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Protein

Dihydropteroate synthase

Gene

sulA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.By similarity

Miscellaneous

The sequence shown here is that of sul-s (wild-type). The protein of the spontaneous mutation to sulfonamide resistance (sul-d) has an insert of 2 AA.

Catalytic activityi

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Dihydropteroate synthase (sulA)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17MagnesiumBy similarity1
Binding sitei576-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei916-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei1106-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei2016-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei2376-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processFolate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

SABIO-RKP05382
UniPathwayiUPA00077; UER00156

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase (EC:2.5.1.15)
Short name:
DHPS
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene namesi
Name:sulA
Ordered Locus Names:SP_0289
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001682301 – 314Dihydropteroate synthaseAdd BLAST314

Proteomic databases

PRIDEiP05382

Interactioni

Subunit structurei

Homodimer or homotrimer.

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiP05382, 3 interactors

Structurei

3D structure databases

ProteinModelPortaliP05382
SMRiP05382
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 294Pterin-bindingPROSITE-ProRule annotationAdd BLAST285

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni282 – 2846-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the DHPS family.Curated

Phylogenomic databases

eggNOGiENOG4105EEI Bacteria
COG0294 LUCA
HOGENOMiHOG000217509
KOiK00796
OMAiWAVRVHD

Family and domain databases

CDDicd00739 DHPS, 1 hit
Gene3Di3.20.20.20, 1 hit
InterProiView protein in InterPro
IPR006390 DHP_synth
IPR011005 Dihydropteroate_synth-like
IPR000489 Pterin-binding_dom
PfamiView protein in Pfam
PF00809 Pterin_bind, 1 hit
SUPFAMiSSF51717 SSF51717, 1 hit
TIGRFAMsiTIGR01496 DHPS, 1 hit
PROSITEiView protein in PROSITE
PS00792 DHPS_1, 1 hit
PS00793 DHPS_2, 1 hit
PS50972 PTERIN_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P05382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKANHAKT VICGIINVTP DSFSDGGQFF ALEQALQQAR KLIAEGASML
60 70 80 90 100
DIGGESTRPG SSYVEIEEEI QRVVPVIKAI RKESDVLISI DTWKSQVAEA
110 120 130 140 150
ALAAGADLVN DITGLMGDEK MAYVVAEARA KVVIMFNPVM ARPQHPSSLI
160 170 180 190 200
FPHFGFGQTF TEKELADFET LPIEDLMVAF FERALARAAE AGIAPENILL
210 220 230 240 250
DPGIGFGLTK KENLLLLRDL DKLHQKGYPI FLGVSRKRFV INILEENGFE
260 270 280 290 300
VNPETELGFR NRDTASAHVT SIAARQGVEV VRVHDVASHR MAVEIASAIR
310
LADEAENLDL KQYK
Length:314
Mass (Da):34,403
Last modified:September 26, 2001 - v2
Checksum:iF2F20D9A782083FB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122 – 123AY → PH in AAB63944 (PubMed:3114239).Curated2
Sequence conflicti131K → Q in AAB63944 (PubMed:3114239).Curated1
Sequence conflicti159T → A in AAB63944 (PubMed:3114239).Curated1
Sequence conflicti163K → E in AAB63944 (PubMed:3114239).Curated1
Sequence conflicti175D → E in AAB63944 (PubMed:3114239).Curated1
Sequence conflicti178V → E in AAB63944 (PubMed:3114239).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti67E → EIE in mutant SUL-D. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U16156 Genomic DNA Translation: AAB63944.1
AE005672 Genomic DNA Translation: AAK74467.1
PIRiA43661
B95034
B97905

Genome annotation databases

EnsemblBacteriaiAAK74467; AAK74467; SP_0289
KEGGispn:SP_0289

Similar proteinsi

Entry informationi

Entry nameiDHPS_STRPN
AccessioniPrimary (citable) accession number: P05382
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: September 26, 2001
Last modified: May 23, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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