Skip Header

 
Contribute Send feedback
Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot P05374 (PEM1_YEAST)

Last modified June 16, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Phosphatidylethanolamine N-methyltransferase
      Short name=PEAMT
    EC=2.1.1.17
Gene names
Name: PEM1
Synonyms: CHO2
Ordered Locus Names: YGR157W
ORF Names: G6673
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length869 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Catalytic activity

S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.

Pathway

Phospholipid metabolism; phosphatidylcholine biosynthesis.

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

Present with 1810 molecules/cell in log phase SD medium. Ref.4

Hide | Top

Ontologies

Keywords
   Biological processPhospholipid biosynthesis
   Cellular componentMembrane
   DomainTransmembrane
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processphosphatidylcholine biosynthetic process Ref.1

Inferred from direct assay. Source: SGD

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionphosphatidylethanolamine N-methyltransferase activity Ref.1

Inferred from direct assay. Source: SGD

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 869869Phosphatidylethanolamine N-methyltransferase
PRO_0000058304

Regions

Topological domain1 – 5959Cytoplasmic Potential
Transmembrane60 – 8021 Potential
Topological domain81 – 844Extracellular Potential
Transmembrane85 – 10521 Potential
Topological domain106 – 17873Cytoplasmic Potential
Transmembrane179 – 19921 Potential
Topological domain200 – 21213Extracellular Potential
Transmembrane213 – 23321 Potential
Topological domain234 – 24411Cytoplasmic Potential
Transmembrane245 – 26521 Potential
Topological domain266 – 28520Extracellular Potential
Transmembrane286 – 30621 Potential
Topological domain307 – 36559Cytoplasmic Potential
Transmembrane366 – 38621 Potential
Topological domain387 – 3904Extracellular Potential
Transmembrane391 – 41121 Potential
Topological domain412 – 43827Cytoplasmic Potential
Transmembrane439 – 45921 Potential
Topological domain460 – 4634Extracellular Potential
Transmembrane464 – 48421 Potential
Topological domain485 – 49511Cytoplasmic Potential
Transmembrane496 – 51621 Potential
Topological domain517 – 54226Extracellular Potential
Transmembrane543 – 56321 Potential
Topological domain564 – 869306Cytoplasmic Potential

Amino acid modifications

Modified residue5821Phosphoserine Ref.6

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P05374-1 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: A273F179B4E46A20

FASTA869101,204
        10         20         30         40         50         60 
MSSCKTTLSE MVGSVTKDRG TINVEARTRS SNVTFKPPVT HDMVRSLFDP TLKKSLLEKC 

        70         80         90        100        110        120 
IALAIISNFF ICYWVFQRFG LQFTKYFFLV QYLFWRIAYN LGIGLVLHYQ SHYETLTNCA 

       130        140        150        160        170        180 
KTHAIFSKIP QNKDANSNFS TNSNSFSEKF WNFIRKFCQY EIRSKMPKEY DLFAYPEEIN 

       190        200        210        220        230        240 
VWLIFRQFVD LILMQDFVTY IIYVYLSIPY SWVQIFNWRS LLGVILILFN IWVKLDAHRV 

       250        260        270        280        290        300 
VKDYAWYWGD FFFLEESELI FDGVFNISPH PMYSIGYLGY YGLSLICNDY KVLLVSVFGH 

       310        320        330        340        350        360 
YSQFLFLKYV ENPHIERTYG DGTDSDSQMN SRIDDLISKE NYDYSRPLIN MGLSFNNFNK 

       370        380        390        400        410        420 
LRFTDYFTIG TVAALMLGTI MNARFINLNY LFITVFVTKL VSWLFISTIL YKQSQSKWFT 

       430        440        450        460        470        480 
RLFLENGYTQ VYSYEQWQFI YNYYLVLTYT LMIIHTGLQI WSNFSNINNS QLIFGLILVA 

       490        500        510        520        530        540 
LQTWCDKETR LAISDFGWFY GDFFLSNYIS TRKLTSQGIY RYLNHPEAVL GVVGVWGTVL 

       550        560        570        580        590        600 
MTNFAVTNII LAVLWTLTNF ILVKFIETPH VNKIYGKTKR VSGVGKTLLG LKPLRQVSDI 

       610        620        630        640        650        660 
VNRIENIIIK SLVDESKNSN GGAELLPKNY QDNKEWNILI QEAMDSVATR LSPYCELKIE 

       670        680        690        700        710        720 
NEQVETNFVL PTPVTLNWKM PIELYNGDDW IGLYKVIDTR ADREKTRVGS GGHWSATSKD 

       730        740        750        760        770        780 
SYMNHGLRHK ESVTEIKATE KYVQGKVTFD TSLLYFENGI YEFRYHSGNS HKVLLISTPF 

       790        800        810        820        830        840 
EISLPVLNTT TPELFEKDLT EFLTKVNVLK DGKFRPLGNK FFGMDSLKQL IKNSIGVELS 

       850        860 
SEYMRRVNGD AHVISHRAWD IKQTLDSLA

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes."
Kodaki T., Yamashita S.
J. Biol. Chem. 262:15428-15435(1987) [PubMed: 2445736] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames."
Skala J., Nawrocki A., Goffeau A.
Yeast 11:1421-1427(1995) [PubMed: 8585325] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
[6]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-582, MASS SPECTROMETRY.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

M16987 Genomic DNA. Translation: AAA34850.1.
X85807 Genomic DNA. Translation: CAA59814.1.
Z72942 Genomic DNA. Translation: CAA97171.1.
PIRA28443.
RefSeqNP_011673.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:8037N.
IntActP05374. 13 interactions.

Proteomic databases

PeptideAtlasP05374.

Genome annotation databases

EnsemblYGR157W. Saccharomyces cerevisiae. [Contig view]
GeneID853061.
GenomeReviewsGene locus YGR157W in contig Y13135_GR.
KEGGsce:YGR157W.
NMPDRfig|4932.3.peg.2793.

Organism-specific databases

CYGDYGR157w.
SGDS000003389. CHO2.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP05374.
OMAP05374. APHPMYS.

Enzyme and pathway databases

BRENDA2.1.1.17. 250.

Gene expression databases

ArrayExpressP05374.
GermOnlineYGR157W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR016219. Phosphatid-EA_MeTrfase_fun.
[Graphical view]
PIRSFPIRSF000383. PEAMT. 1 hit.
ProtoNetSearch...

Other Resources

NextBio972998.

Hide | Top

Entry information

Entry namePEM1_YEAST
AccessionPrimary (citable) accession number: P05374
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents