P05413 (FABPH_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fatty acid-binding protein, heart

Alternative name(s):
Fatty acid-binding protein 3
Heart-type fatty acid-binding protein
Short name=H-FABP
Mammary-derived growth inhibitor
Short name=MDGI
Muscle fatty acid-binding protein
Short name=M-FABP

Gene names

Name:	FABP3
Synonyms:	FABP11, MDGI

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
Subcellular location	Cytoplasm.
Domain	Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
Sequence similarities	Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	Lipid-binding
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of cell proliferation Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.9 Ref.10

Chain

2 – 133

132

Fatty acid-binding protein, heart

PRO_0000067321

Sites

Binding site

127

1

Fatty acid

Binding site

129

1

Fatty acid

Amino acid modifications

Modified residue

2

1

N-acetylvaline Ref.9

Modified residue

20

1

Phosphotyrosine; by Tyr-kinases By similarity

Natural variations

Natural variant

53

1

K → R.
Corresponds to variant rs2228194 [ dbSNP | Ensembl ].

VAR_061165

Experimental info

Sequence conflict

2

1

V → A in CAA71305. Ref.2

Sequence conflict

105

1

L → K AA sequence Ref.9

Sequence conflict

125

1

C → S AA sequence Ref.9

Sequence conflict

130

1

E → Q AA sequence Ref.10

Secondary structure

1

.

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133

Helix Strand Turn

Details...

Helix

3 – 5

3

Beta strand

7 – 16

10

Helix

17 – 24

8

Helix

28 – 34

7

Beta strand

40 – 46

7

Beta strand

49 – 55

7

Beta strand

61 – 65

5

Beta strand

71 – 74

4

Beta strand

80 – 88

9

Beta strand

91 – 98

8

Beta strand

101 – 110

10

Beta strand

113 – 120

8

Beta strand

123 – 131

9

Sequences

	Sequence		Length	Mass (Da)
	P05413 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 5FD396B1BE538A3C	FASTA	133	14,858
	10 20 30 40 50 60 MVDAFLGTWK LVDSKNFDDY MKSLGVGFAT RQVASMTKPT TIIEKNGDIL TLKTHSTFKN 70 80 90 100 110 120 TEISFKLGVE FDETTADDRK VKSIVTLDGG KLVHLQKWDG QETTLVRELI DGKLILTLTH 130 GTAVCTRTYE KEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the cDNA encoding human skeletal-muscle fatty-acid-binding protein, its peptide sequence and chromosomal localization." Peeter R.A., Veerkamp J.H., Kanda T., Ono T., Geurts van Kessel A. Biochem. J. 276:203-207(1991) [PubMed: 1710107] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"Molecular cloning of human mammary-derived growth inhibitor (MDGI) reveals that its expression is associated with breast differentiation, but not with cancer progression." Hu Y.F., Ao X., Russo I.H., Russo J. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Mammary gland.
[3]	"Genomic organization and complete nucleotide sequence of the human cardiac fatty acid binding protein gene (FABP3), and identification of a closely related genomic sequence." Wu X., Arlt M., Goodfellow P.J., Rottman J.N. Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hippocampus.
[6]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[9]	"Characterization and amino acid sequence of a fatty acid-binding protein from human heart." Offner G.D., Brecher P., Sawlivich W.B., Costello C.E., Troxler R.F. Biochem. J. 252:191-198(1988) [PubMed: 3421901] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-133.
[10]	"Revision of the amino acid sequence of human heart fatty acid-binding protein." Boerchers T., Hoejrup P., Nielsen S.U., Roepstorff P., Spener F., Knudsen J. Mol. Cell. Biochem. 98:127-133(1990) [PubMed: 2266954] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-133, SEQUENCE REVISION.
[11]	"Localization of the gene for human heart fatty acid binding protein to chromosome 1p32-1p33." Troxler R.F., Offner G.D., Jiang J.W., Wu B.L., Skare J.C., Milunsky A., Wyandt H.E. Hum. Genet. 92:563-566(1993) [PubMed: 8262516] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-133. Tissue: Heart.
[12]	Lubec G., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 23-31; 46-53; 67-80; 98-107 AND 114-127, MASS SPECTROMETRY. Tissue: Fetal brain cortex.
[13]	"The major protein expression profile and two-dimensional protein database of human heart." Kovalyov L.I., Shishkin S.S., Efimochkin A.S., Kovalyova M.A., Ershova E.S., Egorov T.A., Musalyamov A.K. Electrophoresis 16:1160-1169(1995) [PubMed: 7498159] [Abstract] Cited for: PROTEIN SEQUENCE OF 32-39. Tissue: Heart.
[14]	"Three-dimensional structure of recombinant human muscle fatty acid-binding protein." Zanotti G., Scapin G., Spadon P., Veerkamp J.H., Sacchettini J.C. J. Biol. Chem. 267:18541-18550(1992) [PubMed: 1526991] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
[15]	"Structural studies on human muscle fatty acid binding protein at 1.4-A resolution: binding interactions with three C18 fatty acids." Young A.C.M., Scapin G., Kromminga A., Patel S.B., Veerkamp J.H., Sacchettini J.C. Structure 2:523-534(1994) [PubMed: 7922029] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) IN COMPLEX WITH C18 FATTY ACID.
[16]	"Spin-system heterogeneities indicate a selected-fit mechanism in fatty acid binding to heart-type fatty acid-binding protein (H-FABP)." Luecke C., Rademacher M., Zimmerman A.W., van Moerkerk H.T.B., Veerkamp J.H., Rueterjans H. Biochem. J. 354:259-266(2001) [PubMed: 11171102] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X56549 mRNA. Translation: CAA39889.1.
Y10255 mRNA. Translation: CAA71305.1.
U57623 Genomic DNA. Translation: AAB02555.1.
AK314122 mRNA. Translation: BAG36813.1.
U17081 Genomic DNA. Translation: AAC99800.1.
S67314 mRNA. Translation: AAB29294.1.
BT006727 mRNA. Translation: AAP35373.1.
AL451070 Genomic DNA. Translation: CAH71850.1.
CH471059 Genomic DNA. Translation: EAX07619.1.
BC007021 mRNA. Translation: AAH07021.1.

IPI

IPI00219684.

PIR

FZHUC. S15432.

RefSeq

NP_004093.1. NM_004102.3.

UniGene

Hs.657242.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G5W	NMR	-	A	2-133	[»]
1HMR	X-ray	1.40	A	2-133	[»]
1HMS	X-ray	1.40	A	2-133	[»]
1HMT	X-ray	1.40	A	2-133	[»]
2HMB	X-ray	2.10	A	2-133	[»]

ProteinModelPortal

P05413.

SMR

P05413. Positions 2-132.

ModBase

Search...

Protein-protein interaction databases

IntAct

P05413. 10 interactions.

MINT

MINT-1432076.

STRING

P05413.

PTM databases

PhosphoSite

P05413.

Polymorphism databases

DMDM

119802.

2D gel databases

REPRODUCTION-2DPAGE

IPI00219684.

UCD-2DPAGE

P05413.

Proteomic databases

PeptideAtlas

P05413.

PRIDE

P05413.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000373713; ENSP00000362817; ENSG00000121769.

GeneID

2170.

KEGG

hsa:2170.

UCSC

uc001bss.1. human.

Organism-specific databases

CTD

2170.

GeneCards

GC01M031838.

H-InvDB

HIX0000353.

HGNC

HGNC:3557. FABP3.

HPA

CAB017830.

MIM

134651. gene.

neXtProt

NX_P05413.

PharmGKB

PA27958.

GenAtlas

Search...

Phylogenomic databases

eggNOG

maNOG19941.

HOGENOM

HBG714759.

HOVERGEN

HBG005633.

InParanoid

P05413.

OMA

VVCTRTY.

OrthoDB

EOG4P8FKD.

PhylomeDB

P05413.

Gene expression databases

ArrayExpress

P05413.

Bgee

P05413.

CleanEx

HS_FABP3.

Genevestigator

P05413.

GermOnline

ENSG00000121769. Homo sapiens.

Family and domain databases

InterPro

IPR012674. Calycin.
IPR011038. Calycin-like.
IPR000463. Fatty_acid-bd.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]

Gene3D

G3DSA:2.40.128.20. Calycin. 1 hit.

KO

K08752.

Pfam

PF00061. Lipocalin. 1 hit.
[Graphical view]

PRINTS

PR00178. FATTYACIDBP.

SUPFAM

SSF50814. Calycin. 1 hit.

PROSITE

PS00214. FABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

8763.

SOURCE

Search...

Entry information

Entry name

FABPH_HUMAN

Accession

Primary (citable) accession number: P05413
Secondary accession number(s): B2RAB6, Q5VV93, Q99957

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 125 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.