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P05328 (TRPG_ASPNG) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Multifunctional tryptophan biosynthesis protein

Including the following 3 domains:

  1. Anthranilate synthase component 2
    Short name=AS
    EC=4.1.3.27
    Alternative name(s):
    Anthranilate synthase, glutamine amidotransferase component
  2. Indole-3-glycerol phosphate synthase
    Short name=IGPS
    EC=4.1.1.48
  3. N-(5'-phosphoribosyl)anthranilate isomerase
    Short name=PRAI
    EC=5.3.1.24
Gene names
Name:trpC
OrganismAspergillus niger
Taxonomic identifier5061 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length770 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities. HAMAP-Rule MF_00135

Catalytic activity

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP-Rule MF_00135

1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O. HAMAP-Rule MF_00135

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate. HAMAP-Rule MF_00135

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5. HAMAP-Rule MF_00135

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 770770Multifunctional tryptophan biosynthesis protein HAMAP-Rule MF_00135
PRO_0000056855

Regions

Domain25 – 225201Glutamine amidotransferase type-1
Region255 – 519265Indole-3-glycerol phosphate synthase HAMAP-Rule MF_00135
Region535 – 770236N-(5'-phosphoribosyl)anthranilate isomerase HAMAP-Rule MF_00135

Sites

Active site1041For GATase activity By similarity
Active site1991For GATase activity By similarity
Active site2011For GATase activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P05328 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 36D8DE5B23097012

FASTA77082,910
        10         20         30         40         50         60 
MADSGLVDHS PHHPTKAAQL STASNVILID NYDSFTWNVY QYLVLEGATV NVFRNDQITL 

        70         80         90        100        110        120 
EELIAKKPTQ LVISPGPGHP ETDAGISSAA IQYFSGKIPI FGVCMGQQCI ITCFGGKVDV 

       130        140        150        160        170        180 
TGEILHGKTS PLKHDGKGAY EGLPGSLAVT RYHSLAGTHA TIPDCLEVSS SVQLADDSNK 

       190        200        210        220        230        240 
DVIMGVRHKK LAVEGVQFHP ESILTEYGRI MFRNFLKLTA GTWEGNGKHF GEQSSTTKAT 

       250        260        270        280        290        300 
VPSNPPPKTD KKLSILERIY DHRRAAVAVQ KTIPSQRPAD LQAAYDLNLA PPQIPFPARL 

       310        320        330        340        350        360 
RQSPYPLSLM AEIKRASPSK GMIAENACAP AQARQYAKAG ASVISVLTEP EWFKGSIDDL 

       370        380        390        400        410        420 
RAVRQSLEGM TNRPAILRKE FVFDEYQILE ARLAGADTVL LIVKMLSVEL LTRLYHYSRS 

       430        440        450        460        470        480 
LGMEPLVEVN TPEEMKIAVD LGAEVIGVNN RDLTSFEVDL GTTSRLMDQV PSSTIVCALS 

       490        500        510        520        530        540 
GISGPKDVEA YKKEGVKAIL VGEALMRAAD TATFIAELLG GSSQTVSSES RRSPLVKICG 

       550        560        570        580        590        600 
TRSEEAARAA IEAGADLIGI IMVQGRTGCV PDDVALPISQ VVRSTPKPAS QALHTSQEPP 

       610        620        630        640        650        660 
AATSVEYFDH SAKILRHPSR ALLVGVFQNQ PLDYILSQQQ KLGLDVVQLH GSEPLEWAKL 

       670        680        690        700        710        720 
IPVPVIRKFG LDEPAIARRA YHSLPLLDSG VGGTGELLDQ SRVQNVLDKD CGLRVILAGG 

       730        740        750        760        770 
LDPTNVAGIV QKLGESGRKV VGVDVSSGVE SDGAQDLNKI RAFVQAVRGL 

« Hide

References

[1]"Nucleotide sequence of the Aspergillus niger trpC gene: structural relationship with analogous genes of other organisms."
Kos T., Kuijvenhoven A., Hessing H.G.M., Pouwels P.H., van den Hondel C.A.M.J.J.
Curr. Genet. 13:137-144(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 401.
[2]"Isolation and characterization of the Aspergillus niger trpC gene."
Kos A., Kuijvenhoven J., Wernars K., Bos C.J., van den Broek H.W.J., Pouwels P.H., van den Hondel C.A.M.J.J.
Gene 39:231-238(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-69 AND 392-433.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X07071 Genomic DNA. Translation: CAA30107.1.
M14403 Genomic DNA. Translation: AAA32710.1.
M14404 Genomic DNA. Translation: AAA32709.1.
PIRS00643.

3D structure databases

ProteinModelPortalP05328.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0134.

Enzyme and pathway databases

UniPathwayUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Family and domain databases

Gene3D3.20.20.70. 3 hits.
HAMAPMF_00135. PRAI.
InterProIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PANTHERPTHR11922:SF3. PTHR11922:SF3. 1 hit.
PfamPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMSSF51366. SSF51366. 3 hits.
TIGRFAMsTIGR00566. trpG_papA. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRPG_ASPNG
AccessionPrimary (citable) accession number: P05328
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: February 19, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways