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P05318

- RLA1_YEAST

UniProt

P05318 - RLA1_YEAST

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Protein

60S acidic ribosomal protein P1-alpha

Gene
RPP1A, L12EIIA, RPA1, RPLA1, YDL081C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an important role in the elongation step of protein synthesis.UniRule annotation

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein kinase activator activity Source: SGD
  3. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. positive regulation of protein kinase activity Source: SGD
  3. translational elongation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29490-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
60S acidic ribosomal protein P1-alpha
Short name:
P1A
Alternative name(s):
A1
L12EIIA
YP1alpha
Gene namesi
Name:RPP1A
Synonyms:L12EIIA, RPA1, RPLA1
Ordered Locus Names:YDL081C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

SGDiS000002239. RPP1A.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 10610560S acidic ribosomal protein P1-alphaUniRule annotationPRO_0000157705Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications
Modified residuei96 – 961Phosphoserine2 Publications

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP05318.
PaxDbiP05318.
PeptideAtlasiP05318.

Expressioni

Gene expression databases

GenevestigatoriP05318.

Interactioni

Subunit structurei

Heterodimer of P1A-P2B. Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPP0P053174EBI-15452,EBI-15447
RPP2BP024002EBI-15452,EBI-15464

Protein-protein interaction databases

BioGridi31980. 125 interactions.
DIPiDIP-1583N.
IntActiP05318. 19 interactions.
MINTiMINT-384141.
STRINGi4932.YDL081C.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IZSelectron microscopy-t/u1-106[»]
DisProtiDP00164.
ProteinModelPortaliP05318.
SMRiP05318. Positions 3-106.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2058.
GeneTreeiENSGT00730000113211.
HOGENOMiHOG000229898.
KOiK02942.
OMAiSIESYWP.
OrthoDBiEOG7J70V7.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch.
InterProiIPR001813. Ribosomal_L10/L12.
IPR027534. Ribosomal_L12.
[Graphical view]
PfamiPF00428. Ribosomal_60s. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05318-1 [UniParc]FASTAAdd to Basket

« Hide

MSTESALSYA ALILADSEIE ISSEKLLTLT NAANVPVENI WADIFAKALD    50
GQNLKDLLVN FSAGAAAPAG VAGGVAGGEA GEAEAEKEEE EAKEESDDDM 100
GFGLFD 106
Length:106
Mass (Da):10,908
Last modified:October 5, 2010 - v4
Checksum:i9A2FE94D28640ACC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371V → D in CAA30027. 1 Publication
Sequence conflicti37 – 371V → D in CAA31976. 1 Publication
Sequence conflicti37 – 371V → D in BAA14113. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06957 mRNA. Translation: CAA30027.1.
X13682 Genomic DNA. Translation: CAA31976.1.
D90072 Genomic DNA. Translation: BAA14113.1.
M26504 Genomic DNA. Translation: AAA34733.1.
Z74129 Genomic DNA. Translation: CAA98647.1.
AY558526 Genomic DNA. Translation: AAS56852.1.
BK006938 Genomic DNA. Translation: DAA11778.1.
PIRiS67617. R5BY2A.
RefSeqiNP_010202.1. NM_001180140.1.

Genome annotation databases

EnsemblFungiiYDL081C; YDL081C; YDL081C.
GeneIDi851478.
KEGGisce:YDL081C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06957 mRNA. Translation: CAA30027.1 .
X13682 Genomic DNA. Translation: CAA31976.1 .
D90072 Genomic DNA. Translation: BAA14113.1 .
M26504 Genomic DNA. Translation: AAA34733.1 .
Z74129 Genomic DNA. Translation: CAA98647.1 .
AY558526 Genomic DNA. Translation: AAS56852.1 .
BK006938 Genomic DNA. Translation: DAA11778.1 .
PIRi S67617. R5BY2A.
RefSeqi NP_010202.1. NM_001180140.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3IZS electron microscopy - t/u 1-106 [» ]
DisProti DP00164.
ProteinModelPortali P05318.
SMRi P05318. Positions 3-106.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31980. 125 interactions.
DIPi DIP-1583N.
IntActi P05318. 19 interactions.
MINTi MINT-384141.
STRINGi 4932.YDL081C.

Proteomic databases

MaxQBi P05318.
PaxDbi P05318.
PeptideAtlasi P05318.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL081C ; YDL081C ; YDL081C .
GeneIDi 851478.
KEGGi sce:YDL081C.

Organism-specific databases

SGDi S000002239. RPP1A.

Phylogenomic databases

eggNOGi COG2058.
GeneTreei ENSGT00730000113211.
HOGENOMi HOG000229898.
KOi K02942.
OMAi SIESYWP.
OrthoDBi EOG7J70V7.

Enzyme and pathway databases

BioCyci YEAST:G3O-29490-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Miscellaneous databases

NextBioi 968786.

Gene expression databases

Genevestigatori P05318.

Family and domain databases

HAMAPi MF_01478. Ribosomal_L12_arch.
InterProi IPR001813. Ribosomal_L10/L12.
IPR027534. Ribosomal_L12.
[Graphical view ]
Pfami PF00428. Ribosomal_60s. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA and deduced amino acid sequence of acidic ribosomal protein A1 from Saccharomyces cerevisiae."
    Tsurugi K., Mitsui K.
    Nucleic Acids Res. 16:3574-3574(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: IFO 40028.
  2. "Identification of A1 protein as the fourth member of 13 kDa-type acidic ribosomal protein family in yeast Saccharomyces cerevisiae."
    Mitsui K., Tsurugi K.
    Biochem. Biophys. Res. Commun. 161:1001-1006(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: IFO 40028.
  3. "A family of genes encode the multiple forms of the Saccharomyces cerevisiae ribosomal proteins equivalent to the Escherichia coli L12 protein and a single form of the L10-equivalent ribosomal protein."
    Newton C.H., Shimmin L.C., Yee J., Dennis P.P.
    J. Bacteriol. 172:579-588(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SR26-12C.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "The acidic phosphoproteins from Saccharomyces cerevisiae ribosomes. NH2-terminal acetylation is a conserved difference between P1 and P2 proteins."
    Santos C., Ortiz-Reyes B., Naranda T., Remacha M., Ballesta J.P.G.
    Biochemistry 32:4231-4236(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-5, ACETYLATION AT SER-2, PHOSPHORYLATION.
  8. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  9. "Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins."
    Krokowski D., Boguszewska A., Abramczyk D., Liljas A., Tchorzewski M., Grankowski N.
    Mol. Microbiol. 60:386-400(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RPP0 AND RPP2B.
  10. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
  11. "Asymmetric interactions between the acidic P1 and P2 proteins in the Saccharomyces cerevisiae ribosomal stalk."
    Guarinos E., Remacha M., Ballesta J.P.G.
    J. Biol. Chem. 276:32474-32479(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RPP2B.
  12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRLA1_YEAST
AccessioniPrimary (citable) accession number: P05318
Secondary accession number(s): D6VRR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 5, 2010
Last modified: September 3, 2014
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Yeasts contain 4 individual small ribosomal A proteins (RPA) which can be classified into two couples of similar but not identical sequences. Each couple is distinctly related to one of the two A proteins present in multicellular organisms.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi