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Protein

60S acidic ribosomal protein P2-alpha

Gene

RPP2A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the elongation step of protein synthesis.

GO - Molecular functioni

  • large ribosomal subunit rRNA binding Source: GO_Central
  • protein kinase activator activity Source: SGD
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • translational elongation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-33453-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S acidic ribosomal protein P2-alpha
Short name:
P2A
Alternative name(s):
A2
L12EIB
L44
YP2alpha
Gene namesi
Name:RPP2A
Synonyms:L12EIB, RPA2, RPL44, RPLA2
Ordered Locus Names:YOL039W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL039W.
SGDiS000005399. RPP2A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD
  • preribosome, large subunit precursor Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Protein family/group databases

Allergomei9537. Sac c P2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10610660S acidic ribosomal protein P2-alphaPRO_0000157681Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki2 – 2Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei16 – 161PhosphothreonineCombined sources
Modified residuei40 – 401PhosphoserineCombined sources
Modified residuei43 – 431PhosphoserineCombined sources
Cross-linki48 – 48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei49 – 491PhosphoserineCombined sources
Modified residuei96 – 961PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation is not involved in the interaction of the acidic P proteins with the ribosome, however it is suggested to affect the ribosome activity and to participate in a possible ribosome regulatory mechanism.
The N-terminus is not modified.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP05319.
TopDownProteomicsiP05319.

PTM databases

iPTMnetiP05319.

Interactioni

Subunit structurei

Heterodimer of P1B-P2A. Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPP0P053172EBI-15456,EBI-15447
RPP1BP106222EBI-15456,EBI-15460

Protein-protein interaction databases

BioGridi34363. 65 interactions.
DIPiDIP-2506N.
IntActiP05319. 22 interactions.
MINTiMINT-531798.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Ielectron microscopy8.80Bv/Bw1-106[»]
ProteinModelPortaliP05319.
SMRiP05319. Positions 1-68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L12P family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074828.
HOGENOMiHOG000229897.
InParanoidiP05319.
KOiK02943.
OMAiKGKNVND.
OrthoDBiEOG092C5X8C.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiIPR027534. Ribosomal_L12.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYLAAYLLL NAAGNTPDAT KIKAILESVG IEIEDEKVSS VLSALEGKSV
60 70 80 90 100
DELITEGNEK LAAVPAAGPA SAGGAAAASG DAAAEEEKEE EAAEESDDDM

GFGLFD
Length:106
Mass (Da):10,746
Last modified:November 1, 1988 - v1
Checksum:i22275AFA35E1A32E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06958 mRNA. Translation: CAA30028.1.
J03760 Genomic DNA. Translation: AAA34971.1.
M26503 Genomic DNA. Translation: AAA34735.1.
Z74781 Genomic DNA. Translation: CAA99041.1.
AY692793 Genomic DNA. Translation: AAT92812.1.
BK006948 Genomic DNA. Translation: DAA10743.1.
PIRiB28104. R5BYIB.
RefSeqiNP_014603.1. NM_001183293.1.

Genome annotation databases

EnsemblFungiiYOL039W; YOL039W; YOL039W.
GeneIDi854118.
KEGGisce:YOL039W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06958 mRNA. Translation: CAA30028.1.
J03760 Genomic DNA. Translation: AAA34971.1.
M26503 Genomic DNA. Translation: AAA34735.1.
Z74781 Genomic DNA. Translation: CAA99041.1.
AY692793 Genomic DNA. Translation: AAT92812.1.
BK006948 Genomic DNA. Translation: DAA10743.1.
PIRiB28104. R5BYIB.
RefSeqiNP_014603.1. NM_001183293.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V6Ielectron microscopy8.80Bv/Bw1-106[»]
ProteinModelPortaliP05319.
SMRiP05319. Positions 1-68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34363. 65 interactions.
DIPiDIP-2506N.
IntActiP05319. 22 interactions.
MINTiMINT-531798.

Protein family/group databases

Allergomei9537. Sac c P2.

PTM databases

iPTMnetiP05319.

Proteomic databases

MaxQBiP05319.
TopDownProteomicsiP05319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL039W; YOL039W; YOL039W.
GeneIDi854118.
KEGGisce:YOL039W.

Organism-specific databases

EuPathDBiFungiDB:YOL039W.
SGDiS000005399. RPP2A.

Phylogenomic databases

GeneTreeiENSGT00550000074828.
HOGENOMiHOG000229897.
InParanoidiP05319.
KOiK02943.
OMAiKGKNVND.
OrthoDBiEOG092C5X8C.

Enzyme and pathway databases

BioCyciYEAST:G3O-33453-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiP05319.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiIPR027534. Ribosomal_L12.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLA2_YEAST
AccessioniPrimary (citable) accession number: P05319
Secondary accession number(s): D6W227
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: September 7, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Yeasts contain 4 individual small ribosomal A proteins (RPA) which can be classified into two couples of similar but not identical sequences. Each couple is distinctly related to one of the two A proteins present in multicellular organisms.
Present with 428000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.