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Protein

Enzymatic polyprotein

Gene

ORF V

Organism
Carnation etched ring virus (CERV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Encodes for at least two polypeptides: protease (PR) and reverse transcriptase (RT). The protease processes the polyprotein in cis. Reverse transcriptase is multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated, and reverse-transcribed inside the nucleocapsid. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA) (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei341

GO - Molecular functioni

Keywordsi

Molecular functionAspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Protein family/group databases

MEROPSiA03.001

Names & Taxonomyi

Protein namesi
Recommended name:
Enzymatic polyprotein
Including the following 3 domains:
Aspartic protease (EC:3.4.23.-)
Endonuclease
Reverse transcriptase (EC:2.7.7.49)
Gene namesi
ORF Names:ORF V
OrganismiCarnation etched ring virus (CERV)
Taxonomic identifieri10640 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesCaulimoviridaeCaulimovirus
Virus hostiDianthus caryophyllus (Carnation) (Clove pink) [TaxID: 3570]
Proteomesi
  • UP000008446 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002220551 – 659Enzymatic polyproteinAdd BLAST659

Proteomic databases

PRIDEiP05400

Structurei

3D structure databases

ProteinModelPortaliP05400
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini252 – 436Reverse transcriptasePROSITE-ProRule annotationAdd BLAST185

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 180ProteaseBy similarityAdd BLAST180

Domaini

The polymerase/reverse transcriptase (RT) and ribonuclease H (RH) domains are structured in five subdomains: finger, palm, thumb, connection and RNase H. Within the palm subdomain, the 'primer grip' region is thought to be involved in the positioning of the primer terminus for accommodating the incoming nucleotide. The RH domain stabilizes the association of RT with primer-template (By similarity).By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di2.40.70.10, 1 hit
InterProiView protein in InterPro
IPR000588 Pept_A3A
IPR021109 Peptidase_aspartic_dom_sf
IPR000477 RT_dom
PfamiView protein in Pfam
PF02160 Peptidase_A3, 1 hit
PF00078 RVT_1, 1 hit
PRINTSiPR00731 CAULIMOPTASE
PROSITEiView protein in PROSITE
PS50878 RT_POL, 1 hit

Sequencei

Sequence statusi: Complete.

P05400-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRNRTNPN SIYVKGILKF PGYQTNLDLH CYVDTGSSLC MASKYVIPEE
60 70 80 90 100
YWQTAEKPLN IKIANGKIIQ LTKVCSKLPI RLGGERFLIP TLFQQESGID
110 120 130 140 150
LLLGNNFCQL YSPFIQYTDR IYFHLNKQSV IIGKITKAYQ YGVKGFLESM
160 170 180 190 200
KKKSKVNRPE PINITSNQHL FLEEGGNHVD EMLYEIQISK FSAIEEMLER
210 220 230 240 250
VSSENPIDPE KSKQWMTATI ELIDPKTVVK VKPMSYSPSD REEFDRQIKE
260 270 280 290 300
LLELKVIKPS KSTHMSPAFL VENEAERRRG KKRMVVNYKA MNKATKGDAH
310 320 330 340 350
NLPNKDELLT LVRGKKIYSS FDCKSGLWQV LLDKESQLLT AFTCPQGHYQ
360 370 380 390 400
WNVVPFGLKQ APSIFPKTYA NSHSNQYSKY CCVYVDDILV FSNTGRKEHY
410 420 430 440 450
IHVLNILRRC EKLGIILSKK KAQLFKEKIN FLGLEIDQGT HCPQNHILEH
460 470 480 490 500
IHKFPDRIED KKQLQRFLGI LTYASDYIPK LASIRKPLQS KLKEDSTWTW
510 520 530 540 550
NDTDSQYMAK IKKNLKSFPK LYHPEPNDKL VIETDASEEF WGGILKAIHN
560 570 580 590 600
SHEYICRYAS GSFKAAERNY HSNEKELLAV IRVIKKFSIY LTPSRFLIRT
610 620 630 640 650
DNKNFTHFVN INLKGDRKQG RLVRWQMWLS QYDFDVEHIA GTKNVFADFL

QENTLTNYV
Length:659
Mass (Da):76,517
Last modified:November 1, 1988 - v1
Checksum:i25CC30475E38B3C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04658 Genomic DNA Translation: CAA28360.1
PIRiS00854
RefSeqiNP_612577.1, NC_003498.1

Genome annotation databases

GeneIDi935429
KEGGivg:935429

Similar proteinsi

Entry informationi

Entry nameiPOL_CERV
AccessioniPrimary (citable) accession number: P05400
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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