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Protein

Chloromuconate cycloisomerase

Gene

tfdDI

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(2R)-2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactori

Pathwayi: 3-chlorocatechol degradation

This protein is involved in the pathway 3-chlorocatechol degradation, which is part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the pathway 3-chlorocatechol degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei165Proton acceptorBy similarity1
Metal bindingi194ManganeseBy similarity1
Metal bindingi220Manganese1
Metal bindingi245Manganese1
Active sitei323Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14403.
UniPathwayiUPA00083.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloromuconate cycloisomerase (EC:5.5.1.7)
Alternative name(s):
Muconate cycloisomerase II
Gene namesi
Name:tfdDI
Synonyms:tfdD
Ordered Locus Names:Reut_D6465
Encoded oniPlasmid pJP46 Publications
Plasmid pReut11 Publication
OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
Proteomesi
  • UP000002697 Componenti: Plasmid pReut1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001712561 – 370Chloromuconate cycloisomeraseAdd BLAST370

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 20Combined sources16
Beta strandi25 – 38Combined sources14
Beta strandi44 – 46Combined sources3
Turni51 – 55Combined sources5
Helixi60 – 70Combined sources11
Helixi72 – 75Combined sources4
Helixi83 – 91Combined sources9
Helixi98 – 115Combined sources18
Helixi120 – 123Combined sources4
Beta strandi130 – 138Combined sources9
Helixi143 – 155Combined sources13
Beta strandi161 – 165Combined sources5
Beta strandi167 – 169Combined sources3
Helixi171 – 184Combined sources14
Turni185 – 188Combined sources4
Beta strandi189 – 194Combined sources6
Helixi202 – 212Combined sources11
Turni213 – 215Combined sources3
Beta strandi218 – 220Combined sources3
Helixi228 – 237Combined sources10
Beta strandi239 – 247Combined sources9
Helixi251 – 258Combined sources8
Turni259 – 261Combined sources3
Beta strandi264 – 266Combined sources3
Helixi270 – 273Combined sources4
Helixi276 – 289Combined sources14
Helixi302 – 312Combined sources11
Helixi326 – 329Combined sources4
Beta strandi334 – 336Combined sources3
Beta strandi343 – 347Combined sources5
Beta strandi351 – 353Combined sources3
Helixi360 – 366Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CHRX-ray3.00A1-370[»]
ProteinModelPortaliP05404.
SMRiP05404.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05404.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000185904.
KOiK01860.
OMAiYNTSTID.
OrthoDBiPOG091H04PO.

Family and domain databases

CDDicd03318. MLE. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05404-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIDAIEAVI VDVPTKRPIQ MSITTVHQQS YVIVRVYSEG LVGVGEGGSV
60 70 80 90 100
GGPVWSAECA ETIKIIVERY LAPHLLGTDA FNVSGALQTM ARAVTGNASA
110 120 130 140 150
KAAVEMALLD LKARALGVSI AELLGGPLRS AIPIAWTLAS GDTKRDLDSA
160 170 180 190 200
VEMIERRRHN RFKVKLGFRS PQDDLIHMEA LSNSLGSKAY LRVDVNQAWD
210 220 230 240 250
EQVASVYIPE LEALGVELIE QPVGRENTQA LRRLSDNNRV AIMADESLST
260 270 280 290 300
LASAFDLARD RSVDVFSLKL CNMGGVSATQ KIAAVAEASG IASYGGTMLD
310 320 330 340 350
STIGTSVALQ LYSTVPSLPF GCELIGPFVL ADTLSHEPLE IRDYELQVPT
360 370
GVGHGMTLDE DKVRQYARVS
Length:370
Mass (Da):39,722
Last modified:August 1, 1992 - v4
Checksum:iC08032835D42EBDD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti306 – 325SVALQ…GCELI → RLHSAYLRFHASVRLRTV in AAA98267 (PubMed:2583528).CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35097 Genomic DNA. Translation: AAA98263.1.
M31458 Genomic DNA. Translation: AAA98267.1.
AY365053 Genomic DNA. Translation: AAR31038.1.
CP000093 Genomic DNA. Translation: AAZ65763.1.
PIRiB35255.
RefSeqiWP_011178385.1. NC_007337.1.

Genome annotation databases

EnsemblBacteriaiAAZ65763; AAZ65763; Reut_D6465.
KEGGireu:Reut_D6465.
PATRICi20225306. VBIRalEut24049_0532.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35097 Genomic DNA. Translation: AAA98263.1.
M31458 Genomic DNA. Translation: AAA98267.1.
AY365053 Genomic DNA. Translation: AAR31038.1.
CP000093 Genomic DNA. Translation: AAZ65763.1.
PIRiB35255.
RefSeqiWP_011178385.1. NC_007337.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CHRX-ray3.00A1-370[»]
ProteinModelPortaliP05404.
SMRiP05404.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ65763; AAZ65763; Reut_D6465.
KEGGireu:Reut_D6465.
PATRICi20225306. VBIRalEut24049_0532.

Phylogenomic databases

HOGENOMiHOG000185904.
KOiK01860.
OMAiYNTSTID.
OrthoDBiPOG091H04PO.

Enzyme and pathway databases

UniPathwayiUPA00083.
BioCyciMetaCyc:MONOMER-14403.

Miscellaneous databases

EvolutionaryTraceiP05404.

Family and domain databases

CDDicd03318. MLE. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N-like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF13378. MR_MLE_C. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTFDD1_CUPPJ
AccessioniPrimary (citable) accession number: P05404
Secondary accession number(s): Q46M67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 136 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Chloromuconate cycloisomerase II is highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.