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Protein

Multifunctional tryptophan biosynthesis protein

Gene

trpC

Organism
Aspergillus niger
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (trpC), Multifunctional tryptophan biosynthesis protein (ABL_04480), Multifunctional tryptophan biosynthesis protein (ATCC64974_101700)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (trpC), Multifunctional tryptophan biosynthesis protein (ABL_04480), Multifunctional tryptophan biosynthesis protein (ATCC64974_101700)
  4. Multifunctional tryptophan biosynthesis protein (trpC), Multifunctional tryptophan biosynthesis protein (ABL_04480), Multifunctional tryptophan biosynthesis protein (ATCC64974_101700)
  5. Tryptophan synthase (ABL_09212), Tryptophan synthase (ATCC64974_71060), Tryptophan synthase (ATCC64974_60670), Tryptophan synthase (ATCC64974_41610), Tryptophan synthase (ABL_01002), Tryptophan synthase (CAN33_14670), Tryptophan synthase (CAN33_27565)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Nucleophile; for GATase activityBy similarity1
Binding sitei108GlutamineBy similarity1
Active sitei199For GATase activityBy similarity1
Active sitei201For GATase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Isomerase, Lyase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040
UPA00035; UER00042
UPA00035; UER00043

Protein family/group databases

MEROPSiC26.959

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:trpC
OrganismiAspergillus niger
Taxonomic identifieri5061 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000568551 – 770Multifunctional tryptophan biosynthesis proteinAdd BLAST770

Structurei

3D structure databases

ProteinModelPortaliP05328
SMRiP05328
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 225Glutamine amidotransferase type-1Add BLAST201

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 78Glutamine bindingBy similarity3
Regioni154 – 155Glutamine bindingBy similarity2
Regioni255 – 519Indole-3-glycerol phosphate synthaseAdd BLAST265
Regioni535 – 770N-(5'-phosphoribosyl)anthranilate isomeraseAdd BLAST236

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0026 Eukaryota
KOG4201 Eukaryota
KOG4202 Eukaryota
COG0134 LUCA
COG0135 LUCA
COG0512 LUCA

Family and domain databases

CDDicd00331 IGPS, 1 hit
cd00405 PRAI, 1 hit
Gene3Di3.20.20.70, 3 hits
3.40.50.880, 1 hit
HAMAPiMF_00135 PRAI, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR016302 Anthranilate_synth_II
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR013798 Indole-3-glycerol_P_synth
IPR001468 Indole-3-GlycerolPSynthase_CS
IPR001240 PRAI
IPR011060 RibuloseP-bd_barrel
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00218 IGPS, 1 hit
PF00697 PRAI, 1 hit
PIRSFiPIRSF001382 TrpG-trpC-trpF, 1 hit
SUPFAMiSSF51366 SSF51366, 3 hits
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS00614 IGPS, 1 hit

Sequencei

Sequence statusi: Complete.

P05328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSGLVDHS PHHPTKAAQL STASNVILID NYDSFTWNVY QYLVLEGATV
60 70 80 90 100
NVFRNDQITL EELIAKKPTQ LVISPGPGHP ETDAGISSAA IQYFSGKIPI
110 120 130 140 150
FGVCMGQQCI ITCFGGKVDV TGEILHGKTS PLKHDGKGAY EGLPGSLAVT
160 170 180 190 200
RYHSLAGTHA TIPDCLEVSS SVQLADDSNK DVIMGVRHKK LAVEGVQFHP
210 220 230 240 250
ESILTEYGRI MFRNFLKLTA GTWEGNGKHF GEQSSTTKAT VPSNPPPKTD
260 270 280 290 300
KKLSILERIY DHRRAAVAVQ KTIPSQRPAD LQAAYDLNLA PPQIPFPARL
310 320 330 340 350
RQSPYPLSLM AEIKRASPSK GMIAENACAP AQARQYAKAG ASVISVLTEP
360 370 380 390 400
EWFKGSIDDL RAVRQSLEGM TNRPAILRKE FVFDEYQILE ARLAGADTVL
410 420 430 440 450
LIVKMLSVEL LTRLYHYSRS LGMEPLVEVN TPEEMKIAVD LGAEVIGVNN
460 470 480 490 500
RDLTSFEVDL GTTSRLMDQV PSSTIVCALS GISGPKDVEA YKKEGVKAIL
510 520 530 540 550
VGEALMRAAD TATFIAELLG GSSQTVSSES RRSPLVKICG TRSEEAARAA
560 570 580 590 600
IEAGADLIGI IMVQGRTGCV PDDVALPISQ VVRSTPKPAS QALHTSQEPP
610 620 630 640 650
AATSVEYFDH SAKILRHPSR ALLVGVFQNQ PLDYILSQQQ KLGLDVVQLH
660 670 680 690 700
GSEPLEWAKL IPVPVIRKFG LDEPAIARRA YHSLPLLDSG VGGTGELLDQ
710 720 730 740 750
SRVQNVLDKD CGLRVILAGG LDPTNVAGIV QKLGESGRKV VGVDVSSGVE
760 770
SDGAQDLNKI RAFVQAVRGL
Length:770
Mass (Da):82,910
Last modified:November 1, 1988 - v1
Checksum:i36D8DE5B23097012
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07071 Genomic DNA Translation: CAA30107.1
M14403 Genomic DNA Translation: AAA32710.1
M14404 Genomic DNA Translation: AAA32709.1
PIRiS00643

Similar proteinsi

Entry informationi

Entry nameiTRPG_ASPNG
AccessioniPrimary (citable) accession number: P05328
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health