P05375 (PEM2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylene-fatty-acyl-phospholipid synthase
EC=2.1.1.16

Alternative name(s):
Overproducer of inositol protein 3
Unsaturated phospholipid methyltransferase

Gene names

Name:	OPI3
Synonyms:	PEM2
Ordered Locus Names:	YJR073C
ORF Names:	J1824

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid methylene fatty acid.
Pathway	Phospholipid metabolism; phosphatidylcholine biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Miscellaneous	Present with 5890 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the PEMT/PEM2 methyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphatidylcholine biosynthetic process Inferred from direct assay Ref.1. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD
Molecular function	methylene-fatty-acyl-phospholipid synthase activity Inferred from electronic annotation. Source: EC phosphatidyl-N-dimethylethanolamine N-methyltransferase activity Inferred from direct assay Ref.1. Source: SGD phosphatidyl-N-methylethanolamine N-methyltransferase activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Methylene-fatty-acyl-phospholipid synthase		PRO_0000193924
Regions
	Transmembrane	25 – 41	17	Helical; Potential
	Transmembrane	100 – 117	18	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P05375 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: BC0CDCC1C7B2AD36	FASTA	206	23,150
	10 20 30 40 50 60 MKESVQEIIQ QLIHSVDLQS SKFQLAIVCT MFNPIFWNIV ARMEYHKHSL TKMCGGARKG 70 80 90 100 110 120 CYMLAATIFS LGIVRDMVYE SALREQPTCS LITGENWTKL GVALFGLGQV LVLSSMYKLG 130 140 150 160 170 180 ITGTYLGDYF GILMDERVTG FPFNVSNNPM YQGSTLSFLG IALYKGKPAG LVVSAVVYFM 190 200 YKIALRWEEP FTAMIYANRD KAKKNM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes." Kodaki T., Yamashita S. J. Biol. Chem. 262:15428-15435(1987) [PubMed: 2445736] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed: 8840504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M16988 Genomic DNA. Translation: AAA34851.1. Z49573 Genomic DNA. Translation: CAA89601.1. L47993 Genomic DNA. Translation: AAB39298.1. AY557899 Genomic DNA. Translation: AAS56225.1. BK006943 Genomic DNA. Translation: DAA08859.1.
PIR	B28443.
RefSeq	NP_012607.1. NM_001181731.1.
3D structure databases
ProteinModelPortal	P05375.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2888N.
IntAct	P05375. 3 interactions.
STRING	P05375.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR073C; YJR073C; YJR073C.
GeneID	853536.
KEGG	sce:YJR073C.
NMPDR	fig|4932.3.peg.3581.
Organism-specific databases
CYGD	YJR073c.
SGD	S000003834. OPI3.
Phylogenomic databases
eggNOG	fuNOG09615.
GeneTree	EFGT00050000006199.
HOGENOM	HBG524237.
OMA	VVARWEH.
OrthoDB	EOG4SFDG8.
Gene expression databases
ArrayExpress	P05375.
Genevestigator	P05375.
GermOnline	YJR073C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007318. PEMT. [Graphical view]
KO	K00550.
PANTHER	PTHR15458. PEMT. 1 hit.
Pfam	PF04191. PEMT. 1 hit. [Graphical view]
PIRSF	PIRSF005444. PEMT. 1 hit.
ProtoNet	Search...
Other
NextBio	974242.

Entry information

Entry name

PEM2_YEAST

Accession

Primary (citable) accession number: P05375
Secondary accession number(s): D6VWP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	December 14, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program