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P05364 (AMPC_ENTCL) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase

EC=3.5.2.6
Alternative name(s):
Cephalosporinase
Gene names
Name:ampC
OrganismEnterobacter cloacae
Taxonomic identifier550 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Subcellular location

Periplasm By similarity.

Miscellaneous

The sequence shown is that of strain P99.

Sequence similarities

Belongs to the class-C beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentPeriplasm
   DomainSignal
   Molecular functionHydrolase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processantibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020
Chain21 – 381361Beta-lactamase
PRO_0000016959

Regions

Region335 – 3373Substrate binding

Sites

Active site841Acyl-ester intermediate
Active site1701Proton acceptor

Natural variations

Natural variant31R → I in strain: MHN1.
Natural variant141I → L in strain: MHN1.
Natural variant211T → A in strain: MHN1.
Natural variant361I → V in strain: MHN1 and Q980R.
Natural variant581P → S in strain: MHN1.
Natural variant1081A → P in strain: MHN1 and Q980R.
Natural variant1521L → V in strain: Q980R.
Natural variant2621N → K in strain: MHN1.
Natural variant3191A → V in strain: Q980R.
Natural variant3621T → K in strain: MHN1.

Secondary structure

................................................................... 381
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P05364 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 90F56ABAF07AA304

FASTA38141,302
        10         20         30         40         50         60 
MMRKSLCCAL LLGISCSALA TPVSEKQLAE VVANTITPLM KAQSVPGMAV AVIYQGKPHY 

        70         80         90        100        110        120 
YTFGKADIAA NKPVTPQTLF ELGSISKTFT GVLGGDAIAR GEISLDDAVT RYWPQLTGKQ 

       130        140        150        160        170        180 
WQGIRMLDLA TYTAGGLPLQ VPDEVTDNAS LLRFYQNWQP QWKPGTTRLY ANASIGLFGA 

       190        200        210        220        230        240 
LAVKPSGMPY EQAMTTRVLK PLKLDHTWIN VPKAEEAHYA WGYRDGKAVR VSPGMLDAQA 

       250        260        270        280        290        300 
YGVKTNVQDM ANWVMANMAP ENVADASLKQ GIALAQSRYW RIGSMYQGLG WEMLNWPVEA 

       310        320        330        340        350        360 
NTVVEGSDSK VALAPLPVAE VNPPAPPVKA SWVHKTGSTG GFGSYVAFIP EKQIGIVMLA 

       370        380 
NTSYPNPARV EAAYHILEAL Q 

« Hide

References

[1]"Sequence and comparative analysis of three Enterobacter cloacae ampC beta-lactamase genes and their products."
Galleni M., Lindberg F., Normark S., Cole S., Honore N., Joris B., Frere J.-M.
Biochem. J. 250:753-760(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MHN1, P99 and Q908R.
[2]"Evolution of an enzyme activity: crystallographic structure at 2-A resolution of cephalosporinase from the ampC gene of Enterobacter cloacae P99 and comparison with a class A penicillinase."
Lobkovsky E., Moews P.C., Liu H., Zhao H., Frere J.-M., Knox J.R.
Proc. Natl. Acad. Sci. U.S.A. 90:11257-11261(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
Strain: P99.
[3]"Crystallographic structure of a phosphonate derivative of the Enterobacter cloacae P99 cephalosporinase: mechanistic interpretation of a beta-lactamase transition-state analog."
Lobkovsky E., Billings E.M., Moews P.C., Rahil J., Pratt R.F., Knox J.R.
Biochemistry 33:6762-6772(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
Strain: P99.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X07274 Genomic DNA. Translation: CAA30257.1.
X08082 Genomic DNA. Translation: CAA30879.1.
X08081 Genomic DNA. Translation: CAA30878.1.
PIRPNKBP. S00404.
PNKBQ. S00405.
PNKBM. S00406.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BLSX-ray2.30A/B21-381[»]
1GCEX-ray1.80A21-381[»]
1RGZX-ray1.37A22-381[»]
1XX2X-ray1.88A/B21-381[»]
1Y54X-ray2.10A21-381[»]
2Q9MX-ray2.05A22-380[»]
2Q9NX-ray2.20A22-380[»]
3S4XX-ray1.95A21-381[»]
ProteinModelPortalP05364.
SMRP05364. Positions 22-380.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP05364.
ChEMBLCHEMBL2725.

Protein family/group databases

MEROPSS12.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP05364.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP05364.

Entry information

Entry nameAMPC_ENTCL
AccessionPrimary (citable) accession number: P05364
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: December 11, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references