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P05348 (RBS_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase small chain, chloroplastic

Short name=RuBisCO small subunit
EC=4.1.1.39
Gene names
Name:RBCS
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length170 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

Subunit structure

8 large chains + 8 small chains.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the RuBisCO small chain family.

Ontologies

Keywords
   Biological processCalvin cycle
Carbon dioxide fixation
Photorespiration
Photosynthesis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Molecular functionLyase
Monooxygenase
Oxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processchloroplast ribulose bisphosphate carboxylase complex biogenesis

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

photorespiration

Inferred from electronic annotation. Source: UniProtKB-KW

reductive pentose-phosphate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

response to blue light

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to cold

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to far red light

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to red light

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentapoplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cell wall

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast envelope

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast stroma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast thylakoid membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cytosolic ribosome

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

thylakoid lumen

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functioncopper ion binding

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

monooxygenase activity

Inferred from electronic annotation. Source: UniProtKB-KW

ribulose-bisphosphate carboxylase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4747Chloroplast Ref.3
Chain48 – 170123Ribulose bisphosphate carboxylase small chain, chloroplastic
PRO_0000031522

Natural variations

Natural variant531A → I.
Natural variant81 – 822LR → IV.
Natural variant851W → E.
Natural variant921S → V.
Natural variant941V → L.
Natural variant1241N → T.
Natural variant1281Q → L.
Natural variant1321E → V.
Natural variant138 – 1392KS → AA.
Natural variant1421D → G.
Natural variant153 – 1542IK → VR.
Natural variant1631A → L.
Natural variant1651K → G.

Experimental info

Sequence conflict811Missing in CAA68419. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P05348 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 4625A5FDED3C2663

FASTA17019,151
        10         20         30         40         50         60 
MAPTVMMASS ATAVAPFQGL KSTASLPVAR RSSRSLGNVS NGGRIRCMQV WPAYGNKKFE 

        70         80         90        100        110        120 
TLSYLPPLST DDLLKQVDYL LRNGWIPCLE FSKVGFVYRE NSTSPCYYDG RYWTMWKLPM 

       130        140        150        160        170 
FGCNDATQVY KELQEAIKSY PDAFHRVIGF DNIKQTQCVS FIAYKPPGSD 

« Hide

References

[1]"Nucleotide sequence of cDNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase from maize."
Matsuoka M., Kano-Murakami Y., Tanaka Y., Ozeki Y., Ymamoto N.
J. Biochem. 102:673-676(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Nucleotide sequence of a gene encoding corn ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcs)."
Lebrun M., Waksman G., Freyssinet G.
Nucleic Acids Res. 15:4360-4360(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. F2.
[3]"Ribulose-1,5-bisphosphate carboxylase/oxygenase from Zea mays: amino-acid sequence of the small subunit."
Ren L., Salnikow J., Vater J.
Biol. Chem. Hoppe-Seyler 369:609-615(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 48-170.
[4]"Maize rbcS promoter activity depends on sequence elements not found in dicot rbcS promoters."
Schaffner A.R., Sheen J.
Plant Cell 3:997-1012(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00170 mRNA. Translation: BAA00120.1.
X06535 mRNA. Translation: CAA29784.1.
Y00322 Genomic DNA. Translation: CAA68419.1.
S42508 Genomic DNA. Translation: AAD13825.1.
S42568 Genomic DNA. Translation: AAD13826.1.
PIRRKZMS. S00534.
RefSeqNP_001105294.1. NM_001111824.1.
UniGeneZm.72721.

3D structure databases

ProteinModelPortalP05348.
SMRP05348. Positions 49-168.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP05348.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID542212.
KEGGzma:542212.

Organism-specific databases

GrameneP05348.
MaizeGDB62391.

Phylogenomic databases

HOGENOMHOG000141332.
KOK01602.
OMAHAFIRII.

Family and domain databases

Gene3D3.30.190.10. 1 hit.
InterProIPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view]
PfamPF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view]
PRINTSPR00152. RUBISCOSMALL.
SUPFAMSSF55239. SSF55239. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRBS_MAIZE
AccessionPrimary (citable) accession number: P05348
Secondary accession number(s): Q43355
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families