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P05310

- PSAA_PEA

UniProt

P05310 - PSAA_PEA

Protein

Photosystem I P700 chlorophyll a apoprotein A1

Gene

psaA

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (18 Oct 2001)
      Previous versions | rss
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    Functioni

    PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin.

    Catalytic activityi

    Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin.UniRule annotation

    Cofactori

    P700 is a chlorophyll a/chlorophyll a' dimer, A0 is one or more chlorophyll a, A1 is one or both phylloquinones and FX is a shared 4Fe-4S iron-sulfur center.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi581 – 5811Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
    Metal bindingi590 – 5901Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
    Metal bindingi683 – 6831Magnesium (chlorophyll-a' A1 axial ligand; P700 special pair)UniRule annotation
    Metal bindingi691 – 6911Magnesium (chlorophyll-a A3 axial ligand)UniRule annotation
    Binding sitei699 – 6991Chlorophyll-a A3UniRule annotation
    Binding sitei700 – 7001Phylloquinone AUniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. chlorophyll binding Source: UniProtKB-KW
    3. electron carrier activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. oxidoreductase activity Source: UniProtKB-KW

    GO - Biological processi

    1. photosynthesis Source: UniProtKB-HAMAP
    2. protein-chromophore linkage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    4Fe-4S, Chlorophyll, Chromophore, Iron, Iron-sulfur, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Photosystem I P700 chlorophyll a apoprotein A1UniRule annotation (EC:1.97.1.12UniRule annotation)
    Alternative name(s):
    PSI-AUniRule annotation
    PsaAUniRule annotation
    Gene namesi
    Name:psaAUniRule annotation
    Synonyms:psaA1
    Encoded oniPlastid; Chloroplast
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    Plastidchloroplast thylakoid membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

    GO - Cellular componenti

    1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. photosystem I Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Membrane, Photosystem I, Plastid, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 758758Photosystem I P700 chlorophyll a apoprotein A1PRO_0000088568Add
    BLAST

    Interactioni

    Subunit structurei

    The PsaA/B heterodimer binds the P700 chlorophyll special pair and subsequent electron acceptors. PSI consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The eukaryotic PSI reaction center is composed of at least 11 subunits.UniRule annotation

    Protein-protein interaction databases

    DIPiDIP-60281N.

    Structurei

    Secondary structure

    1
    758
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi26 – 283
    Beta strandi35 – 373
    Beta strandi40 – 434
    Helixi52 – 543
    Beta strandi60 – 623
    Beta strandi64 – 674
    Beta strandi69 – 713
    Helixi73 – 819
    Turni82 – 854
    Helixi86 – 894
    Turni90 – 934
    Helixi94 – 974
    Beta strandi98 – 1036
    Helixi106 – 1116
    Turni113 – 1153
    Beta strandi119 – 1213
    Turni127 – 1326
    Beta strandi137 – 1393
    Beta strandi147 – 1493
    Beta strandi150 – 1523
    Helixi153 – 1553
    Helixi161 – 17313
    Turni174 – 1763
    Helixi183 – 1875
    Helixi193 – 1975
    Turni200 – 2023
    Helixi203 – 2064
    Helixi207 – 2115
    Turni213 – 2153
    Helixi216 – 2194
    Beta strandi222 – 2254
    Helixi228 – 2303
    Turni231 – 2355
    Turni250 – 2534
    Turni255 – 2584
    Beta strandi267 – 2715
    Helixi274 – 2785
    Turni279 – 2813
    Beta strandi289 – 2913
    Helixi296 – 31116
    Beta strandi319 – 3224
    Helixi328 – 3303
    Turni331 – 3333
    Beta strandi337 – 3393
    Helixi341 – 3433
    Turni344 – 3474
    Beta strandi350 – 3523
    Helixi355 – 36713
    Turni368 – 3714
    Helixi372 – 3754
    Turni376 – 3783
    Beta strandi382 – 3843
    Helixi389 – 3924
    Helixi394 – 3985
    Helixi402 – 4054
    Turni406 – 4083
    Helixi409 – 41911
    Beta strandi420 – 4256
    Helixi431 – 4377
    Turni440 – 4467
    Helixi447 – 4559
    Turni456 – 4583
    Helixi459 – 46810
    Turni469 – 4713
    Turni479 – 4813
    Beta strandi482 – 4843
    Turni490 – 4923
    Helixi493 – 4953
    Turni496 – 4983
    Beta strandi502 – 5043
    Beta strandi508 – 5114
    Beta strandi515 – 5184
    Beta strandi524 – 5263
    Turni536 – 5405
    Helixi541 – 56121
    Beta strandi562 – 5654
    Beta strandi573 – 5753
    Turni586 – 5883
    Turni595 – 5995
    Helixi600 – 62223
    Helixi630 – 6323
    Beta strandi637 – 6404
    Helixi642 – 6443
    Beta strandi646 – 6494
    Helixi651 – 6544
    Turni655 – 66612
    Beta strandi670 – 6723
    Turni674 – 68512
    Helixi686 – 6883
    Helixi689 – 6935
    Helixi698 – 71417
    Beta strandi719 – 7213
    Helixi728 – 7325
    Turni733 – 7353
    Helixi736 – 75015
    Beta strandi753 – 7564

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YO9model-A1-758[»]
    2O01X-ray3.40A5-758[»]
    2WSCX-ray3.30A1-758[»]
    2WSEX-ray3.49A1-758[»]
    2WSFX-ray3.48A1-758[»]
    3LW5X-ray3.30A21-758[»]
    ProteinModelPortaliP05310.
    SMRiP05310. Positions 31-758.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05310.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei78 – 10124Helical; Name=IUniRule annotationAdd
    BLAST
    Transmembranei164 – 18724Helical; Name=IIUniRule annotationAdd
    BLAST
    Transmembranei203 – 22725Helical; Name=IIIUniRule annotationAdd
    BLAST
    Transmembranei299 – 31719Helical; Name=IVUniRule annotationAdd
    BLAST
    Transmembranei354 – 37724Helical; Name=VUniRule annotationAdd
    BLAST
    Transmembranei393 – 41927Helical; Name=VIUniRule annotationAdd
    BLAST
    Transmembranei441 – 46323Helical; Name=VIIUniRule annotationAdd
    BLAST
    Transmembranei539 – 55719Helical; Name=VIIIUniRule annotationAdd
    BLAST
    Transmembranei597 – 61822Helical; Name=IXUniRule annotationAdd
    BLAST
    Transmembranei672 – 69423Helical; Name=XUniRule annotationAdd
    BLAST
    Transmembranei732 – 75322Helical; Name=XIUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PsaA/PsaB family.UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.1130.10. 1 hit.
    HAMAPiMF_00458. PSI_PsaA.
    InterProiIPR006243. PSI_PsaA.
    IPR001280. PSI_PsaA/B.
    IPR020586. PSI_PsaA/B_CS.
    [Graphical view]
    PfamiPF00223. PsaA_PsaB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002905. PSI_A. 1 hit.
    PRINTSiPR00257. PHOTSYSPSAAB.
    SUPFAMiSSF81558. SSF81558. 1 hit.
    TIGRFAMsiTIGR01335. psaA. 1 hit.
    PROSITEiPS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05310-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIIRSPEPKV QILADPEVKI LVDRDPIKTS FEQWAKPGHF SRTIAKGPDT    50
    TTWIWNLHAD AHDFDSHTSD LEEISRKVFS AHFGQLSIIF LWLSGMYFHG 100
    ARFSNYEAWL NDPTHIGPSA QVVWPIVGQE ILNGDVGGGF RGIQITSGFF 150
    QIWRASGITS ELQLYCTAIG ALVFAGLMLF AGWFHYHKAA PKLAWFQDVE 200
    SMLNHHLAGL LGLGSLSWAR HQVHVSLPIN QFLNAGVDPK EIPLPHEFIL 250
    NRDLLAQLYP SFAEGATPFF TLNWSKYADF LTFRGGLDPL TGGLWLTDIA 300
    HHHLAIAILF LIAGHMYRTN WGIGHGIKDI LEAHKGPFTG QGHKGLYEIL 350
    TTSWHAQLSI NLAMLGSLTI VVAQHMYSMP PYPYLATDYA TQLSLFTHHM 400
    WIGGFLIVGA AAHAAIFMVR DYDPTTRYND LLDRVLRHRD AIISHLNWVC 450
    IFLGFHSFGL YIHNDTMSAL GRPQDMFSDT AIQLQPVFAQ WIQNTHALAP 500
    GTTAPGATAS TSLTWGGGDL VAVGNKVALL PIPLGTADFL VHHIHAFTIH 550
    VTVLILLKGV LFARSSRLIP DKANLGFRFP CDGPGRGGTC QVSAWDHVFL 600
    GLFWMYNSIS VVIFHFSWKM QSDVWGTIND QGVVTHITAG NFAQSSITIN 650
    GWLRDFLWAQ ASQVIQSYGS SLSAYGLFFL GAHFVWAFSL MFLFSGRGYW 700
    QELIESIVWA HNKLKVAPAT QPRALSIVQG RAVGVTHYLL GGIATTWAFF 750
    LARIIAVG 758
    Length:758
    Mass (Da):84,199
    Last modified:October 18, 2001 - v2
    Checksum:i4B6CA42A585294A2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti117 – 1171G → R in CAA29003. 1 PublicationCurated
    Sequence conflicti176 – 1761G → A in CAA29003. 1 PublicationCurated
    Sequence conflicti194 – 1941A → V in CAA29003. 1 PublicationCurated
    Sequence conflicti220 – 2201R → G in CAA29003. 1 PublicationCurated
    Sequence conflicti239 – 25315Missing1 PublicationCuratedAdd
    BLAST
    Sequence conflicti345 – 3451G → GPFTGQGHKGPFTGQGHKG1 PublicationCurated
    Sequence conflicti371 – 3788VVAQHMYS → IAAHHMIA in CAA29003. 1 PublicationCurated
    Sequence conflicti382 – 3821Y → I in CAA29003. 1 PublicationCurated
    Sequence conflicti390 – 3901A → G in CAA29003. 1 PublicationCurated
    Sequence conflicti509 – 5091A → T in CAA29003. 1 PublicationCurated
    Sequence conflicti514 – 5141T → I in CAA29003. 1 PublicationCurated
    Sequence conflicti522 – 5221A → S in CAA29003. 1 PublicationCurated
    Sequence conflicti525 – 5251N → G in CAA29003. 1 PublicationCurated
    Sequence conflicti596 – 5961D → V in CAA29003. 1 PublicationCurated
    Sequence conflicti608 – 6081S → A in CAA29003. 1 PublicationCurated
    Sequence conflicti627 – 6271T → S in CAA29003. 1 PublicationCurated
    Sequence conflicti639 – 6413AGN → GGR in CAA29003. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05423 Genomic DNA. Translation: CAA29003.1.
    AF223227 Genomic DNA. Translation: AAF65219.1.
    PIRiS00703.
    RefSeqiYP_003587538.1. NC_014057.1.

    Genome annotation databases

    GeneIDi9073071.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05423 Genomic DNA. Translation: CAA29003.1 .
    AF223227 Genomic DNA. Translation: AAF65219.1 .
    PIRi S00703.
    RefSeqi YP_003587538.1. NC_014057.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YO9 model - A 1-758 [» ]
    2O01 X-ray 3.40 A 5-758 [» ]
    2WSC X-ray 3.30 A 1-758 [» ]
    2WSE X-ray 3.49 A 1-758 [» ]
    2WSF X-ray 3.48 A 1-758 [» ]
    3LW5 X-ray 3.30 A 21-758 [» ]
    ProteinModelPortali P05310.
    SMRi P05310. Positions 31-758.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60281N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 9073071.

    Miscellaneous databases

    EvolutionaryTracei P05310.

    Family and domain databases

    Gene3Di 1.20.1130.10. 1 hit.
    HAMAPi MF_00458. PSI_PsaA.
    InterProi IPR006243. PSI_PsaA.
    IPR001280. PSI_PsaA/B.
    IPR020586. PSI_PsaA/B_CS.
    [Graphical view ]
    Pfami PF00223. PsaA_PsaB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002905. PSI_A. 1 hit.
    PRINTSi PR00257. PHOTSYSPSAAB.
    SUPFAMi SSF81558. SSF81558. 1 hit.
    TIGRFAMsi TIGR01335. psaA. 1 hit.
    PROSITEi PS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of two genes in pea chloroplast DNA coding for 84 and 82 kD polypeptides of the photosystem I complex."
      Lehmbeck J., Rasmussen O.F., Bookjans G.B., Jepsen B.R., Stummann B.M., Henningsen K.W.
      Plant Mol. Biol. 7:3-10(1986)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Error, bias, and long-branch attraction in data for two chloroplast photosystem genes in seed plants."
      Sanderson M.J., Wojciechowski M.F., Hu J.-M., Sher Khan T., Brady S.G.
      Mol. Biol. Evol. 17:782-797(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-737.
      Strain: cv. Wojciechowski 398.

    Entry informationi

    Entry nameiPSAA_PEA
    AccessioniPrimary (citable) accession number: P05310
    Secondary accession number(s): Q9MUB9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: October 18, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3