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Protein

Phosphatidyl-N-methylethanolamine N-methyltransferase

Gene

OPI3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC).PROSITE-ProRule annotation8 Publications

Catalytic activityi

S-adenosyl-L-methionine + phosphatidyl-N-methylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-dimethylethanolamine.PROSITE-ProRule annotation
S-adenosyl-L-methionine + phosphatidyl-N-dimethylethanolamine = S-adenosyl-L-homocysteine + phosphatidylcholine.PROSITE-ProRule annotation
S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.PROSITE-ProRule annotation

Kineticsi

  1. KM=54 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-methylethanolamine (PMME) as substrate)2 Publications
  2. KM=59 µM for S-adenosyl-L-methionine (in presence of phosphatidyl-N-dimethylethanolamine (PDME) as substrate)2 Publications
  3. KM=380 µM for phosphatidyl-N-methylethanolamine (PMME)2 Publications
  4. KM=180 µM for phosphatidyl-N-dimethylethanolamine (PDME)2 Publications

    pH dependencei

    Optimum pH is 8.1.2 Publications

    Pathwayi: phosphatidylcholine biosynthesis

    This protein is involved in the pathway phosphatidylcholine biosynthesis, which is part of Phospholipid metabolism.PROSITE-ProRule annotation
    View all proteins of this organism that are known to be involved in the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

    GO - Molecular functioni

    GO - Biological processi

    • phosphatidylcholine biosynthetic process Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16681.
    YEAST:YJR073C-MONOMER.
    ReactomeiR-SCE-1483191. Synthesis of PC.
    UniPathwayiUPA00753.

    Chemistry

    SwissLipidsiSLP:000000084.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidyl-N-methylethanolamine N-methyltransferase (EC:2.1.1.17, EC:2.1.1.71)
    Short name:
    PLMT
    Alternative name(s):
    Overproducer of inositol protein 3
    Unsaturated phospholipid methyltransferase
    Gene namesi
    Name:OPI3
    Synonyms:PEM2
    Ordered Locus Names:YJR073C
    ORF Names:J1824
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome X

    Organism-specific databases

    EuPathDBiFungiDB:YJR073C.
    SGDiS000003834. OPI3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4117HelicalSequence analysisAdd
    BLAST
    Transmembranei62 – 7918HelicalSequence analysisAdd
    BLAST
    Transmembranei100 – 11718HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 206206Phosphatidyl-N-methylethanolamine N-methyltransferasePRO_0000193924Add
    BLAST

    Expressioni

    Inductioni

    Expression is repressed by inositol and choline. The 5' flanking region contains two copies of the CATRTGAA motif and a 5'-AAACCCACACATG-3' GRFI site, which are involved in the regulation of expression. OPI1 and SIN3 play the role of repressors for OPI3 expression whereas UME6 is an activator of OPI3 expression.4 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi33829. 575 interactions.
    DIPiDIP-2888N.
    IntActiP05375. 3 interactions.
    MINTiMINT-1650315.

    Structurei

    3D structure databases

    ProteinModelPortaliP05375.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00390000007041.
    HOGENOMiHOG000208789.
    InParanoidiP05375.
    KOiK00550.
    OMAiTSTWALG.
    OrthoDBiEOG71K6FR.

    Family and domain databases

    InterProiIPR024960. PEMT/MFAP.
    IPR007318. Phopholipid_MeTrfase.
    [Graphical view]
    PANTHERiPTHR15458. PTHR15458. 1 hit.
    PfamiPF04191. PEMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005444. PEMT. 1 hit.
    PROSITEiPS51599. SAM_PEMT_PEM2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05375-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKESVQEIIQ QLIHSVDLQS SKFQLAIVCT MFNPIFWNIV ARMEYHKHSL
    60 70 80 90 100
    TKMCGGARKG CYMLAATIFS LGIVRDMVYE SALREQPTCS LITGENWTKL
    110 120 130 140 150
    GVALFGLGQV LVLSSMYKLG ITGTYLGDYF GILMDERVTG FPFNVSNNPM
    160 170 180 190 200
    YQGSTLSFLG IALYKGKPAG LVVSAVVYFM YKIALRWEEP FTAMIYANRD

    KAKKNM
    Length:206
    Mass (Da):23,150
    Last modified:November 1, 1988 - v1
    Checksum:iBC0CDCC1C7B2AD36
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M16988 Genomic DNA. Translation: AAA34851.1.
    Z49573 Genomic DNA. Translation: CAA89601.1.
    L47993 Genomic DNA. Translation: AAB39298.1.
    AY557899 Genomic DNA. Translation: AAS56225.1.
    BK006943 Genomic DNA. Translation: DAA08859.1.
    PIRiB28443.
    RefSeqiNP_012607.1. NM_001181731.1.

    Genome annotation databases

    EnsemblFungiiYJR073C; YJR073C; YJR073C.
    GeneIDi853536.
    KEGGisce:YJR073C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M16988 Genomic DNA. Translation: AAA34851.1.
    Z49573 Genomic DNA. Translation: CAA89601.1.
    L47993 Genomic DNA. Translation: AAB39298.1.
    AY557899 Genomic DNA. Translation: AAS56225.1.
    BK006943 Genomic DNA. Translation: DAA08859.1.
    PIRiB28443.
    RefSeqiNP_012607.1. NM_001181731.1.

    3D structure databases

    ProteinModelPortaliP05375.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi33829. 575 interactions.
    DIPiDIP-2888N.
    IntActiP05375. 3 interactions.
    MINTiMINT-1650315.

    Chemistry

    SwissLipidsiSLP:000000084.

    Protocols and materials databases

    DNASUi853536.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYJR073C; YJR073C; YJR073C.
    GeneIDi853536.
    KEGGisce:YJR073C.

    Organism-specific databases

    EuPathDBiFungiDB:YJR073C.
    SGDiS000003834. OPI3.

    Phylogenomic databases

    GeneTreeiENSGT00390000007041.
    HOGENOMiHOG000208789.
    InParanoidiP05375.
    KOiK00550.
    OMAiTSTWALG.
    OrthoDBiEOG71K6FR.

    Enzyme and pathway databases

    UniPathwayiUPA00753.
    BioCyciMetaCyc:MONOMER-16681.
    YEAST:YJR073C-MONOMER.
    ReactomeiR-SCE-1483191. Synthesis of PC.

    Miscellaneous databases

    PROiP05375.

    Family and domain databases

    InterProiIPR024960. PEMT/MFAP.
    IPR007318. Phopholipid_MeTrfase.
    [Graphical view]
    PANTHERiPTHR15458. PTHR15458. 1 hit.
    PfamiPF04191. PEMT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005444. PEMT. 1 hit.
    PROSITEiPS51599. SAM_PEMT_PEM2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes."
      Kodaki T., Yamashita S.
      J. Biol. Chem. 262:15428-15435(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
      Huang M.-E., Manus V., Chuat J.-C., Galibert F.
      Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Regulation of the phosphatidylethanolamine methylation pathway in Saccharomyces cerevisiae."
      Yamashita S., Oshima A., Nikawa J., Hosaka K.
      Eur. J. Biochem. 128:589-595(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, INDUCTION.
    7. "Regulatory mutations of inositol biosynthesis in yeast: isolation of inositol-excreting mutants."
      Greenberg M.L., Reiner B., Henry S.A.
      Genetics 100:19-33(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Yeast mutant defective in phosphatidylcholine synthesis."
      Greenberg M.L., Klig L.S., Letts V.A., Loewy B.S., Henry S.A.
      J. Bacteriol. 153:791-799(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Inositol regulates phosphatidylglycerolphosphate synthase expression in Saccharomyces cerevisiae."
      Greenberg M.L., Hubbell S., Lam C.
      Mol. Cell. Biol. 8:4773-4779(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Characterization of the methyltransferases in the yeast phosphatidylethanolamine methylation pathway by selective gene disruption."
      Kodaki T., Yamashita S.
      Eur. J. Biochem. 185:243-251(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, FUNCTION.
    11. "Mutations in the Saccharomyces cerevisiae opi3 gene: effects on phospholipid methylation, growth and cross-pathway regulation of inositol synthesis."
      McGraw P., Henry S.A.
      Genetics 122:317-330(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Phosphatidylethanolamine methyltransferase and phospholipid methyltransferase activities from Saccharomyces cerevisiae. Enzymological and kinetic properties."
      Gaynor P.M., Carman G.M.
      Biochim. Biophys. Acta 1045:156-163(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, FUNCTION.
    13. "Regulation of phosphatidylethanolamine methyltransferase and phospholipid methyltransferase by phospholipid precursors in Saccharomyces cerevisiae."
      Gaynor P.M., Gill T., Toutenhoofd S., Summers E.F., McGraw P., Homann M.J., Henry S.A., Carman G.M.
      Biochim. Biophys. Acta 1090:326-332(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    14. "Identification of the upstream activation sequences responsible for the expression and regulation of the PEM1 and PEM2 genes encoding the enzymes of the phosphatidylethanolamine methylation pathway in Saccharomyces cerevisiae."
      Kodaki T., Hosaka K., Nikawa J., Yamashita S.
      J. Biochem. 109:276-287(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    15. "The yeast UME6 gene is required for both negative and positive transcriptional regulation of phospholipid biosynthetic gene expression."
      Jackson J.C., Lopes J.M.
      Nucleic Acids Res. 24:1322-1329(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    16. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    17. "Quantitative profiling of PE, MMPE, DMPE, and PC lipid species by multiple precursor ion scanning: a tool for monitoring PE metabolism."
      Bilgin M., Markgraf D.F., Duchoslav E., Knudsen J., Jensen O.N., de Kroon A.I., Ejsing C.S.
      Biochim. Biophys. Acta 1811:1081-1089(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPEM2_YEAST
    AccessioniPrimary (citable) accession number: P05375
    Secondary accession number(s): D6VWP3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: July 6, 2016
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5890 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.