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P05317 (RLA0_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 138. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
60S acidic ribosomal protein P0

Short name=A0
Alternative name(s):
L10E
Gene names
Name:RPP0
Synonyms:L10E, RPA0, RPL10E, RPLA0
Ordered Locus Names:YLR340W
ORF Names:L8300.8
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ribosomal protein P0 is the functional equivalent of E.coli protein L10.

Subunit structure

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). The 5 acidic ribosomal P-proteins form the stalk structure of the 60S subunit. They are organized as a pentameric complex in which P0 interacts with 2 heterodimers, P1A-P2B and P1B-P2A. P0 directly interacts with 28S rRNA. Interacts with YFL034W. Ref.7 Ref.9 Ref.10

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the ribosomal protein L10P family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 31231260S acidic ribosomal protein P0
PRO_0000154786

Regions

Region199 – 23032Interaction with P1A-P2B
Region231 – 25828Interaction with P1B-P2A

Amino acid modifications

Modified residue681Phosphoserine Ref.13
Modified residue3021Phosphoserine; by CK2 Ref.8 Ref.11 Ref.12 Ref.13

Experimental info

Sequence conflict831N → Y in CAA30029. Ref.1
Sequence conflict831N → Y in BAA00415. Ref.2
Sequence conflict831N → Y in CAA31703. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P05317 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: 109ECC90F7C8B68E

FASTA31233,717
        10         20         30         40         50         60 
MGGIREKKAE YFAKLREYLE EYKSLFVVGV DNVSSQQMHE VRKELRGRAV VLMGKNTMVR 

        70         80         90        100        110        120 
RAIRGFLSDL PDFEKLLPFV KGNVGFVFTN EPLTEIKNVI VSNRVAAPAR AGAVAPEDIW 

       130        140        150        160        170        180 
VRAVNTGMEP GKTSFFQALG VPTKIARGTI EIVSDVKVVD AGNKVGQSEA SLLNLLNISP 

       190        200        210        220        230        240 
FTFGLTVVQV YDNGQVFPSS ILDITDEELV SHFVSAVSTI ASISLAIGYP TLPSVGHTLI 

       250        260        270        280        290        300 
NNYKDLLAVA IAASYHYPEI EDLVDRIENP EKYAAAAPAA TSAASGDAAP AEEAAAEEEE 

       310 
ESDDDMGFGL FD 

« Hide

References

« Hide 'large scale' references
[1]"cDNA and deduced amino acid sequence of 38 kDa-type acidic ribosomal protein A0 from Saccharomyces cerevisiae."
Mitsui K., Tsurugi K.
Nucleic Acids Res. 16:3573-3573(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: IFO 40028.
[2]"The gene and the primary structure of acidic ribosomal protein A0 from yeast Saccharomyces cerevisiae which shows partial homology to bacterial ribosomal protein L10."
Mitsui K., Nakagawa T., Tsurugi K.
J. Biochem. 106:223-227(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A family of genes encode the multiple forms of the Saccharomyces cerevisiae ribosomal proteins equivalent to the Escherichia coli L12 protein and a single form of the L10-equivalent ribosomal protein."
Newton C.H., Shimmin L.C., Yee J., Dennis P.P.
J. Bacteriol. 172:579-588(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SR26-12C.
[4]Erratum
Newton C.H., Shimmin L.C., Yee J., Dennis P.P.
J. Bacteriol. 172:3535-3535(1990) [PubMed] [Europe PMC] [Abstract]
[5]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[8]"Phosphorylation of ribosomal protein P0 is not essential for ribosome function but can affect translation."
Rodriguez-Gabriel M.A., Remacha M., Ballesta J.P.G.
Biochemistry 37:16620-16626(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-302.
[9]"Identification of a hypothetical membrane protein interactor of ribosomal phosphoprotein P0."
Aruna K., Chakraborty T., Nambeesan S., Mannan A.B., Sehgal A., Balachandara S.R., Sharma S.
J. Biosci. 29:33-43(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YFL034W.
[10]"Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins."
Krokowski D., Boguszewska A., Abramczyk D., Liljas A., Tchorzewski M., Grankowski N.
Mol. Microbiol. 60:386-400(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RPP1A; RPP1B; RPP2A AND RPP2B.
[11]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68 AND SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X06959 mRNA. Translation: CAA30029.1.
D00529 Genomic DNA. Translation: BAA00415.1.
X13328 Genomic DNA. Translation: CAA31703.1.
M26506 Genomic DNA. Translation: AAA34730.1.
M37326 Genomic DNA. Translation: AAA34729.1.
U19028 Genomic DNA. Translation: AAB67258.1.
BK006945 Genomic DNA. Translation: DAA09644.1.
PIRR5BY0E. S51343.
RefSeqNP_013444.1. NM_001182229.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3IZSelectron microscopy-s1-312[»]
3J16electron microscopy-G1-312[»]
3O5HX-ray4.00M1-312[»]
3U5IX-ray3.00q1-312[»]
4B6Aelectron microscopy8.10q1-312[»]
4BYNelectron microscopy4.30q1-312[»]
4BYUelectron microscopy6.60q1-237[»]
ProteinModelPortalP05317.
SMRP05317. Positions 3-270.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31602. 149 interactions.
DIPDIP-1582N.
IntActP05317. 267 interactions.
MINTMINT-384116.
STRING4932.YLR340W.

2D gel databases

SWISS-2DPAGEP05317.

Proteomic databases

MaxQBP05317.
PaxDbP05317.
PeptideAtlasP05317.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR340W; YLR340W; YLR340W.
GeneID851052.
KEGGsce:YLR340W.

Organism-specific databases

SGDS000004332. RPP0.

Phylogenomic databases

eggNOGCOG0244.
GeneTreeENSGT00390000017839.
HOGENOMHOG000210987.
KOK02941.
OMALATEYSF.
OrthoDBEOG7PS1RV.

Enzyme and pathway databases

BioCycYEAST:G3O-32417-MONOMER.

Gene expression databases

GenevestigatorP05317.

Family and domain databases

InterProIPR001790. Ribosomal_L10/acidic_P0.
IPR001813. Ribosomal_L10/L12.
[Graphical view]
PfamPF00428. Ribosomal_60s. 1 hit.
PF00466. Ribosomal_L10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio967667.

Entry information

Entry nameRLA0_YEAST
AccessionPrimary (citable) accession number: P05317
Secondary accession number(s): D6VYX8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references