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Protein

Phosphatidyl-N-methylethanolamine N-methyltransferase

Gene

OPI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the second two steps of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylmonomethylethanolamine (PMME) to phosphatidyldimethylethanolamine (PDME) and of PDME to phosphatidylcholine (PC). Can also catalyze the first methylation reaction of PE to PMME in the absence of PE methyltransferase CHO2.UniRule annotation8 Publications

Catalytic activityi

S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.3 Publications
S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine.UniRule annotation4 Publications
S-adenosyl-L-methionine + phosphatidyl-N-dimethylethanolamine = S-adenosyl-L-homocysteine + phosphatidylcholine.UniRule annotation4 Publications

Kineticsi

  1. KM=180 µM for S-adenosyl-L-methionine (in presence of phosphatidylethanolamine (PE) as substrate)1 Publication
  2. KM=190 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-methylethanolamine (PMME) as substrate)1 Publication
  3. KM=240 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-dimethylethanolamine (PDME) as substrate)1 Publication
  4. KM=270 µM for phosphatidyl-N-methylethanolamine (PMME)1 Publication
  5. KM=110 µM for phosphatidyl-N-dimethylethanolamine (PDME)1 Publication
  6. KM=54 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-methylethanolamine (PMME) as substrate)1 Publication
  7. KM=59 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-dimethylethanolamine (PDME) as substrate)1 Publication
  8. KM=380 µM for phosphatidyl-N-methylethanolamine (PMME)1 Publication
  9. KM=180 µM for phosphatidyl-N-dimethylethanolamine (PDME)1 Publication

    pH dependencei

    Optimum pH is 8.1.1 Publication

    Pathwayi: phosphatidylcholine biosynthesis

    This protein is involved in the pathway phosphatidylcholine biosynthesis, which is part of Phospholipid metabolism.UniRule annotation1 Publication
    View all proteins of this organism that are known to be involved in the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

    GO - Molecular functioni

    GO - Biological processi

    • phosphatidylcholine biosynthetic process Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:YJR073C-MONOMER.
    YEAST:YJR073C-MONOMER.
    ReactomeiR-SCE-1483191. Synthesis of PC.
    UniPathwayiUPA00753.

    Chemistry databases

    SwissLipidsiSLP:000000084.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidyl-N-methylethanolamine N-methyltransferaseUniRule annotationCurated (EC:2.1.1.173 Publications, EC:2.1.1.71UniRule annotation4 Publications)
    Alternative name(s):
    Overproducer of inositol protein 31 Publication
    Phospholipid methyltransferase2 PublicationsUniRule annotation
    Short name:
    PLMT2 PublicationsUniRule annotation
    Gene namesi
    Name:OPI31 Publication
    Synonyms:PEM21 Publication
    Ordered Locus Names:YJR073CImported
    ORF Names:J1824
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome X

    Organism-specific databases

    EuPathDBiFungiDB:YJR073C.
    SGDiS000003834. OPI3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 20LumenalUniRule annotationAdd BLAST20
    Intramembranei21 – 41HelicalUniRule annotationAdd BLAST21
    Topological domaini42 – 53LumenalUniRule annotationAdd BLAST12
    Transmembranei54 – 74HelicalUniRule annotationAdd BLAST21
    Topological domaini75 – 101CytoplasmicUniRule annotationAdd BLAST27
    Transmembranei102 – 122HelicalUniRule annotationAdd BLAST21
    Topological domaini123 – 165LumenalUniRule annotationAdd BLAST43
    Transmembranei166 – 186HelicalUniRule annotationAdd BLAST21
    Topological domaini187 – 206CytoplasmicUniRule annotationAdd BLAST20

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001939241 – 206Phosphatidyl-N-methylethanolamine N-methyltransferaseAdd BLAST206

    Proteomic databases

    PRIDEiP05375.

    Expressioni

    Inductioni

    Expression is repressed by inositol and choline. The 5' flanking region contains two copies of the CATRTGAA motif and a 5'-AAACCCACACATG-3' GRFI site, which are involved in the regulation of expression. OPI1 and SIN3 play the role of repressors for OPI3 expression whereas UME6 is an activator of OPI3 expression.4 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi33829. 575 interactors.
    DIPiDIP-2888N.
    IntActiP05375. 3 interactors.
    MINTiMINT-1650315.

    Structurei

    3D structure databases

    ProteinModelPortaliP05375.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni106 – 108S-adenosyl-L-methionine bindingUniRule annotation3
    Regioni188 – 189S-adenosyl-L-methionine bindingUniRule annotation2

    Sequence similaritiesi

    Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00390000007041.
    HOGENOMiHOG000208789.
    InParanoidiP05375.
    KOiK00550.
    OMAiTSTWALG.
    OrthoDBiEOG092C45C1.

    Family and domain databases

    HAMAPiMF_03216. PLMT. 1 hit.
    InterProiIPR024960. PEMT/MFAP.
    IPR007318. Phopholipid_MeTrfase.
    [Graphical view]
    PANTHERiPTHR15458. PTHR15458. 1 hit.
    PfamiPF04191. PEMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005444. PEMT. 1 hit.
    PROSITEiPS51599. SAM_PEMT_PEM2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05375-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKESVQEIIQ QLIHSVDLQS SKFQLAIVCT MFNPIFWNIV ARMEYHKHSL
    60 70 80 90 100
    TKMCGGARKG CYMLAATIFS LGIVRDMVYE SALREQPTCS LITGENWTKL
    110 120 130 140 150
    GVALFGLGQV LVLSSMYKLG ITGTYLGDYF GILMDERVTG FPFNVSNNPM
    160 170 180 190 200
    YQGSTLSFLG IALYKGKPAG LVVSAVVYFM YKIALRWEEP FTAMIYANRD

    KAKKNM
    Length:206
    Mass (Da):23,150
    Last modified:November 1, 1988 - v1
    Checksum:iBC0CDCC1C7B2AD36
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M16988 Genomic DNA. Translation: AAA34851.1.
    Z49573 Genomic DNA. Translation: CAA89601.1.
    L47993 Genomic DNA. Translation: AAB39298.1.
    AY557899 Genomic DNA. Translation: AAS56225.1.
    BK006943 Genomic DNA. Translation: DAA08859.1.
    PIRiB28443.
    RefSeqiNP_012607.1. NM_001181731.1.

    Genome annotation databases

    EnsemblFungiiYJR073C; YJR073C; YJR073C.
    GeneIDi853536.
    KEGGisce:YJR073C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M16988 Genomic DNA. Translation: AAA34851.1.
    Z49573 Genomic DNA. Translation: CAA89601.1.
    L47993 Genomic DNA. Translation: AAB39298.1.
    AY557899 Genomic DNA. Translation: AAS56225.1.
    BK006943 Genomic DNA. Translation: DAA08859.1.
    PIRiB28443.
    RefSeqiNP_012607.1. NM_001181731.1.

    3D structure databases

    ProteinModelPortaliP05375.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi33829. 575 interactors.
    DIPiDIP-2888N.
    IntActiP05375. 3 interactors.
    MINTiMINT-1650315.

    Chemistry databases

    SwissLipidsiSLP:000000084.

    Proteomic databases

    PRIDEiP05375.

    Protocols and materials databases

    DNASUi853536.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYJR073C; YJR073C; YJR073C.
    GeneIDi853536.
    KEGGisce:YJR073C.

    Organism-specific databases

    EuPathDBiFungiDB:YJR073C.
    SGDiS000003834. OPI3.

    Phylogenomic databases

    GeneTreeiENSGT00390000007041.
    HOGENOMiHOG000208789.
    InParanoidiP05375.
    KOiK00550.
    OMAiTSTWALG.
    OrthoDBiEOG092C45C1.

    Enzyme and pathway databases

    UniPathwayiUPA00753.
    BioCyciMetaCyc:YJR073C-MONOMER.
    YEAST:YJR073C-MONOMER.
    ReactomeiR-SCE-1483191. Synthesis of PC.

    Miscellaneous databases

    PROiP05375.

    Family and domain databases

    HAMAPiMF_03216. PLMT. 1 hit.
    InterProiIPR024960. PEMT/MFAP.
    IPR007318. Phopholipid_MeTrfase.
    [Graphical view]
    PANTHERiPTHR15458. PTHR15458. 1 hit.
    PfamiPF04191. PEMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005444. PEMT. 1 hit.
    PROSITEiPS51599. SAM_PEMT_PEM2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPLMT_YEAST
    AccessioniPrimary (citable) accession number: P05375
    Secondary accession number(s): D6VWP3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: November 30, 2016
    This is version 140 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5890 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.