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P05408 (7B2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuroendocrine protein 7B2
Alternative name(s):
Pituitary polypeptide
Secretogranin V
Secretogranin-5
Secretory granule endocrine protein I

Cleaved into the following 2 chains:

  1. N-terminal peptide
  2. C-terminal peptide
Gene names
Name:SCG5
Synonyms:SGNE1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro. Ref.9

Subunit structure

Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages. Ref.9

Subcellular location

Secreted. Note: Neuroendocrine and endocrine secretory granules.

Post-translational modification

Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides By similarity.

Sulfated on tyrosine residues By similarity.

Sequence similarities

Belongs to the 7B2 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P05408-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P05408-2)

The sequence of this isoform differs from the canonical sequence as follows:
     126-126: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Ref.8
Chain27 – 212186Neuroendocrine protein 7B2
PRO_0000000041
Chain27 – 176150N-terminal peptide By similarity
PRO_0000000042
Peptide200 – 21213C-terminal peptide By similarity
PRO_0000000043

Amino acid modifications

Disulfide bond120 ↔ 130 By similarity

Natural variations

Alternative sequence1261Missing in isoform 2.
VSP_011754

Experimental info

Sequence conflict1511F → S in AAH05349. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 10, 2002. Version 2.
Checksum: A459702055C0FE84

FASTA21223,730
        10         20         30         40         50         60 
MVSRMVSTML SGLLFWLASG WTPAFAYSPR TPDRVSEADI QRLLHGVMEQ LGIARPRVEY 

        70         80         90        100        110        120 
PAHQAMNLVG PQSIEGGAHE GLQHLGPFGN IPNIVAELTG DNIPKDFSED QGYPDPPNPC 

       130        140        150        160        170        180 
PVGKTADDGC LENTPDTAEF SREFQLHQHL FDPEHDYPGL GKWNKKLLYE KMKGGERRKR 

       190        200        210 
RSVNPYLQGQ RLDNVVAKKS VPHFSDEDKD PE 

« Hide

Isoform 2 [UniParc].

Checksum: 649AA3FF2EC2A0A6
Show »

FASTA21123,659

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of human pituitary cDNA encoding the novel polypeptide 7B2."
Martens G.J.M.
FEBS Lett. 234:160-164(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Pituitary.
[2]"The production by alternate splicing of two mRNAs differing by one codon could be an intrinsic property of neuroendocrine protein 7B2 gene expression in man."
Paquet L., Lazure C., Seidah N.G., Chretien M., Mbikay M.
Biochem. Biophys. Res. Commun. 174:156-162(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 125-140 (ISOFORM 1), PROTEIN SEQUENCE OF 126-132 (ISOFORM 2).
Tissue: Pituitary.
[3]"Human neuroendocrine protein 7B2 genomic organization."
Jackson R.S.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Pancreas.
[7]"Structural organization of the gene encoding the neuroendocrine chaperone 7B2."
Braks J.A., Broers C.A., Danger J.M., Martens G.J.M.
Eur. J. Biochem. 236:60-67(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-92.
[8]"Isolation and NH2-terminal sequence of a highly conserved human and porcine pituitary protein belonging to a new superfamily. Immunocytochemical localization in pars distalis and pars nervosa of the pituitary and in the supraoptic nucleus of the hypothalamus."
Seidah N.G., Hsi K.L., de Serres G., Rochemont J., Hamelin J., Antakly T., Cantin M., Chretien M.
Arch. Biochem. Biophys. 225:525-534(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 27-103.
[9]"7B2 is a neuroendocrine chaperone that transiently interacts with prohormone convertase PC2 in the secretory pathway."
Braks J.A., Martens G.J.M.
Cell 78:263-273(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PCSK2.
[10]"Neuroendocrine secretory protein 7B2: structure, expression and functions."
Mbikay M., Seidah N.G., Chretien M.
Biochem. J. 357:329-342(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00757 mRNA. Translation: CAA68726.1.
AJ290438 expand/collapse EMBL AC list , AJ290439, AJ290440, AJ290441, AJ290442 Genomic DNA. Translation: CAB90397.1.
CR541824 mRNA. Translation: CAG46623.1.
CH471125 Genomic DNA. Translation: EAW92262.1.
BC005349 mRNA. Translation: AAH05349.1.
BC093053 mRNA. Translation: AAH93053.1.
CCDSCCDS45207.1. [P05408-1]
CCDS45208.1. [P05408-2]
PIRPUHU. S01008.
RefSeqNP_001138229.1. NM_001144757.1. [P05408-1]
NP_003011.1. NM_003020.3. [P05408-2]
UniGeneHs.156540.

3D structure databases

ProteinModelPortalP05408.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112345. 3 interactions.
IntActP05408. 2 interactions.
MINTMINT-1402757.
STRING9606.ENSP00000300175.

Protein family/group databases

MEROPSI21.001.

PTM databases

PhosphoSiteP05408.

Polymorphism databases

DMDM23830842.

Proteomic databases

PaxDbP05408.
PRIDEP05408.

Protocols and materials databases

DNASU6447.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000300175; ENSP00000300175; ENSG00000166922. [P05408-1]
ENST00000413748; ENSP00000388560; ENSG00000166922. [P05408-2]
GeneID6447.
KEGGhsa:6447.
UCSCuc001zgz.2. human.
uc001zha.2. human. [P05408-1]

Organism-specific databases

CTD6447.
GeneCardsGC15P032933.
HGNCHGNC:10816. SCG5.
HPAHPA013136.
MIM173120. gene.
neXtProtNX_P05408.
PharmGKBPA35724.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG271457.
HOGENOMHOG000259690.
HOVERGENHBG000031.
InParanoidP05408.
OMANNGISIS.
OrthoDBEOG7KSX9R.
PhylomeDBP05408.
TreeFamTF314328.

Gene expression databases

ArrayExpressP05408.
BgeeP05408.
CleanExHS_SCG5.
GenevestigatorP05408.

Family and domain databases

InterProIPR007945. Secretogranin_V.
[Graphical view]
PANTHERPTHR12738. PTHR12738. 1 hit.
PfamPF05281. Secretogranin_V. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiSCG5.
GenomeRNAi6447.
NextBio25057.
PROP05408.
SOURCESearch...

Entry information

Entry name7B2_HUMAN
AccessionPrimary (citable) accession number: P05408
Secondary accession number(s): P01164, Q6FHD0, Q9BS38
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 10, 2002
Last modified: July 9, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM