Reviewed, UniProtKB/Swiss-Prot P05208 (ELA2A_MOUSE)

Names and origin

Protein names

Recommended name:
    Elastase-2A
    EC=3.4.21.71
Alternative name(s):
    Elastase-2

Gene names

Name:	Ela2a
Synonyms:	Ela-2, Ela2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts upon elastin.
Catalytic activity	Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16
	Propeptide	17 – 30	14	Activation peptide		PRO_0000027687
	Chain	31 – 271	241	Elastase-2A		PRO_0000027688
Regions
	Domain	31 – 269	239	Peptidase S1
Sites
	Active site	75	1	Charge relay system By similarity
	Active site	123	1	Charge relay system By similarity
	Active site	218	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	60 ↔ 76		By similarity
	Disulfide bond	157 ↔ 224		By similarity
	Disulfide bond	188 ↔ 204		By similarity
	Disulfide bond	214 ↔ 245		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05208-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: FA542AE38FED3B4B	FASTA	271	28,914
	10 20 30 40 50 60 MIRTLLLSAL VAGALSCGYP TYEVEDDVSR VVGGQEATPN TWPWQVSLQV LSSGRWRHNC 70 80 90 100 110 120 GGSLVANNWV LTAAHCLSNY QTYRVLLGAH SLSNPGAGSA AVQVSKLVVH QRWNSQNVGN 130 140 150 160 170 180 GYDIALIKLA SPVTLSKNIQ TACLPPAGTI LPRNYVCYVT GWGLLQTNGN SPDTLRQGRL 190 200 210 220 230 240 LVVDYATCSS ASWWGSSVKS SMVCAGGDGV TSSCNGDSGG PLNCRASNGQ WQVHGIVSFG 250 260 270 SSLGCNYPRK PSVFTRVSNY IDWINSVMAR N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence organisation and transcriptional regulation of the mouse elastase II and trypsin genes." Stevenson B.J., Hagenbuechle O., Wellauer P.K. Nucleic Acids Res. 14:8307-8330(1986) [PubMed: 3641189] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04573 mRNA. Translation: CAA28242.1. X04576 Genomic DNA. Translation: CAA28244.1. BC026552 mRNA. Translation: AAH26552.1.
IPI	IPI00117315.
PIR	A25528.
RefSeq	NP_031945.1.
UniGene	Mm.45316
3D structure databases
HSSP	HSSP built from PDB template 1BRU based on UniProtKB P08419.
SMR	P05208. Positions 31-271.
ModBase	Search...
Protein family/group databases
MEROPS	S01.155.
Proteomic databases
PRIDE	P05208.
Genome annotation databases
Ensembl	ENSMUSG00000058579. Mus musculus. [Contig view]
GeneID	13706.
KEGG	mmu:13706.
Organism-specific databases
MGI	MGI:95316. Ela2a.
Phylogenomic databases
HOGENOM	P05208.
HOVERGEN	P05208.
OMA	P05208. CSSASWW.
Enzyme and pathway databases
BRENDA	3.4.21.71. 244.
Gene expression databases
ArrayExpress	P05208.
Bgee	P05208.
CleanEx	MM_ELA2.
GermOnline	ENSMUSG00000058579. Mus musculus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	284464.
SOURCE	Search...

Entry information

Entry name

ELA2A_MOUSE

Accession

Primary (citable) accession number: P05208

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)