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P05319

- RLA2_YEAST

UniProt

P05319 - RLA2_YEAST

Protein

60S acidic ribosomal protein P2-alpha

Gene

RPP2A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Plays an important role in the elongation step of protein synthesis.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein kinase activator activity Source: SGD
    3. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. positive regulation of protein kinase activity Source: GOC
    3. translational elongation Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33453-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S acidic ribosomal protein P2-alpha
    Short name:
    P2A
    Alternative name(s):
    A2
    L12EIB
    L44
    YP2alpha
    Gene namesi
    Name:RPP2A
    Synonyms:L12EIB, RPA2, RPL44, RPLA2
    Ordered Locus Names:YOL039W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL039w.
    SGDiS000005399. RPP2A.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Protein family/group databases

    Allergomei9537. Sac c P2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10610660S acidic ribosomal protein P2-alphaPRO_0000157681Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei16 – 161Phosphothreonine1 Publication
    Modified residuei40 – 401Phosphoserine1 Publication
    Modified residuei43 – 431Phosphoserine1 Publication
    Modified residuei49 – 491Phosphoserine2 Publications
    Modified residuei96 – 961Phosphoserine3 Publications

    Post-translational modificationi

    Phosphorylation is not involved in the interaction of the acidic P proteins with the ribosome, however it is suggested to affect the ribosome activity and to participate in a possible ribosome regulatory mechanism.3 Publications
    The N-terminus is not modified.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP05319.
    PaxDbiP05319.
    PeptideAtlasiP05319.

    Expressioni

    Gene expression databases

    GenevestigatoriP05319.

    Interactioni

    Subunit structurei

    Heterodimer of P1B-P2A. Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RPP0P053172EBI-15456,EBI-15447
    RPP1BP106222EBI-15456,EBI-15460

    Protein-protein interaction databases

    BioGridi34363. 64 interactions.
    DIPiDIP-2506N.
    IntActiP05319. 22 interactions.
    MINTiMINT-531798.
    STRINGi4932.YOL039W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IZSelectron microscopy-v/w1-106[»]
    ProteinModelPortaliP05319.
    SMRiP05319. Positions 1-68.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L12P family.Curated

    Phylogenomic databases

    eggNOGiCOG2058.
    GeneTreeiENSGT00550000074828.
    HOGENOMiHOG000229897.
    KOiK02943.
    OMAiAEIDETK.
    OrthoDBiEOG708WC9.

    Family and domain databases

    HAMAPiMF_01478. Ribosomal_L12_arch.
    InterProiIPR001813. Ribosomal_L10/L12.
    IPR027534. Ribosomal_L12.
    [Graphical view]
    PfamiPF00428. Ribosomal_60s. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05319-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKYLAAYLLL NAAGNTPDAT KIKAILESVG IEIEDEKVSS VLSALEGKSV    50
    DELITEGNEK LAAVPAAGPA SAGGAAAASG DAAAEEEKEE EAAEESDDDM 100
    GFGLFD 106
    Length:106
    Mass (Da):10,746
    Last modified:November 1, 1988 - v1
    Checksum:i22275AFA35E1A32E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06958 mRNA. Translation: CAA30028.1.
    J03760 Genomic DNA. Translation: AAA34971.1.
    M26503 Genomic DNA. Translation: AAA34735.1.
    Z74781 Genomic DNA. Translation: CAA99041.1.
    AY692793 Genomic DNA. Translation: AAT92812.1.
    BK006948 Genomic DNA. Translation: DAA10743.1.
    PIRiB28104. R5BYIB.
    RefSeqiNP_014603.1. NM_001183293.1.

    Genome annotation databases

    EnsemblFungiiYOL039W; YOL039W; YOL039W.
    GeneIDi854118.
    KEGGisce:YOL039W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X06958 mRNA. Translation: CAA30028.1 .
    J03760 Genomic DNA. Translation: AAA34971.1 .
    M26503 Genomic DNA. Translation: AAA34735.1 .
    Z74781 Genomic DNA. Translation: CAA99041.1 .
    AY692793 Genomic DNA. Translation: AAT92812.1 .
    BK006948 Genomic DNA. Translation: DAA10743.1 .
    PIRi B28104. R5BYIB.
    RefSeqi NP_014603.1. NM_001183293.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IZS electron microscopy - v/w 1-106 [» ]
    ProteinModelPortali P05319.
    SMRi P05319. Positions 1-68.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34363. 64 interactions.
    DIPi DIP-2506N.
    IntActi P05319. 22 interactions.
    MINTi MINT-531798.
    STRINGi 4932.YOL039W.

    Protein family/group databases

    Allergomei 9537. Sac c P2.

    Proteomic databases

    MaxQBi P05319.
    PaxDbi P05319.
    PeptideAtlasi P05319.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL039W ; YOL039W ; YOL039W .
    GeneIDi 854118.
    KEGGi sce:YOL039W.

    Organism-specific databases

    CYGDi YOL039w.
    SGDi S000005399. RPP2A.

    Phylogenomic databases

    eggNOGi COG2058.
    GeneTreei ENSGT00550000074828.
    HOGENOMi HOG000229897.
    KOi K02943.
    OMAi AEIDETK.
    OrthoDBi EOG708WC9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33453-MONOMER.

    Miscellaneous databases

    NextBioi 975819.

    Gene expression databases

    Genevestigatori P05319.

    Family and domain databases

    HAMAPi MF_01478. Ribosomal_L12_arch.
    InterProi IPR001813. Ribosomal_L10/L12.
    IPR027534. Ribosomal_L12.
    [Graphical view ]
    Pfami PF00428. Ribosomal_60s. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA and deduced amino acid sequence of acidic ribosomal protein A2 from Saccharomyces cerevisiae."
      Mitsui K., Tsurugi K.
      Nucleic Acids Res. 16:3575-3575(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: IFO 40028.
    2. "Independent genes coding for three acidic proteins of the large ribosomal subunit from Saccharomyces cerevisiae."
      Remacha M., Saenz-Robles M.T., Vilella M.D., Ballesta J.P.G.
      J. Biol. Chem. 263:9094-9101(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "A family of genes encode the multiple forms of the Saccharomyces cerevisiae ribosomal proteins equivalent to the Escherichia coli L12 protein and a single form of the L10-equivalent ribosomal protein."
      Newton C.H., Shimmin L.C., Yee J., Dennis P.P.
      J. Bacteriol. 172:579-588(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SR26-12C.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. "The acidic phosphoproteins from Saccharomyces cerevisiae ribosomes. NH2-terminal acetylation is a conserved difference between P1 and P2 proteins."
      Santos C., Ortiz-Reyes B., Naranda T., Remacha M., Ballesta J.P.G.
      Biochemistry 32:4231-4236(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-6.
    8. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    9. "Phosphorylation of the yeast ribosomal stalk. Functional effects and enzymes involved in the process."
      Ballesta J.P.G., Rodriguez-Gabriel M.A., Bou G., Briones E., Zambrano R., Remacha M.
      FEMS Microbiol. Rev. 23:537-550(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE OF PHOSPHORYLATION.
    10. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ANALYSIS OF N-TERMINUS.
    11. "Asymmetric interactions between the acidic P1 and P2 proteins in the Saccharomyces cerevisiae ribosomal stalk."
      Guarinos E., Remacha M., Ballesta J.P.G.
      J. Biol. Chem. 276:32474-32479(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RPP1B.
    12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    14. "Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins."
      Krokowski D., Boguszewska A., Abramczyk D., Liljas A., Tchorzewski M., Grankowski N.
      Mol. Microbiol. 60:386-400(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RPP0 AND RPP1B.
    15. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    16. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-16; SER-49 AND SER-96, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    17. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40; SER-43; SER-49 AND SER-96, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRLA2_YEAST
    AccessioniPrimary (citable) accession number: P05319
    Secondary accession number(s): D6W227
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Yeasts contain 4 individual small ribosomal A proteins (RPA) which can be classified into two couples of similar but not identical sequences. Each couple is distinctly related to one of the two A proteins present in multicellular organisms.
    Present with 428000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3