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Protein

Type-2 restriction enzyme DdeI

Gene

ddeIR

Organism
Desulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence CTNAG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi770. DdeI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme DdeI (EC:3.1.21.4)
Short name:
R.DdeI
Alternative name(s):
Endonuclease DdeI
Type II restriction enzyme DdeI
Gene namesi
Name:ddeIR
Synonyms:ddeR
OrganismiDesulfomicrobium norvegicum (strain DSM 1741 / NCIMB 8310) (Desulfovibrio baculatus (strain Norway 4)) (Desulfovibrio desulfuricans (strain Norway 4))
Taxonomic identifieri52561 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfovibrionalesDesulfomicrobiaceaeDesulfomicrobium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Type-2 restriction enzyme DdeIPRO_0000077299Add
BLAST

Sequencei

Sequence statusi: Complete.

P05301-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKAATDQELR KLIVLYNNVM EVMEHDAAKS MRDDNRAYGG FVRAAKGKIQ
60 70 80 90 100
ELITERLVRT VWDVEMGENP ERLSINSKKI KIPILRSYVD SINDENLKKY
110 120 130 140 150
ISSNILKYSY GLSVDKHVFI DNKFVLGIEC KAYTENAMLK RILVDFYLLK
160 170 180 190 200
TKFPKLNCFL FQLESQLGGD YSECNKFPIG SYPTRTIMSY FKNVDLNIVT
210 220 230 240
LLEGERKVDR PINKPQFFKP LKVEHLEVAI GYLQESLSEI
Length:240
Mass (Da):27,808
Last modified:November 1, 1988 - v1
Checksum:iAE0A7821F2F41356
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00449 Genomic DNA. Translation: CAA68504.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00449 Genomic DNA. Translation: CAA68504.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi770. DdeI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the DdeI restriction-modification system and characterization of the methylase protein."
    Sznyter L.A., Slatko B., Moran L., O'Donnell K.H., Brooks J.E.
    Nucleic Acids Res. 15:8249-8266(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT2D1_DESNO
AccessioniPrimary (citable) accession number: P05301
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1988
Last sequence update: November 1, 1988
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.