P05302 (MTD1_DESNO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase DdeI
Short name=M.DdeI
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase DdeI

Gene names

Name:	ddeIM
Synonyms:	ddeM

Organism

Desulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))

Taxonomic identifier

52561 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfovibrionales › Desulfomicrobiaceae › Desulfomicrobium

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Modification methylase DdeI		PRO_0000087873
Sites
	Active site	76	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05302 [UniParc]. Last modified November 1, 1988. Version 1. Checksum: 34DF5968E0414EED	FASTA	415	47,081
	10 20 30 40 50 60 MNIIDLFAGC GGFSHGFKMA GYNSILAIEK DLWASQTYSF NNPNVSVITE DITTLDPGDL 70 80 90 100 110 120 KISVSDVDGI IGGPPCQGFS LSGNRDQKDP RNSLFVDFVR FVKFFSPKFF VMENVLGILS 130 140 150 160 170 180 MKTKSRQYVK DIIAEEFSNV GYKVCVIILN ACDYGVPQSR QRVFFIGLKS DRPLNQQILT 190 200 210 220 230 240 PPSKVIESEY TSLEEAISDL PVIEAGEGGE VQDYPVAPRN KYQENMRKGS TCVYNHVAMR 250 260 270 280 290 300 HTQRLVDRFA AIKFGQSVKH VSEEHSQRKR GDANSISGKV FSQNNMRPYP YKPCPTVAAS 310 320 330 340 350 360 FQSNFIHPFY NRNFTAREGA RIQSFPDTYI FQGKRTTMSW EKHLSQYQQI GNAVPPLLAQ 370 380 390 400 410 ALAERISWYF ENINLINDSN VSIKRMVQRS FMSQLNLENN VNVRQDDNYD KVHSF

References

[1]	"Nucleotide sequence of the DdeI restriction-modification system and characterization of the methylase protein." Sznyter L.A., Slatko B., Moran L., O'Donnell K.H., Brooks J.E. Nucleic Acids Res. 15:8249-8266(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00449 Genomic DNA. Translation: CAA68505.1.
3D structure databases
ProteinModelPortal	P05302.
ModBase	Search...
Protein family/group databases
REBASE	3357. M.DdeI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTD1_DESNO

Accession

Primary (citable) accession number: P05302

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1988
Last sequence update:	November 1, 1988
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program