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P05301 (T2D1_DESNO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme DdeI

Short name=R.DdeI
EC=3.1.21.4
Alternative name(s):
Endonuclease DdeI
Type II restriction enzyme DdeI
Gene names
Name:ddeIR
Synonyms:ddeR
OrganismDesulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))
Taxonomic identifier52561 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfomicrobiaceaeDesulfomicrobium

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTNAG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240Type-2 restriction enzyme DdeI
PRO_0000077299

Sequences

Sequence LengthMass (Da)Tools
P05301 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: AE0A7821F2F41356

FASTA24027,808
        10         20         30         40         50         60 
MKAATDQELR KLIVLYNNVM EVMEHDAAKS MRDDNRAYGG FVRAAKGKIQ ELITERLVRT 

        70         80         90        100        110        120 
VWDVEMGENP ERLSINSKKI KIPILRSYVD SINDENLKKY ISSNILKYSY GLSVDKHVFI 

       130        140        150        160        170        180 
DNKFVLGIEC KAYTENAMLK RILVDFYLLK TKFPKLNCFL FQLESQLGGD YSECNKFPIG 

       190        200        210        220        230        240 
SYPTRTIMSY FKNVDLNIVT LLEGERKVDR PINKPQFFKP LKVEHLEVAI GYLQESLSEI 

« Hide

References

[1]"Nucleotide sequence of the DdeI restriction-modification system and characterization of the methylase protein."
Sznyter L.A., Slatko B., Moran L., O'Donnell K.H., Brooks J.E.
Nucleic Acids Res. 15:8249-8266(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y00449 Genomic DNA. Translation: CAA68504.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE770. DdeI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2D1_DESNO
AccessionPrimary (citable) accession number: P05301
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries