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Protein

Alkaline phosphatase, placental type

Gene

ALPP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Magnesium4 Publications1
Metal bindingi64Zinc 14 Publications1
Active sitei114Phosphoserine intermediate1
Metal bindingi114Zinc 14 Publications1
Metal bindingi177Magnesium4 Publications1
Metal bindingi333Magnesium4 Publications1
Metal bindingi338Zinc 24 Publications1
Metal bindingi342Zinc 2; via tele nitrogen4 Publications1
Metal bindingi379Zinc 14 Publications1
Metal bindingi380Zinc 1; via tele nitrogen4 Publications1
Metal bindingi454Zinc 2; via tele nitrogen4 Publications1

GO - Molecular functioni

  • alkaline phosphatase activity Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS08818-MONOMER.
BRENDAi3.1.3.1. 2681.
ReactomeiR-HSA-6811438. Intra-Golgi traffic.
SABIO-RKP05187.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, placental type (EC:3.1.3.1)
Alternative name(s):
Alkaline phosphatase Regan isozyme
Placental alkaline phosphatase 1
Short name:
PLAP-1
Gene namesi
Name:ALPP
Synonyms:PLAP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:439. ALPP.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei513 – 529HelicalAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi250.
OpenTargetsiENSG00000163283.
PharmGKBiPA24730.

Chemistry databases

ChEMBLiCHEMBL4458.

Polymorphism and mutation databases

BioMutaiALPP.
DMDMi130737.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000002403123 – 506Alkaline phosphatase, placental typeAdd BLAST484
PropeptideiPRO_0000024032507 – 535Removed in mature formAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi143 ↔ 2055 Publications
Glycosylationi144N-linked (GlcNAc...)3 Publications1
Glycosylationi271N-linked (GlcNAc...)3 Publications1
Disulfide bondi489 ↔ 4965 Publications
Lipidationi506GPI-anchor amidated aspartate1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

EPDiP05187.
MaxQBiP05187.
PaxDbiP05187.
PeptideAtlasiP05187.
PRIDEiP05187.

PTM databases

DEPODiP05187.
iPTMnetiP05187.
PhosphoSitePlusiP05187.

Expressioni

Tissue specificityi

Detected in placenta (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000163283.
CleanExiHS_ALPP.
GenevisibleiP05187. HS.

Organism-specific databases

HPAiCAB026327.
HPA038764.
HPA038765.
HPA051699.

Interactioni

Subunit structurei

Homodimer.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP4-12Q9BQ663EBI-1211484,EBI-739863
KRTAP5-9P263715EBI-1211484,EBI-3958099

Protein-protein interaction databases

BioGridi106751. 24 interactors.
IntActiP05187. 27 interactors.
MINTiMINT-4054397.
STRINGi9606.ENSP00000375881.

Chemistry databases

BindingDBiP05187.

Structurei

Secondary structure

1535
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 29Combined sources4
Helixi31 – 47Combined sources17
Beta strandi56 – 63Combined sources8
Helixi68 – 81Combined sources14
Helixi93 – 95Combined sources3
Beta strandi97 – 103Combined sources7
Helixi114 – 123Combined sources10
Beta strandi132 – 134Combined sources3
Helixi143 – 145Combined sources3
Helixi154 – 160Combined sources7
Beta strandi164 – 172Combined sources9
Helixi176 – 179Combined sources4
Turni180 – 182Combined sources3
Helixi193 – 195Combined sources3
Helixi198 – 202Combined sources5
Helixi208 – 214Combined sources7
Beta strandi219 – 224Combined sources6
Helixi226 – 229Combined sources4
Helixi243 – 245Combined sources3
Beta strandi249 – 251Combined sources3
Helixi255 – 261Combined sources7
Beta strandi266 – 269Combined sources4
Helixi272 – 279Combined sources8
Beta strandi285 – 290Combined sources6
Beta strandi292 – 295Combined sources4
Helixi299 – 301Combined sources3
Turni304 – 306Combined sources3
Helixi310 – 321Combined sources12
Beta strandi328 – 334Combined sources7
Helixi337 – 342Combined sources6
Helixi346 – 366Combined sources21
Turni369 – 371Combined sources3
Beta strandi372 – 379Combined sources8
Beta strandi381 – 386Combined sources6
Beta strandi411 – 418Combined sources8
Helixi433 – 436Combined sources4
Beta strandi445 – 447Combined sources3
Beta strandi459 – 465Combined sources7
Helixi468 – 470Combined sources3
Beta strandi473 – 476Combined sources4
Helixi479 – 487Combined sources9
Helixi491 – 493Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EW2X-ray1.82A23-535[»]
1ZEBX-ray1.90A23-506[»]
1ZEDX-ray1.57A23-506[»]
1ZEFX-ray1.90A23-506[»]
2GLQX-ray1.60A23-506[»]
3MK0X-ray1.90A23-506[»]
3MK1X-ray1.57A23-506[»]
3MK2X-ray1.89A23-503[»]
ProteinModelPortaliP05187.
SMRiP05187.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05187.

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP05187.
KOiK01077.
OMAiLNAQPAY.
OrthoDBiEOG091G067H.
PhylomeDBiP05187.
TreeFamiTF323513.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05187-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGPCMLLLL LLLGLRLQLS LGIIPVEEEN PDFWNREAAE ALGAAKKLQP
60 70 80 90 100
AQTAAKNLII FLGDGMGVST VTAARILKGQ KKDKLGPEIP LAMDRFPYVA
110 120 130 140 150
LSKTYNVDKH VPDSGATATA YLCGVKGNFQ TIGLSAAARF NQCNTTRGNE
160 170 180 190 200
VISVMNRAKK AGKSVGVVTT TRVQHASPAG TYAHTVNRNW YSDADVPASA
210 220 230 240 250
RQEGCQDIAT QLISNMDIDV ILGGGRKYMF RMGTPDPEYP DDYSQGGTRL
260 270 280 290 300
DGKNLVQEWL AKRQGARYVW NRTELMQASL DPSVTHLMGL FEPGDMKYEI
310 320 330 340 350
HRDSTLDPSL MEMTEAALRL LSRNPRGFFL FVEGGRIDHG HHESRAYRAL
360 370 380 390 400
TETIMFDDAI ERAGQLTSEE DTLSLVTADH SHVFSFGGYP LRGSSIFGLA
410 420 430 440 450
PGKARDRKAY TVLLYGNGPG YVLKDGARPD VTESESGSPE YRQQSAVPLD
460 470 480 490 500
EETHAGEDVA VFARGPQAHL VHGVQEQTFI AHVMAFAACL EPYTACDLAP
510 520 530
PAGTTDAAHP GRSVVPALLP LLAGTLLLLE TATAP
Length:535
Mass (Da):57,954
Last modified:July 1, 1989 - v2
Checksum:i13C136679A70C76B
GO

Sequence cautioni

The sequence AAA51708 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66M → V in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti261 – 262AK → GE in AAA51706 (PubMed:3001717).Curated2
Sequence conflicti277Q → R in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti285T → A in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti324N → H in AAA51706 (PubMed:3001717).Curated1
Sequence conflicti389Y → C in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti394S → G in AAA51709 (PubMed:3461452).Curated1
Sequence conflicti396 – 397IF → FI in AAA51706 (PubMed:3001717).Curated2
Sequence conflicti401P → A in AAA51706 (PubMed:3001717).Curated1
Sequence conflicti436S → T (PubMed:3459156).Curated1

Polymorphismi

Placental ALP is highly polymorphic, there are at least three common alleles.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01741925P → L.2 PublicationsCorresponds to variant rs1130335dbSNPEnsembl.1
Natural variantiVAR_05052089I → L.1 PublicationCorresponds to variant rs13026692dbSNPEnsembl.1
Natural variantiVAR_050521231R → P.1 PublicationCorresponds to variant rs1048988dbSNPEnsembl.1
Natural variantiVAR_050522263R → H.1 PublicationCorresponds to variant rs2853378dbSNPEnsembl.1
Natural variantiVAR_050523451E → G.Corresponds to variant rs1048994dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19159 Genomic DNA. Translation: AAA51710.1.
M13077 mRNA. Translation: AAC97139.1.
M14169 mRNA. Translation: AAA51708.1. Different initiation.
M14170 mRNA. Translation: AAA51709.1.
AC068134 Genomic DNA. Translation: AAY24087.1.
BC009647 mRNA. Translation: AAH09647.1.
BC068501 mRNA. Translation: AAH68501.1.
BC094743 mRNA. Translation: AAH94743.1.
M12551 mRNA. Translation: AAA51706.1.
CCDSiCCDS2490.1.
PIRiA31074. PAHUA.
RefSeqiNP_001623.3. NM_001632.4.
UniGeneiHs.284255.

Genome annotation databases

EnsembliENST00000392027; ENSP00000375881; ENSG00000163283.
GeneIDi250.
KEGGihsa:250.
UCSCiuc002vsq.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Alkaline phosphatase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19159 Genomic DNA. Translation: AAA51710.1.
M13077 mRNA. Translation: AAC97139.1.
M14169 mRNA. Translation: AAA51708.1. Different initiation.
M14170 mRNA. Translation: AAA51709.1.
AC068134 Genomic DNA. Translation: AAY24087.1.
BC009647 mRNA. Translation: AAH09647.1.
BC068501 mRNA. Translation: AAH68501.1.
BC094743 mRNA. Translation: AAH94743.1.
M12551 mRNA. Translation: AAA51706.1.
CCDSiCCDS2490.1.
PIRiA31074. PAHUA.
RefSeqiNP_001623.3. NM_001632.4.
UniGeneiHs.284255.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EW2X-ray1.82A23-535[»]
1ZEBX-ray1.90A23-506[»]
1ZEDX-ray1.57A23-506[»]
1ZEFX-ray1.90A23-506[»]
2GLQX-ray1.60A23-506[»]
3MK0X-ray1.90A23-506[»]
3MK1X-ray1.57A23-506[»]
3MK2X-ray1.89A23-503[»]
ProteinModelPortaliP05187.
SMRiP05187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106751. 24 interactors.
IntActiP05187. 27 interactors.
MINTiMINT-4054397.
STRINGi9606.ENSP00000375881.

Chemistry databases

BindingDBiP05187.
ChEMBLiCHEMBL4458.

PTM databases

DEPODiP05187.
iPTMnetiP05187.
PhosphoSitePlusiP05187.

Polymorphism and mutation databases

BioMutaiALPP.
DMDMi130737.

Proteomic databases

EPDiP05187.
MaxQBiP05187.
PaxDbiP05187.
PeptideAtlasiP05187.
PRIDEiP05187.

Protocols and materials databases

DNASUi250.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000392027; ENSP00000375881; ENSG00000163283.
GeneIDi250.
KEGGihsa:250.
UCSCiuc002vsq.4. human.

Organism-specific databases

CTDi250.
DisGeNETi250.
GeneCardsiALPP.
HGNCiHGNC:439. ALPP.
HPAiCAB026327.
HPA038764.
HPA038765.
HPA051699.
MIMi171800. gene.
neXtProtiNX_P05187.
OpenTargetsiENSG00000163283.
PharmGKBiPA24730.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP05187.
KOiK01077.
OMAiLNAQPAY.
OrthoDBiEOG091G067H.
PhylomeDBiP05187.
TreeFamiTF323513.

Enzyme and pathway databases

BioCyciZFISH:HS08818-MONOMER.
BRENDAi3.1.3.1. 2681.
ReactomeiR-HSA-6811438. Intra-Golgi traffic.
SABIO-RKP05187.

Miscellaneous databases

ChiTaRSiALPP. human.
EvolutionaryTraceiP05187.
GeneWikiiPlacental_alkaline_phosphatase.
GenomeRNAii250.
PROiP05187.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163283.
CleanExiHS_ALPP.
GenevisibleiP05187. HS.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPB1_HUMAN
AccessioniPrimary (citable) accession number: P05187
Secondary accession number(s): P05188
, P06861, Q53S78, Q96DB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 190 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.