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P05408

- 7B2_HUMAN

UniProt

P05408 - 7B2_HUMAN

Protein

Neuroendocrine protein 7B2

Gene

SCG5

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (10 Oct 2002)
      Previous versions | rss
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    Functioni

    Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro.1 Publication

    GO - Molecular functioni

    1. enzyme inhibitor activity Source: UniProtKB
    2. GTP binding Source: ProtInc
    3. protein binding Source: UniProtKB
    4. unfolded protein binding Source: UniProtKB

    GO - Biological processi

    1. intracellular protein transport Source: UniProtKB
    2. neuropeptide signaling pathway Source: UniProtKB-KW
    3. peptide hormone processing Source: UniProtKB
    4. regulation of hormone secretion Source: UniProtKB

    Keywords - Molecular functioni

    Chaperone, Neuropeptide

    Keywords - Biological processi

    Transport

    Protein family/group databases

    MEROPSiI21.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuroendocrine protein 7B2
    Alternative name(s):
    Pituitary polypeptide
    Secretogranin V
    Secretogranin-5
    Secretory granule endocrine protein I
    Cleaved into the following 2 chains:
    Gene namesi
    Name:SCG5
    Synonyms:SGNE1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:10816. SCG5.

    Subcellular locationi

    Secreted
    Note: Neuroendocrine and endocrine secretory granules.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. secretory granule Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35724.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 26261 PublicationAdd
    BLAST
    Chaini27 – 212186Neuroendocrine protein 7B2PRO_0000000041Add
    BLAST
    Chaini27 – 176150N-terminal peptideBy similarityPRO_0000000042Add
    BLAST
    Peptidei200 – 21213C-terminal peptideBy similarityPRO_0000000043Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi120 ↔ 130By similarity

    Post-translational modificationi

    Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides.By similarity
    Sulfated on tyrosine residues.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Sulfation

    Proteomic databases

    PaxDbiP05408.
    PRIDEiP05408.

    PTM databases

    PhosphoSiteiP05408.

    Expressioni

    Gene expression databases

    ArrayExpressiP05408.
    BgeeiP05408.
    CleanExiHS_SCG5.
    GenevestigatoriP05408.

    Organism-specific databases

    HPAiHPA013136.

    Interactioni

    Subunit structurei

    Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages.1 Publication

    Protein-protein interaction databases

    BioGridi112345. 3 interactions.
    IntActiP05408. 2 interactions.
    MINTiMINT-1402757.
    STRINGi9606.ENSP00000300175.

    Structurei

    3D structure databases

    ProteinModelPortaliP05408.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 7B2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG271457.
    HOGENOMiHOG000259690.
    HOVERGENiHBG000031.
    InParanoidiP05408.
    OMAiNNGISIS.
    OrthoDBiEOG7KSX9R.
    PhylomeDBiP05408.
    TreeFamiTF314328.

    Family and domain databases

    InterProiIPR007945. Secretogranin_V.
    [Graphical view]
    PANTHERiPTHR12738. PTHR12738. 1 hit.
    PfamiPF05281. Secretogranin_V. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P05408-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVSRMVSTML SGLLFWLASG WTPAFAYSPR TPDRVSEADI QRLLHGVMEQ    50
    LGIARPRVEY PAHQAMNLVG PQSIEGGAHE GLQHLGPFGN IPNIVAELTG 100
    DNIPKDFSED QGYPDPPNPC PVGKTADDGC LENTPDTAEF SREFQLHQHL 150
    FDPEHDYPGL GKWNKKLLYE KMKGGERRKR RSVNPYLQGQ RLDNVVAKKS 200
    VPHFSDEDKD PE 212
    Length:212
    Mass (Da):23,730
    Last modified:October 10, 2002 - v2
    Checksum:iA459702055C0FE84
    GO
    Isoform 2 (identifier: P05408-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         126-126: Missing.

    Show »
    Length:211
    Mass (Da):23,659
    Checksum:i649AA3FF2EC2A0A6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti151 – 1511F → S in AAH05349. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei126 – 1261Missing in isoform 2. 4 PublicationsVSP_011754

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00757 mRNA. Translation: CAA68726.1.
    AJ290438
    , AJ290439, AJ290440, AJ290441, AJ290442 Genomic DNA. Translation: CAB90397.1.
    CR541824 mRNA. Translation: CAG46623.1.
    CH471125 Genomic DNA. Translation: EAW92262.1.
    BC005349 mRNA. Translation: AAH05349.1.
    BC093053 mRNA. Translation: AAH93053.1.
    CCDSiCCDS45207.1. [P05408-1]
    CCDS45208.1. [P05408-2]
    PIRiS01008. PUHU.
    RefSeqiNP_001138229.1. NM_001144757.1. [P05408-1]
    NP_003011.1. NM_003020.3. [P05408-2]
    UniGeneiHs.156540.

    Genome annotation databases

    EnsembliENST00000300175; ENSP00000300175; ENSG00000166922. [P05408-1]
    ENST00000413748; ENSP00000388560; ENSG00000166922. [P05408-2]
    GeneIDi6447.
    KEGGihsa:6447.
    UCSCiuc001zgz.2. human.
    uc001zha.2. human. [P05408-1]

    Polymorphism databases

    DMDMi23830842.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00757 mRNA. Translation: CAA68726.1 .
    AJ290438
    , AJ290439 , AJ290440 , AJ290441 , AJ290442 Genomic DNA. Translation: CAB90397.1 .
    CR541824 mRNA. Translation: CAG46623.1 .
    CH471125 Genomic DNA. Translation: EAW92262.1 .
    BC005349 mRNA. Translation: AAH05349.1 .
    BC093053 mRNA. Translation: AAH93053.1 .
    CCDSi CCDS45207.1. [P05408-1 ]
    CCDS45208.1. [P05408-2 ]
    PIRi S01008. PUHU.
    RefSeqi NP_001138229.1. NM_001144757.1. [P05408-1 ]
    NP_003011.1. NM_003020.3. [P05408-2 ]
    UniGenei Hs.156540.

    3D structure databases

    ProteinModelPortali P05408.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112345. 3 interactions.
    IntActi P05408. 2 interactions.
    MINTi MINT-1402757.
    STRINGi 9606.ENSP00000300175.

    Protein family/group databases

    MEROPSi I21.001.

    PTM databases

    PhosphoSitei P05408.

    Polymorphism databases

    DMDMi 23830842.

    Proteomic databases

    PaxDbi P05408.
    PRIDEi P05408.

    Protocols and materials databases

    DNASUi 6447.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000300175 ; ENSP00000300175 ; ENSG00000166922 . [P05408-1 ]
    ENST00000413748 ; ENSP00000388560 ; ENSG00000166922 . [P05408-2 ]
    GeneIDi 6447.
    KEGGi hsa:6447.
    UCSCi uc001zgz.2. human.
    uc001zha.2. human. [P05408-1 ]

    Organism-specific databases

    CTDi 6447.
    GeneCardsi GC15P032933.
    HGNCi HGNC:10816. SCG5.
    HPAi HPA013136.
    MIMi 173120. gene.
    neXtProti NX_P05408.
    PharmGKBi PA35724.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG271457.
    HOGENOMi HOG000259690.
    HOVERGENi HBG000031.
    InParanoidi P05408.
    OMAi NNGISIS.
    OrthoDBi EOG7KSX9R.
    PhylomeDBi P05408.
    TreeFami TF314328.

    Miscellaneous databases

    GeneWikii SCG5.
    GenomeRNAii 6447.
    NextBioi 25057.
    PROi P05408.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P05408.
    Bgeei P05408.
    CleanExi HS_SCG5.
    Genevestigatori P05408.

    Family and domain databases

    InterProi IPR007945. Secretogranin_V.
    [Graphical view ]
    PANTHERi PTHR12738. PTHR12738. 1 hit.
    Pfami PF05281. Secretogranin_V. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequence analysis of human pituitary cDNA encoding the novel polypeptide 7B2."
      Martens G.J.M.
      FEBS Lett. 234:160-164(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Pituitary.
    2. "The production by alternate splicing of two mRNAs differing by one codon could be an intrinsic property of neuroendocrine protein 7B2 gene expression in man."
      Paquet L., Lazure C., Seidah N.G., Chretien M., Mbikay M.
      Biochem. Biophys. Res. Commun. 174:156-162(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 125-140 (ISOFORM 1), PROTEIN SEQUENCE OF 126-132 (ISOFORM 2).
      Tissue: Pituitary.
    3. "Human neuroendocrine protein 7B2 genomic organization."
      Jackson R.S.
      Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Pancreas.
    7. "Structural organization of the gene encoding the neuroendocrine chaperone 7B2."
      Braks J.A., Broers C.A., Danger J.M., Martens G.J.M.
      Eur. J. Biochem. 236:60-67(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-92.
    8. "Isolation and NH2-terminal sequence of a highly conserved human and porcine pituitary protein belonging to a new superfamily. Immunocytochemical localization in pars distalis and pars nervosa of the pituitary and in the supraoptic nucleus of the hypothalamus."
      Seidah N.G., Hsi K.L., de Serres G., Rochemont J., Hamelin J., Antakly T., Cantin M., Chretien M.
      Arch. Biochem. Biophys. 225:525-534(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 27-103.
    9. "7B2 is a neuroendocrine chaperone that transiently interacts with prohormone convertase PC2 in the secretory pathway."
      Braks J.A., Martens G.J.M.
      Cell 78:263-273(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PCSK2.
    10. "Neuroendocrine secretory protein 7B2: structure, expression and functions."
      Mbikay M., Seidah N.G., Chretien M.
      Biochem. J. 357:329-342(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry namei7B2_HUMAN
    AccessioniPrimary (citable) accession number: P05408
    Secondary accession number(s): P01164, Q6FHD0, Q9BS38
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 10, 2002
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3