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P05310 (PSAA_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Photosystem I P700 chlorophyll a apoprotein A1

EC=1.97.1.12
Alternative name(s):
PSI-A
PsaA
Gene names
Name:psaA
Synonyms:psaA1
Encoded onPlastid; Chloroplast
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length758 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin. HAMAP-Rule MF_00458

Catalytic activity

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin. HAMAP-Rule MF_00458

Cofactor

P700 is a chlorophyll a/chlorophyll a' dimer, A0 is one or more chlorophyll a, A1 is one or both phylloquinones and FX is a shared 4Fe-4S iron-sulfur center By similarity.

Subunit structure

The PsaA/B heterodimer binds the P700 chlorophyll special pair and subsequent electron acceptors. PSI consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The eukaryotic PSI reaction center is composed of at least 11 subunits By similarity.

Subcellular location

Plastidchloroplast thylakoid membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00458.

Sequence similarities

Belongs to the PsaA/PsaB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 758758Photosystem I P700 chlorophyll a apoprotein A1 HAMAP-Rule MF_00458
PRO_0000088568

Regions

Transmembrane78 – 10124Helical; Name=I; Potential
Transmembrane164 – 18724Helical; Name=II; Potential
Transmembrane203 – 22725Helical; Name=III; Potential
Transmembrane299 – 31719Helical; Name=IV; Potential
Transmembrane354 – 37724Helical; Name=V; Potential
Transmembrane393 – 41927Helical; Name=VI; Potential
Transmembrane441 – 46323Helical; Name=VII; Potential
Transmembrane539 – 55719Helical; Name=VIII; Potential
Transmembrane597 – 61822Helical; Name=IX; Potential
Transmembrane672 – 69423Helical; Name=X; Potential
Transmembrane732 – 75322Helical; Name=XI; Potential

Sites

Metal binding5811Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
Metal binding5901Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
Metal binding6831Magnesium (chlorophyll-a' A1 axial ligand; P700 special pair) By similarity
Metal binding6911Magnesium (chlorophyll-a A3 axial ligand) By similarity
Binding site6991Chlorophyll-a A3 By similarity
Binding site7001Phylloquinone A By similarity

Experimental info

Sequence conflict1171G → R in CAA29003. Ref.1
Sequence conflict1761G → A in CAA29003. Ref.1
Sequence conflict1941A → V in CAA29003. Ref.1
Sequence conflict2201R → G in CAA29003. Ref.1
Sequence conflict239 – 25315Missing Ref.1
Sequence conflict3451G → GPFTGQGHKGPFTGQGHKG Ref.1
Sequence conflict371 – 3788VVAQHMYS → IAAHHMIA in CAA29003. Ref.1
Sequence conflict3821Y → I in CAA29003. Ref.1
Sequence conflict3901A → G in CAA29003. Ref.1
Sequence conflict5091A → T in CAA29003. Ref.1
Sequence conflict5141T → I in CAA29003. Ref.1
Sequence conflict5221A → S in CAA29003. Ref.1
Sequence conflict5251N → G in CAA29003. Ref.1
Sequence conflict5961D → V in CAA29003. Ref.1
Sequence conflict6081S → A in CAA29003. Ref.1
Sequence conflict6271T → S in CAA29003. Ref.1
Sequence conflict639 – 6413AGN → GGR in CAA29003. Ref.1

Secondary structure

............................................................................................................................................................. 758
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P05310 [UniParc].

Last modified October 18, 2001. Version 2.
Checksum: 4B6CA42A585294A2

FASTA75884,199
        10         20         30         40         50         60 
MIIRSPEPKV QILADPEVKI LVDRDPIKTS FEQWAKPGHF SRTIAKGPDT TTWIWNLHAD 

        70         80         90        100        110        120 
AHDFDSHTSD LEEISRKVFS AHFGQLSIIF LWLSGMYFHG ARFSNYEAWL NDPTHIGPSA 

       130        140        150        160        170        180 
QVVWPIVGQE ILNGDVGGGF RGIQITSGFF QIWRASGITS ELQLYCTAIG ALVFAGLMLF 

       190        200        210        220        230        240 
AGWFHYHKAA PKLAWFQDVE SMLNHHLAGL LGLGSLSWAR HQVHVSLPIN QFLNAGVDPK 

       250        260        270        280        290        300 
EIPLPHEFIL NRDLLAQLYP SFAEGATPFF TLNWSKYADF LTFRGGLDPL TGGLWLTDIA 

       310        320        330        340        350        360 
HHHLAIAILF LIAGHMYRTN WGIGHGIKDI LEAHKGPFTG QGHKGLYEIL TTSWHAQLSI 

       370        380        390        400        410        420 
NLAMLGSLTI VVAQHMYSMP PYPYLATDYA TQLSLFTHHM WIGGFLIVGA AAHAAIFMVR 

       430        440        450        460        470        480 
DYDPTTRYND LLDRVLRHRD AIISHLNWVC IFLGFHSFGL YIHNDTMSAL GRPQDMFSDT 

       490        500        510        520        530        540 
AIQLQPVFAQ WIQNTHALAP GTTAPGATAS TSLTWGGGDL VAVGNKVALL PIPLGTADFL 

       550        560        570        580        590        600 
VHHIHAFTIH VTVLILLKGV LFARSSRLIP DKANLGFRFP CDGPGRGGTC QVSAWDHVFL 

       610        620        630        640        650        660 
GLFWMYNSIS VVIFHFSWKM QSDVWGTIND QGVVTHITAG NFAQSSITIN GWLRDFLWAQ 

       670        680        690        700        710        720 
ASQVIQSYGS SLSAYGLFFL GAHFVWAFSL MFLFSGRGYW QELIESIVWA HNKLKVAPAT 

       730        740        750 
QPRALSIVQG RAVGVTHYLL GGIATTWAFF LARIIAVG 

« Hide

References

[1]"Sequence of two genes in pea chloroplast DNA coding for 84 and 82 kD polypeptides of the photosystem I complex."
Lehmbeck J., Rasmussen O.F., Bookjans G.B., Jepsen B.R., Stummann B.M., Henningsen K.W.
Plant Mol. Biol. 7:3-10(1986) [AGRICOLA] [Europe PMC]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Error, bias, and long-branch attraction in data for two chloroplast photosystem genes in seed plants."
Sanderson M.J., Wojciechowski M.F., Hu J.-M., Sher Khan T., Brady S.G.
Mol. Biol. Evol. 17:782-797(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-737.
Strain: cv. Wojciechowski 398.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05423 Genomic DNA. Translation: CAA29003.1.
AF223227 Genomic DNA. Translation: AAF65219.1.
PIRS00703.
RefSeqYP_003587538.1. NC_014057.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YO9model-A1-758[»]
2O01X-ray3.40A5-758[»]
2WSCX-ray3.30A1-758[»]
2WSEX-ray3.49A1-758[»]
2WSFX-ray3.48A1-758[»]
3LW5X-ray3.30A21-758[»]
ProteinModelPortalP05310.
SMRP05310. Positions 31-758.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60281N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID9073071.

Family and domain databases

Gene3D1.20.1130.10. 1 hit.
HAMAPMF_00458. PSI_PsaA.
InterProIPR006243. PSI_PsaA.
IPR001280. PSI_PsaA/B.
IPR020586. PSI_PsaA/B_CS.
[Graphical view]
PfamPF00223. PsaA_PsaB. 1 hit.
[Graphical view]
PIRSFPIRSF002905. PSI_A. 1 hit.
PRINTSPR00257. PHOTSYSPSAAB.
SUPFAMSSF81558. SSF81558. 1 hit.
TIGRFAMsTIGR01335. psaA. 1 hit.
PROSITEPS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP05310.

Entry information

Entry namePSAA_PEA
AccessionPrimary (citable) accession number: P05310
Secondary accession number(s): Q9MUB9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: October 18, 2001
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references