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P05185 (CP17A_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase

EC=1.14.99.9
EC=4.1.2.30
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17
Gene names
Name:CYP17A1
Synonyms:CYP17
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length509 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Catalytic activity

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactor

Heme group By similarity.

Enzyme regulation

Regulated predominantly by intracellular cAMP levels.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Membrane Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 509509Steroid 17-alpha-hydroxylase/17,20 lyase
PRO_0000051926

Sites

Metal binding4421Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict2401N → K in AAI10170. Ref.3
Sequence conflict4231T → A no nucleotide entry Ref.2
Sequence conflict4581R → W no nucleotide entry Ref.2
Sequence conflict5091P → S no nucleotide entry Ref.2

Sequences

Sequence LengthMass (Da)Tools
P05185 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 64B0E5537A474BB7

FASTA50957,244
        10         20         30         40         50         60 
MWLLLAVFLL TLAYLFWPKT KHSGAKYPRS LPSLPLVGSL PFLPRRGQQH KNFFKLQEKY 

        70         80         90        100        110        120 
GPIYSFRLGS KTTVMIGHHQ LAREVLLKKG KEFSGRPKVA TLDILSDNQK GIAFADHGAH 

       130        140        150        160        170        180 
WQLHRKLALN AFALFKDGNL KLEKIINQEA NVLCDFLATQ HGEAIDLSEP LSLAVTNIIS 

       190        200        210        220        230        240 
FICFNFSFKN EDPALKAIQN VNDGILEVLS KEVLLDIFPV LKIFPSKAME KMKGCVQTRN 

       250        260        270        280        290        300 
ELLNEILEKC QENFSSDSIT NLLHILIQAK VNADNNNAGP DQDSKLLSNR HMLATIGDIF 

       310        320        330        340        350        360 
GAGVETTTSV IKWIVAYLLH HPSLKKRIQD DIDQIIGFNR TPTISDRNRL VLLEATIREV 

       370        380        390        400        410        420 
LRIRPVAPTL IPHKAVIDSS IGDLTIDKGT DVVVNLWALH HSEKEWQHPD LFMPERFLDP 

       430        440        450        460        470        480 
TGTQLISPSL SYLPFGAGPR SCVGEMLARQ ELFLFMSRLL QRFNLEIPDD GKLPSLEGHA 

       490        500 
SLVLQIKPFK VKIEVRQAWK EAQAEGSTP 

« Hide

References

« Hide 'large scale' references
[1]"Bovine adrenocortical cytochrome P-450(17 alpha). Regulation of gene expression by ACTH and elucidation of primary sequence."
Zuber M.X., John M.E., Okamura T., Simpson E.R., Waterman M.R.
J. Biol. Chem. 261:2475-2482(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural characterization of the bovine CYP17 (17 alpha-hydroxylase) gene."
Bhasker C.R., Adler B.S., Dee A., John M.E., Kagimoto M., Zuber M.X., Ahlgren R., Wang X.D., Simpson E.R., Waterman M.R.
Arch. Biochem. Biophys. 271:479-487(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]NIH - Mammalian Gene Collection (MGC) project
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M12547 mRNA. Translation: AAA30485.1.
BC110169 mRNA. Translation: AAI10170.1.
PIRS04346.
RefSeqNP_776729.1. NM_174304.2.
XP_005200162.1. XM_005200105.1.
UniGeneBt.89153.

3D structure databases

ProteinModelPortalP05185.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000019061.

Proteomic databases

PRIDEP05185.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID101908425.
281739.
KEGGbta:101908425.
bta:281739.

Organism-specific databases

CTD1586.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036991.
HOVERGENHBG106944.
InParanoidP05185.
KOK00512.

Enzyme and pathway databases

UniPathwayUPA00062.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805659.

Entry information

Entry nameCP17A_BOVIN
AccessionPrimary (citable) accession number: P05185
Secondary accession number(s): Q2YDL3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways