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P05404

- TFDD1_CUPPJ

UniProt

P05404 - TFDD1_CUPPJ

Protein

Chloromuconate cycloisomerase

Gene

tfdDI

Organism
Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 4 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate.

    Cofactori

    Manganese.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei165 – 1651Proton acceptorBy similarity
    Metal bindingi194 – 1941ManganeseBy similarity
    Metal bindingi220 – 2201Manganese
    Metal bindingi245 – 2451Manganese
    Active sitei323 – 3231Proton donorBy similarity

    GO - Molecular functioni

    1. chloromuconate cycloisomerase activity Source: UniProtKB-EC
    2. manganese ion binding Source: InterPro
    3. muconate cycloisomerase activity Source: InterPro

    GO - Biological processi

    1. aromatic compound catabolic process Source: UniProtKB-KW
    2. cellular amino acid catabolic process Source: InterPro

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciCPIN264198:GIW3-6551-MONOMER.
    MetaCyc:MONOMER-14403.
    UniPathwayiUPA00083.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chloromuconate cycloisomerase (EC:5.5.1.7)
    Alternative name(s):
    Muconate cycloisomerase II
    Gene namesi
    Name:tfdDI
    Synonyms:tfdD
    Ordered Locus Names:Reut_D6465
    Encoded oniPlasmid pJP46 Publications
    Plasmid pReut11 Publication
    OrganismiCupriavidus pinatubonensis (strain JMP 134 / LMG 1197) (Ralstonia eutropha (strain JMP 134))
    Taxonomic identifieri264198 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000002697: Plasmid pReut1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Chloromuconate cycloisomerasePRO_0000171256Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi264198.Reut_D6465.

    Structurei

    Secondary structure

    1
    370
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 2016
    Beta strandi25 – 3814
    Beta strandi44 – 463
    Turni51 – 555
    Helixi60 – 7011
    Helixi72 – 754
    Helixi83 – 919
    Helixi98 – 11518
    Helixi120 – 1234
    Beta strandi130 – 1389
    Helixi143 – 15513
    Beta strandi161 – 1655
    Beta strandi167 – 1693
    Helixi171 – 18414
    Turni185 – 1884
    Beta strandi189 – 1946
    Helixi202 – 21211
    Turni213 – 2153
    Beta strandi218 – 2203
    Helixi228 – 23710
    Beta strandi239 – 2479
    Helixi251 – 2588
    Turni259 – 2613
    Beta strandi264 – 2663
    Helixi270 – 2734
    Helixi276 – 28914
    Helixi302 – 31211
    Helixi326 – 3294
    Beta strandi334 – 3363
    Beta strandi343 – 3475
    Beta strandi351 – 3533
    Helixi360 – 3667

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CHRX-ray3.00A1-370[»]
    ProteinModelPortaliP05404.
    SMRiP05404. Positions 1-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05404.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG4948.
    HOGENOMiHOG000185904.
    KOiK01860.
    OMAiYPNDISA.
    OrthoDBiEOG6QG8F7.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProiIPR013370. Chloromuconate_cycloisomerase.
    IPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR02534. mucon_cyclo. 1 hit.
    PROSITEiPS00908. MR_MLE_1. 1 hit.
    PS00909. MR_MLE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05404-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIDAIEAVI VDVPTKRPIQ MSITTVHQQS YVIVRVYSEG LVGVGEGGSV    50
    GGPVWSAECA ETIKIIVERY LAPHLLGTDA FNVSGALQTM ARAVTGNASA 100
    KAAVEMALLD LKARALGVSI AELLGGPLRS AIPIAWTLAS GDTKRDLDSA 150
    VEMIERRRHN RFKVKLGFRS PQDDLIHMEA LSNSLGSKAY LRVDVNQAWD 200
    EQVASVYIPE LEALGVELIE QPVGRENTQA LRRLSDNNRV AIMADESLST 250
    LASAFDLARD RSVDVFSLKL CNMGGVSATQ KIAAVAEASG IASYGGTMLD 300
    STIGTSVALQ LYSTVPSLPF GCELIGPFVL ADTLSHEPLE IRDYELQVPT 350
    GVGHGMTLDE DKVRQYARVS 370
    Length:370
    Mass (Da):39,722
    Last modified:August 1, 1992 - v4
    Checksum:iC08032835D42EBDD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti306 – 32520SVALQ…GCELI → RLHSAYLRFHASVRLRTV in AAA98267. (PubMed:2583528)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35097 Genomic DNA. Translation: AAA98263.1.
    M31458 Genomic DNA. Translation: AAA98267.1.
    AY365053 Genomic DNA. Translation: AAR31038.1.
    CP000093 Genomic DNA. Translation: AAZ65763.1.
    PIRiB35255.
    RefSeqiWP_011178385.1. NC_007337.1.
    YP_293620.1. NC_007337.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ65763; AAZ65763; Reut_D6465.
    GeneIDi3607311.
    KEGGireu:Reut_D6465.
    PATRICi20225306. VBIRalEut24049_0532.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35097 Genomic DNA. Translation: AAA98263.1 .
    M31458 Genomic DNA. Translation: AAA98267.1 .
    AY365053 Genomic DNA. Translation: AAR31038.1 .
    CP000093 Genomic DNA. Translation: AAZ65763.1 .
    PIRi B35255.
    RefSeqi WP_011178385.1. NC_007337.1.
    YP_293620.1. NC_007337.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CHR X-ray 3.00 A 1-370 [» ]
    ProteinModelPortali P05404.
    SMRi P05404. Positions 1-370.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 264198.Reut_D6465.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ65763 ; AAZ65763 ; Reut_D6465 .
    GeneIDi 3607311.
    KEGGi reu:Reut_D6465.
    PATRICi 20225306. VBIRalEut24049_0532.

    Phylogenomic databases

    eggNOGi COG4948.
    HOGENOMi HOG000185904.
    KOi K01860.
    OMAi YPNDISA.
    OrthoDBi EOG6QG8F7.

    Enzyme and pathway databases

    UniPathwayi UPA00083 .
    BioCyci CPIN264198:GIW3-6551-MONOMER.
    MetaCyc:MONOMER-14403.

    Miscellaneous databases

    EvolutionaryTracei P05404.

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    InterProi IPR013370. Chloromuconate_cycloisomerase.
    IPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    [Graphical view ]
    PANTHERi PTHR13794. PTHR13794. 1 hit.
    Pfami PF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view ]
    SMARTi SM00922. MR_MLE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsi TIGR02534. mucon_cyclo. 1 hit.
    PROSITEi PS00908. MR_MLE_1. 1 hit.
    PS00909. MR_MLE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4."
      Perkins E.J., Gordon M.P., Caceres O., Lurquin P.F.
      J. Bacteriol. 172:2351-2359(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: pJP4
    2. "Operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pJP4 and pAC27."
      Ghosal D., You I.-S.
      Gene 83:225-232(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: pJP4
    3. "Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways."
      Trefault N., De la Iglesia R., Molina A.M., Manzano M., Ledger T., Perez-Pantoja D., Sanchez M.A., Stuardo M., Gonzalez B.
      Environ. Microbiol. 6:655-668(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: pJP4
    4. "Complete sequence of plasmid 1 of Ralstonia eutropha JMP134."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Goltsman E., Martinez M., Schmutz J., Larimer F., Land M., Lykidis A., Richardson P.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JMP134 / LMG 1197.
      Plasmid: pReut1
    5. "Partial nucleotide sequence of the chlorocatechol degradative operon tfdCDEF of pJP4 and similarity to promoters of the chlorinated aromatic degradative operons tfdA and clcABD."
      Perkins E.J., Bolton G., Gordon M.P., Lurquin P.F.
      Nucleic Acids Res. 16:7200-7200(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-166.
      Plasmid: pJP4
    6. "Crystal structure of chloromuconate cycloisomerase from Alcaligenes eutrophus JMP134 (pJP4) at 3-A resolution."
      Hoier H., Schloemann M., Hammer A., Glusker J.P., Carrell H.L., Goldman A., Stezowski J.J., Heinemann U.
      Acta Crystallogr. D 50:75-84(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) IN COMPLEX WITH MANGANESE IONS.
      Plasmid: pJP4
    7. "A re-evaluation of the crystal structure of chloromuconate cycloisomerase."
      Kleywegt G.J., Jones T.A.
      Acta Crystallogr. D 52:858-863(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
      Plasmid: pJP4

    Entry informationi

    Entry nameiTFDD1_CUPPJ
    AccessioniPrimary (citable) accession number: P05404
    Secondary accession number(s): Q46M67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 121 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Chloromuconate cycloisomerase II is highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates.

    Keywords - Technical termi

    3D-structure, Complete proteome, Plasmid

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3