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P05382

- DHPS_STRPN

UniProt

P05382 - DHPS_STRPN

Protein

Dihydropteroate synthase

Gene

sulA

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.

    Catalytic activityi

    (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

    Cofactori

    Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi17 – 171MagnesiumBy similarity
    Binding sitei25 – 251SubstrateBy similarity
    Binding sitei91 – 911SubstrateBy similarity
    Binding sitei110 – 1101SubstrateBy similarity
    Binding sitei201 – 2011SubstrateBy similarity
    Binding sitei237 – 2371SubstrateBy similarity
    Binding sitei282 – 2821SubstrateBy similarity
    Binding sitei284 – 2841SubstrateBy similarity

    GO - Molecular functioni

    1. dihydropteroate synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. folic acid biosynthetic process Source: UniProtKB-KW
    2. response to antibiotic Source: UniProtKB-KW
    3. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Antibiotic resistance, Folate biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciSPNE170187:GHGN-295-MONOMER.
    SABIO-RKP05382.
    UniPathwayiUPA00077; UER00156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydropteroate synthase (EC:2.5.1.15)
    Short name:
    DHPS
    Alternative name(s):
    Dihydropteroate pyrophosphorylase
    Gene namesi
    Name:sulA
    Ordered Locus Names:SP_0289
    OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
    Taxonomic identifieri170187 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000585: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314Dihydropteroate synthasePRO_0000168230Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer or homotrimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    groSQ97NV32EBI-2206997,EBI-2206949
    malRP0A4T12EBI-2206997,EBI-2207435
    SP_1069Q97QX54EBI-2206997,EBI-6472250

    Protein-protein interaction databases

    IntActiP05382. 3 interactions.
    STRINGi170187.SP_0289.

    Structurei

    3D structure databases

    ProteinModelPortaliP05382.
    SMRiP05382. Positions 7-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 294285Pterin-bindingPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni57 – 582Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DHPS family.Curated
    Contains 1 pterin-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0294.
    HOGENOMiHOG000217509.
    KOiK00796.
    OMAiDMFYVAA.
    OrthoDBiEOG67T5P5.

    Family and domain databases

    Gene3Di3.20.20.20. 1 hit.
    InterProiIPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view]
    PfamiPF00809. Pterin_bind. 1 hit.
    [Graphical view]
    SUPFAMiSSF51717. SSF51717. 1 hit.
    TIGRFAMsiTIGR01496. DHPS. 1 hit.
    PROSITEiPS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05382-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKANHAKT VICGIINVTP DSFSDGGQFF ALEQALQQAR KLIAEGASML    50
    DIGGESTRPG SSYVEIEEEI QRVVPVIKAI RKESDVLISI DTWKSQVAEA 100
    ALAAGADLVN DITGLMGDEK MAYVVAEARA KVVIMFNPVM ARPQHPSSLI 150
    FPHFGFGQTF TEKELADFET LPIEDLMVAF FERALARAAE AGIAPENILL 200
    DPGIGFGLTK KENLLLLRDL DKLHQKGYPI FLGVSRKRFV INILEENGFE 250
    VNPETELGFR NRDTASAHVT SIAARQGVEV VRVHDVASHR MAVEIASAIR 300
    LADEAENLDL KQYK 314
    Length:314
    Mass (Da):34,403
    Last modified:September 26, 2001 - v2
    Checksum:iF2F20D9A782083FB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti122 – 1232AY → PH in AAB63944. (PubMed:3114239)Curated
    Sequence conflicti131 – 1311K → Q in AAB63944. (PubMed:3114239)Curated
    Sequence conflicti159 – 1591T → A in AAB63944. (PubMed:3114239)Curated
    Sequence conflicti163 – 1631K → E in AAB63944. (PubMed:3114239)Curated
    Sequence conflicti175 – 1751D → E in AAB63944. (PubMed:3114239)Curated
    Sequence conflicti178 – 1781V → E in AAB63944. (PubMed:3114239)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti67 – 671E → EIE in mutant SUL-D.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16156 Genomic DNA. Translation: AAB63944.1.
    AE005672 Genomic DNA. Translation: AAK74467.1.
    PIRiA43661.
    B95034.
    B97905.
    RefSeqiNP_344827.1. NC_003028.3.

    Genome annotation databases

    EnsemblBacteriaiAAK74467; AAK74467; SP_0289.
    GeneIDi930100.
    KEGGispn:SP_0289.
    PATRICi19704915. VBIStrPne105772_0303.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U16156 Genomic DNA. Translation: AAB63944.1 .
    AE005672 Genomic DNA. Translation: AAK74467.1 .
    PIRi A43661.
    B95034.
    B97905.
    RefSeqi NP_344827.1. NC_003028.3.

    3D structure databases

    ProteinModelPortali P05382.
    SMRi P05382. Positions 7-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P05382. 3 interactions.
    STRINGi 170187.SP_0289.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK74467 ; AAK74467 ; SP_0289 .
    GeneIDi 930100.
    KEGGi spn:SP_0289.
    PATRICi 19704915. VBIStrPne105772_0303.

    Phylogenomic databases

    eggNOGi COG0294.
    HOGENOMi HOG000217509.
    KOi K00796.
    OMAi DMFYVAA.
    OrthoDBi EOG67T5P5.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00156 .
    BioCyci SPNE170187:GHGN-295-MONOMER.
    SABIO-RK P05382.

    Family and domain databases

    Gene3Di 3.20.20.20. 1 hit.
    InterProi IPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view ]
    Pfami PF00809. Pterin_bind. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51717. SSF51717. 1 hit.
    TIGRFAMsi TIGR01496. DHPS. 1 hit.
    PROSITEi PS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sulfonamide resistance in Streptococcus pneumoniae: DNA sequence of the gene encoding dihydropteroate synthase and characterization of the enzyme."
      Lopez P., Espinosa M., Greenberg B., Lacks S.A.
      J. Bacteriol. 169:4320-4326(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 772.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-334 / TIGR4.

    Entry informationi

    Entry nameiDHPS_STRPN
    AccessioniPrimary (citable) accession number: P05382
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The sequence shown here is that of sul-s (wild-type). The protein of the spontaneous mutation to sulfonamide resistance (sul-d) has an insert of 2 AA.

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3