Reviewed, UniProtKB/Swiss-Prot P05195 (3DHQ_NEUCR)

Names and origin

Protein names

Recommended name:
    Catabolic 3-dehydroquinase
    EC=4.2.1.10
Alternative name(s):
    3-dehydroquinate dehydratase

Gene names

Name:	qa-2
ORF Names:	NCU06023

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is involved in the catabolism of quinate.
Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O.
Pathway	Aromatic compound metabolism; 3,4-dihydroxybenzoate biosynthesis; 3,4-dihydroxybenzoate from 3-dehydroquinate: step 1/2.
Subunit structure	Homododecamer Probable.
Sequence similarities	Belongs to the type-II 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Quinate metabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quinate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 173	173	Catabolic 3-dehydroquinase		PRO_0000159948
Regions
	Region	129 – 130	2	Substrate binding By similarity
Sites
	Active site	26	1	Proton acceptor By similarity
	Active site	128	1	Proton donor By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	108	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Site	21	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	25 – 29	5	IYGST → STAQS in CAA24237. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P05195-1 [UniParc]. Last modified October 1, 1989. Version 2. Checksum: 6A6EC7594DD46F1B	FASTA	173	18,272
	10 20 30 40 50 60 MASPRHILLI NGPNLNLLGT REPQIYGSTT LHDIEQASQT LASSLGLRLT TFQSNHEGAI 70 80 90 100 110 120 IDRIHQAAGF VPSPPSPSPS SAATTTEAGL GPGDKVSAII INPGAYTHTS IGIRDALLGT 130 140 150 160 170 GIPFVEVHVS NVHAREAFRH HSYLSDKAVA VICGLGPFGY SAALDFLGRH MKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence, organization and regulation of the qa gene cluster of Neurospora crassa." Geever R.F., Huiet L., Baum J.A., Tyler B.M., Patel V.B., Rutledge B.J., Case M.E., Giles N.H. J. Mol. Biol. 207:15-34(1989) [PubMed: 2525625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"5'-Untranslated sequences of two structural genes in the qa gene cluster of Neurospora crassa." Alton N.K., Buxton F., Patel V., Giles N.H., Vapnek D. Proc. Natl. Acad. Sci. U.S.A. 79:1955-1959(1982) [PubMed: 6210913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-124.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X14603 Genomic DNA. Translation: CAA32749.1. V00869 Genomic DNA. Translation: CAA24237.1. AABX02000021 Genomic DNA. Translation: EAA30377.1.
PIR	A31277. S04251.
RefSeq	XP_959613.1.
3D structure databases
HSSP	HSSP built from PDB template 1GU1 based on UniProtKB P15474.
ModBase	Search...
Protein-protein interaction databases
STRING	P05195.
Genome annotation databases
GeneID	3875772.
KEGG	ncr:NCU06023.
NMPDR	fig|5141.1.peg.3811.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-009771-MON.
BRENDA	4.2.1.10. 266.
Family and domain databases
InterPro	IPR001874. DHquinase_II. IPR018509. DHquinase_II_CS. [Graphical view]
PANTHER	PTHR21272. DHquinase_II. 1 hit.
Pfam	PF01220. DHquinase_II. 1 hit. [Graphical view]
PIRSF	PIRSF001399. DHquinase_II. 1 hit.
ProDom	PD004527. DHquinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01088. aroQ. 1 hit.
PROSITE	PS01029. DEHYDROQUINASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3DHQ_NEUCR

Accession

Primary (citable) accession number: P05195
Secondary accession number(s): Q7RVA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	October 1, 1989
Last modified:	September 1, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)