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P05400 (POL_CERV) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Enzymatic polyprotein

Including the following 3 domains:

  1. Aspartic protease
    EC=3.4.23.-
  2. Endonuclease
  3. Reverse transcriptase
    EC=2.7.7.49
Gene names
ORF Names:ORF V
OrganismCarnation etched ring virus (CERV) [Complete proteome]
Taxonomic identifier10640 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesCaulimoviridaeCaulimovirus
Virus hostDianthus caryophyllus (Carnation) (Clove pink) [TaxID: 3570]

Protein attributes

Sequence length659 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Encodes for at least two polypeptides: protease (PR) and reverse transcriptase (RT). The protease processes the polyprotein in cis. Reverse transcriptase is multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated, and reverse-transcribed inside the nucleocapsid. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA) By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Domain

The polymerase/reverse transcriptase (RT) and ribonuclease H (RH) domains are structured in five subdomains: finger, palm, thumb, connection and RNase H. Within the palm subdomain, the 'primer grip' region is thought to be involved in the positioning of the primer terminus for accommodating the incoming nucleotide. The RH domain stabilizes the association of RT with primer-template By similarity.

Sequence similarities

Belongs to the caulimoviridae enzymatic polyprotein family.

Contains 1 peptidase A3A domain.

Contains 1 reverse transcriptase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 659659Enzymatic polyprotein
PRO_0000222055

Regions

Domain252 – 436185Reverse transcriptase
Region1 – 180180Protease By similarity

Sites

Active site341

Sequences

Sequence LengthMass (Da)Tools
P05400 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 25CC30475E38B3C6

FASTA65976,517
        10         20         30         40         50         60 
MSLRNRTNPN SIYVKGILKF PGYQTNLDLH CYVDTGSSLC MASKYVIPEE YWQTAEKPLN 

        70         80         90        100        110        120 
IKIANGKIIQ LTKVCSKLPI RLGGERFLIP TLFQQESGID LLLGNNFCQL YSPFIQYTDR 

       130        140        150        160        170        180 
IYFHLNKQSV IIGKITKAYQ YGVKGFLESM KKKSKVNRPE PINITSNQHL FLEEGGNHVD 

       190        200        210        220        230        240 
EMLYEIQISK FSAIEEMLER VSSENPIDPE KSKQWMTATI ELIDPKTVVK VKPMSYSPSD 

       250        260        270        280        290        300 
REEFDRQIKE LLELKVIKPS KSTHMSPAFL VENEAERRRG KKRMVVNYKA MNKATKGDAH 

       310        320        330        340        350        360 
NLPNKDELLT LVRGKKIYSS FDCKSGLWQV LLDKESQLLT AFTCPQGHYQ WNVVPFGLKQ 

       370        380        390        400        410        420 
APSIFPKTYA NSHSNQYSKY CCVYVDDILV FSNTGRKEHY IHVLNILRRC EKLGIILSKK 

       430        440        450        460        470        480 
KAQLFKEKIN FLGLEIDQGT HCPQNHILEH IHKFPDRIED KKQLQRFLGI LTYASDYIPK 

       490        500        510        520        530        540 
LASIRKPLQS KLKEDSTWTW NDTDSQYMAK IKKNLKSFPK LYHPEPNDKL VIETDASEEF 

       550        560        570        580        590        600 
WGGILKAIHN SHEYICRYAS GSFKAAERNY HSNEKELLAV IRVIKKFSIY LTPSRFLIRT 

       610        620        630        640        650 
DNKNFTHFVN INLKGDRKQG RLVRWQMWLS QYDFDVEHIA GTKNVFADFL QENTLTNYV 

« Hide

References

[1]"The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses."
Hull R., Sadler J., Longstaff M.
EMBO J. 5:3083-3090(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04658 Genomic DNA. Translation: CAA28360.1.
PIRS00854.
RefSeqNP_612577.1. NC_003498.1.

3D structure databases

ProteinModelPortalP05400.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSA03.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID935429.

Family and domain databases

InterProIPR000588. Pept_A3A.
IPR000477. RT_dom.
[Graphical view]
PfamPF02160. Peptidase_A3. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
PRINTSPR00731. CAULIMOPTASE.
PROSITEPS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePOL_CERV
AccessionPrimary (citable) accession number: P05400
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries