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Protein

60S acidic ribosomal protein P1-alpha

Gene

RPP1A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

The 4 small acidic ribosomal P-proteins from yeast can be classified into two couples of similar but not identical sequences. Each couple (P1A/P1B and P2A/P2B) is distinctly related to one of the two acidic ribosomal P-proteins P1/P2 present in multicellular organisms.1 Publication

GO - Molecular functioni

  • protein kinase activator activity Source: SGD
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • positive regulation of protein kinase activity Source: SGD
  • translational elongation Source: InterPro

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-29490-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
60S acidic ribosomal protein P1-alpha1 Publication
Short name:
A1
Alternative name(s):
L12eIIA
Large ribosomal subunit protein P1-A1 Publication
Short name:
P1A
YP1alpha
Gene namesi
Name:RPP1A1 Publication
Synonyms:L12EIIA, RPA1, RPLA1
Ordered Locus Names:YDL081C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL081C.
SGDiS000002239. RPP1A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001577052 – 10660S acidic ribosomal protein P1-alphaAdd BLAST105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine2 Publications1
Modified residuei96PhosphoserineCombined sources1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP05318.
PRIDEiP05318.

PTM databases

iPTMnetiP05318.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which uL10/P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A (PubMed:16573688, PubMed:11431471).1 Publication3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31980. 383 interactors.
DIPiDIP-1583N.
IntActiP05318. 19 interactors.
MINTiMINT-384141.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V6Ielectron microscopy8.80Bt/Bu1-106[»]
5DGEX-ray3.45p1/p21-106[»]
DisProtiDP00164.
ProteinModelPortaliP05318.
SMRiP05318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000229898.
InParanoidiP05318.
KOiK02942.
OMAiHIPVEGI.
OrthoDBiEOG092C5X8C.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiView protein in InterPro
IPR027534. Ribosomal_L12.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05318-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTESALSYA ALILADSEIE ISSEKLLTLT NAANVPVENI WADIFAKALD
60 70 80 90 100
GQNLKDLLVN FSAGAAAPAG VAGGVAGGEA GEAEAEKEEE EAKEESDDDM

GFGLFD
Length:106
Mass (Da):10,908
Last modified:October 5, 2010 - v4
Checksum:i9A2FE94D28640ACC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37V → D in CAA30027 (PubMed:3287328).Curated1
Sequence conflicti37V → D in CAA31976 (PubMed:2662969).Curated1
Sequence conflicti37V → D in BAA14113 (PubMed:2662969).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06957 mRNA. Translation: CAA30027.1.
X13682 Genomic DNA. Translation: CAA31976.1.
D90072 Genomic DNA. Translation: BAA14113.1.
M26504 Genomic DNA. Translation: AAA34733.1.
Z74129 Genomic DNA. Translation: CAA98647.1.
AY558526 Genomic DNA. Translation: AAS56852.1.
BK006938 Genomic DNA. Translation: DAA11778.1.
PIRiS67617. R5BY2A.
RefSeqiNP_010202.1. NM_001180140.1.

Genome annotation databases

EnsemblFungiiYDL081C; YDL081C; YDL081C.
GeneIDi851478.
KEGGisce:YDL081C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06957 mRNA. Translation: CAA30027.1.
X13682 Genomic DNA. Translation: CAA31976.1.
D90072 Genomic DNA. Translation: BAA14113.1.
M26504 Genomic DNA. Translation: AAA34733.1.
Z74129 Genomic DNA. Translation: CAA98647.1.
AY558526 Genomic DNA. Translation: AAS56852.1.
BK006938 Genomic DNA. Translation: DAA11778.1.
PIRiS67617. R5BY2A.
RefSeqiNP_010202.1. NM_001180140.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4V6Ielectron microscopy8.80Bt/Bu1-106[»]
5DGEX-ray3.45p1/p21-106[»]
DisProtiDP00164.
ProteinModelPortaliP05318.
SMRiP05318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31980. 383 interactors.
DIPiDIP-1583N.
IntActiP05318. 19 interactors.
MINTiMINT-384141.

PTM databases

iPTMnetiP05318.

Proteomic databases

MaxQBiP05318.
PRIDEiP05318.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL081C; YDL081C; YDL081C.
GeneIDi851478.
KEGGisce:YDL081C.

Organism-specific databases

EuPathDBiFungiDB:YDL081C.
SGDiS000002239. RPP1A.

Phylogenomic databases

HOGENOMiHOG000229898.
InParanoidiP05318.
KOiK02942.
OMAiHIPVEGI.
OrthoDBiEOG092C5X8C.

Enzyme and pathway databases

BioCyciYEAST:G3O-29490-MONOMER.

Miscellaneous databases

PROiPR:P05318.

Family and domain databases

HAMAPiMF_01478. Ribosomal_L12_arch. 1 hit.
InterProiView protein in InterPro
IPR027534. Ribosomal_L12.
ProtoNetiSearch...

Entry informationi

Entry nameiRLA1_YEAST
AccessioniPrimary (citable) accession number: P05318
Secondary accession number(s): D6VRR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 5, 2010
Last modified: April 12, 2017
This is version 154 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.