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Protein

Enzymatic polyprotein

Gene

ORF V

Organism
Carnation etched ring virus (CERV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Encodes for at least two polypeptides: protease (PR) and reverse transcriptase (RT). The protease processes the polyprotein in cis. Reverse transcriptase is multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated, and reverse-transcribed inside the nucleocapsid. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA) (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. endonuclease activity Source: UniProtKB-KW
  3. RNA-directed DNA polymerase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Protein family/group databases

MEROPSiA03.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Enzymatic polyprotein
Including the following 3 domains:
Aspartic protease (EC:3.4.23.-)
Endonuclease
Reverse transcriptase (EC:2.7.7.49)
Gene namesi
ORF Names:ORF V
OrganismiCarnation etched ring virus (CERV)
Taxonomic identifieri10640 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesCaulimoviridaeCaulimovirus
Virus hostiDianthus caryophyllus (Carnation) (Clove pink) [TaxID: 3570]
ProteomesiUP000008446: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 659659Enzymatic polyproteinPRO_0000222055Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP05400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini252 – 436185Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 180180ProteaseBy similarityAdd
BLAST

Domaini

The polymerase/reverse transcriptase (RT) and ribonuclease H (RH) domains are structured in five subdomains: finger, palm, thumb, connection and RNase H. Within the palm subdomain, the 'primer grip' region is thought to be involved in the positioning of the primer terminus for accommodating the incoming nucleotide. The RH domain stabilizes the association of RT with primer-template (By similarity).By similarity

Sequence similaritiesi

Contains 1 peptidase A3A domain.Curated
Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR000588. Pept_A3A.
IPR000477. RT_dom.
[Graphical view]
PfamiPF02160. Peptidase_A3. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
PRINTSiPR00731. CAULIMOPTASE.
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05400-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLRNRTNPN SIYVKGILKF PGYQTNLDLH CYVDTGSSLC MASKYVIPEE
60 70 80 90 100
YWQTAEKPLN IKIANGKIIQ LTKVCSKLPI RLGGERFLIP TLFQQESGID
110 120 130 140 150
LLLGNNFCQL YSPFIQYTDR IYFHLNKQSV IIGKITKAYQ YGVKGFLESM
160 170 180 190 200
KKKSKVNRPE PINITSNQHL FLEEGGNHVD EMLYEIQISK FSAIEEMLER
210 220 230 240 250
VSSENPIDPE KSKQWMTATI ELIDPKTVVK VKPMSYSPSD REEFDRQIKE
260 270 280 290 300
LLELKVIKPS KSTHMSPAFL VENEAERRRG KKRMVVNYKA MNKATKGDAH
310 320 330 340 350
NLPNKDELLT LVRGKKIYSS FDCKSGLWQV LLDKESQLLT AFTCPQGHYQ
360 370 380 390 400
WNVVPFGLKQ APSIFPKTYA NSHSNQYSKY CCVYVDDILV FSNTGRKEHY
410 420 430 440 450
IHVLNILRRC EKLGIILSKK KAQLFKEKIN FLGLEIDQGT HCPQNHILEH
460 470 480 490 500
IHKFPDRIED KKQLQRFLGI LTYASDYIPK LASIRKPLQS KLKEDSTWTW
510 520 530 540 550
NDTDSQYMAK IKKNLKSFPK LYHPEPNDKL VIETDASEEF WGGILKAIHN
560 570 580 590 600
SHEYICRYAS GSFKAAERNY HSNEKELLAV IRVIKKFSIY LTPSRFLIRT
610 620 630 640 650
DNKNFTHFVN INLKGDRKQG RLVRWQMWLS QYDFDVEHIA GTKNVFADFL

QENTLTNYV
Length:659
Mass (Da):76,517
Last modified:November 1, 1988 - v1
Checksum:i25CC30475E38B3C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04658 Genomic DNA. Translation: CAA28360.1.
PIRiS00854.
RefSeqiNP_612577.1. NC_003498.1.

Genome annotation databases

GeneIDi935429.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04658 Genomic DNA. Translation: CAA28360.1.
PIRiS00854.
RefSeqiNP_612577.1. NC_003498.1.

3D structure databases

ProteinModelPortaliP05400.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiA03.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi935429.

Family and domain databases

InterProiIPR000588. Pept_A3A.
IPR000477. RT_dom.
[Graphical view]
PfamiPF02160. Peptidase_A3. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
PRINTSiPR00731. CAULIMOPTASE.
PROSITEiPS50878. RT_POL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The sequence of carnation etched ring virus DNA: comparison with cauliflower mosaic virus and retroviruses."
    Hull R., Sadler J., Longstaff M.
    EMBO J. 5:3083-3090(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPOL_CERV
AccessioniPrimary (citable) accession number: P05400
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.