ID   FUR4_YEAST              Reviewed;         633 AA.
AC   P05316; D6VQ23;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 2.
DT   27-MAR-2024, entry version 168.
DE   RecName: Full=Uracil permease;
GN   Name=FUR4; OrderedLocusNames=YBR021W; ORFNames=YBR0303;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RX   PubMed=3276521; DOI=10.1111/j.1432-1033.1988.tb13806.x;
RA   Jund R., Weber E., Chevallier M.-R.;
RT   "Primary structure of the uracil transport protein of Saccharomyces
RT   cerevisiae.";
RL   Eur. J. Biochem. 171:417-424(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8091864; DOI=10.1002/yea.320100010;
RA   Smits P.H.M., de Haan M., Maat C., Grivell L.A.;
RT   "The complete sequence of a 33 kb fragment on the right arm of chromosome
RT   II from Saccharomyces cerevisiae reveals 16 open reading frames, including
RT   ten new open reading frames, five previously identified genes and a
RT   homologue of the SCO1 gene.";
RL   Yeast 10:S75-S80(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
CC   -!- FUNCTION: Transport of uracil.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- PTM: Glycosylated (possible); but there is not yet direct biochemical
CC       evidence for it.
CC   -!- SIMILARITY: Belongs to the purine-cytosine permease (2.A.39) family.
CC       {ECO:0000305}.
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DR   EMBL; X76078; CAA53678.1; -; Genomic_DNA.
DR   EMBL; Z35890; CAA84963.1; -; Genomic_DNA.
DR   EMBL; X06830; CAA29986.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07143.1; -; Genomic_DNA.
DR   PIR; S45877; S45877.
DR   RefSeq; NP_009577.1; NM_001178369.1.
DR   AlphaFoldDB; P05316; -.
DR   SMR; P05316; -.
DR   BioGRID; 32724; 22.
DR   DIP; DIP-4892N; -.
DR   IntAct; P05316; 32.
DR   STRING; 4932.YBR021W; -.
DR   TCDB; 2.A.39.3.2; the nucleobase:cation symporter-1 (ncs1) family.
DR   iPTMnet; P05316; -.
DR   MaxQB; P05316; -.
DR   PaxDb; 4932-YBR021W; -.
DR   PeptideAtlas; P05316; -.
DR   EnsemblFungi; YBR021W_mRNA; YBR021W; YBR021W.
DR   GeneID; 852309; -.
DR   KEGG; sce:YBR021W; -.
DR   AGR; SGD:S000000225; -.
DR   SGD; S000000225; FUR4.
DR   VEuPathDB; FungiDB:YBR021W; -.
DR   eggNOG; KOG2466; Eukaryota.
DR   GeneTree; ENSGT00940000176299; -.
DR   HOGENOM; CLU_021555_2_2_1; -.
DR   InParanoid; P05316; -.
DR   OMA; VKSIMNC; -.
DR   OrthoDB; 1218881at2759; -.
DR   BioCyc; YEAST:G3O-29002-MONOMER; -.
DR   BioGRID-ORCS; 852309; 5 hits in 10 CRISPR screens.
DR   PRO; PR:P05316; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P05316; Protein.
DR   GO; GO:0071944; C:cell periphery; HDA:SGD.
DR   GO; GO:0032126; C:eisosome; IDA:SGD.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   GO; GO:0015205; F:nucleobase transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015505; F:uracil:monoatomic cation symporter activity; IDA:SGD.
DR   GO; GO:0015851; P:nucleobase transport; IBA:GO_Central.
DR   GO; GO:0055085; P:transmembrane transport; IDA:SGD.
DR   GO; GO:0015857; P:uracil transport; IDA:SGD.
DR   CDD; cd11482; SLC-NCS1sbd_NRT1-like; 1.
DR   Gene3D; 1.10.4160.10; Hydantoin permease; 1.
DR   InterPro; IPR012681; NCS1.
DR   InterPro; IPR001248; Pur-cyt_permease.
DR   InterPro; IPR045225; Uracil/uridine/allantoin_perm.
DR   NCBIfam; TIGR00800; ncs1; 1.
DR   PANTHER; PTHR30618:SF2; ALLANTOIN PERMEASE-RELATED; 1.
DR   PANTHER; PTHR30618; NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTER; 1.
DR   Pfam; PF02133; Transp_cyt_pur; 1.
PE   3: Inferred from homology;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..633
FT                   /note="Uracil permease"
FT                   /id="PRO_0000197925"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        173..193
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        268..288
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        310..330
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        400..420
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        442..462
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        465..485
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        521..541
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        559..579
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        217
FT                   /note="M -> I (in Ref. 1; CAA29986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        229
FT                   /note="K -> R (in Ref. 1; CAA29986)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        320
FT                   /note="G -> R (in Ref. 1; CAA29986)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   633 AA;  71736 MW;  2FFCD9529412B2D6 CRC64;
     MPDNLSLHLS GSSKRLNSRQ LMESSNETFA PNNVDLEKEY KSSQSNITTE VYEASSFEEK
     VSSEKPQYSS FWKKIYYEYV VVDKSILGVS ILDSFMYNQD LKPVEKERRV WSWYNYCYFW
     LAECFNINTW QIAATGLQLG LNWWQCWITI WIGYGFVGAF VVLASRVGSA YHLSFPISSR
     ASFGIFFSLW PVINRVVMAI VWYSVQAYIA ATPVSLMLKS IFGKDLQDKI PDHFGSPNAT
     TYEFMCFFIF WAASLPFLLV PPHKIRHLFT VKAVLVPFAS FGFLIWAIRR AHGRIALGSL
     TDVQPHGSAF SWAFLRSLMG CMANFSTMVI NAPDFSRFSK NPNSALWSQL VCIPFLFSIT
     CLIGILVTAA GYEIYGINYW SPLDVLEKFL QTTYNKGTRA GVFLISFVFA VAQLGTNISA
     NSLSCGTDMS AIFPKFINIK RGSLFCAAMA LCICPWNLMA TSSKFTMALS AYAIFLSSIA
     GVVCSDYFVV RRGYIKLTHI YSHQKGSFYM YGNRFGINWR ALAAYLCGVA PCLPGFIAEV
     GAPAIKVSDG AMKLYYLSYW VGYGLSFSSY TALCYFFPVP GCPVNNIIKD KGWFQRWANV
     DDFEEEWKDT IERDDLVDDN ISVYEHEHEK TFI
//