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Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

CYP17A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.

Catalytic activityi

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactori

hemeBy similarity

Enzyme regulationi

Regulated predominantly by intracellular cAMP levels.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi442 – 4421Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name:
Cytochrome P450c17
Gene namesi
Name:CYP17A1
Synonyms:CYP17
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051926Add
BLAST

Proteomic databases

PRIDEiP05185.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019061.

Structurei

3D structure databases

ProteinModelPortaliP05185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP05185.
KOiK00512.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05185-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLLLAVFLL TLAYLFWPKT KHSGAKYPRS LPSLPLVGSL PFLPRRGQQH
60 70 80 90 100
KNFFKLQEKY GPIYSFRLGS KTTVMIGHHQ LAREVLLKKG KEFSGRPKVA
110 120 130 140 150
TLDILSDNQK GIAFADHGAH WQLHRKLALN AFALFKDGNL KLEKIINQEA
160 170 180 190 200
NVLCDFLATQ HGEAIDLSEP LSLAVTNIIS FICFNFSFKN EDPALKAIQN
210 220 230 240 250
VNDGILEVLS KEVLLDIFPV LKIFPSKAME KMKGCVQTRN ELLNEILEKC
260 270 280 290 300
QENFSSDSIT NLLHILIQAK VNADNNNAGP DQDSKLLSNR HMLATIGDIF
310 320 330 340 350
GAGVETTTSV IKWIVAYLLH HPSLKKRIQD DIDQIIGFNR TPTISDRNRL
360 370 380 390 400
VLLEATIREV LRIRPVAPTL IPHKAVIDSS IGDLTIDKGT DVVVNLWALH
410 420 430 440 450
HSEKEWQHPD LFMPERFLDP TGTQLISPSL SYLPFGAGPR SCVGEMLARQ
460 470 480 490 500
ELFLFMSRLL QRFNLEIPDD GKLPSLEGHA SLVLQIKPFK VKIEVRQAWK

EAQAEGSTP
Length:509
Mass (Da):57,244
Last modified:August 13, 1987 - v1
Checksum:i64B0E5537A474BB7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti240 – 2401N → K in AAI10170 (Ref. 3) Curated
Sequence conflicti423 – 4231T → A no nucleotide entry (PubMed:2543297).Curated
Sequence conflicti458 – 4581R → W no nucleotide entry (PubMed:2543297).Curated
Sequence conflicti509 – 5091P → S no nucleotide entry (PubMed:2543297).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12547 mRNA. Translation: AAA30485.1.
BC110169 mRNA. Translation: AAI10170.1.
PIRiS04346.
RefSeqiNP_776729.1. NM_174304.2.
XP_005200162.1. XM_005200105.2.
UniGeneiBt.89153.

Genome annotation databases

GeneIDi101908425.
281739.
KEGGibta:101908425.
bta:281739.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12547 mRNA. Translation: AAA30485.1.
BC110169 mRNA. Translation: AAI10170.1.
PIRiS04346.
RefSeqiNP_776729.1. NM_174304.2.
XP_005200162.1. XM_005200105.2.
UniGeneiBt.89153.

3D structure databases

ProteinModelPortaliP05185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019061.

Proteomic databases

PRIDEiP05185.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101908425.
281739.
KEGGibta:101908425.
bta:281739.

Organism-specific databases

CTDi1586.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiP05185.
KOiK00512.

Enzyme and pathway databases

UniPathwayiUPA00062.

Miscellaneous databases

NextBioi20805659.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bovine adrenocortical cytochrome P-450(17 alpha). Regulation of gene expression by ACTH and elucidation of primary sequence."
    Zuber M.X., John M.E., Okamura T., Simpson E.R., Waterman M.R.
    J. Biol. Chem. 261:2475-2482(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiCP17A_BOVIN
AccessioniPrimary (citable) accession number: P05185
Secondary accession number(s): Q2YDL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 29, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.