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P05311

- PSAB_PEA

UniProt

P05311 - PSAB_PEA

Protein

Photosystem I P700 chlorophyll a apoprotein A2

Gene

psaB

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin.UniRule annotation

    Catalytic activityi

    Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin.UniRule annotation

    Cofactori

    P700 is a chlorophyll a/chlorophyll a' dimer, A0 is one or more chlorophyll a, A1 is one or both phylloquinones and FX is a shared 4Fe-4S iron-sulfur center.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi559 – 5591Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
    Metal bindingi568 – 5681Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
    Metal bindingi654 – 6541Magnesium (chlorophyll-a B1 axial ligand; P700 special pair)UniRule annotation
    Metal bindingi662 – 6621Magnesium (chlorophyll-a B3 axial ligand)UniRule annotation
    Binding sitei670 – 6701Chlorophyll-a B3UniRule annotation
    Binding sitei671 – 6711Phylloquinone BUniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. chlorophyll binding Source: UniProtKB-KW
    3. electron carrier activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. oxidoreductase activity Source: UniProtKB-KW

    GO - Biological processi

    1. photosynthesis Source: UniProtKB-HAMAP
    2. protein-chromophore linkage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    4Fe-4S, Chlorophyll, Chromophore, Iron, Iron-sulfur, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Photosystem I P700 chlorophyll a apoprotein A2UniRule annotation (EC:1.97.1.12UniRule annotation)
    Alternative name(s):
    PSI-BUniRule annotation
    PsaBUniRule annotation
    Gene namesi
    Name:psaBUniRule annotation
    Synonyms:psaA2
    Encoded oniPlastid; Chloroplast
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    Plastidchloroplast thylakoid membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

    GO - Cellular componenti

    1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. photosystem I Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Membrane, Photosystem I, Plastid, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 734734Photosystem I P700 chlorophyll a apoprotein A2PRO_0000088630Add
    BLAST

    Proteomic databases

    PRIDEiP05311.

    Interactioni

    Subunit structurei

    The PsaA/B heterodimer binds the P700 chlorophyll special pair and subsequent electron acceptors. PSI consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The eukaryotic PSI reaction center is composed of at least 11 subunits.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-60282N.

    Structurei

    Secondary structure

    1
    734
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 83
    Helixi12 – 143
    Helixi19 – 224
    Turni23 – 264
    Beta strandi32 – 365
    Helixi39 – 479
    Turni48 – 514
    Helixi52 – 6413
    Beta strandi67 – 748
    Turni75 – 784
    Beta strandi82 – 843
    Beta strandi101 – 1055
    Beta strandi109 – 1113
    Helixi120 – 1223
    Turni123 – 1264
    Helixi139 – 1468
    Helixi148 – 1558
    Beta strandi158 – 1614
    Helixi166 – 1683
    Turni173 – 1753
    Helixi178 – 1814
    Helixi183 – 19614
    Helixi199 – 2024
    Beta strandi212 – 2143
    Beta strandi217 – 2204
    Helixi224 – 2263
    Turni230 – 2334
    Beta strandi234 – 2363
    Beta strandi244 – 2496
    Turni263 – 2653
    Turni270 – 2745
    Helixi275 – 28713
    Beta strandi288 – 2914
    Beta strandi294 – 2963
    Beta strandi301 – 3055
    Beta strandi313 – 3164
    Helixi322 – 3265
    Turni327 – 3293
    Helixi333 – 3364
    Turni338 – 3403
    Helixi341 – 35111
    Turni352 – 3543
    Beta strandi358 – 3603
    Beta strandi362 – 3643
    Turni367 – 3693
    Beta strandi370 – 3734
    Turni374 – 3807
    Helixi381 – 3899
    Turni390 – 3967
    Turni400 – 4034
    Turni407 – 4148
    Helixi415 – 4184
    Turni419 – 4224
    Helixi423 – 44321
    Turni444 – 4474
    Helixi458 – 4625
    Turni463 – 4686
    Turni477 – 4815
    Beta strandi482 – 4854
    Beta strandi487 – 4893
    Beta strandi490 – 4967
    Beta strandi501 – 5044
    Helixi516 – 5227
    Turni523 – 5286
    Helixi529 – 53810
    Helixi540 – 5423
    Beta strandi547 – 5493
    Helixi550 – 5534
    Helixi563 – 5653
    Helixi573 – 5797
    Turni580 – 5889
    Helixi589 – 5935
    Beta strandi594 – 5996
    Beta strandi602 – 6043
    Helixi607 – 6115
    Beta strandi614 – 6163
    Turni617 – 6215
    Turni623 – 6253
    Helixi629 – 6324
    Turni633 – 6353
    Helixi636 – 6383
    Helixi645 – 65814
    Helixi662 – 6654
    Helixi669 – 67810
    Helixi682 – 6843
    Beta strandi688 – 6903
    Beta strandi694 – 6963
    Helixi704 – 7085
    Beta strandi709 – 7124
    Helixi713 – 72614
    Beta strandi729 – 7313

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YO9model-B1-734[»]
    2O01X-ray3.40B2-733[»]
    2WSCX-ray3.30B1-734[»]
    2WSEX-ray3.49B1-734[»]
    2WSFX-ray3.48B1-734[»]
    3LW5X-ray3.30B2-734[»]
    ProteinModelPortaliP05311.
    SMRiP05311. Positions 2-733.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05311.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei46 – 6924Helical; Name=IUniRule annotationAdd
    BLAST
    Transmembranei135 – 15824Helical; Name=IIUniRule annotationAdd
    BLAST
    Transmembranei175 – 19925Helical; Name=IIIUniRule annotationAdd
    BLAST
    Transmembranei273 – 29119Helical; Name=IVUniRule annotationAdd
    BLAST
    Transmembranei330 – 35324Helical; Name=VUniRule annotationAdd
    BLAST
    Transmembranei369 – 39527Helical; Name=VIUniRule annotationAdd
    BLAST
    Transmembranei417 – 43923Helical; Name=VIIUniRule annotationAdd
    BLAST
    Transmembranei517 – 53519Helical; Name=VIIIUniRule annotationAdd
    BLAST
    Transmembranei575 – 59622Helical; Name=IXUniRule annotationAdd
    BLAST
    Transmembranei643 – 66523Helical; Name=XUniRule annotationAdd
    BLAST
    Transmembranei707 – 72721Helical; Name=XIUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PsaA/PsaB family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.1130.10. 1 hit.
    HAMAPiMF_00482. PSI_PsaB.
    InterProiIPR001280. PSI_PsaA/B.
    IPR020586. PSI_PsaA/B_CS.
    IPR006244. PSI_PsaB.
    [Graphical view]
    PfamiPF00223. PsaA_PsaB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002905. PSI_A. 1 hit.
    PRINTSiPR00257. PHOTSYSPSAAB.
    SUPFAMiSSF81558. SSF81558. 1 hit.
    TIGRFAMsiTIGR01336. psaB. 1 hit.
    PROSITEiPS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05311-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALRIPRFSQ GIAQDPTTRR IWFGIATAHD FESHDDITEG RLYQNIFASH    50
    FGQLAIIFLW TSGNLFHVAW QGNFEAWVQD PFHVRPIAHA IWDPHFGQPA 100
    VEAFTRGGAL GPVNNAYSGV YQWWYTIGLR TNEDLYTGAI FLLFLSFISL 150
    LAGWLHLQPK WKPSVSWFKN AESRLNHHLS GLFGVSSLAW AGHLVHVAIP 200
    GSRGEYVRWN NFLDVLPYPQ GLGPLLTGQW NLYAQNPSSS NHLFGTTQGA 250
    GTAILTILGG FHPQTQSLWL TDVAHHHLAI AFLFLIGGLM YRTNFGIGHS 300
    IKYILEAHIP PGGRLGRGHK GLYDTINNSI HFQLGLALAS LGVITSLVAQ 350
    HMYSLPAYAF IAQDFTTQAA LYTHHQYIAG FIMTGAFAHG PIFFIRDYNP 400
    EQNADNVLAR MLEHKEAIIS HLSWASLFLG FHTLGLYVHN DVMLAFGTPE 450
    KQILIEPIFA QWIQSAHGKT TYGFDIPLSS TNGPALNAGR NIWLPGWLNA 500
    INENSNSLFL TIGPGDFLVH HAIALGLHTT TLILVKGALD ARGSKLMPDK 550
    KDFGYSFPCD GPGRGGTCDI SAWDDFYLAV FWMLNTIGWV TFYWHWKHIT 600
    LWRGNVSQFN ESSTYLMGWL RDYLWLNSSQ LINGITPLVC NSLSVWAWMF 650
    LFGHLVWATG FMFLISWRGY WQELIETLAW AHERTPLANL IRWRDKPVAL 700
    SIVQARLVGL VHFSVGYIFT YAAFLIASTS GKFG 734
    Length:734
    Mass (Da):82,412
    Last modified:November 1, 1988 - v1
    Checksum:i2ACF1F77D46FA8E7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05423 Genomic DNA. Translation: CAA29004.1.
    PIRiS00704.
    RefSeqiYP_003587537.1. NC_014057.1.

    Genome annotation databases

    GeneIDi9073070.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05423 Genomic DNA. Translation: CAA29004.1 .
    PIRi S00704.
    RefSeqi YP_003587537.1. NC_014057.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YO9 model - B 1-734 [» ]
    2O01 X-ray 3.40 B 2-733 [» ]
    2WSC X-ray 3.30 B 1-734 [» ]
    2WSE X-ray 3.49 B 1-734 [» ]
    2WSF X-ray 3.48 B 1-734 [» ]
    3LW5 X-ray 3.30 B 2-734 [» ]
    ProteinModelPortali P05311.
    SMRi P05311. Positions 2-733.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60282N.

    Proteomic databases

    PRIDEi P05311.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 9073070.

    Miscellaneous databases

    EvolutionaryTracei P05311.

    Family and domain databases

    Gene3Di 1.20.1130.10. 1 hit.
    HAMAPi MF_00482. PSI_PsaB.
    InterProi IPR001280. PSI_PsaA/B.
    IPR020586. PSI_PsaA/B_CS.
    IPR006244. PSI_PsaB.
    [Graphical view ]
    Pfami PF00223. PsaA_PsaB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002905. PSI_A. 1 hit.
    PRINTSi PR00257. PHOTSYSPSAAB.
    SUPFAMi SSF81558. SSF81558. 1 hit.
    TIGRFAMsi TIGR01336. psaB. 1 hit.
    PROSITEi PS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of two genes in pea chloroplast DNA coding for 84 and 82 kD polypeptides of the photosystem I complex."
      Lehmbeck J., Rasmussen O.F., Bookjans G.B., Jepsen B.R., Stummann B.M., Henningsen K.W.
      Plant Mol. Biol. 7:3-10(1986)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPSAB_PEA
    AccessioniPrimary (citable) accession number: P05311
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3