P05204 (HMGN2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-histone chromosomal protein HMG-17

Alternative name(s):
High mobility group nucleosome-binding domain-containing protein 2

Gene names

Name:	HMGN2
Synonyms:	HMG17

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation By similarity.
Subcellular location	Nucleus. Cytoplasm. Note: Cytoplasmic enrichment upon phosphorylation. Ref.7
Post-translational modification	Phosphorylation favors cytoplasmic localization.
Sequence similarities	Belongs to the HMGN family.
Mass spectrometry	Molecular mass is 9261.5 Da from positions 2 - 90. Determined by ESI. Ref.7 Molecular mass is 9341.3±1.9 Da from positions 2 - 90. Determined by ESI. Ref.7 Molecular mass is 9421.5 Da from positions 2 - 90. Determined by ESI. Ref.7

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	chromatin organization Non-traceable author statement. Source: UniProtKB regulation of transcription, DNA-dependent Non-traceable author statement. Source: UniProtKB
Cellular_component	chromatin Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Non-traceable author statement. Source: UniProtKB
Molecular_function	DNA binding Non-traceable author statement. Source: UniProtKB nucleosomal DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GRB2	P62993	1	EBI-1758689,EBI-401755

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 90	89	Non-histone chromosomal protein HMG-17		PRO_0000206697
Amino acid modifications
	Modified residue	25	1	Phosphoserine Ref.7
	Modified residue	29	1	Phosphoserine Ref.7
	Modified residue	82	1	N6-acetyllysine Ref.8
Natural variations
	Natural variant	7	1	E → K in variant H17. Ref.6		VAR_003716
Experimental info
	Sequence conflict	5	1	K → N Ref.1
	Sequence conflict	26 – 27	2	AR → SA AA sequence Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P05204 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: B1C58E52200422C8	FASTA	90	9,393
	10 20 30 40 50 60 MPKRKAEGDA KGDKAKVKDE PQRRSARLSA KPAPPKPEPK PKKAPAKKGE KVPKGKKGKA 70 80 90 DAGKEGNNPA ENGDAKTDQA QKAEGAGDAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chromosomal protein HMG-17. Complete human cDNA sequence and evidence for a multigene family." Landsman D., Soares N., Gonzalez F.J., Bustin M. J. Biol. Chem. 261:7479-7484(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human non-histone chromosomal protein HMG-17: identification, characterization, chromosome localization and RFLPs of a functional gene from the large multigene family." Landsman D., McBride O.W., Bustin M. Nucleic Acids Res. 17:2301-2314(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Colon, Eye, Lung, Lymph, Mammary gland, Muscle, Salivary gland and Skin.
[6]	"Comparison of multiple forms of the high mobility group I proteins in rodent and human cells. Identification of the human high mobility group I-C protein." Giancotti V., Bandiera A., Buratti E., Fusco A., Marzari R., Coles B., Goodwin G.H. Eur. J. Biochem. 198:211-216(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-28, VARIANT LYS-7. Tissue: Peripheral blood lymphocyte.
[7]	"Phosphorylation and subcellular redistribution of high mobility group proteins 14 and 17, analyzed by mass spectrometry." Louie D.F., Gloor K.K., Galasinski S.C., Resing K.A., Ahn N.G. Protein Sci. 9:170-179(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-31, PHOSPHORYLATION AT SER-25 AND SER-29, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[8]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-82, MASS SPECTROMETRY.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12623 mRNA. Translation: AAA52678.1. X13546 Genomic DNA. Translation: CAA31898.1. CR542122 mRNA. Translation: CAG46919.1. AL513365 Genomic DNA. Translation: CAI21489.1. BC000378 mRNA. Translation: AAH00378.1. BC003050 mRNA. Translation: AAH03050.1. BC014644 mRNA. Translation: AAH14644.2. BC032140 mRNA. Translation: AAH32140.2. BC070297 mRNA. Translation: AAH70297.1. BC071707 mRNA. Translation: AAH71707.1. BC072010 mRNA. Translation: AAH72010.1. BC072011 mRNA. Translation: AAH72011.1. BC075837 mRNA. Translation: AAH75837.1. BC081567 mRNA. Translation: AAH81567.1. BC110390 mRNA. Translation: AAI10391.1.
IPI	IPI00217950.
PIR	S03700.
RefSeq	NP_005508.1. NM_005517.3.
UniGene	Hs.181163.
3D structure databases
DisProt	DP00039.
ProteinModelPortal	P05204.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05204. 3 interactions.
STRING	9606.ENSP00000355228.
PTM databases
PhosphoSite	P05204.
Polymorphism databases
DMDM	123106.
Proteomic databases
PaxDb	P05204.
PRIDE	P05204.
Protocols and materials databases
DNASU	3151.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000361427; ENSP00000355228; ENSG00000198830.
GeneID	3151.
KEGG	hsa:3151.
UCSC	uc001bmp.4. human.
Organism-specific databases
CTD	3151.
GeneCards	GC01P026800.
HGNC	HGNC:4986. HMGN2.
HPA	CAB018371. HPA048518.
MIM	163910. gene.
neXtProt	NX_P05204.
PharmGKB	PA35089.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG44189.
HOGENOM	HOG000116395.
HOVERGEN	HBG073479.
InParanoid	P05204.
KO	K11300.
OMA	SAXKGEK.
OrthoDB	EOG47WNQG.
Gene expression databases
Bgee	P05204.
CleanEx	HS_HMGN2.
Genevestigator	P05204.
GermOnline	ENSG00000196819. Homo sapiens. ENSG00000198830. Homo sapiens.
Family and domain databases
InterPro	IPR000079. HMGN_fam. [Graphical view]
PANTHER	PTHR23087. PTHR23087. 1 hit.
Pfam	PF01101. HMG14_17. 1 hit. [Graphical view]
PRINTS	PR00925. NONHISHMG17.
SMART	SM00527. HMG17. 1 hit. [Graphical view]
PROSITE	PS00355. HMG14_17. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HMGN2. human.
GenomeRNAi	3151.
NextBio	12488.
SOURCE	Search...

Entry information

Entry name

HMGN2_HUMAN

Accession

Primary (citable) accession number: P05204
Secondary accession number(s): Q0VGD5, Q6FGI5, Q96C64

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 130 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.