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Protein

60S acidic ribosomal protein P2-alpha

Gene

RPP2A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

The 4 small acidic ribosomal P-proteins from yeast can be classified into two couples of similar but not identical sequences. Each couple (P1A/P1B and P2A/P2B) is distinctly related to one of the two acidic ribosomal P-proteins P1/P2 present in multicellular organisms.1 Publication
Present with 428000 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • protein kinase activator activity Source: SGD
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • translational elongation Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-33453-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S acidic ribosomal protein P2-alpha1 Publication
Short name:
A2
Alternative name(s):
L12eIB
L44
Large ribosomal subunit protein P2-A1 Publication
Short name:
P2A
YP2alpha
Gene namesi
Name:RPP2A1 Publication
Synonyms:L12EIB, RPA2, RPL44, RPLA2
Ordered Locus Names:YOL039W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL039W.
SGDiS000005399. RPP2A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Protein family/group databases

Allergomei9537. Sac c P2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001576811 – 10660S acidic ribosomal protein P2-alphaAdd BLAST106

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki2Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei16PhosphothreonineCombined sources1
Modified residuei40PhosphoserineCombined sources1
Modified residuei43PhosphoserineCombined sources1
Cross-linki48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei49PhosphoserineCombined sources1
Modified residuei96PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation is not involved in the interaction of the acidic P proteins with the ribosome, however it is suggested to affect the ribosome activity and to participate in a possible ribosome regulatory mechanism.
The N-terminus is not modified.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP05319.
PRIDEiP05319.
TopDownProteomicsiP05319.

PTM databases

iPTMnetiP05319.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A (PubMed:16573688, PubMed:11431471).1 Publication3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34363. 132 interactors.
DIPiDIP-2506N.
IntActiP05319. 22 interactors.
MINTiMINT-531798.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V6Ielectron microscopy8.80Bv/Bw1-106[»]
ProteinModelPortaliP05319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074828.
HOGENOMiHOG000229897.
InParanoidiP05319.
KOiK02943.
OMAiKGKNVND.
OrthoDBiEOG092C5X8C.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiView protein in InterPro
IPR027534. Ribosomal_L12.

Sequencei

Sequence statusi: Complete.

P05319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYLAAYLLL NAAGNTPDAT KIKAILESVG IEIEDEKVSS VLSALEGKSV
60 70 80 90 100
DELITEGNEK LAAVPAAGPA SAGGAAAASG DAAAEEEKEE EAAEESDDDM

GFGLFD
Length:106
Mass (Da):10,746
Last modified:November 1, 1988 - v1
Checksum:i22275AFA35E1A32E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06958 mRNA. Translation: CAA30028.1.
J03760 Genomic DNA. Translation: AAA34971.1.
M26503 Genomic DNA. Translation: AAA34735.1.
Z74781 Genomic DNA. Translation: CAA99041.1.
AY692793 Genomic DNA. Translation: AAT92812.1.
BK006948 Genomic DNA. Translation: DAA10743.1.
PIRiB28104. R5BYIB.
RefSeqiNP_014603.1. NM_001183293.1.

Genome annotation databases

EnsemblFungiiYOL039W; YOL039W; YOL039W.
GeneIDi854118.
KEGGisce:YOL039W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06958 mRNA. Translation: CAA30028.1.
J03760 Genomic DNA. Translation: AAA34971.1.
M26503 Genomic DNA. Translation: AAA34735.1.
Z74781 Genomic DNA. Translation: CAA99041.1.
AY692793 Genomic DNA. Translation: AAT92812.1.
BK006948 Genomic DNA. Translation: DAA10743.1.
PIRiB28104. R5BYIB.
RefSeqiNP_014603.1. NM_001183293.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V6Ielectron microscopy8.80Bv/Bw1-106[»]
ProteinModelPortaliP05319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34363. 132 interactors.
DIPiDIP-2506N.
IntActiP05319. 22 interactors.
MINTiMINT-531798.

Protein family/group databases

Allergomei9537. Sac c P2.

PTM databases

iPTMnetiP05319.

Proteomic databases

MaxQBiP05319.
PRIDEiP05319.
TopDownProteomicsiP05319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL039W; YOL039W; YOL039W.
GeneIDi854118.
KEGGisce:YOL039W.

Organism-specific databases

EuPathDBiFungiDB:YOL039W.
SGDiS000005399. RPP2A.

Phylogenomic databases

GeneTreeiENSGT00550000074828.
HOGENOMiHOG000229897.
InParanoidiP05319.
KOiK02943.
OMAiKGKNVND.
OrthoDBiEOG092C5X8C.

Enzyme and pathway databases

BioCyciYEAST:G3O-33453-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiPR:P05319.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiView protein in InterPro
IPR027534. Ribosomal_L12.
ProtoNetiSearch...

Entry informationi

Entry nameiRLA2_YEAST
AccessioniPrimary (citable) accession number: P05319
Secondary accession number(s): D6W227
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 10, 2017
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.