P05318 (RLA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S acidic ribosomal protein P1-alpha
Short name=P1A

Alternative name(s):
A1
L12EIIA
YP1alpha

Gene names

Name:	RPP1A
Synonyms:	L12EIIA, RPA1, RPLA1
Ordered Locus Names:	YDL081C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the elongation step of protein synthesis. HAMAP-Rule MF_01478
Subunit structure	Heterodimer of P1A-P2B. Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A. Ref.8 Ref.10 Ref.12
Subcellular location	Cytoplasm Ref.13.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA. HAMAP-Rule MF_01478
Miscellaneous	Yeasts contain 4 individual small ribosomal A proteins (RPA) which can be classified into two couples of similar but not identical sequences. Each couple is distinctly related to one of the two A proteins present in multicellular organisms. HAMAP-Rule MF_01478
Sequence similarities	Belongs to the ribosomal protein L12P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.8. Source: SGD translational elongation Inferred from sequence or structural similarity PubMed 9175862Ref.8. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.8. Source: SGD
Molecular_function	structural constituent of ribosome Traceable author statement Ref.8. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPP0	P05317	4	EBI-15452,EBI-15447
RPP2B	P02400	2	EBI-15452,EBI-15464

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7 Ref.11
	Chain	2 – 106	105	60S acidic ribosomal protein P1-alpha HAMAP-Rule MF_01478		PRO_0000157705
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.7 Ref.11
	Modified residue	96	1	Phosphoserine Ref.9 Ref.14 Ref.15
Experimental info
	Sequence conflict	37	1	V → D in CAA30027. Ref.1
	Sequence conflict	37	1	V → D in CAA31976. Ref.2
	Sequence conflict	37	1	V → D in BAA14113. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05318 [UniParc]. Last modified October 5, 2010. Version 4. Checksum: 9A2FE94D28640ACC	FASTA	106	10,908
	10 20 30 40 50 60 MSTESALSYA ALILADSEIE ISSEKLLTLT NAANVPVENI WADIFAKALD GQNLKDLLVN 70 80 90 100 FSAGAAAPAG VAGGVAGGEA GEAEAEKEEE EAKEESDDDM GFGLFD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA and deduced amino acid sequence of acidic ribosomal protein A1 from Saccharomyces cerevisiae." Tsurugi K., Mitsui K. Nucleic Acids Res. 16:3574-3574(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: IFO 40028.
[2]	"Identification of A1 protein as the fourth member of 13 kDa-type acidic ribosomal protein family in yeast Saccharomyces cerevisiae." Mitsui K., Tsurugi K. Biochem. Biophys. Res. Commun. 161:1001-1006(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IFO 40028.
[3]	"A family of genes encode the multiple forms of the Saccharomyces cerevisiae ribosomal proteins equivalent to the Escherichia coli L12 protein and a single form of the L10-equivalent ribosomal protein." Newton C.H., Shimmin L.C., Yee J., Dennis P.P. J. Bacteriol. 172:579-588(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SR26-12C.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[7]	"The acidic phosphoproteins from Saccharomyces cerevisiae ribosomes. NH2-terminal acetylation is a conserved difference between P1 and P2 proteins." Santos C., Ortiz-Reyes B., Naranda T., Remacha M., Ballesta J.P.G. Biochemistry 32:4231-4236(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-5, ACETYLATION AT SER-2, PHOSPHORYLATION.
[8]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[9]	"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae." Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M. Nat. Biotechnol. 20:301-305(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, MASS SPECTROMETRY. Strain: 2124.
[10]	"Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins." Krokowski D., Boguszewska A., Abramczyk D., Liljas A., Tchorzewski M., Grankowski N. Mol. Microbiol. 60:386-400(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RPP0 AND RPP2B.
[11]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2 BY NATA.
[12]	"Asymmetric interactions between the acidic P1 and P2 proteins in the Saccharomyces cerevisiae ribosomal stalk." Guarinos E., Remacha M., Ballesta J.P.G. J. Biol. Chem. 276:32474-32479(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RPP2B.
[13]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[14]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, MASS SPECTROMETRY. Strain: ADR376.
[15]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X06957 mRNA. Translation: CAA30027.1.
X13682 Genomic DNA. Translation: CAA31976.1.
D90072 Genomic DNA. Translation: BAA14113.1.
M26504 Genomic DNA. Translation: AAA34733.1.
Z74129 Genomic DNA. Translation: CAA98647.1.
AY558526 Genomic DNA. Translation: AAS56852.1.
BK006938 Genomic DNA. Translation: DAA11778.1.

PIR

R5BY2A. S67617.

RefSeq

NP_010202.1. NM_001180140.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IZS	electron microscopy	-	t/u	1-106	[»]

DisProt

DP00164.

ProteinModelPortal

P05318.

SMR

P05318. Positions 6-60.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1583N.

IntAct

P05318. 19 interactions.

MINT

MINT-384141.

STRING

4932.YDL081C.

Proteomic databases

PaxDb

P05318.

PeptideAtlas

P05318.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDL081C; YDL081C; YDL081C.

GeneID

851478.

KEGG

sce:YDL081C.

Organism-specific databases

SGD

S000002239. RPP1A.

Phylogenomic databases

eggNOG

COG2058.

GeneTree

ENSGT00550000074698.

HOGENOM

HOG000229898.

KO

K02942.

OMA

GIWADLF.

OrthoDB

EOG4JDMHM.

Enzyme and pathway databases

BioCyc

YEAST:G3O-29490-MONOMER.

Gene expression databases

Genevestigator

P05318.

GermOnline

YDL081C. Saccharomyces cerevisiae.

Family and domain databases

HAMAP

MF_01478. Ribosomal_L12_arch.

InterPro

IPR001813. Ribosomal_L10/L12.
IPR027534. Ribosomal_L12.
[Graphical view]

Pfam

PF00428. Ribosomal_60s. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

968786.

Entry information

Entry name

RLA1_YEAST

Accession

Primary (citable) accession number: P05318
Secondary accession number(s): D6VRR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	October 5, 2010
Last modified:	May 29, 2013
This is version 120 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program