60S acidic ribosomal protein P2-alpha - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P05319 (RLA2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 130. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
60S acidic ribosomal protein P2-alpha
Short name=P2A

Alternative name(s):
A2
L12EIB
L44
YP2alpha

Gene names

Name:	RPP2A
Synonyms:	L12EIB, RPA2, RPL44, RPLA2
Ordered Locus Names:	YOL039W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the elongation step of protein synthesis. HAMAP-Rule MF_01478
Subunit structure	Heterodimer of P1B-P2A. Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A. Ref.8 Ref.11 Ref.14
Subcellular location	Cytoplasm Ref.12.
Post-translational modification	Phosphorylation is not involved in the interaction of the acidic P proteins with the ribosome, however it is suggested to affect the ribosome activity and to participate in a possible ribosome regulatory mechanism. HAMAP-Rule MF_01478 The N-terminus is not modified. HAMAP-Rule MF_01478
Miscellaneous	Yeasts contain 4 individual small ribosomal A proteins (RPA) which can be classified into two couples of similar but not identical sequences. Each couple is distinctly related to one of the two A proteins present in multicellular organisms. HAMAP-Rule MF_01478 Present with 428000 molecules/cell in log phase SD medium. HAMAP-Rule MF_01478
Sequence similarities	Belongs to the ribosomal protein L12P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.8. Source: SGD translational elongation Inferred from sequence or structural similarity PubMed 9175862 Ref.8. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.8. Source: SGD
Molecular_function	structural constituent of ribosome Traceable author statement Ref.8. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPP0	P05317	2	EBI-15456,EBI-15447
RPP1B	P10622	2	EBI-15456,EBI-15460

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 106

106

60S acidic ribosomal protein P2-alpha HAMAP-Rule MF_01478

PRO_0000157681

Amino acid modifications

Modified residue

Phosphothreonine Ref.17

Modified residue

Phosphoserine Ref.17

Modified residue

Phosphoserine Ref.17

Modified residue

Phosphoserine Ref.17

Modified residue

Phosphoserine Ref.15 Ref.16 Ref.17

Sequences

Sequence

Length

Mass (Da)

Tools

P05319 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 22275AFA35E1A32E
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FASTA

106

10,746

        10         20         30         40         50         60 
MKYLAAYLLL NAAGNTPDAT KIKAILESVG IEIEDEKVSS VLSALEGKSV DELITEGNEK 

        70         80         90        100 
LAAVPAAGPA SAGGAAAASG DAAAEEEKEE EAAEESDDDM GFGLFD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA and deduced amino acid sequence of acidic ribosomal protein A2 from Saccharomyces cerevisiae." Mitsui K., Tsurugi K. Nucleic Acids Res. 16:3575-3575(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: IFO 40028.
[2]	"Independent genes coding for three acidic proteins of the large ribosomal subunit from Saccharomyces cerevisiae." Remacha M., Saenz-Robles M.T., Vilella M.D., Ballesta J.P.G. J. Biol. Chem. 263:9094-9101(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A family of genes encode the multiple forms of the Saccharomyces cerevisiae ribosomal proteins equivalent to the Escherichia coli L12 protein and a single form of the L10-equivalent ribosomal protein." Newton C.H., Shimmin L.C., Yee J., Dennis P.P. J. Bacteriol. 172:579-588(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SR26-12C.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"The acidic phosphoproteins from Saccharomyces cerevisiae ribosomes. NH2-terminal acetylation is a conserved difference between P1 and P2 proteins." Santos C., Ortiz-Reyes B., Naranda T., Remacha M., Ballesta J.P.G. Biochemistry 32:4231-4236(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-6.
[8]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[9]	"Phosphorylation of the yeast ribosomal stalk. Functional effects and enzymes involved in the process." Ballesta J.P.G., Rodriguez-Gabriel M.A., Bou G., Briones E., Zambrano R., Remacha M. FEMS Microbiol. Rev. 23:537-550(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE OF PHOSPHORYLATION.
[10]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ANALYSIS OF N-TERMINUS.
[11]	"Asymmetric interactions between the acidic P1 and P2 proteins in the Saccharomyces cerevisiae ribosomal stalk." Guarinos E., Remacha M., Ballesta J.P.G. J. Biol. Chem. 276:32474-32479(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RPP1B.
[12]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[13]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[14]	"Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins." Krokowski D., Boguszewska A., Abramczyk D., Liljas A., Tchorzewski M., Grankowski N. Mol. Microbiol. 60:386-400(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RPP0 AND RPP1B.
[15]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, MASS SPECTROMETRY. Strain: ADR376.
[16]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, MASS SPECTROMETRY.
[17]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-16; SER-39; SER-49; SER-79 AND SER-96, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X06958 mRNA. Translation: CAA30028.1.
J03760 Genomic DNA. Translation: AAA34971.1.
M26503 Genomic DNA. Translation: AAA34735.1.
Z74781 Genomic DNA. Translation: CAA99041.1.
AY692793 Genomic DNA. Translation: AAT92812.1.
BK006948 Genomic DNA. Translation: DAA10743.1.

PIR

R5BYIB. B28104.

RefSeq

NP_014603.1. NM_001183293.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IZS	electron microscopy	-	v/w	1-106	[»]

ProteinModelPortal

P05319.

SMR

P05319. Positions 1-68.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2506N.

IntAct

P05319. 20 interactions.

MINT

MINT-531798.

STRING

4932.YOL039W.

Protein family/group databases

Allergome

9537. Sac c P2.

Proteomic databases

PaxDb

P05319.

PeptideAtlas

P05319.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOL039W; YOL039W; YOL039W.

GeneID

854118.

KEGG

sce:YOL039W.

Organism-specific databases

CYGD

YOL039w.

SGD

S000005399. RPP2A.

Phylogenomic databases

eggNOG

COG2058.

GeneTree

ENSGT00550000074828.

HOGENOM

HOG000229897.

K02943.

OMA

NIEDVMA.

OrthoDB

EOG4CZFRQ.

Gene expression databases

Genevestigator

P05319.

GermOnline

YOL039W. Saccharomyces cerevisiae.

Family and domain databases

HAMAP

MF_01478. Ribosomal_L12_arch.

InterPro

IPR001813. Ribosomal_60S.
[Graphical view]

Pfam

PF00428. Ribosomal_60s. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

975819.

Entry information

Entry name

RLA2_YEAST

Accession

Primary (citable) accession number: P05319
Secondary accession number(s): D6W227

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	April 3, 2013
This is version 130 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents