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Reviewed, UniProtKB/Swiss-Prot P05336 (ADH1_HORVU)

Last modified June 16, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alcohol dehydrogenase 1
    EC=1.1.1.1
Gene names
Name: ADH1
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

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Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactor

Binds 2 zinc ions per subunit.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
NAD
Zinc
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalcohol dehydrogenase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Alcohol dehydrogenase 1
PRO_0000160699

Regions

Nucleotide binding202 – 2076NAD By similarity
Nucleotide binding295 – 2973NAD By similarity

Sites

Metal binding471Zinc 1; catalytic By similarity
Metal binding691Zinc 1; catalytic By similarity
Metal binding991Zinc 2 By similarity
Metal binding1021Zinc 2 By similarity
Metal binding1051Zinc 2 By similarity
Metal binding1131Zinc 2 By similarity
Metal binding1771Zinc 1; catalytic By similarity
Binding site2261NAD By similarity
Binding site2311NAD By similarity
Binding site3721NAD By similarity

Experimental info

Sequence conflict741I → T in CAB51640. Ref.2
Sequence conflict931P → A in CAB51640. Ref.2
Sequence conflict126 – 1272IG → NR in CAB51640. Ref.2
Sequence conflict1341S → F in CAB51640. Ref.2
Sequence conflict2051A → S in CAB51640. Ref.2
Sequence conflict2101A → T in CAB51640. Ref.2
Sequence conflict2251V → I in CAB51640. Ref.2
Sequence conflict2301V → F in CAB51640. Ref.2
Sequence conflict2481D → A in CAB51640. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
P05336-1 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 3A94ABDD2967FCB7

FASTA37940,884
        10         20         30         40         50         60 
MATAGKVIKC KAAVAWEAGK PLTMEEVEVA PPQAMEVRVK ILFTSLCHTD VYFWEAKGQI 

        70         80         90        100        110        120 
PMFPRIFGHE AGGIVESVGE GVTDVAPGDH VLPVFTGECK ECPHCKSAES NMCDLLRINT 

       130        140        150        160        170        180 
DRGVMIGDGK SRFSIGGKPI YHFVGTSTFS EYTVMHVGCV AKINPEAPLD KVCVLSCGIS 

       190        200        210        220        230        240 
TGLGASINVA KPPKGSTVAI FGLGAVGLAA AEGARIAGAS RIIGVDLNAV RFEEARKFGC 

       250        260        270        280        290        300 
TEFVNPKDHT KPVQQVLADM TNGGVDRSVE CTGNVNAMIQ AFECVHDGWG VAVLVGVPHK 

       310        320        330        340        350        360 
DAEFKTHPMN FLNERTLKGT FFGNFKPRTD LPNVVEMYMK KELEVEKFIT HSVPFSEINT 

       370 
AFDLMAKGEG IRCIIRMDN

« Hide

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References

[1]"Nucleotide sequence of a complete barley alcohol dehydrogenase 1 cDNA."
Good A., Pelcher L.E., Crosby W.L.
Nucleic Acids Res. 16:7182-7182(1988) [PubMed: 3405765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Himalaya.
[2]"Molecular analysis of the alcohol dehydrogenase gene family of barley."
Trick M., Dennis E.S., Edwards K.J.R., Peacock W.J.
Plant Mol. Biol. 11:147-160(1988) [Agricola: IND92000066]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 74-304.
Strain: cv. Proctor.

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Cross-references

Sequence databases

X07774 mRNA. Translation: CAA30600.1.
X12732 Genomic DNA. Translation: CAB51640.1.
PIRS01893.
UniGeneHv.22954

3D structure databases

HSSPHSSP built from PDB template 1M6H based on UniProtKB P11766.
ModBaseSearch...

Organism-specific databases

GrameneP05336.

Enzyme and pathway databases

BRENDA1.1.1.1. 283.

Family and domain databases

InterProIPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn.
IPR013149. ADH_Zn-bd.
IPR002328. ADH_Zn_CS.
[Graphical view]
PANTHERPTHR11695. ADH_Sf_Zn. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
PROSITEPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameADH1_HORVU
AccessionPrimary (citable) accession number: P05336
Secondary accession number(s): Q40011 expand/collapse secondary AC list , Q40012, Q40013, Q40014
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: June 16, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents