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P05302 (MTD1_DESNO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase DdeI

Short name=M.DdeI
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase DdeI
Gene names
Name:ddeIM
Synonyms:ddeM
OrganismDesulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))
Taxonomic identifier52561 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfomicrobiaceaeDesulfomicrobium

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Modification methylase DdeI
PRO_0000087873

Regions

Domain1 – 373373SAM-dependent MTase C5-type

Sites

Active site761 By similarity

Sequences

Sequence LengthMass (Da)Tools
P05302 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 34DF5968E0414EED

FASTA41547,081
        10         20         30         40         50         60 
MNIIDLFAGC GGFSHGFKMA GYNSILAIEK DLWASQTYSF NNPNVSVITE DITTLDPGDL 

        70         80         90        100        110        120 
KISVSDVDGI IGGPPCQGFS LSGNRDQKDP RNSLFVDFVR FVKFFSPKFF VMENVLGILS 

       130        140        150        160        170        180 
MKTKSRQYVK DIIAEEFSNV GYKVCVIILN ACDYGVPQSR QRVFFIGLKS DRPLNQQILT 

       190        200        210        220        230        240 
PPSKVIESEY TSLEEAISDL PVIEAGEGGE VQDYPVAPRN KYQENMRKGS TCVYNHVAMR 

       250        260        270        280        290        300 
HTQRLVDRFA AIKFGQSVKH VSEEHSQRKR GDANSISGKV FSQNNMRPYP YKPCPTVAAS 

       310        320        330        340        350        360 
FQSNFIHPFY NRNFTAREGA RIQSFPDTYI FQGKRTTMSW EKHLSQYQQI GNAVPPLLAQ 

       370        380        390        400        410 
ALAERISWYF ENINLINDSN VSIKRMVQRS FMSQLNLENN VNVRQDDNYD KVHSF 

« Hide

References

[1]"Nucleotide sequence of the DdeI restriction-modification system and characterization of the methylase protein."
Sznyter L.A., Slatko B., Moran L., O'Donnell K.H., Brooks J.E.
Nucleic Acids Res. 15:8249-8266(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00449 Genomic DNA. Translation: CAA68505.1.

3D structure databases

ProteinModelPortalP05302.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3357. M.DdeI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTD1_DESNO
AccessionPrimary (citable) accession number: P05302
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries