P05305 (EDN1_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 140.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Endothelin-1 Alternative name(s): Preproendothelin-1 Short name=PPET1 Cleaved into the following 2 chains:
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| Gene names |
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| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 212 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Endothelins are endothelium-derived vasoconstrictor peptides. |
| Subcellular location | |
| Tissue specificity | Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Ref.11 |
| Sequence similarities | Belongs to the endothelin/sarafotoxin family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||
Molecule processing | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 17 | 17 | |||||||||||
| Propeptide | 18 – 50 | 33 | PRO_0000008058 | ||||||||||
| Peptide | 53 – 90 | 38 | Big endothelin-1 | PRO_0000008059 | |||||||||
| Peptide | 53 – 73 | 21 | Endothelin-1 | PRO_0000008060 | |||||||||
| Propeptide | 74 – 212 | 139 | PRO_0000008061 | ||||||||||
Regions | |||||||||||||
| Region | 109 – 123 | 15 | Endothelin-like | ||||||||||
Sites | |||||||||||||
| Site | 73 – 74 | 2 | Cleavage; by KEL | ||||||||||
Amino acid modifications | |||||||||||||
| Disulfide bond | 53 ↔ 67 | Ref.13 | |||||||||||
| Disulfide bond | 55 ↔ 63 | Ref.13 | |||||||||||
| Disulfide bond | 109 ↔ 123 | By similarity | |||||||||||
| Disulfide bond | 111 ↔ 119 | By similarity | |||||||||||
Natural variations | |||||||||||||
| Natural variant | 186 | 1 | V → I. Corresponds to variant rs6413478 [ dbSNP | Ensembl ]. | VAR_048933 | |||||||||
| Natural variant | 198 | 1 | K → N. Ref.5 Ref.7 Ref.19 Ref.20 Corresponds to variant rs5370 [ dbSNP | Ensembl ]. | VAR_014188 | |||||||||
Secondary structure | |||||||||||||
Helix Strand Turn | |||||||||||||
| Beta strand | 53 – 56 | 4 | |||||||||||
| Helix | 61 – 67 | 7 | |||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and sequence analysis of cDNA encoding the precursor of a human endothelium-derived vasoconstrictor peptide, endothelin: identity of human and porcine endothelin." Itoh Y., Yanagisawa M., Ohkubo S., Kimura C., Kosaka T., Inoue A., Ishida N., Mitsui Y., Onda H., Fujino M., Masaki T. FEBS Lett. 231:440-444(1988) [PubMed: 3282927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta. |
| [2] | "The human preproendothelin-1 gene. Complete nucleotide sequence and regulation of expression." Inoue A., Yanagisawa M., Takuwa Y., Mitsui Y., Kobayashi M., Masaki T. J. Biol. Chem. 264:14954-14959(1989) [PubMed: 2670930] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Structural organization and chromosomal assignment of the gene encoding endothelin." Bloch K.D., Friedrich S.P., Lee M.E., Eddy R.L., Shows T.B., Quertermous T. J. Biol. Chem. 264:10851-10857(1989) [PubMed: 2659594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "Two preproendothelin 1 mRNAs transcribed by alternative promoters." Benatti L., Bonecchi L., Cozzi L., Sarmientos P. J. Clin. Invest. 91:1149-1156(1993) [PubMed: 8450044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [5] | NIEHS SNPs program Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASN-198. |
| [6] | "The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S.Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [7] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASN-198. Tissue: Pancreas. |
| [8] | "The human endothelin family: three structurally and pharmacologically distinct isopeptides predicted by three separate genes." Inoue A., Yanagisawa M., Kimura S., Kasuya Y., Miyauchi T., Goto K., Masaki T. Proc. Natl. Acad. Sci. U.S.A. 86:2863-2867(1989) [PubMed: 2649896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-67. |
| [9] | "Proteolytic processing of big endothelin-3 by the kell blood group protein." Lee S., Lin M., Mele A., Cao Y., Farmar J., Russo D., Redman C. Blood 94:1440-1450(1999) [PubMed: 10438732] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CLEAVAGE BY KELL, MASS SPECTROMETRY. |
| [10] | "The signal peptide of human preproendothelin-1." Fabbrini M.S., Valsasina B., Nitti G., Benatti L., Vitale A. FEBS Lett. 286:91-94(1991) [PubMed: 1864385] [Abstract] Cited for: PROTEOLYTIC PROCESSING. |
| [11] | "Endothelin-1 and ETA receptor expression in vascular smooth muscle cells from human placenta: a new ETA receptor messenger ribonucleic acid is generated by alternative splicing of exon 3." Bourgeois C., Robert B., Rebourcet R., Mondon F., Mignot T.-M., Duc-Goiran P., Ferre F. J. Clin. Endocrinol. Metab. 82:3116-3123(1997) [PubMed: 9284755] [Abstract] Cited for: TISSUE SPECIFICITY. |
| [12] | "Crystallization and preliminary X-ray analysis of human endothelin." Wolff M., Day J., Greenwood A., Larson S., McPherson A. Acta Crystallogr. B 48:239-240(1992) [PubMed: 1515112] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF ET-1. |
| [13] | "The crystal structure of human endothelin." Janes R.W., Peapus D.H., Wallace B.A. Nat. Struct. Biol. 1:311-319(1994) [PubMed: 7664037] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.18 ANGSTROMS) OF ET-1, DISULFIDE BONDS. |
| [14] | "The conformation of endothelin-1 in aqueous solution: NMR-derived constraints combined with distance geometry and molecular dynamics calculations." Reily M.D., Dunbar J.B. Jr. Biochem. Biophys. Res. Commun. 178:570-577(1991) [PubMed: 1859417] [Abstract] Cited for: STRUCTURE BY NMR OF ET-1. |
| [15] | "Conformational isomerism of endothelin in acidic aqueous media: a quantitative NOESY analysis." Andersen N.H., Chen C., Marschner T.M., Krystek S.R. Jr., Bassolino D.A. Biochemistry 31:1280-1295(1992) [PubMed: 1736987] [Abstract] Cited for: STRUCTURE BY NMR OF ET-1. |
| [16] | "Solution conformation of human big endothelin-1." Donlan M.L., Brown F.K., Jeffs P.W. J. Biomol. NMR 2:407-420(1992) [PubMed: 1422154] [Abstract] Cited for: STRUCTURE BY NMR OF 53-90. |
| [17] | "A comparison of X-ray and NMR structures for human endothelin-1." Wallace B.A., Janes R.W., Bassolino D.A., Krystek S.R. Jr. Protein Sci. 4:75-83(1995) [PubMed: 7773179] [Abstract] Cited for: COMPARISON OF NMR STRUCTURE WITH X-RAY STRUCTURE. |
| [18] | "Solution structure of a novel ETB receptor selective agonist ET1-21 [Cys(Acm)1,15, Aib3,11, Leu7] by nuclear magnetic resonance spectroscopy and molecular modelling." Hewage C.M., Jiang L., Parkinson J.A., Ramage R., Sadler I.H. J. Pept. Res. 53:223-233(1999) [PubMed: 10231710] [Abstract] Cited for: STRUCTURE BY NMR OF ET1 AGONIST. |
| [19] | "The Lys198Asn polymorphism in the endothelin-1 gene is associated with blood pressure in overweight people." Tiret L., Poirier O., Hallet V., McDonagh T.A., Morrison C., McMurray J.J., Dargie H.J., Arveiler D., Ruidavets J.B., Luc G., Evans A., Cambien F. Hypertension 33:1169-1174(1999) [PubMed: 10334806] [Abstract] Cited for: VARIANT ASN-198. |
| [20] | "Patterns of single-nucleotide polymorphisms in candidate genes for blood-pressure homeostasis." Halushka M.K., Fan J.-B., Bentley K., Hsie L., Shen N., Weder A., Cooper R., Lipshutz R., Chakravarti A. Nat. Genet. 22:239-247(1999) [PubMed: 10391210] [Abstract] Cited for: VARIANT ASN-198. |
| + | Additional computationally mapped references. |
Web resources
| NIEHS-SNPs |
| R&D Systems' cytokine source book: Endothelin-1 |
| Wikipedia Endothelin entry |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | Y00749 mRNA. Translation: CAA68718.1. M25380 M25379 Genomic DNA. Translation: AAA52407.1.J05008 Genomic DNA. Translation: AAA52339.1. S56805 mRNA. Translation: AAB25760.1. AY434104 Genomic DNA. Translation: AAQ96600.1. Z98050 Genomic DNA. Translation: CAB10846.1. BC009720 mRNA. Translation: AAH09720.1. M25549 Genomic DNA. Translation: AAA52338.1. | ||||||||||||||||||||||||||||||
| IPI | IPI00008327. | ||||||||||||||||||||||||||||||
| PIR | ANHU1. A36517. | ||||||||||||||||||||||||||||||
| RefSeq | NP_001161791.1. NM_001168319.1. NP_001946.3. NM_001955.4. | ||||||||||||||||||||||||||||||
| UniGene | Hs.511899. Hs.713645. | ||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | P05305. | ||||||||||||||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||
| IntAct | P05305. 3 interactions. | ||||||||||||||||||||||||||||||
| MINT | MINT-1387120. | ||||||||||||||||||||||||||||||
| STRING | P05305. | ||||||||||||||||||||||||||||||
Polymorphism databases | |||||||||||||||||||||||||||||||
| DMDM | 119610. | ||||||||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||||||||
| PRIDE | P05305. | ||||||||||||||||||||||||||||||
Protocols and materials databases | |||||||||||||||||||||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||
| Ensembl | ENST00000379375; ENSP00000368683; ENSG00000078401. | ||||||||||||||||||||||||||||||
| GeneID | 1906. | ||||||||||||||||||||||||||||||
| KEGG | hsa:1906. | ||||||||||||||||||||||||||||||
| UCSC | uc003nae.2. human. | ||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||
| CTD | 1906. | ||||||||||||||||||||||||||||||
| GeneCards | GC06P012290. | ||||||||||||||||||||||||||||||
| H-InvDB | HIX0005582. HIX0033211. | ||||||||||||||||||||||||||||||
| HGNC | HGNC:3176. EDN1. | ||||||||||||||||||||||||||||||
| HPA | CAB032500. HPA031976. | ||||||||||||||||||||||||||||||
| MIM | 131240. gene. | ||||||||||||||||||||||||||||||
| neXtProt | NX_P05305. | ||||||||||||||||||||||||||||||
| PharmGKB | PA27614. | ||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||
| eggNOG | prNOG19729. | ||||||||||||||||||||||||||||||
| HOGENOM | HBG279519. | ||||||||||||||||||||||||||||||
| HOVERGEN | HBG051441. | ||||||||||||||||||||||||||||||
| InParanoid | P05305. | ||||||||||||||||||||||||||||||
| OMA | QKDKKCW. | ||||||||||||||||||||||||||||||
| OrthoDB | EOG4QZ7N8. | ||||||||||||||||||||||||||||||
| PhylomeDB | P05305. | ||||||||||||||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||||||||||||||
| Pathway_Interaction_DB | endothelinpathway. Endothelins. hif1_tfpathway. HIF-1-alpha transcription factor network. | ||||||||||||||||||||||||||||||
| Reactome | REACT_111102. Signal Transduction. | ||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||
| ArrayExpress | P05305. | ||||||||||||||||||||||||||||||
| Bgee | P05305. | ||||||||||||||||||||||||||||||
| CleanEx | HS_EDN1. | ||||||||||||||||||||||||||||||
| Genevestigator | P05305. | ||||||||||||||||||||||||||||||
| GermOnline | ENSG00000078401. Homo sapiens. | ||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||
| InterPro | IPR020475. Bibrotoxin/Sarafotoxin-D. IPR019764. Endothelin_toxin_CS. IPR001928. Endothln-like_toxin. [Graphical view] | ||||||||||||||||||||||||||||||
| KO | K05227. | ||||||||||||||||||||||||||||||
| Pfam | PF00322. Endothelin. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| PRINTS | PR00365. ENDOTHELIN. | ||||||||||||||||||||||||||||||
| SMART | SM00272. END. 2 hits. [Graphical view] | ||||||||||||||||||||||||||||||
| PROSITE | PS00270. ENDOTHELIN. 2 hits. [Graphical view] | ||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||
Other | |||||||||||||||||||||||||||||||
| NextBio | 7757. | ||||||||||||||||||||||||||||||
| PMAP-CutDB | P05305. | ||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||
Entry information
| Entry name | EDN1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P05305 Secondary accession number(s): Q96DA1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 6 Human chromosome 6: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with