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Protein

Beta-lactamase

Gene

ampC

Organism
Enterobacter cloacae
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei84Acyl-ester intermediate1
Active sitei170Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BRENDAi3.5.2.6. 155.
SABIO-RKP05364.

Protein family/group databases

MEROPSiS12.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cephalosporinase
Gene namesi
Name:ampC
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2725.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Add BLAST20
ChainiPRO_000001695921 – 381Beta-lactamaseAdd BLAST361

Proteomic databases

PRIDEiP05364.

Interactioni

Protein-protein interaction databases

STRINGi718254.ENC_42520.

Chemistry databases

BindingDBiP05364.

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 42Combined sources18
Beta strandi46 – 54Combined sources9
Beta strandi57 – 67Combined sources11
Turni68 – 71Combined sources4
Beta strandi79 – 81Combined sources3
Helixi83 – 85Combined sources3
Helixi86 – 99Combined sources14
Helixi109 – 112Combined sources4
Helixi119 – 121Combined sources3
Helixi126 – 130Combined sources5
Helixi148 – 157Combined sources10
Beta strandi166 – 168Combined sources3
Helixi172 – 182Combined sources11
Helixi184 – 186Combined sources3
Helixi190 – 197Combined sources8
Turni198 – 203Combined sources6
Beta strandi207 – 210Combined sources4
Helixi213 – 218Combined sources6
Beta strandi222 – 224Combined sources3
Beta strandi227 – 229Combined sources3
Helixi237 – 241Combined sources5
Helixi247 – 258Combined sources12
Helixi260 – 262Combined sources3
Helixi266 – 275Combined sources10
Beta strandi277 – 282Combined sources6
Beta strandi285 – 287Combined sources3
Beta strandi292 – 297Combined sources6
Helixi300 – 307Combined sources8
Helixi309 – 312Combined sources4
Beta strandi319 – 325Combined sources7
Beta strandi329 – 338Combined sources10
Beta strandi343 – 349Combined sources7
Helixi350 – 352Combined sources3
Beta strandi354 – 362Combined sources9
Helixi366 – 379Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BLSX-ray2.30A/B21-381[»]
1GCEX-ray1.80A21-381[»]
1RGZX-ray1.37A22-381[»]
1XX2X-ray1.88A/B21-381[»]
1Y54X-ray2.10A21-381[»]
2Q9MX-ray2.05A22-380[»]
2Q9NX-ray2.20A22-380[»]
3S4XX-ray1.95A21-381[»]
4XUXX-ray1.75A21-381[»]
5HAIX-ray2.74A21-381[»]
ProteinModelPortaliP05364.
SMRiP05364.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05364.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni335 – 337Substrate binding3

Sequence similaritiesi

Belongs to the class-C beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106FGB. Bacteria.
COG1680. LUCA.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05364-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRKSLCCAL LLGISCSALA TPVSEKQLAE VVANTITPLM KAQSVPGMAV
60 70 80 90 100
AVIYQGKPHY YTFGKADIAA NKPVTPQTLF ELGSISKTFT GVLGGDAIAR
110 120 130 140 150
GEISLDDAVT RYWPQLTGKQ WQGIRMLDLA TYTAGGLPLQ VPDEVTDNAS
160 170 180 190 200
LLRFYQNWQP QWKPGTTRLY ANASIGLFGA LAVKPSGMPY EQAMTTRVLK
210 220 230 240 250
PLKLDHTWIN VPKAEEAHYA WGYRDGKAVR VSPGMLDAQA YGVKTNVQDM
260 270 280 290 300
ANWVMANMAP ENVADASLKQ GIALAQSRYW RIGSMYQGLG WEMLNWPVEA
310 320 330 340 350
NTVVEGSDSK VALAPLPVAE VNPPAPPVKA SWVHKTGSTG GFGSYVAFIP
360 370 380
EKQIGIVMLA NTSYPNPARV EAAYHILEAL Q
Length:381
Mass (Da):41,302
Last modified:November 1, 1988 - v1
Checksum:i90F56ABAF07AA304
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti3R → I in strain: MHN1. 1
Natural varianti14I → L in strain: MHN1. 1
Natural varianti21T → A in strain: MHN1. 1
Natural varianti36I → V in strain: MHN1 and Q980R. 1
Natural varianti58P → S in strain: MHN1. 1
Natural varianti108A → P in strain: MHN1 and Q980R. 1
Natural varianti152L → V in strain: Q980R. 1
Natural varianti262N → K in strain: MHN1. 1
Natural varianti319A → V in strain: Q980R. 1
Natural varianti362T → K in strain: MHN1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07274 Genomic DNA. Translation: CAA30257.1.
X08082 Genomic DNA. Translation: CAA30879.1.
X08081 Genomic DNA. Translation: CAA30878.1.
PIRiS00404. PNKBP.
S00405. PNKBQ.
S00406. PNKBM.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07274 Genomic DNA. Translation: CAA30257.1.
X08082 Genomic DNA. Translation: CAA30879.1.
X08081 Genomic DNA. Translation: CAA30878.1.
PIRiS00404. PNKBP.
S00405. PNKBQ.
S00406. PNKBM.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BLSX-ray2.30A/B21-381[»]
1GCEX-ray1.80A21-381[»]
1RGZX-ray1.37A22-381[»]
1XX2X-ray1.88A/B21-381[»]
1Y54X-ray2.10A21-381[»]
2Q9MX-ray2.05A22-380[»]
2Q9NX-ray2.20A22-380[»]
3S4XX-ray1.95A21-381[»]
4XUXX-ray1.75A21-381[»]
5HAIX-ray2.74A21-381[»]
ProteinModelPortaliP05364.
SMRiP05364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi718254.ENC_42520.

Chemistry databases

BindingDBiP05364.
ChEMBLiCHEMBL2725.

Protein family/group databases

MEROPSiS12.006.

Proteomic databases

PRIDEiP05364.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4106FGB. Bacteria.
COG1680. LUCA.

Enzyme and pathway databases

BRENDAi3.5.2.6. 155.
SABIO-RKP05364.

Miscellaneous databases

EvolutionaryTraceiP05364.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPC_ENTCL
AccessioniPrimary (citable) accession number: P05364
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The sequence shown is that of strain P99.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.