P05408 (7B2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuroendocrine protein 7B2

Alternative name(s):
Pituitary polypeptide
Secretogranin V
Secretogranin-5
Secretory granule endocrine protein I

Cleaved into the following 2 chains:

1. N-terminal peptide
2. C-terminal peptide

Gene names

Name:	SCG5
Synonyms:	SGNE1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro. Ref.7
Subunit structure	Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages. Ref.7
Subcellular location	Secreted. Note: Neuroendocrine and endocrine secretory granules.
Post-translational modification	Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides By similarity. Sulfated on tyrosine residues By similarity.
Sequence similarities	Belongs to the 7B2 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Chaperone Neuropeptide
PTM	Cleavage on pair of basic residues Disulfide bond Sulfation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from sequence or structural similarity. Source: UniProtKB neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW peptide hormone processing Inferred from sequence or structural similarity. Source: UniProtKB regulation of hormone secretion Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell secretory granule Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	GTP binding Traceable author statement Ref.1. Source: ProtInc enzyme inhibitor activity Inferred from sequence or structural similarity. Source: UniProtKB unfolded protein binding Inferred from direct assay Ref.7. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P05408-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P05408-2)

The sequence of this isoform differs from the canonical sequence as follows:
     126-126: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.6
	Chain	27 – 212	186	Neuroendocrine protein 7B2		PRO_0000000041
	Chain	27 – 176	150	N-terminal peptide By similarity		PRO_0000000042
	Peptide	200 – 212	13	C-terminal peptide By similarity		PRO_0000000043
Amino acid modifications
	Disulfide bond	120 ↔ 130		By similarity
Natural variations
	Alternative sequence	126	1	Missing in isoform 2.		VSP_011754
Experimental info
	Sequence conflict	151	1	F → S in AAH05349. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 10, 2002. Version 2. Checksum: A459702055C0FE84	FASTA	212	23,730
	10 20 30 40 50 60 MVSRMVSTML SGLLFWLASG WTPAFAYSPR TPDRVSEADI QRLLHGVMEQ LGIARPRVEY 70 80 90 100 110 120 PAHQAMNLVG PQSIEGGAHE GLQHLGPFGN IPNIVAELTG DNIPKDFSED QGYPDPPNPC 130 140 150 160 170 180 PVGKTADDGC LENTPDTAEF SREFQLHQHL FDPEHDYPGL GKWNKKLLYE KMKGGERRKR 190 200 210 RSVNPYLQGQ RLDNVVAKKS VPHFSDEDKD PE
	Isoform 2 [UniParc]. Checksum: 649AA3FF2EC2A0A6	FASTA	211	23,659
	10 20 30 40 50 60 MVSRMVSTML SGLLFWLASG WTPAFAYSPR TPDRVSEADI QRLLHGVMEQ LGIARPRVEY 70 80 90 100 110 120 PAHQAMNLVG PQSIEGGAHE GLQHLGPFGN IPNIVAELTG DNIPKDFSED QGYPDPPNPC 130 140 150 160 170 180 PVGKTDDGCL ENTPDTAEFS REFQLHQHLF DPEHDYPGLG KWNKKLLYEK MKGGERRKRR 190 200 210 SVNPYLQGQR LDNVVAKKSV PHFSDEDKDP E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of human pituitary cDNA encoding the novel polypeptide 7B2." Martens G.J.M. FEBS Lett. 234:160-164(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Pituitary.
[2]	"The production by alternate splicing of two mRNAs differing by one codon could be an intrinsic property of neuroendocrine protein 7B2 gene expression in man." Paquet L., Lazure C., Seidah N.G., Chretien M., Mbikay M. Biochem. Biophys. Res. Commun. 174:156-162(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), PROTEIN SEQUENCE OF 125-140 (ISOFORM 1), PROTEIN SEQUENCE OF 126-132 (ISOFORM 2). Tissue: Pituitary.
[3]	"Human neuroendocrine protein 7B2 genomic organization." Jackson R.S. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pancreas.
[5]	"Structural organization of the gene encoding the neuroendocrine chaperone 7B2." Braks J.A., Broers C.A., Danger J.M., Martens G.J.M. Eur. J. Biochem. 236:60-67(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-92.
[6]	"Isolation and NH2-terminal sequence of a highly conserved human and porcine pituitary protein belonging to a new superfamily. Immunocytochemical localization in pars distalis and pars nervosa of the pituitary and in the supraoptic nucleus of the hypothalamus." Seidah N.G., Hsi K.L., de Serres G., Rochemont J., Hamelin J., Antakly T., Cantin M., Chretien M. Arch. Biochem. Biophys. 225:525-534(1983) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-103.
[7]	"7B2 is a neuroendocrine chaperone that transiently interacts with prohormone convertase PC2 in the secretory pathway." Braks J.A., Martens G.J.M. Cell 78:263-273(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH PCSK2.
[8]	"Neuroendocrine secretory protein 7B2: structure, expression and functions." Mbikay M., Seidah N.G., Chretien M. Biochem. J. 357:329-342(2001) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00757 mRNA. Translation: CAA68726.1. AJ290438 , AJ290439, AJ290440, AJ290441, AJ290442 Genomic DNA. Translation: CAB90397.1. BC005349 mRNA. Translation: AAH05349.1.
IPI	IPI00008944. IPI00470716.
PIR	PUHU. S01008.
RefSeq	NP_001138229.1. NM_001144757.1. NP_003011.1. NM_003020.3.
UniGene	Hs.156540.
3D structure databases
ProteinModelPortal	P05408.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05408. 2 interactions.
MINT	MINT-1402757.
STRING	9606.ENSP00000300175.
Protein family/group databases
MEROPS	I21.001.
PTM databases
PhosphoSite	P05408.
Polymorphism databases
DMDM	23830842.
Proteomic databases
PaxDb	P05408.
PRIDE	P05408.
Protocols and materials databases
DNASU	6447.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000300175; ENSP00000300175; ENSG00000166922. ENST00000413748; ENSP00000388560; ENSG00000166922.
GeneID	6447.
KEGG	hsa:6447.
UCSC	uc001zha.2. human.
Organism-specific databases
CTD	6447.
GeneCards	GC15P032933.
HGNC	HGNC:10816. SCG5.
HPA	HPA013136.
MIM	173120. gene.
neXtProt	NX_P05408.
PharmGKB	PA35724.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG271457.
HOGENOM	HOG000259690.
HOVERGEN	HBG000031.
InParanoid	P05408.
OMA	GPYGNIP.
OrthoDB	EOG44MXSZ.
PhylomeDB	P05408.
Gene expression databases
ArrayExpress	P05408.
Bgee	P05408.
CleanEx	HS_SCG5.
Genevestigator	P05408.
GermOnline	ENSG00000166922. Homo sapiens.
Family and domain databases
InterPro	IPR007945. Secretogranin_V. [Graphical view]
PANTHER	PTHR12738. PTHR12738. 1 hit.
Pfam	PF05281. Secretogranin_V. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6447.
NextBio	25057.
SOURCE	Search...

Entry information

Entry name

7B2_HUMAN

Accession

Primary (citable) accession number: P05408
Secondary accession number(s): P01164, Q9BS38

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 10, 2002
Last modified:	April 3, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.