P05348 (RBS_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain, chloroplastic
Short name=RuBisCO small subunit
EC=4.1.1.39

Gene names

Name:

RBCS

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site By similarity.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photorespiration Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	photorespiration Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW response to blue light Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cold Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to far red light Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to red light Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast stroma Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast thylakoid membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene cytosolic ribosome Inferred from electronic annotation. Source: EnsemblPlants/Gramene thylakoid lumen Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	copper ion binding Inferred from electronic annotation. Source: EnsemblPlants/Gramene monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 47	47	Chloroplast Ref.3
	Chain	48 – 170	123	Ribulose bisphosphate carboxylase small chain, chloroplastic		PRO_0000031522
Natural variations
	Natural variant	53	1	A → I.
	Natural variant	81 – 82	2	LR → IV.
	Natural variant	85	1	W → E.
	Natural variant	92	1	S → V.
	Natural variant	94	1	V → L.
	Natural variant	124	1	N → T.
	Natural variant	128	1	Q → L.
	Natural variant	132	1	E → V.
	Natural variant	138 – 139	2	KS → AA.
	Natural variant	142	1	D → G.
	Natural variant	153 – 154	2	IK → VR.
	Natural variant	163	1	A → L.
	Natural variant	165	1	K → G.
Experimental info
	Sequence conflict	81	1	Missing in CAA68419. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05348 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 4625A5FDED3C2663	FASTA	170	19,151
	10 20 30 40 50 60 MAPTVMMASS ATAVAPFQGL KSTASLPVAR RSSRSLGNVS NGGRIRCMQV WPAYGNKKFE 70 80 90 100 110 120 TLSYLPPLST DDLLKQVDYL LRNGWIPCLE FSKVGFVYRE NSTSPCYYDG RYWTMWKLPM 130 140 150 160 170 FGCNDATQVY KELQEAIKSY PDAFHRVIGF DNIKQTQCVS FIAYKPPGSD

References

[1]	"Nucleotide sequence of cDNA encoding the small subunit of ribulose-1,5-bisphosphate carboxylase from maize." Matsuoka M., Kano-Murakami Y., Tanaka Y., Ozeki Y., Ymamoto N. J. Biochem. 102:673-676(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Nucleotide sequence of a gene encoding corn ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcs)." Lebrun M., Waksman G., Freyssinet G. Nucleic Acids Res. 15:4360-4360(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. F2.
[3]	"Ribulose-1,5-bisphosphate carboxylase/oxygenase from Zea mays: amino-acid sequence of the small subunit." Ren L., Salnikow J., Vater J. Biol. Chem. Hoppe-Seyler 369:609-615(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 48-170.
[4]	"Maize rbcS promoter activity depends on sequence elements not found in dicot rbcS promoters." Schaffner A.R., Sheen J. Plant Cell 3:997-1012(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00170 mRNA. Translation: BAA00120.1. X06535 mRNA. Translation: CAA29784.1. Y00322 Genomic DNA. Translation: CAA68419.1. S42508 Genomic DNA. Translation: AAD13825.1. S42568 Genomic DNA. Translation: AAD13826.1.
PIR	RKZMS. S00534.
RefSeq	NP_001105294.1. NM_001111824.1.
UniGene	Zm.72721.
3D structure databases
ProteinModelPortal	P05348.
SMR	P05348. Positions 49-168.
ModBase	Search...
Proteomic databases
PRIDE	P05348.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542212.
KEGG	zma:542212.
Organism-specific databases
Gramene	P05348.
MaizeGDB	62391.
Phylogenomic databases
HOGENOM	HOG000141332.
KO	K01602.
OMA	AFPATRK.
Family and domain databases
Gene3D	3.30.190.10. 1 hit.
InterPro	IPR024681. RuBisCO_sc. IPR000894. RuBisCO_sc_dom. IPR024680. RuBisCO_ssu_N. [Graphical view]
Pfam	PF12338. RbcS. 1 hit. PF00101. RuBisCO_small. 1 hit. [Graphical view]
PRINTS	PR00152. RUBISCOSMALL.
SUPFAM	SSF55239. RuBisCO_small. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RBS_MAIZE

Accession

Primary (citable) accession number: P05348
Secondary accession number(s): Q43355

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program