH71850.1.
CH471059 Genomic DNA. Translation: EAX07619.1.
BC007021 mRNA. Translation: AAH07021.1.

IPI

IPI00219684.

PIR

FZHUC. S15432.

RefSeq

NP_004093.1.

UniGene

Hs.657242

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G5W	NMR	-	A	2-133	[»]
1HMR	X-ray	1.40	A	2-133	[»]
1HMS	X-ray	1.40	A	2-133	[»]
1HMT	X-ray	1.40	A	2-133	[»]
2HMB	X-ray	2.10	A	2-133	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P05413. 3 interactions.

STRING

P05413.

PTM databases

PhosphoSite

P05413.

2-D gel databases

HSC-2DPAGE

P05413.

REPRODUCTION-2DPAGE

IPI00219684.

Proteomic databases

PeptideAtlas

P05413.

PRIDE

P05413.

Genome annotation databases

Ensembl

ENST00000373713; ENSP00000362817; ENSG00000121769; Homo sapiens. [Genome view]

GeneID

2170.

KEGG

hsa:2170.

UCSC

uc001bss.1. human.

Organism-specific databases

CTD

2170.

GeneCards

GC01M031633.

H-InvDB

HIX0000353.

HGNC

HGNC:3557. FABP3.

HPA

CAB017830.

MIM

134651. gene.

PharmGKB

PA27958.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P05413.

HOVERGEN

P05413.

OMA

KWNGQET.

Gene expression databases

ArrayExpress

P05413.

Bgee

P05413.

CleanEx

HS_FABP3.

Genevestigator

P05413.

GermOnline

ENSG00000121769. Homo sapiens.

Family and domain databases

InterPro

IPR012674. Calycin.
IPR000463. Fatty_acid_bd.
IPR000566. Lipocln_cytFABP.
[Graphical view]

Gene3D

G3DSA:2.40.128.20. Calycin. 1 hit.

PANTHER

PTHR11955. Fatty_acid_bd. 1 hit.

Pfam

PF00061. Lipocalin. 1 hit.
[Graphical view]

PRINTS

PR00178. FATTYACIDBP.

PROSITE

PS00214. FABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

8763.

SOURCE

Search...

Entry information

Entry name

FABPH_HUMAN

Accession

Primary (citable) accession number: P05413
Secondary accession number(s): B2RAB6, Q5VV93, Q99957

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 106 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.