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P05183

- CP3A2_RAT

UniProt

P05183 - CP3A2_RAT

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Protein

Cytochrome P450 3A2

Gene

Cyp3a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi443 – 4431Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. demethylase activity Source: RGD
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. monooxygenase activity Source: RGD
  6. testosterone 6-beta-hydroxylase activity Source: RGD

GO - Biological processi

  1. drug metabolic process Source: RGD
  2. oxidative demethylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP05183.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 3A2 (EC:1.14.14.1)
Alternative name(s):
CYPIIIA2
Cytochrome P450-PCN2
Cytochrome P450/6-beta-A
Testosterone 6-beta-hydroxylase
Gene namesi
Name:Cyp3a2
Synonyms:Cyp3a-2, Cyp3a11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi708379. Cyp3a2.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Cytochrome P450 3A2PRO_0000051784Add
BLAST

Proteomic databases

PaxDbiP05183.
PRIDEiP05183.

Expressioni

Tissue specificityi

Expressed in liver.2 Publications

Inductioni

By pregnenolone 16-alpha-carbonitrile (PNCN) and dexamethasone.3 Publications

Gene expression databases

GenevestigatoriP05183.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP05183.
SMRiP05183. Positions 30-497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000039127.
HOVERGENiHBG108567.
InParanoidiP05183.
KOiK07424.
PhylomeDBiP05183.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05183-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLLSALTLE TWVLLAVILV LLYRLGTHRH GIFKKQGIPG PKPLPFLGTV
60 70 80 90 100
LNYYKGLGRF DMECYKKYGK IWGLFDGQTP VFAIMDTEMI KNVLVKECFS
110 120 130 140 150
VFTNRRDFGP VGIMGKAVSV AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE
160 170 180 190 200
QYGDILVKYL KQEAETGKPV TMKKVFGAYS MDVITSTSFG VNVDSLNNPK
210 220 230 240 250
DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM FPKDSIAFFQ
260 270 280 290 300
KFVHRIKETR LDSKHKHRVD FLQLMLNAHN NSKDEVSHKA LSDVEIIAQS
310 320 330 340 350
VIFIFAGYET TSSTLSFVLY FLATHPDIQK KLQEEIDGAL PSKAPPTYDI
360 370 380 390 400
VMEMEYLDMV LNETLRLYPI GNRLERVCKK DIELDGLFIP KGSVVTIPTY
410 420 430 440 450
ALHHDPQHWP KPEEFHPERF SKENKGSIDP YVYLPFGNGP RNCIGMRFAL
460 470 480 490 500
MNMKLALTKV LQNFSFQPCK ETQIPLKLSR QAILEPEKPI VLKVLPRDAV

INGA
Length:504
Mass (Da):57,732
Last modified:July 15, 1999 - v2
Checksum:iC2C84AF736035AB2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 572GL → AV in CAA55887. (PubMed:2043144)Curated
Sequence conflicti429 – 4291D → H in AAA41051. (PubMed:3785219)Curated
Sequence conflicti445 – 4451G → D in AAA41051. (PubMed:3785219)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13646 mRNA. Translation: AAA41051.1.
X79319 Genomic DNA. Translation: CAA55887.1.
X79320 mRNA. Translation: CAA55888.1.
U09742 mRNA. Translation: AAA82168.1.
U09734
, U09725, U09726, U09727, U09728, U09729, U09730, U09731, U09732, U09733 Genomic DNA. Translation: AAB60492.1.
BC089765 mRNA. Translation: AAH89765.1.
PIRiA25222.
RefSeqiNP_695224.2. NM_153312.2.
UniGeneiRn.225885.

Genome annotation databases

GeneIDi266682.
KEGGirno:266682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13646 mRNA. Translation: AAA41051.1 .
X79319 Genomic DNA. Translation: CAA55887.1 .
X79320 mRNA. Translation: CAA55888.1 .
U09742 mRNA. Translation: AAA82168.1 .
U09734
, U09725 , U09726 , U09727 , U09728 , U09729 , U09730 , U09731 , U09732 , U09733 Genomic DNA. Translation: AAB60492.1 .
BC089765 mRNA. Translation: AAH89765.1 .
PIRi A25222.
RefSeqi NP_695224.2. NM_153312.2.
UniGenei Rn.225885.

3D structure databases

ProteinModelPortali P05183.
SMRi P05183. Positions 30-497.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P05183.
ChEMBLi CHEMBL3324.
DrugBanki DB08834. Tauroursodeoxycholic acid.

Proteomic databases

PaxDbi P05183.
PRIDEi P05183.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 266682.
KEGGi rno:266682.

Organism-specific databases

CTDi 266682.
RGDi 708379. Cyp3a2.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000039127.
HOVERGENi HBG108567.
InParanoidi P05183.
KOi K07424.
PhylomeDBi P05183.

Enzyme and pathway databases

SABIO-RK P05183.

Miscellaneous databases

NextBioi 624480.

Gene expression databases

Genevestigatori P05183.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Pregnenolone 16 alpha-carbonitrile-inducible P-450 gene family: gene conversion and differential regulation."
    Gonzalez F.J., Song B.-J., Hardwick J.P.
    Mol. Cell. Biol. 6:2969-2976(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A gene structure of testosterone 6 beta-hydroxylase (P450IIIA)."
    Miyata M., Nagata K., Yamazoe Y., Kato R.
    Biochem. Biophys. Res. Commun. 177:68-73(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "Structure of a gene and cDNA of a major constitutive form of testosterone 6 beta-hydroxylase (P450/6 beta A) encoding CYP3A2: comparison of the cDNA with P450PCN2."
    Miyata M., Nagata K., Shimada M., Yamazoe Y., Kato R.
    Arch. Biochem. Biophys. 314:351-359(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development."
    Telhada M.B., Pereira T.M., Lechner M.C.
    Arch. Biochem. Biophys. 298:715-725(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-36, INDUCTION.
    Tissue: Liver.
  6. "Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms."
    Nagata K., Gonzalez F.J., Yamazoe Y., Kato R.
    J. Biochem. 107:718-725(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-33, TISSUE SPECIFICITY, INDUCTION.
    Tissue: Liver.
  7. "Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats."
    Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E.
    Arch. Biochem. Biophys. 301:345-354(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-25, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiCP3A2_RAT
AccessioniPrimary (citable) accession number: P05183
Secondary accession number(s): Q5FVU5, Q64629, Q64672
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3