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Protein

Neuroendocrine protein 7B2

Gene

SCG5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro.1 Publication

GO - Molecular functioni

  1. enzyme inhibitor activity Source: UniProtKB
  2. GTP binding Source: ProtInc
  3. unfolded protein binding Source: UniProtKB

GO - Biological processi

  1. intracellular protein transport Source: UniProtKB
  2. neuropeptide signaling pathway Source: UniProtKB-KW
  3. peptide hormone processing Source: UniProtKB
  4. regulation of hormone secretion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Neuropeptide

Keywords - Biological processi

Transport

Protein family/group databases

MEROPSiI21.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine protein 7B2
Alternative name(s):
Pituitary polypeptide
Secretogranin V
Secretogranin-5
Secretory granule endocrine protein I
Cleaved into the following 2 chains:
Gene namesi
Name:SCG5
Synonyms:SGNE1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componentsi: Chromosome 15, Unplaced

Organism-specific databases

HGNCiHGNC:10816. SCG5.

Subcellular locationi

  1. Secreted

  2. Note: Neuroendocrine and endocrine secretory granules.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. secretory granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35724.

Polymorphism and mutation databases

BioMutaiSCG5.
DMDMi23830842.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 212186Neuroendocrine protein 7B2PRO_0000000041Add
BLAST
Chaini27 – 176150N-terminal peptideBy similarityPRO_0000000042Add
BLAST
Peptidei200 – 21213C-terminal peptideBy similarityPRO_0000000043Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi120 ↔ 130By similarity

Post-translational modificationi

Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides.By similarity
Sulfated on tyrosine residues.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Sulfation

Proteomic databases

PaxDbiP05408.
PRIDEiP05408.

PTM databases

PhosphoSiteiP05408.

Expressioni

Gene expression databases

BgeeiP05408.
CleanExiHS_SCG5.
ExpressionAtlasiP05408. baseline and differential.
GenevestigatoriP05408.

Organism-specific databases

HPAiHPA013136.

Interactioni

Subunit structurei

Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
UBQLN1Q9UMX03EBI-722635,EBI-741480
UBQLN1Q9UMX0-23EBI-722635,EBI-10173939

Protein-protein interaction databases

BioGridi112345. 4 interactions.
IntActiP05408. 3 interactions.
MINTiMINT-1402757.
STRINGi9606.ENSP00000300175.

Structurei

3D structure databases

ProteinModelPortaliP05408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 7B2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG271457.
GeneTreeiENSGT00390000009816.
HOGENOMiHOG000259690.
HOVERGENiHBG000031.
InParanoidiP05408.
OMAiQATNIVG.
OrthoDBiEOG7KSX9R.
PhylomeDBiP05408.
TreeFamiTF314328.

Family and domain databases

InterProiIPR007945. Secretogranin_V.
[Graphical view]
PANTHERiPTHR12738. PTHR12738. 1 hit.
PfamiPF05281. Secretogranin_V. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P05408-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSRMVSTML SGLLFWLASG WTPAFAYSPR TPDRVSEADI QRLLHGVMEQ
60 70 80 90 100
LGIARPRVEY PAHQAMNLVG PQSIEGGAHE GLQHLGPFGN IPNIVAELTG
110 120 130 140 150
DNIPKDFSED QGYPDPPNPC PVGKTADDGC LENTPDTAEF SREFQLHQHL
160 170 180 190 200
FDPEHDYPGL GKWNKKLLYE KMKGGERRKR RSVNPYLQGQ RLDNVVAKKS
210
VPHFSDEDKD PE
Length:212
Mass (Da):23,730
Last modified:October 10, 2002 - v2
Checksum:iA459702055C0FE84
GO
Isoform 2 (identifier: P05408-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     126-126: Missing.

Show »
Length:211
Mass (Da):23,659
Checksum:i649AA3FF2EC2A0A6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511F → S in AAH05349 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei126 – 1261Missing in isoform 2. 4 PublicationsVSP_011754

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00757 mRNA. Translation: CAA68726.1.
AJ290438
, AJ290439, AJ290440, AJ290441, AJ290442 Genomic DNA. Translation: CAB90397.1.
CR541824 mRNA. Translation: CAG46623.1.
CH471125 Genomic DNA. Translation: EAW92262.1.
BC005349 mRNA. Translation: AAH05349.1.
BC093053 mRNA. Translation: AAH93053.1.
CCDSiCCDS45207.1. [P05408-1]
CCDS45208.1. [P05408-2]
PIRiS01008. PUHU.
RefSeqiNP_001138229.1. NM_001144757.1. [P05408-1]
NP_003011.1. NM_003020.3. [P05408-2]
UniGeneiHs.156540.

Genome annotation databases

EnsembliENST00000300175; ENSP00000300175; ENSG00000166922. [P05408-1]
ENST00000413748; ENSP00000388560; ENSG00000166922. [P05408-2]
ENST00000614359; ENSP00000482615; ENSG00000277614. [P05408-1]
GeneIDi6447.
KEGGihsa:6447.
UCSCiuc001zgz.2. human.
uc001zha.2. human. [P05408-1]

Polymorphism and mutation databases

BioMutaiSCG5.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00757 mRNA. Translation: CAA68726.1.
AJ290438
, AJ290439, AJ290440, AJ290441, AJ290442 Genomic DNA. Translation: CAB90397.1.
CR541824 mRNA. Translation: CAG46623.1.
CH471125 Genomic DNA. Translation: EAW92262.1.
BC005349 mRNA. Translation: AAH05349.1.
BC093053 mRNA. Translation: AAH93053.1.
CCDSiCCDS45207.1. [P05408-1]
CCDS45208.1. [P05408-2]
PIRiS01008. PUHU.
RefSeqiNP_001138229.1. NM_001144757.1. [P05408-1]
NP_003011.1. NM_003020.3. [P05408-2]
UniGeneiHs.156540.

3D structure databases

ProteinModelPortaliP05408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112345. 4 interactions.
IntActiP05408. 3 interactions.
MINTiMINT-1402757.
STRINGi9606.ENSP00000300175.

Protein family/group databases

MEROPSiI21.001.

PTM databases

PhosphoSiteiP05408.

Polymorphism and mutation databases

BioMutaiSCG5.
DMDMi23830842.

Proteomic databases

PaxDbiP05408.
PRIDEiP05408.

Protocols and materials databases

DNASUi6447.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300175; ENSP00000300175; ENSG00000166922. [P05408-1]
ENST00000413748; ENSP00000388560; ENSG00000166922. [P05408-2]
ENST00000614359; ENSP00000482615; ENSG00000277614. [P05408-1]
GeneIDi6447.
KEGGihsa:6447.
UCSCiuc001zgz.2. human.
uc001zha.2. human. [P05408-1]

Organism-specific databases

CTDi6447.
GeneCardsiGC15P032933.
HGNCiHGNC:10816. SCG5.
HPAiHPA013136.
MIMi173120. gene.
neXtProtiNX_P05408.
PharmGKBiPA35724.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG271457.
GeneTreeiENSGT00390000009816.
HOGENOMiHOG000259690.
HOVERGENiHBG000031.
InParanoidiP05408.
OMAiQATNIVG.
OrthoDBiEOG7KSX9R.
PhylomeDBiP05408.
TreeFamiTF314328.

Miscellaneous databases

ChiTaRSiSCG5. human.
GeneWikiiSCG5.
GenomeRNAii6447.
NextBioi25057.
PROiP05408.
SOURCEiSearch...

Gene expression databases

BgeeiP05408.
CleanExiHS_SCG5.
ExpressionAtlasiP05408. baseline and differential.
GenevestigatoriP05408.

Family and domain databases

InterProiIPR007945. Secretogranin_V.
[Graphical view]
PANTHERiPTHR12738. PTHR12738. 1 hit.
PfamiPF05281. Secretogranin_V. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of human pituitary cDNA encoding the novel polypeptide 7B2."
    Martens G.J.M.
    FEBS Lett. 234:160-164(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Pituitary.
  2. "The production by alternate splicing of two mRNAs differing by one codon could be an intrinsic property of neuroendocrine protein 7B2 gene expression in man."
    Paquet L., Lazure C., Seidah N.G., Chretien M., Mbikay M.
    Biochem. Biophys. Res. Commun. 174:156-162(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 125-140 (ISOFORM 1), PROTEIN SEQUENCE OF 126-132 (ISOFORM 2).
    Tissue: Pituitary.
  3. "Human neuroendocrine protein 7B2 genomic organization."
    Jackson R.S.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Pancreas.
  7. "Structural organization of the gene encoding the neuroendocrine chaperone 7B2."
    Braks J.A., Broers C.A., Danger J.M., Martens G.J.M.
    Eur. J. Biochem. 236:60-67(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-92.
  8. "Isolation and NH2-terminal sequence of a highly conserved human and porcine pituitary protein belonging to a new superfamily. Immunocytochemical localization in pars distalis and pars nervosa of the pituitary and in the supraoptic nucleus of the hypothalamus."
    Seidah N.G., Hsi K.L., de Serres G., Rochemont J., Hamelin J., Antakly T., Cantin M., Chretien M.
    Arch. Biochem. Biophys. 225:525-534(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-103.
  9. "7B2 is a neuroendocrine chaperone that transiently interacts with prohormone convertase PC2 in the secretory pathway."
    Braks J.A., Martens G.J.M.
    Cell 78:263-273(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PCSK2.
  10. "Neuroendocrine secretory protein 7B2: structure, expression and functions."
    Mbikay M., Seidah N.G., Chretien M.
    Biochem. J. 357:329-342(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry namei7B2_HUMAN
AccessioniPrimary (citable) accession number: P05408
Secondary accession number(s): P01164, Q6FHD0, Q9BS38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 10, 2002
Last modified: April 29, 2015
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.