Glucose-6-phosphate 1-dehydrogenase - Rattus norvegicus (Rat)

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Reviewed, UniProtKB/Swiss-Prot P05370 (G6PD_RAT)

Last modified February 9, 2010. Version 98. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase
      Short name=G6PD
    EC=1.1.1.49

Gene names

Name:	G6pdx
Synonyms:	G6pd

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP⁺ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Miscellaneous	Has NADP both as cofactor (bound to the N-terminal domain) and as structural element bound to the C-terminal domain.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

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Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glucose 6-phosphate metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt, oxidative branch Inferred from mutant phenotype. Source: RGD regulation of anti-apoptosis Inferred from direct assay. Source: RGD response to ethanol Inferred from direct assay. Source: RGD response to food Inferred from expression pattern. Source: RGD response to organic cyclic substance Inferred from expression pattern. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD microsome Inferred from direct assay. Source: RGD nucleus Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	NADP or NADPH binding Inferred from direct assay. Source: RGD glucose binding Inferred from direct assay. Source: RGD glucose-6-phosphate dehydrogenase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.3

Chain

2 – 515

514

Glucose-6-phosphate 1-dehydrogenase

PRO_0000068087

Sites

Active site

263

Proton acceptor By similarity

Binding site

NADP By similarity

Binding site

NADP By similarity

Binding site

201

Substrate By similarity

Binding site

205

Substrate By similarity

Binding site

238

NADP By similarity

Binding site

357

NADP By similarity

Binding site

487

NADP By similarity

Amino acid modifications

Modified residue

N-acetylalanine Ref.3

Modified residue

N6-acetyllysine By similarity

Modified residue

171

N6-acetyllysine By similarity

Modified residue

386

N6-acetyllysine By similarity

Modified residue

401

Phosphotyrosine By similarity

Modified residue

403

N6-acetyllysine By similarity

Modified residue

432

N6-acetyllysine By similarity

Modified residue

497

N6-acetyllysine By similarity

Modified residue

503

Phosphotyrosine By similarity

Modified residue

507

Phosphotyrosine By similarity

Modified residue

514

N6-acetyllysine By similarity

Experimental info

Sequence conflict

320

N → D AA sequence Ref.3

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Sequences

Sequence

Length

Mass (Da)

Tools

P05370-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8E92FFF3BF5A959B
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FASTA

515

59,376

        10         20         30         40         50         60 
MAEQVALSRT QVCGILREEL YQGDAFHQAD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL 

        70         80         90        100        110        120 
LPEDTFIVGY ARSRLTVDDI RKQSEPFFKV TPEERPKLEE FFARNSYVAG QYDDPASYKH 

       130        140        150        160        170        180 
LNSHMNALHQ GMQANRLFYL ALPPTVYEAV TKNIQEICMS QTGWNRIIVE KPFGRDLQSS 

       190        200        210        220        230        240 
NQLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP 

       250        260        270        280        290        300 
FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTD SDDVRDEKVK VLKCISEVET 

       310        320        330        340        350        360 
DNVVLGQYVG NPSGEGEATN GYLDDPTVPH GSTTATFAAA VLYVENERWD GVPFILRCGK 

       370        380        390        400        410        420 
ALNERKAEVR LQFRDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL 

       430        440        450        460        470        480 
DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH KIDREKPQPI 

       490        500        510 
PYVYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence of a cDNA encoding rat glucose-6-phosphate dehydrogenase." Ho Y., Howard A.J., Crapo J.D. Nucleic Acids Res. 16:7746-7746(1988) [PubMed: 3412913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Glucose-6-phosphate dehydrogenase. Characteristics revealed by the rat liver enzyme structure." Jeffery J., Barros-Soederling J., Murray L., Wood I., Hansen R., Szepesi B., Joernvall H. Eur. J. Biochem. 186:551-556(1989) [PubMed: 2606104] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-514. Strain: Wistar. Tissue: Liver.
[4]	Fritz R.S., Kletzien R.F. Submitted (NOV-1989) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 41-515.
[5]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 58-72; 176-192 AND 228-257, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
EMBL GenBank DDBJ	X07467 mRNA. Translation: CAA30355.1. BC081820 mRNA. Translation: AAH81820.1. M26655, M26653, M26654 Genomic DNA. Translation: AAA41179.1.
IPI	IPI00231637.
PIR	S01233.
RefSeq	NP_058702.1.
UniGene	Rn.11040
3D structure databases
SMR	P05370. Positions 28-515.
ModBase	Search...
Protein-protein interaction databases
STRING	P05370.
PTM databases
PhosphoSite	P05370.
Proteomic databases
PRIDE	P05370.
Genome annotation databases
Ensembl	ENSRNOT00000056317; ENSRNOP00000053157; ENSRNOG00000037254; Rattus norvegicus. [Genome view]
GeneID	24377.
KEGG	rno:24377.
Organism-specific databases
CTD	24377.
RGD	2645. G6pdx.
Phylogenomic databases
eggNOG	maNOG04087.
HOVERGEN	P05370.
InParanoid	P05370.
PhylomeDB	P05370.
Enzyme and pathway databases
BRENDA	1.1.1.49. 248.
Gene expression databases
ArrayExpress	P05370.
Genevestigator	P05370.
GermOnline	ENSRNOG00000037254. Rattus norvegicus.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603131.

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Entry information

Entry name

G6PD_RAT

Accession

Primary (citable) accession number: P05370

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 98 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents