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Protein

Phosphatidylethanolamine N-methyltransferase

Gene

CHO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first step of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME). Preferentially converts di-C16:1 substrates.UniRule annotation8 Publications

Miscellaneous

Present with 1810 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + phosphatidylethanolamine = S-adenosyl-L-homocysteine + phosphatidyl-N-methylethanolamine.UniRule annotation4 Publications

Kineticsi

  1. KM=60 µM for S-adenosyl-L-methionine1 Publication
  2. KM=110 µM for S-adenosyl-L-methionine1 Publication
  3. KM=57 µM for phosphatidylethanolamine (PE)1 Publication

    pH dependencei

    Optimum pH is 9.9.1 Publication

    Pathwayi: phosphatidylcholine biosynthesis

    This protein is involved in the pathway phosphatidylcholine biosynthesis, which is part of Phospholipid metabolism.UniRule annotation2 Publications
    View all proteins of this organism that are known to be involved in the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

    GO - Molecular functioni

    • phosphatidylethanolamine N-methyltransferase activity Source: SGD

    GO - Biological processi

    • phosphatidylcholine biosynthetic process Source: SGD

    Keywordsi

    Molecular functionMethyltransferase, Transferase
    Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
    LigandS-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:YGR157W-MONOMER
    YEAST:YGR157W-MONOMER
    UniPathwayiUPA00753

    Chemistry databases

    SwissLipidsiSLP:000000085

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylethanolamine N-methyltransferase1 PublicationUniRule annotation (EC:2.1.1.17UniRule annotation4 Publications)
    Short name:
    PE methyltransferase1 PublicationUniRule annotation
    Short name:
    PEAMTUniRule annotation
    Short name:
    PEMT1 PublicationUniRule annotation
    Alternative name(s):
    Choline-requiring protein 21 Publication
    Gene namesi
    Name:CHO21 Publication
    Synonyms:PEM11 Publication
    Ordered Locus Names:YGR157WImported
    ORF Names:G6673
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome VII

    Organism-specific databases

    EuPathDBiFungiDB:YGR157W
    SGDiS000003389 CHO2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini2 – 55LumenalUniRule annotation1 PublicationAdd BLAST54
    Transmembranei56 – 76HelicalUniRule annotationAdd BLAST21
    Topological domaini77 – 86CytoplasmicUniRule annotation1 Publication10
    Transmembranei87 – 107HelicalUniRule annotationAdd BLAST21
    Topological domaini108 – 187LumenalUniRule annotation1 PublicationAdd BLAST80
    Transmembranei188 – 208HelicalUniRule annotationAdd BLAST21
    Topological domaini209 – 212CytoplasmicUniRule annotation1 Publication4
    Transmembranei213 – 233HelicalUniRule annotationAdd BLAST21
    Topological domaini234 – 258LumenalUniRule annotation1 PublicationAdd BLAST25
    Transmembranei259 – 279HelicalUniRule annotationAdd BLAST21
    Topological domaini280 – 291CytoplasmicUniRule annotation1 PublicationAdd BLAST12
    Transmembranei292 – 310HelicalUniRule annotationAdd BLAST19
    Topological domaini311 – 362LumenalUniRule annotation1 PublicationAdd BLAST52
    Transmembranei363 – 383HelicalUniRule annotationAdd BLAST21
    Topological domaini384 – 389CytoplasmicUniRule annotation1 Publication6
    Transmembranei390 – 410HelicalUniRule annotationAdd BLAST21
    Topological domaini411 – 439LumenalUniRule annotation1 PublicationAdd BLAST29
    Transmembranei440 – 460HelicalUniRule annotationAdd BLAST21
    Topological domaini461 – 463CytoplasmicUniRule annotation1 Publication3
    Transmembranei464 – 484HelicalUniRule annotationAdd BLAST21
    Topological domaini485 – 534LumenalUniRule annotation1 PublicationAdd BLAST50
    Transmembranei535 – 555HelicalUniRule annotationAdd BLAST21
    Topological domaini556 – 869CytoplasmicUniRule annotation1 PublicationAdd BLAST314

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources
    ChainiPRO_00000583042 – 869Phosphatidylethanolamine N-methyltransferaseAdd BLAST868

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserineCombined sources1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP05374
    PaxDbiP05374
    PRIDEiP05374

    PTM databases

    iPTMnetiP05374

    Expressioni

    Inductioni

    Repressed by myo-inositol and choline.4 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi33405, 638 interactors
    DIPiDIP-8037N
    IntActiP05374, 3 interactors
    MINTiP05374
    STRINGi4932.YGR157W

    Structurei

    3D structure databases

    ProteinModelPortaliP05374
    SMRiP05374
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    InParanoidiP05374
    KOiK16369
    OMAiEAWRQWK
    OrthoDBiEOG092C0F70

    Family and domain databases

    HAMAPiMF_03217 PEMT, 1 hit
    InterProiView protein in InterPro
    IPR007318 Phopholipid_MeTrfase
    IPR016219 Phosphatid-EA_MeTrfase_fun
    PfamiView protein in Pfam
    PF04191 PEMT, 2 hits
    PIRSFiPIRSF000383 PEAMT, 1 hit
    PROSITEiView protein in PROSITE
    PS51598 SAM_CHO2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05374-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSCKTTLSE MVGSVTKDRG TINVEARTRS SNVTFKPPVT HDMVRSLFDP
    60 70 80 90 100
    TLKKSLLEKC IALAIISNFF ICYWVFQRFG LQFTKYFFLV QYLFWRIAYN
    110 120 130 140 150
    LGIGLVLHYQ SHYETLTNCA KTHAIFSKIP QNKDANSNFS TNSNSFSEKF
    160 170 180 190 200
    WNFIRKFCQY EIRSKMPKEY DLFAYPEEIN VWLIFRQFVD LILMQDFVTY
    210 220 230 240 250
    IIYVYLSIPY SWVQIFNWRS LLGVILILFN IWVKLDAHRV VKDYAWYWGD
    260 270 280 290 300
    FFFLEESELI FDGVFNISPH PMYSIGYLGY YGLSLICNDY KVLLVSVFGH
    310 320 330 340 350
    YSQFLFLKYV ENPHIERTYG DGTDSDSQMN SRIDDLISKE NYDYSRPLIN
    360 370 380 390 400
    MGLSFNNFNK LRFTDYFTIG TVAALMLGTI MNARFINLNY LFITVFVTKL
    410 420 430 440 450
    VSWLFISTIL YKQSQSKWFT RLFLENGYTQ VYSYEQWQFI YNYYLVLTYT
    460 470 480 490 500
    LMIIHTGLQI WSNFSNINNS QLIFGLILVA LQTWCDKETR LAISDFGWFY
    510 520 530 540 550
    GDFFLSNYIS TRKLTSQGIY RYLNHPEAVL GVVGVWGTVL MTNFAVTNII
    560 570 580 590 600
    LAVLWTLTNF ILVKFIETPH VNKIYGKTKR VSGVGKTLLG LKPLRQVSDI
    610 620 630 640 650
    VNRIENIIIK SLVDESKNSN GGAELLPKNY QDNKEWNILI QEAMDSVATR
    660 670 680 690 700
    LSPYCELKIE NEQVETNFVL PTPVTLNWKM PIELYNGDDW IGLYKVIDTR
    710 720 730 740 750
    ADREKTRVGS GGHWSATSKD SYMNHGLRHK ESVTEIKATE KYVQGKVTFD
    760 770 780 790 800
    TSLLYFENGI YEFRYHSGNS HKVLLISTPF EISLPVLNTT TPELFEKDLT
    810 820 830 840 850
    EFLTKVNVLK DGKFRPLGNK FFGMDSLKQL IKNSIGVELS SEYMRRVNGD
    860
    AHVISHRAWD IKQTLDSLA
    Length:869
    Mass (Da):101,204
    Last modified:November 1, 1988 - v1
    Checksum:iA273F179B4E46A20
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M16987 Genomic DNA Translation: AAA34850.1
    X85807 Genomic DNA Translation: CAA59814.1
    Z72942 Genomic DNA Translation: CAA97171.1
    BK006941 Genomic DNA Translation: DAA08248.1
    PIRiA28443
    RefSeqiNP_011673.1, NM_001181286.1

    Genome annotation databases

    EnsemblFungiiYGR157W; YGR157W; YGR157W
    GeneIDi853061
    KEGGisce:YGR157W

    Similar proteinsi

    Entry informationi

    Entry nameiCHO2_YEAST
    AccessioniPrimary (citable) accession number: P05374
    Secondary accession number(s): D6VUT7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: March 28, 2018
    This is version 145 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health