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Protein

Chymotrypsin-like elastase family member 2A

Gene

Cela2a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts upon elastin.

Catalytic activityi

Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Charge relay systemBy similarity
Active sitei123 – 1231Charge relay systemBy similarity
Active sitei218 – 2181Charge relay systemBy similarity

GO - Molecular functioni

  • serine hydrolase activity Source: MGI
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • establishment of skin barrier Source: MGI
  • proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.155.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin-like elastase family member 2A (EC:3.4.21.71)
Alternative name(s):
Elastase-2
Elastase-2A
Gene namesi
Name:Cela2a
Synonyms:Ela-2, Ela2, Ela2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:95316. Cela2a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Add
BLAST
Propeptidei17 – 3014Activation peptidePRO_0000027687Add
BLAST
Chaini31 – 271241Chymotrypsin-like elastase family member 2APRO_0000027688Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi60 ↔ 76PROSITE-ProRule annotation
Disulfide bondi157 ↔ 224PROSITE-ProRule annotation
Disulfide bondi188 ↔ 204PROSITE-ProRule annotation
Disulfide bondi214 ↔ 245PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

MaxQBiP05208.
PaxDbiP05208.
PRIDEiP05208.

PTM databases

PhosphoSiteiP05208.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

BgeeiP05208.
CleanExiMM_ELA2.
GenevisibleiP05208. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099539.

Structurei

3D structure databases

ProteinModelPortaliP05208.
SMRiP05208. Positions 31-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 269239Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
InParanoidiP05208.
KOiK01346.
OMAiASNGQWQ.
OrthoDBiEOG75B84T.
PhylomeDBiP05208.
TreeFamiTF330455.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRTLLLSAL VAGALSCGYP TYEVEDDVSR VVGGQEATPN TWPWQVSLQV
60 70 80 90 100
LSSGRWRHNC GGSLVANNWV LTAAHCLSNY QTYRVLLGAH SLSNPGAGSA
110 120 130 140 150
AVQVSKLVVH QRWNSQNVGN GYDIALIKLA SPVTLSKNIQ TACLPPAGTI
160 170 180 190 200
LPRNYVCYVT GWGLLQTNGN SPDTLRQGRL LVVDYATCSS ASWWGSSVKS
210 220 230 240 250
SMVCAGGDGV TSSCNGDSGG PLNCRASNGQ WQVHGIVSFG SSLGCNYPRK
260 270
PSVFTRVSNY IDWINSVMAR N
Length:271
Mass (Da):28,914
Last modified:August 13, 1987 - v1
Checksum:iFA542AE38FED3B4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04573 mRNA. Translation: CAA28242.1.
X04576 Genomic DNA. Translation: CAA28244.1.
BC026552 mRNA. Translation: AAH26552.1.
CCDSiCCDS18884.1.
PIRiA25528.
RefSeqiNP_031945.1. NM_007919.2.
UniGeneiMm.45316.

Genome annotation databases

EnsembliENSMUST00000102481; ENSMUSP00000099539; ENSMUSG00000058579.
GeneIDi13706.
KEGGimmu:13706.
UCSCiuc008vpj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04573 mRNA. Translation: CAA28242.1.
X04576 Genomic DNA. Translation: CAA28244.1.
BC026552 mRNA. Translation: AAH26552.1.
CCDSiCCDS18884.1.
PIRiA25528.
RefSeqiNP_031945.1. NM_007919.2.
UniGeneiMm.45316.

3D structure databases

ProteinModelPortaliP05208.
SMRiP05208. Positions 31-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099539.

Protein family/group databases

MEROPSiS01.155.

PTM databases

PhosphoSiteiP05208.

Proteomic databases

MaxQBiP05208.
PaxDbiP05208.
PRIDEiP05208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102481; ENSMUSP00000099539; ENSMUSG00000058579.
GeneIDi13706.
KEGGimmu:13706.
UCSCiuc008vpj.1. mouse.

Organism-specific databases

CTDi63036.
MGIiMGI:95316. Cela2a.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
InParanoidiP05208.
KOiK01346.
OMAiASNGQWQ.
OrthoDBiEOG75B84T.
PhylomeDBiP05208.
TreeFamiTF330455.

Miscellaneous databases

ChiTaRSiCela2a. mouse.
NextBioi284464.
PROiP05208.
SOURCEiSearch...

Gene expression databases

BgeeiP05208.
CleanExiMM_ELA2.
GenevisibleiP05208. MM.

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence organisation and transcriptional regulation of the mouse elastase II and trypsin genes."
    Stevenson B.J., Hagenbuechle O., Wellauer P.K.
    Nucleic Acids Res. 14:8307-8330(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiCEL2A_MOUSE
AccessioniPrimary (citable) accession number: P05208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: May 11, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.