Reviewed, UniProtKB/Swiss-Prot P05370 (G6PD_RAT)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase
      Short name=G6PD
    EC=1.1.1.49

Gene names

Name:	G6pdx
Synonyms:	G6pd

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Miscellaneous	Has NADP both as cofactor (bound to the N-terminal domain) and as structural element bound to the C-terminal domain.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glucose 6-phosphate metabolic process Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt, oxidative branch Inferred from mutant phenotype. Source: RGD regulation of anti-apoptosis Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD microsome Inferred from direct assay. Source: RGD nucleus Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	NADP or NADPH binding Inferred from direct assay. Source: RGD glucose binding Inferred from direct assay. Source: RGD glucose-6-phosphate dehydrogenase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 515	514	Glucose-6-phosphate 1-dehydrogenase		PRO_0000068087
Sites
	Active site	263	1	Proton acceptor By similarity
	Binding site	40	1	NADP By similarity
	Binding site	72	1	NADP By similarity
	Binding site	201	1	Substrate By similarity
	Binding site	205	1	Substrate By similarity
	Binding site	238	1	NADP By similarity
	Binding site	357	1	NADP By similarity
	Binding site	487	1	NADP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.3
	Modified residue	401	1	Phosphotyrosine By similarity
	Modified residue	503	1	Phosphotyrosine By similarity
	Modified residue	507	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	320	1	N → D AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P05370-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8E92FFF3BF5A959B	FASTA	515	59,376
	10 20 30 40 50 60 MAEQVALSRT QVCGILREEL YQGDAFHQAD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL 70 80 90 100 110 120 LPEDTFIVGY ARSRLTVDDI RKQSEPFFKV TPEERPKLEE FFARNSYVAG QYDDPASYKH 130 140 150 160 170 180 LNSHMNALHQ GMQANRLFYL ALPPTVYEAV TKNIQEICMS QTGWNRIIVE KPFGRDLQSS 190 200 210 220 230 240 NQLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP 250 260 270 280 290 300 FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTD SDDVRDEKVK VLKCISEVET 310 320 330 340 350 360 DNVVLGQYVG NPSGEGEATN GYLDDPTVPH GSTTATFAAA VLYVENERWD GVPFILRCGK 370 380 390 400 410 420 ALNERKAEVR LQFRDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL 430 440 450 460 470 480 DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH KIDREKPQPI 490 500 510 PYVYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence of a cDNA encoding rat glucose-6-phosphate dehydrogenase." Ho Y., Howard A.J., Crapo J.D. Nucleic Acids Res. 16:7746-7746(1988) [PubMed: 3412913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Glucose-6-phosphate dehydrogenase. Characteristics revealed by the rat liver enzyme structure." Jeffery J., Barros-Soederling J., Murray L., Wood I., Hansen R., Szepesi B., Joernvall H. Eur. J. Biochem. 186:551-556(1989) [PubMed: 2606104] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-514. Strain: Wistar. Tissue: Liver.
[4]	Fritz R.S., Kletzien R.F. Submitted (NOV-1989) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 41-515.
[5]	Lubec G., Afjehi-Sadat L. Submitted (NOV-2006) to UniProtKB Cited for: PROTEIN SEQUENCE OF 58-72; 176-192 AND 228-257, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Spinal cord.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07467 mRNA. Translation: CAA30355.1. BC081820 mRNA. Translation: AAH81820.1. M26655, M26653, M26654 Genomic DNA. Translation: AAA41179.1.
IPI	IPI00231637.
PIR	S01233.
RefSeq	NP_058702.1.
UniGene	Rn.11040
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
SMR	P05370. Positions 28-515.
ModBase	Search...
Proteomic databases
PRIDE	P05370.
Genome annotation databases
Ensembl	ENSRNOG00000037254. Rattus norvegicus. [Contig view]
GeneID	24377.
KEGG	rno:24377.
Organism-specific databases
RGD	2645. G6pdx.
Phylogenomic databases
HOVERGEN	P05370.
Enzyme and pathway databases
BRENDA	1.1.1.49. 248.
Gene expression databases
ArrayExpress	P05370.
GermOnline	ENSRNOG00000037254. Rattus norvegicus.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603131.

Entry information

Entry name

G6PD_RAT

Accession

Primary (citable) accession number: P05370

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)