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P05302

- MTD1_DESNO

UniProt

P05302 - MTD1_DESNO

Protein

Modification methylase DdeI

Gene

ddeIM

Organism
Desulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Nov 1988)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei76 – 761PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3357. M.DdeI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase DdeI (EC:2.1.1.37)
    Short name:
    M.DdeI
    Alternative name(s):
    Cytosine-specific methyltransferase DdeI
    Gene namesi
    Name:ddeIM
    Synonyms:ddeM
    OrganismiDesulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))
    Taxonomic identifieri52561 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfomicrobiaceaeDesulfomicrobium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415Modification methylase DdeIPRO_0000087873Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP05302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 373373SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05302-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIIDLFAGC GGFSHGFKMA GYNSILAIEK DLWASQTYSF NNPNVSVITE    50
    DITTLDPGDL KISVSDVDGI IGGPPCQGFS LSGNRDQKDP RNSLFVDFVR 100
    FVKFFSPKFF VMENVLGILS MKTKSRQYVK DIIAEEFSNV GYKVCVIILN 150
    ACDYGVPQSR QRVFFIGLKS DRPLNQQILT PPSKVIESEY TSLEEAISDL 200
    PVIEAGEGGE VQDYPVAPRN KYQENMRKGS TCVYNHVAMR HTQRLVDRFA 250
    AIKFGQSVKH VSEEHSQRKR GDANSISGKV FSQNNMRPYP YKPCPTVAAS 300
    FQSNFIHPFY NRNFTAREGA RIQSFPDTYI FQGKRTTMSW EKHLSQYQQI 350
    GNAVPPLLAQ ALAERISWYF ENINLINDSN VSIKRMVQRS FMSQLNLENN 400
    VNVRQDDNYD KVHSF 415
    Length:415
    Mass (Da):47,081
    Last modified:November 1, 1988 - v1
    Checksum:i34DF5968E0414EED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00449 Genomic DNA. Translation: CAA68505.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00449 Genomic DNA. Translation: CAA68505.1 .

    3D structure databases

    ProteinModelPortali P05302.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3357. M.DdeI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the DdeI restriction-modification system and characterization of the methylase protein."
      Sznyter L.A., Slatko B., Moran L., O'Donnell K.H., Brooks J.E.
      Nucleic Acids Res. 15:8249-8266(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiMTD1_DESNO
    AccessioniPrimary (citable) accession number: P05302
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1988
    Last sequence update: November 1, 1988
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3