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P05404

- TFDD1_CUPPJ

UniProt

P05404 - TFDD1_CUPPJ

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Protein
Chloromuconate cycloisomerase
Gene
tfdDI, tfdD, Reut_D6465
Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactori

Manganese.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei165 – 1651Proton acceptor By similarity
Metal bindingi194 – 1941Manganese By similarity
Metal bindingi220 – 2201Manganese
Metal bindingi245 – 2451Manganese
Active sitei323 – 3231Proton donor By similarity

GO - Molecular functioni

  1. chloromuconate cycloisomerase activity Source: UniProtKB-EC
  2. manganese ion binding Source: InterPro
  3. muconate cycloisomerase activity Source: InterPro

GO - Biological processi

  1. aromatic compound catabolic process Source: UniProtKB-KW
  2. cellular amino acid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciCPIN264198:GIW3-6551-MONOMER.
MetaCyc:MONOMER-14403.
UniPathwayiUPA00083.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloromuconate cycloisomerase (EC:5.5.1.7)
Alternative name(s):
Muconate cycloisomerase II
Gene namesi
Name:tfdDI
Synonyms:tfdD
Ordered Locus Names:Reut_D6465
Encoded oniPlasmid pJP46 Publications
Plasmid pReut11 Publication
OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Taxonomic identifieri264198 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
ProteomesiUP000002697: Plasmid pReut1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Chloromuconate cycloisomerase
PRO_0000171256Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264198.Reut_D6465.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 2016
Beta strandi25 – 3814
Beta strandi44 – 463
Turni51 – 555
Helixi60 – 7011
Helixi72 – 754
Helixi83 – 919
Helixi98 – 11518
Helixi120 – 1234
Beta strandi130 – 1389
Helixi143 – 15513
Beta strandi161 – 1655
Beta strandi167 – 1693
Helixi171 – 18414
Turni185 – 1884
Beta strandi189 – 1946
Helixi202 – 21211
Turni213 – 2153
Beta strandi218 – 2203
Helixi228 – 23710
Beta strandi239 – 2479
Helixi251 – 2588
Turni259 – 2613
Beta strandi264 – 2663
Helixi270 – 2734
Helixi276 – 28914
Helixi302 – 31211
Helixi326 – 3294
Beta strandi334 – 3363
Beta strandi343 – 3475
Beta strandi351 – 3533
Helixi360 – 3667

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CHRX-ray3.00A1-370[»]
ProteinModelPortaliP05404.
SMRiP05404. Positions 1-370.

Miscellaneous databases

EvolutionaryTraceiP05404.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG4948.
HOGENOMiHOG000185904.
KOiK01860.
OMAiYPNDISA.
OrthoDBiEOG6QG8F7.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProiIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05404-1 [UniParc]FASTAAdd to Basket

« Hide

MKIDAIEAVI VDVPTKRPIQ MSITTVHQQS YVIVRVYSEG LVGVGEGGSV    50
GGPVWSAECA ETIKIIVERY LAPHLLGTDA FNVSGALQTM ARAVTGNASA 100
KAAVEMALLD LKARALGVSI AELLGGPLRS AIPIAWTLAS GDTKRDLDSA 150
VEMIERRRHN RFKVKLGFRS PQDDLIHMEA LSNSLGSKAY LRVDVNQAWD 200
EQVASVYIPE LEALGVELIE QPVGRENTQA LRRLSDNNRV AIMADESLST 250
LASAFDLARD RSVDVFSLKL CNMGGVSATQ KIAAVAEASG IASYGGTMLD 300
STIGTSVALQ LYSTVPSLPF GCELIGPFVL ADTLSHEPLE IRDYELQVPT 350
GVGHGMTLDE DKVRQYARVS 370
Length:370
Mass (Da):39,722
Last modified:August 1, 1992 - v4
Checksum:iC08032835D42EBDD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti306 – 32520SVALQ…GCELI → RLHSAYLRFHASVRLRTV in AAA98267. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35097 Genomic DNA. Translation: AAA98263.1.
M31458 Genomic DNA. Translation: AAA98267.1.
AY365053 Genomic DNA. Translation: AAR31038.1.
CP000093 Genomic DNA. Translation: AAZ65763.1.
PIRiB35255.
RefSeqiWP_011178385.1. NC_007337.1.
YP_293620.1. NC_007337.1.

Genome annotation databases

EnsemblBacteriaiAAZ65763; AAZ65763; Reut_D6465.
GeneIDi3607311.
KEGGireu:Reut_D6465.
PATRICi20225306. VBIRalEut24049_0532.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35097 Genomic DNA. Translation: AAA98263.1 .
M31458 Genomic DNA. Translation: AAA98267.1 .
AY365053 Genomic DNA. Translation: AAR31038.1 .
CP000093 Genomic DNA. Translation: AAZ65763.1 .
PIRi B35255.
RefSeqi WP_011178385.1. NC_007337.1.
YP_293620.1. NC_007337.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2CHR X-ray 3.00 A 1-370 [» ]
ProteinModelPortali P05404.
SMRi P05404. Positions 1-370.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 264198.Reut_D6465.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ65763 ; AAZ65763 ; Reut_D6465 .
GeneIDi 3607311.
KEGGi reu:Reut_D6465.
PATRICi 20225306. VBIRalEut24049_0532.

Phylogenomic databases

eggNOGi COG4948.
HOGENOMi HOG000185904.
KOi K01860.
OMAi YPNDISA.
OrthoDBi EOG6QG8F7.

Enzyme and pathway databases

UniPathwayi UPA00083 .
BioCyci CPIN264198:GIW3-6551-MONOMER.
MetaCyc:MONOMER-14403.

Miscellaneous databases

EvolutionaryTracei P05404.

Family and domain databases

Gene3Di 3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProi IPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view ]
PANTHERi PTHR13794. PTHR13794. 1 hit.
Pfami PF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view ]
SMARTi SM00922. MR_MLE. 1 hit.
[Graphical view ]
SUPFAMi SSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsi TIGR02534. mucon_cyclo. 1 hit.
PROSITEi PS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4."
    Perkins E.J., Gordon M.P., Caceres O., Lurquin P.F.
    J. Bacteriol. 172:2351-2359(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pJP4
  2. "Operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pJP4 and pAC27."
    Ghosal D., You I.-S.
    Gene 83:225-232(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pJP4
  3. "Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways."
    Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B.
    Environ. Microbiol. 6:655-668(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pJP4
  4. "Complete sequence of plasmid 1 of Ralstonia eutropha JMP134."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JMP134 / LMG 1197.
    Plasmid: pReut1
  5. "Partial nucleotide sequence of the chlorocatechol degradative operon tfdCDEF of pJP4 and similarity to promoters of the chlorinated aromatic degradative operons tfdA and clcABD."
    Perkins E.J., Bolton G., Gordon M.P., Lurquin P.F.
    Nucleic Acids Res. 16:7200-7200(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-166.
    Plasmid: pJP4
  6. "Crystal structure of chloromuconate cycloisomerase from Alcaligenes eutrophus JMP134 (pJP4) at 3-A resolution."
    Hoier H., Schloemann M., Hammer A., Glusker J.P., Carrell H.L., Goldman A., Stezowski J.J., Heinemann U.
    Acta Crystallogr. D 50:75-84(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH MANGANESE IONS.
    Plasmid: pJP4
  7. "A re-evaluation of the crystal structure of chloromuconate cycloisomerase."
    Kleywegt G.J., Jones T.A.
    Acta Crystallogr. D 52:858-863(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
    Plasmid: pJP4

Entry informationi

Entry nameiTFDD1_CUPPJ
AccessioniPrimary (citable) accession number: P05404
Secondary accession number(s): Q46M67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: August 1, 1992
Last modified: September 3, 2014
This is version 120 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Chloromuconate cycloisomerase II is highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

Keywords - Technical termi

3D-structure, Complete proteome, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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