Reviewed, UniProtKB/Swiss-Prot P05225 (CAER5_XENLA)

Names and origin

Protein names	Recommended name:     Preprocaerulein clone PXC202 Cleaved into the following chain:     1- Recommended name:             Caerulein
Organism	Xenopus laevis (African clawed frog)
Taxonomic identifier	8355 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	187 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The pharmacological activities of caerulein are quite similar to the physiological activities of gastrin and related peptides.
Subcellular location	Secreted.
Tissue specificity	Expressed by the skin glands.
Sequence similarities	Belongs to the gastrin/cholecystokinin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat
Molecular function	Amphibian defense peptide
PTM	Amidation Cleavage on pair of basic residues Sulfation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	‹1 – 9	›9			PRO_0000010522
	Peptide	10 – 19	10	Caerulein		PRO_0000010523
	Propeptide	23 – 73	51			PRO_0000010524
	Peptide	74 – 83	10	Caerulein		PRO_0000010525
	Propeptide	87 – 137	51			PRO_0000010526
	Peptide	138 – 147	10	Caerulein		PRO_0000010527
	Propeptide	151 – 152	2			PRO_0000010528
	Peptide	153 – 162	10	Caerulein		PRO_0000010529
	Propeptide	166 – 187	22			PRO_0000010530
Amino acid modifications
	Modified residue	13	1	Sulfotyrosine
	Modified residue	19	1	Phenylalanine amide
	Modified residue	77	1	Sulfotyrosine
	Modified residue	83	1	Phenylalanine amide
	Modified residue	141	1	Sulfotyrosine
	Modified residue	147	1	Phenylalanine amide
	Modified residue	156	1	Sulfotyrosine
	Modified residue	162	1	Phenylalanine amide
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	187	1

Sequences

	Sequence		Length	Mass (Da)
	P05225-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 15738A4F3025E392	FASTA	187	21,242
	10 20 30 40 50 60 NDERRFADGQ QDYTGWMDFG RRDDEDDVNE RDVRGFGSFL GKALKAALKI GANALGGSPQ 70 80 90 100 110 120 QREANDERRF ADGQQDYTGW MDFGRRDDED DVNERDVRGF GSFLGKALKA ALKIGANALG 130 140 150 160 170 180 GSLQQREVND ERRFADGQQD YTGWMDFGRR DGQQDYTGWM DFGRRDDEDD VHERDVRGFG SFLGKAL

References

[1]	"An unusual repetitive structure of caerulein mRNA from the skin of Xenopus laevis." Wakabayashi T., Kato H., Tachibana S. Gene 31:295-299(1984) [PubMed: 6526274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skin.
[2]	"Presence of caerulein in extracts of the skin of Leptodactylus pentadactylus labyrinthicus and of Xenopus laevis." Anastasi A., Bertaccini G., Cei J.M., de Daro G., Erspamer V., Impicciatore M., Roseghini M. Br. J. Pharmacol. 38:221-228(1970) [PubMed: 5413288] [Abstract] Cited for: PROTEIN SEQUENCE OF CAERULEIN. Tissue: Skin secretion.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M12453 mRNA. Translation: AAA49689.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P05225.
Family and domain databases
InterPro	IPR001651. Gastrin. IPR013152. Gastrin/cholecystokinin_CS. [Graphical view]
Pfam	PF00918. Gastrin. 2 hits. [Graphical view]
SMART	SM00029. GASTRIN. 4 hits. [Graphical view]
PROSITE	PS00259. GASTRIN. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAER5_XENLA

Accession

Primary (citable) accession number: P05225

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project