Reviewed,
UniProtKB/Swiss-Prot P05412 (JUN_HUMAN)
Last modified
June 16, 2009.
Version 130.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Transcription factor AP-1 Alternative name(s): Activator protein 1 Short name=AP1 Proto-oncogene c-jun V-jun avian sarcoma virus 17 oncogene homolog p39 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 331 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. |
| Subunit structure | Heterodimer with either FOS or BATF3. Interacts with HIVEP3 By similarity. Interacts with SMAD3/SMAD4 heterodimers. Interacts with MYBBP1A, SPIB and TCF20. Interacts with COPS5; indirectly leading to its phosphorylation. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. |
| Subcellular location | |
| Post-translational modification | Phosphorylation enhances the transcriptional activity. Phosphorylated by PRKDC. Ref.7 Ref.13 Ref.14 |
| Sequence similarities | Belongs to the bZIP family. Jun subfamily. Contains 1 bZIP domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| EDF1 | O60869-1 | 1 | EBI-852823,EBI-781310 | |
| jnk-1 | Q8WQG9 | 2 | EBI-852823,EBI-321822 | From a different organism. |
| MAPK8 | P45983 | 2 | EBI-852823,EBI-286483 | |
| MAPK8 | P45983-1 | 2 | EBI-852823,EBI-288687 | |
| MAPK9 | P45984-1 | 1 | EBI-852823,EBI-713586 | |
| VRK1 | Q99986 | 3 | EBI-852823,EBI-1769146 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 331 | 331 | Transcription factor AP-1 | PRO_0000076429 | |||||||
Regions | |||||||||||
| Domain | 280 – 308 | 29 | Leucine-zipper | ||||||||
| DNA binding | 257 – 276 | 20 | Basic motif | ||||||||
Amino acid modifications | |||||||||||
| Modified residue | 58 | 1 | Phosphoserine Ref.14 | ||||||||
| Modified residue | 63 | 1 | Phosphoserine; by MAPK8 Ref.14 | ||||||||
| Modified residue | 73 | 1 | Phosphoserine; by MAPK8 Ref.13 Ref.14 | ||||||||
| Modified residue | 239 | 1 | Phosphothreonine Ref.14 | ||||||||
| Modified residue | 243 | 1 | Phosphoserine Ref.14 | ||||||||
Natural variations | |||||||||||
| Natural variant | 297 | 1 | T → M: dbSNP rs9989. | VAR_012070 | |||||||
Experimental info | |||||||||||
| Sequence conflict | 11 | 1 | D → G AA sequence Ref.2 | ||||||||
| Sequence conflict | 14 | 1 | L → F AA sequence Ref.2 | ||||||||
| Sequence conflict | 80 | 1 | I → V AA sequence Ref.2 | ||||||||
Secondary structure | |||||||||||
Helix Strand Turn | |||||||||||
| Helix | 255 – 306 | 52 | |||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure and chromosomal localization of the functional intronless human JUN protooncogene." Hattori K., Angel P., le Beau M.M., Karin M. Proc. Natl. Acad. Sci. U.S.A. 85:9148-9152(1988) [PubMed: 3194415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1." Bohmann D., Bos T.J., Admon A., Nishimura T., Vogt P.K., Tjian R. Science 238:1386-1392(1987) [PubMed: 2825349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | NIEHS SNPs program Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [5] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: B-cell, Ovary, Testis and Uterus. |
| [7] | "c-Jun is phosphorylated by the DNA-dependent protein kinase in vitro; definition of the minimal kinase recognition motif." Bannister A.J., Gottlieb T.M., Kouzarides T., Jackson S.P. Nucleic Acids Res. 21:1289-1295(1993) [PubMed: 8464713] [Abstract] Cited for: PHOSPHORYLATION. |
| [8] | "Cross-talk between different enhancer elements during mitogenic induction of the human stromelysin-1 gene." Kirstein M., Sanz L., Moscat J., Diaz-Meco M.T., Saus J. J. Biol. Chem. 271:18231-18236(1996) [PubMed: 8663478] [Abstract] Cited for: INTERACTION WITH TCF20. |
| [9] | "A new group of conserved coactivators that increase the specificity of AP-1 transcription factors." Claret F.-X., Hibi M., Dhut S., Toda T., Karin M. Nature 383:453-457(1996) [PubMed: 8837781] [Abstract] Cited for: INTERACTION WITH COPS5. |
| [10] | "SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1." Rao S., Matsumura A., Yoon J., Simon M.C. J. Biol. Chem. 274:11115-11124(1999) [PubMed: 10196196] [Abstract] Cited for: INTERACTION WITH SPIB. |
| [11] | "Correlation of transcriptional repression by p21(SNFT) with changes in DNA.NF-AT complex interactions." Bower K.E., Zeller R.W., Wachsman W., Martinez T., McGuire K.L. J. Biol. Chem. 277:34967-34977(2002) [PubMed: 12087103] [Abstract] Cited for: INTERACTION WITH BATF3. |
| [12] | "Transcriptional repression of MMP-1 by p21SNFT and reduced in vitro invasiveness of hepatocarcinoma cells." Bower K.E., Fritz J.M., McGuire K.L. Oncogene 23:8805-8814(2004) [PubMed: 15467742] [Abstract] Cited for: INTERACTION WITH BATF3. |
| [13] | "Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, MASS SPECTROMETRY. Tissue: Epithelium. |
| [14] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58; SER-63; SER-73; THR-239 AND SER-243, MASS SPECTROMETRY. |
| [15] | "Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA." Glover J.N., Harrison S.C. Nature 373:257-261(1995) [PubMed: 7816143] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF 257-313 OF COMPLEX WITH FOS. |
| [16] | "High resolution NMR solution structure of the leucine zipper domain of the c-Jun homodimer." Junius F.K., O'Donoghue S.I., Nilges M., Weiss A.S., King G.F. J. Biol. Chem. 271:13663-13667(1996) [PubMed: 8662824] [Abstract] Cited for: STRUCTURE BY NMR OF 276-314. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| J04111 Genomic DNA. Translation: AAA59197.1. BT019759 mRNA. Translation: AAV38564.1. AY217548 Genomic DNA. Translation: AAO22993.1. AL136985 Genomic DNA. Translation: CAC10201.1. BC002646 mRNA. No translation available. BC006175 mRNA. Translation: AAH06175.1. BC009874 mRNA. Translation: AAH09874.2. BC068522 mRNA. Translation: AAH68522.1. | |||||||||||||||||||||||||||||||||||||||||||
| IPI | IPI00008965. | ||||||||||||||||||||||||||||||||||||||||||
| PIR | TVHUJN. A31264. | ||||||||||||||||||||||||||||||||||||||||||
| RefSeq | NP_002219.1. | ||||||||||||||||||||||||||||||||||||||||||
| UniGene | Hs.714791 | ||||||||||||||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||||||||||||||
| DIP | DIP:1054N. DIP:5961N. | ||||||||||||||||||||||||||||||||||||||||||
| IntAct | P05412. 9 interactions. | ||||||||||||||||||||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||||||||||||||||||||
| PhosphoSite | P05412. | ||||||||||||||||||||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||||||||||||||||||||
| PRIDE | P05412. | ||||||||||||||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||||||||||||||
| Ensembl | ENSG00000177606. Homo sapiens. [Contig view] | ||||||||||||||||||||||||||||||||||||||||||
| GeneID | 3725. | ||||||||||||||||||||||||||||||||||||||||||
| KEGG | hsa:3725. | ||||||||||||||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||||||||||||||
| GeneCards | GC01M058958. | ||||||||||||||||||||||||||||||||||||||||||
| H-InvDB | HIX0000635. | ||||||||||||||||||||||||||||||||||||||||||
| HGNC | HGNC:6204. JUN. | ||||||||||||||||||||||||||||||||||||||||||
| HPA | CAB003801. CAB007780. | ||||||||||||||||||||||||||||||||||||||||||
| MIM | 165160. gene. | ||||||||||||||||||||||||||||||||||||||||||
| PharmGKB | PA30006. | ||||||||||||||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||||||||||||||
| HOGENOM | P05412. | ||||||||||||||||||||||||||||||||||||||||||
| HOVERGEN | P05412. | ||||||||||||||||||||||||||||||||||||||||||
| OMA | P05412. KPHLRNK. | ||||||||||||||||||||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||||||||||||||||||||
| Pathway_Interaction_DB | bcr_5pathway. BCR signaling pathway. nfat_tfpathway. Calcineurin-regulated NFAT-dependent transcription in lymphocytes. tcrcalciumpathway. Calcium signaling in the CD4+ TCR pathway. cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells. endothelinpathway. Endothelins. fcer1pathway. Fc-epsilon receptor I signaling in mast cells. fgf_pathway. FGF signaling pathway. hnf3apathway. FOXA1 transcription factor network. hif1_tfpathway. HIF-1-alpha transcription factor network. il1pathway. IL1-mediated signaling events. il12_stat4pathway. IL12 signaling mediated by STAT4. il2_1pathway. IL2-mediated signaling events. il6_7pathway. IL6-mediated signaling events. tcrjnkpathway. JNK signaling in the CD4+ TCR pathway. lysophospholipid_pathway. LPA receptor mediated events. avb3_opn_pathway. Osteopontin-mediated events. pdgfrapathway. PDGFR-alpha signaling pathway. ps1pathway. Presenilin action in Notch and Wnt signaling. ar_tf_pathway. Regulation of Androgen receptor activity. smad2_3nuclearpathway. Regulation of nuclear SMAD2/3 signaling. telomerasepathway. Regulation of Telomerase. s1p_s1p2_pathway. S1P2 pathway. met_pathway. Signaling events activated by Hepatocyte Growth Factor Receptor (c-Met). ret_pathway. Signaling events regulated by Ret tyrosine kinase. p38alphabetadownstreampathway. Signaling mediated by p38-alpha and p38-beta. | ||||||||||||||||||||||||||||||||||||||||||
| Reactome | REACT_6900. Signaling in Immune system. | ||||||||||||||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||||||||||||||
| ArrayExpress | P05412. | ||||||||||||||||||||||||||||||||||||||||||
| Bgee | P05412. | ||||||||||||||||||||||||||||||||||||||||||
| CleanEx | HS_JUN. | ||||||||||||||||||||||||||||||||||||||||||
| GermOnline | ENSG00000177606. Homo sapiens. | ||||||||||||||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||||||||||||||
| InterPro | IPR011616. bZIP_1. IPR015558. C_Jun. IPR005643. JNK. IPR002112. Leuzip_Jun. IPR004827. TF_bZIP. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| PANTHER | PTHR11462:SF8. C_Jun. 1 hit. | ||||||||||||||||||||||||||||||||||||||||||
| Pfam | PF00170. bZIP_1. 1 hit. PF03957. Jun. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| PRINTS | PR00043. LEUZIPPRJUN. | ||||||||||||||||||||||||||||||||||||||||||
| SMART | SM00338. BRLZ. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| PROSITE | PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||||||||||||||
| DrugBank | DB01169. Arsenic trioxide. DB01029. Irbesartan. DB00570. Vinblastine. | ||||||||||||||||||||||||||||||||||||||||||
| NextBio | 14583. | ||||||||||||||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||||||||||||||
Entry information
| Entry name | JUN_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P05412 Secondary accession number(s): Q96G93 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


