Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

60S acidic ribosomal protein P1-alpha

Gene

RPP1A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the elongation step of protein synthesis.

GO - Molecular functioni

  • large ribosomal subunit rRNA binding Source: GO_Central
  • protein kinase activator activity Source: SGD
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • positive regulation of protein kinase activity Source: SGD
  • translational elongation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29490-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
60S acidic ribosomal protein P1-alpha
Short name:
P1A
Alternative name(s):
A1
L12EIIA
YP1alpha
Gene namesi
Name:RPP1A
Synonyms:L12EIIA, RPA1, RPLA1
Ordered Locus Names:YDL081C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL081C.
SGDiS000002239. RPP1A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD
  • preribosome, large subunit precursor Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001577052 – 10660S acidic ribosomal protein P1-alphaAdd BLAST105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine2 Publications1
Modified residuei96PhosphoserineCombined sources1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP05318.
PRIDEiP05318.

PTM databases

iPTMnetiP05318.

Interactioni

Subunit structurei

Heterodimer of P1A-P2B. Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RPP0P053174EBI-15452,EBI-15447
RPP2BP024002EBI-15452,EBI-15464

Protein-protein interaction databases

BioGridi31980. 126 interactors.
DIPiDIP-1583N.
IntActiP05318. 19 interactors.
MINTiMINT-384141.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V6Ielectron microscopy8.80Bt/Bu1-106[»]
DisProtiDP00164.
ProteinModelPortaliP05318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L12P family.Curated

Phylogenomic databases

HOGENOMiHOG000229898.
InParanoidiP05318.
KOiK02942.
OMAiHIPVEGI.
OrthoDBiEOG092C5X8C.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiIPR027534. Ribosomal_L12.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05318-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTESALSYA ALILADSEIE ISSEKLLTLT NAANVPVENI WADIFAKALD
60 70 80 90 100
GQNLKDLLVN FSAGAAAPAG VAGGVAGGEA GEAEAEKEEE EAKEESDDDM

GFGLFD
Length:106
Mass (Da):10,908
Last modified:October 5, 2010 - v4
Checksum:i9A2FE94D28640ACC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37V → D in CAA30027 (PubMed:3287328).Curated1
Sequence conflicti37V → D in CAA31976 (PubMed:2662969).Curated1
Sequence conflicti37V → D in BAA14113 (PubMed:2662969).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06957 mRNA. Translation: CAA30027.1.
X13682 Genomic DNA. Translation: CAA31976.1.
D90072 Genomic DNA. Translation: BAA14113.1.
M26504 Genomic DNA. Translation: AAA34733.1.
Z74129 Genomic DNA. Translation: CAA98647.1.
AY558526 Genomic DNA. Translation: AAS56852.1.
BK006938 Genomic DNA. Translation: DAA11778.1.
PIRiS67617. R5BY2A.
RefSeqiNP_010202.1. NM_001180140.1.

Genome annotation databases

EnsemblFungiiYDL081C; YDL081C; YDL081C.
GeneIDi851478.
KEGGisce:YDL081C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06957 mRNA. Translation: CAA30027.1.
X13682 Genomic DNA. Translation: CAA31976.1.
D90072 Genomic DNA. Translation: BAA14113.1.
M26504 Genomic DNA. Translation: AAA34733.1.
Z74129 Genomic DNA. Translation: CAA98647.1.
AY558526 Genomic DNA. Translation: AAS56852.1.
BK006938 Genomic DNA. Translation: DAA11778.1.
PIRiS67617. R5BY2A.
RefSeqiNP_010202.1. NM_001180140.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V6Ielectron microscopy8.80Bt/Bu1-106[»]
DisProtiDP00164.
ProteinModelPortaliP05318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31980. 126 interactors.
DIPiDIP-1583N.
IntActiP05318. 19 interactors.
MINTiMINT-384141.

PTM databases

iPTMnetiP05318.

Proteomic databases

MaxQBiP05318.
PRIDEiP05318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL081C; YDL081C; YDL081C.
GeneIDi851478.
KEGGisce:YDL081C.

Organism-specific databases

EuPathDBiFungiDB:YDL081C.
SGDiS000002239. RPP1A.

Phylogenomic databases

HOGENOMiHOG000229898.
InParanoidiP05318.
KOiK02942.
OMAiHIPVEGI.
OrthoDBiEOG092C5X8C.

Enzyme and pathway databases

BioCyciYEAST:G3O-29490-MONOMER.

Miscellaneous databases

PROiP05318.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiIPR027534. Ribosomal_L12.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRLA1_YEAST
AccessioniPrimary (citable) accession number: P05318
Secondary accession number(s): D6VRR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 151 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Yeasts contain 4 individual small ribosomal A proteins (RPA) which can be classified into two couples of similar but not identical sequences. Each couple is distinctly related to one of the two A proteins present in multicellular organisms.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.