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P05311 (PSAB_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Photosystem I P700 chlorophyll a apoprotein A2

EC=1.97.1.12
Alternative name(s):
PSI-B
PsaB
Gene names
Name:psaB
Synonyms:psaA2
Encoded onPlastid; Chloroplast
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length734 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin By similarity. HAMAP-Rule MF_00482

Catalytic activity

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin. HAMAP-Rule MF_00482

Cofactor

P700 is a chlorophyll a/chlorophyll a' dimer, A0 is one or more chlorophyll a, A1 is one or both phylloquinones and FX is a shared 4Fe-4S iron-sulfur center By similarity.

Subunit structure

The PsaA/B heterodimer binds the P700 chlorophyll special pair and subsequent electron acceptors. PSI consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The eukaryotic PSI reaction center is composed of at least 11 subunits By similarity.

Subcellular location

Plastidchloroplast thylakoid membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00482.

Sequence similarities

Belongs to the PsaA/PsaB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 734734Photosystem I P700 chlorophyll a apoprotein A2 HAMAP-Rule MF_00482
PRO_0000088630

Regions

Transmembrane46 – 6924Helical; Name=I; Potential
Transmembrane135 – 15824Helical; Name=II; Potential
Transmembrane175 – 19925Helical; Name=III; Potential
Transmembrane273 – 29119Helical; Name=IV; Potential
Transmembrane330 – 35324Helical; Name=V; Potential
Transmembrane369 – 39527Helical; Name=VI; Potential
Transmembrane417 – 43923Helical; Name=VII; Potential
Transmembrane517 – 53519Helical; Name=VIII; Potential
Transmembrane575 – 59622Helical; Name=IX; Potential
Transmembrane643 – 66523Helical; Name=X; Potential
Transmembrane707 – 72721Helical; Name=XI; Potential

Sites

Metal binding5591Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
Metal binding5681Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
Metal binding6541Magnesium (chlorophyll-a B1 axial ligand; P700 special pair) By similarity
Metal binding6621Magnesium (chlorophyll-a B3 axial ligand) By similarity
Binding site6701Chlorophyll-a B3 By similarity
Binding site6711Phylloquinone B By similarity

Secondary structure

...................................................................................................................................................... 734
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P05311 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 2ACF1F77D46FA8E7

FASTA73482,412
        10         20         30         40         50         60 
MALRIPRFSQ GIAQDPTTRR IWFGIATAHD FESHDDITEG RLYQNIFASH FGQLAIIFLW 

        70         80         90        100        110        120 
TSGNLFHVAW QGNFEAWVQD PFHVRPIAHA IWDPHFGQPA VEAFTRGGAL GPVNNAYSGV 

       130        140        150        160        170        180 
YQWWYTIGLR TNEDLYTGAI FLLFLSFISL LAGWLHLQPK WKPSVSWFKN AESRLNHHLS 

       190        200        210        220        230        240 
GLFGVSSLAW AGHLVHVAIP GSRGEYVRWN NFLDVLPYPQ GLGPLLTGQW NLYAQNPSSS 

       250        260        270        280        290        300 
NHLFGTTQGA GTAILTILGG FHPQTQSLWL TDVAHHHLAI AFLFLIGGLM YRTNFGIGHS 

       310        320        330        340        350        360 
IKYILEAHIP PGGRLGRGHK GLYDTINNSI HFQLGLALAS LGVITSLVAQ HMYSLPAYAF 

       370        380        390        400        410        420 
IAQDFTTQAA LYTHHQYIAG FIMTGAFAHG PIFFIRDYNP EQNADNVLAR MLEHKEAIIS 

       430        440        450        460        470        480 
HLSWASLFLG FHTLGLYVHN DVMLAFGTPE KQILIEPIFA QWIQSAHGKT TYGFDIPLSS 

       490        500        510        520        530        540 
TNGPALNAGR NIWLPGWLNA INENSNSLFL TIGPGDFLVH HAIALGLHTT TLILVKGALD 

       550        560        570        580        590        600 
ARGSKLMPDK KDFGYSFPCD GPGRGGTCDI SAWDDFYLAV FWMLNTIGWV TFYWHWKHIT 

       610        620        630        640        650        660 
LWRGNVSQFN ESSTYLMGWL RDYLWLNSSQ LINGITPLVC NSLSVWAWMF LFGHLVWATG 

       670        680        690        700        710        720 
FMFLISWRGY WQELIETLAW AHERTPLANL IRWRDKPVAL SIVQARLVGL VHFSVGYIFT 

       730 
YAAFLIASTS GKFG 

« Hide

References

[1]"Sequence of two genes in pea chloroplast DNA coding for 84 and 82 kD polypeptides of the photosystem I complex."
Lehmbeck J., Rasmussen O.F., Bookjans G.B., Jepsen B.R., Stummann B.M., Henningsen K.W.
Plant Mol. Biol. 7:3-10(1986) [AGRICOLA] [Europe PMC]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05423 Genomic DNA. Translation: CAA29004.1.
PIRS00704.
RefSeqYP_003587537.1. NC_014057.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YO9model-B1-734[»]
2O01X-ray3.40B2-733[»]
2WSCX-ray3.30B1-734[»]
2WSEX-ray3.49B1-734[»]
2WSFX-ray3.48B1-734[»]
3LW5X-ray3.30B2-734[»]
ProteinModelPortalP05311.
SMRP05311. Positions 2-733.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60282N.

Proteomic databases

PRIDEP05311.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9073070.

Family and domain databases

Gene3D1.20.1130.10. 1 hit.
HAMAPMF_00482. PSI_PsaB.
InterProIPR001280. PSI_PsaA/B.
IPR020586. PSI_PsaA/B_CS.
IPR006244. PSI_PsaB.
[Graphical view]
PfamPF00223. PsaA_PsaB. 1 hit.
[Graphical view]
PIRSFPIRSF002905. PSI_A. 1 hit.
PRINTSPR00257. PHOTSYSPSAAB.
SUPFAMSSF81558. SSF81558. 1 hit.
TIGRFAMsTIGR01336. psaB. 1 hit.
PROSITEPS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP05311.

Entry information

Entry namePSAB_PEA
AccessionPrimary (citable) accession number: P05311
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: May 14, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references