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P05404 (TFDD1_CUPPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloromuconate cycloisomerase

EC=5.5.1.7
Alternative name(s):
Muconate cycloisomerase II
Gene names
Name:tfdDI
Synonyms:tfdD
Ordered Locus Names:Reut_D6465
Encoded onPlasmid pJP4 Ref.1 Ref.2 Ref.3 Ref.5 Ref.6 Ref.7
Plasmid pReut1 Ref.4
OrganismCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134)) [Complete proteome] [HAMAP]
Taxonomic identifier264198 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

Cofactor

Manganese.

Pathway

Aromatic compound metabolism; 3-chlorocatechol degradation.

Miscellaneous

Chloromuconate cycloisomerase II is highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

Sequence similarities

Belongs to the mandelate racemase/muconate lactonizing enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Chloromuconate cycloisomerase
PRO_0000171256

Sites

Active site1651Proton acceptor By similarity
Active site3231Proton donor By similarity
Metal binding1941Manganese By similarity
Metal binding2201Manganese
Metal binding2451Manganese

Experimental info

Sequence conflict306 – 32520SVALQ…GCELI → RLHSAYLRFHASVRLRTV in AAA98267. Ref.2

Secondary structure

............................................................. 370
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P05404 [UniParc].

Last modified August 1, 1992. Version 4.
Checksum: C08032835D42EBDD

FASTA37039,722
        10         20         30         40         50         60 
MKIDAIEAVI VDVPTKRPIQ MSITTVHQQS YVIVRVYSEG LVGVGEGGSV GGPVWSAECA 

        70         80         90        100        110        120 
ETIKIIVERY LAPHLLGTDA FNVSGALQTM ARAVTGNASA KAAVEMALLD LKARALGVSI 

       130        140        150        160        170        180 
AELLGGPLRS AIPIAWTLAS GDTKRDLDSA VEMIERRRHN RFKVKLGFRS PQDDLIHMEA 

       190        200        210        220        230        240 
LSNSLGSKAY LRVDVNQAWD EQVASVYIPE LEALGVELIE QPVGRENTQA LRRLSDNNRV 

       250        260        270        280        290        300 
AIMADESLST LASAFDLARD RSVDVFSLKL CNMGGVSATQ KIAAVAEASG IASYGGTMLD 

       310        320        330        340        350        360 
STIGTSVALQ LYSTVPSLPF GCELIGPFVL ADTLSHEPLE IRDYELQVPT GVGHGMTLDE 

       370 
DKVRQYARVS 

« Hide

References

« Hide 'large scale' references
[1]"Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4."
Perkins E.J., Gordon M.P., Caceres O., Lurquin P.F.
J. Bacteriol. 172:2351-2359(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pJP4 and pAC27."
Ghosal D., You I.-S.
Gene 83:225-232(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways."
Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B.
Environ. Microbiol. 6:655-668(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete sequence of plasmid 1 of Ralstonia eutropha JMP134."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JMP134 / LMG 1197.
[5]"Partial nucleotide sequence of the chlorocatechol degradative operon tfdCDEF of pJP4 and similarity to promoters of the chlorinated aromatic degradative operons tfdA and clcABD."
Perkins E.J., Bolton G., Gordon M.P., Lurquin P.F.
Nucleic Acids Res. 16:7200-7200(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-166.
[6]"Crystal structure of chloromuconate cycloisomerase from Alcaligenes eutrophus JMP134 (pJP4) at 3-A resolution."
Hoier H., Schloemann M., Hammer A., Glusker J.P., Carrell H.L., Goldman A., Stezowski J.J., Heinemann U.
Acta Crystallogr. D 50:75-84(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH MANGANESE IONS.
[7]"A re-evaluation of the crystal structure of chloromuconate cycloisomerase."
Kleywegt G.J., Jones T.A.
Acta Crystallogr. D 52:858-863(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35097 Genomic DNA. Translation: AAA98263.1.
M31458 Genomic DNA. Translation: AAA98267.1.
AY365053 Genomic DNA. Translation: AAR31038.1.
CP000093 Genomic DNA. Translation: AAZ65763.1.
PIRB35255.
RefSeqYP_293620.1. NC_007337.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CHRX-ray3.00A1-370[»]
ProteinModelPortalP05404.
SMRP05404. Positions 1-370.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING264198.Reut_D6465.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ65763; AAZ65763; Reut_D6465.
GeneID3607311.
KEGGreu:Reut_D6465.
PATRIC20225306. VBIRalEut24049_0532.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4948.
HOGENOMHOG000185904.
KOK01860.
OMAYPNDISA.
OrthoDBEOG6QG8F7.

Enzyme and pathway databases

BioCycCPIN264198:GIW3-6551-MONOMER.
MetaCyc:MONOMER-14403.
UniPathwayUPA00083.

Family and domain databases

Gene3D3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
InterProIPR013370. Chloromuconate_cycloisomerase.
IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N.
IPR001354. MR_MLE.
[Graphical view]
PANTHERPTHR13794. PTHR13794. 1 hit.
PfamPF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsTIGR02534. mucon_cyclo. 1 hit.
PROSITEPS00908. MR_MLE_1. 1 hit.
PS00909. MR_MLE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP05404.

Entry information

Entry nameTFDD1_CUPPJ
AccessionPrimary (citable) accession number: P05404
Secondary accession number(s): Q46M67
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: August 1, 1992
Last modified: June 11, 2014
This is version 119 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways