P05316 (FUR4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil permease

Gene names

Name:	FUR4
Ordered Locus Names:	YBR021W
ORF Names:	YBR0303

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	633 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transport of uracil.
Subcellular location	Membrane; Multi-pass membrane protein.
Post-translational modification	Glycosylated (possible); but there is not yet direct biochemical evidence for it.
Sequence similarities	Belongs to the purine-cytosine permease family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD membrane raft Inferred from direct assay PubMed 15536122. Source: SGD plasma membrane Inferred from direct assay PubMed 8948441. Source: SGD
Molecular_function	uracil:cation symporter activity Inferred from direct assay PubMed 8948441. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 633	633	Uracil permease		PRO_0000197925
Regions
	Transmembrane	143 – 163	21	Helical; Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Transmembrane	197 – 217	21	Helical; Potential
	Transmembrane	242 – 262	21	Helical; Potential
	Transmembrane	268 – 288	21	Helical; Potential
	Transmembrane	310 – 330	21	Helical; Potential
	Transmembrane	350 – 370	21	Helical; Potential
	Transmembrane	400 – 420	21	Helical; Potential
	Transmembrane	442 – 462	21	Helical; Potential
	Transmembrane	465 – 485	21	Helical; Potential
	Transmembrane	521 – 541	21	Helical; Potential
	Transmembrane	559 – 579	21	Helical; Potential
Amino acid modifications
	Modified residue	6	1	Phosphoserine Ref.5
	Modified residue	622	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	217	1	M → I in CAA29986. Ref.1
	Sequence conflict	229	1	K → R in CAA29986. Ref.1
	Sequence conflict	320	1	G → R in CAA29986. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P05316 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 2FFCD9529412B2D6	FASTA	633	71,736
	10 20 30 40 50 60 MPDNLSLHLS GSSKRLNSRQ LMESSNETFA PNNVDLEKEY KSSQSNITTE VYEASSFEEK 70 80 90 100 110 120 VSSEKPQYSS FWKKIYYEYV VVDKSILGVS ILDSFMYNQD LKPVEKERRV WSWYNYCYFW 130 140 150 160 170 180 LAECFNINTW QIAATGLQLG LNWWQCWITI WIGYGFVGAF VVLASRVGSA YHLSFPISSR 190 200 210 220 230 240 ASFGIFFSLW PVINRVVMAI VWYSVQAYIA ATPVSLMLKS IFGKDLQDKI PDHFGSPNAT 250 260 270 280 290 300 TYEFMCFFIF WAASLPFLLV PPHKIRHLFT VKAVLVPFAS FGFLIWAIRR AHGRIALGSL 310 320 330 340 350 360 TDVQPHGSAF SWAFLRSLMG CMANFSTMVI NAPDFSRFSK NPNSALWSQL VCIPFLFSIT 370 380 390 400 410 420 CLIGILVTAA GYEIYGINYW SPLDVLEKFL QTTYNKGTRA GVFLISFVFA VAQLGTNISA 430 440 450 460 470 480 NSLSCGTDMS AIFPKFINIK RGSLFCAAMA LCICPWNLMA TSSKFTMALS AYAIFLSSIA 490 500 510 520 530 540 GVVCSDYFVV RRGYIKLTHI YSHQKGSFYM YGNRFGINWR ALAAYLCGVA PCLPGFIAEV 550 560 570 580 590 600 GAPAIKVSDG AMKLYYLSYW VGYGLSFSSY TALCYFFPVP GCPVNNIIKD KGWFQRWANV 610 620 630 DDFEEEWKDT IERDDLVDDN ISVYEHEHEK TFI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the uracil transport protein of Saccharomyces cerevisiae." Jund R., Weber E., Chevallier M.-R. Eur. J. Biochem. 171:417-424(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene." Smits P.H.M., de Haan M., Maat C., Grivell L.A. Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6 AND SER-622, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76078 Genomic DNA. Translation: CAA53678.1. Z35890 Genomic DNA. Translation: CAA84963.1. X06830 Genomic DNA. Translation: CAA29986.1. BK006936 Genomic DNA. Translation: DAA07143.1.
PIR	S45877.
RefSeq	NP_009577.1. NM_001178369.1.
3D structure databases
ProteinModelPortal	P05316.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4892N.
IntAct	P05316. 31 interactions.
MINT	MINT-485049.
STRING	4932.YBR021W.
Protein family/group databases
TCDB	2.A.39.3.2. nucleobase:cation symporter-1 (NCS1) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR021W; YBR021W; YBR021W.
GeneID	852309.
KEGG	sce:YBR021W.
Organism-specific databases
CYGD	YBR021w.
SGD	S000000225. FUR4.
Phylogenomic databases
eggNOG	COG1953.
GeneTree	ENSGT00530000065049.
HOGENOM	HOG000202588.
OMA	TPASLDC.
OrthoDB	EOG4XPTQ8.
Gene expression databases
Genevestigator	P05316.
GermOnline	YBR021W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001248. Cyt_pur_permease. IPR012681. NCS1. [Graphical view]
Pfam	PF02133. Transp_cyt_pur. 1 hit. [Graphical view]
TIGRFAMs	TIGR00800. ncs1. 1 hit.
ProtoNet	Search...
Other
NextBio	970987.

Entry information

Entry name

FUR4_YEAST

Accession

Primary (citable) accession number: P05316
Secondary accession number(s): D6VQ23

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program