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P05183

- CP3A2_RAT

UniProt

P05183 - CP3A2_RAT

Protein

Cytochrome P450 3A2

Gene

Cyp3a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (15 Jul 1999)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi443 – 4431Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. demethylase activity Source: RGD
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. monooxygenase activity Source: RGD
    6. testosterone 6-beta-hydroxylase activity Source: RGD

    GO - Biological processi

    1. drug metabolic process Source: RGD
    2. oxidative demethylation Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP05183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 3A2 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIIA2
    Cytochrome P450-PCN2
    Cytochrome P450/6-beta-A
    Testosterone 6-beta-hydroxylase
    Gene namesi
    Name:Cyp3a2
    Synonyms:Cyp3a-2, Cyp3a11
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi708379. Cyp3a2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Cytochrome P450 3A2PRO_0000051784Add
    BLAST

    Proteomic databases

    PaxDbiP05183.
    PRIDEiP05183.

    Expressioni

    Tissue specificityi

    Expressed in liver.2 Publications

    Inductioni

    By pregnenolone 16-alpha-carbonitrile (PNCN) and dexamethasone.3 Publications

    Gene expression databases

    GenevestigatoriP05183.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliP05183.
    SMRiP05183. Positions 30-497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000039127.
    HOVERGENiHBG108567.
    KOiK07424.
    PhylomeDBiP05183.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR008072. Cyt_P450_E_CYP3A.
    IPR002402. Cyt_P450_E_grp-II.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00464. EP450II.
    PR01689. EP450IICYP3A.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05183-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLLSALTLE TWVLLAVILV LLYRLGTHRH GIFKKQGIPG PKPLPFLGTV    50
    LNYYKGLGRF DMECYKKYGK IWGLFDGQTP VFAIMDTEMI KNVLVKECFS 100
    VFTNRRDFGP VGIMGKAVSV AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE 150
    QYGDILVKYL KQEAETGKPV TMKKVFGAYS MDVITSTSFG VNVDSLNNPK 200
    DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM FPKDSIAFFQ 250
    KFVHRIKETR LDSKHKHRVD FLQLMLNAHN NSKDEVSHKA LSDVEIIAQS 300
    VIFIFAGYET TSSTLSFVLY FLATHPDIQK KLQEEIDGAL PSKAPPTYDI 350
    VMEMEYLDMV LNETLRLYPI GNRLERVCKK DIELDGLFIP KGSVVTIPTY 400
    ALHHDPQHWP KPEEFHPERF SKENKGSIDP YVYLPFGNGP RNCIGMRFAL 450
    MNMKLALTKV LQNFSFQPCK ETQIPLKLSR QAILEPEKPI VLKVLPRDAV 500
    INGA 504
    Length:504
    Mass (Da):57,732
    Last modified:July 15, 1999 - v2
    Checksum:iC2C84AF736035AB2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti56 – 572GL → AV in CAA55887. (PubMed:2043144)Curated
    Sequence conflicti429 – 4291D → H in AAA41051. (PubMed:3785219)Curated
    Sequence conflicti445 – 4451G → D in AAA41051. (PubMed:3785219)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13646 mRNA. Translation: AAA41051.1.
    X79319 Genomic DNA. Translation: CAA55887.1.
    X79320 mRNA. Translation: CAA55888.1.
    U09742 mRNA. Translation: AAA82168.1.
    U09734
    , U09725, U09726, U09727, U09728, U09729, U09730, U09731, U09732, U09733 Genomic DNA. Translation: AAB60492.1.
    BC089765 mRNA. Translation: AAH89765.1.
    PIRiA25222.
    RefSeqiNP_695224.2. NM_153312.2.
    UniGeneiRn.225885.

    Genome annotation databases

    GeneIDi266682.
    KEGGirno:266682.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13646 mRNA. Translation: AAA41051.1 .
    X79319 Genomic DNA. Translation: CAA55887.1 .
    X79320 mRNA. Translation: CAA55888.1 .
    U09742 mRNA. Translation: AAA82168.1 .
    U09734
    , U09725 , U09726 , U09727 , U09728 , U09729 , U09730 , U09731 , U09732 , U09733 Genomic DNA. Translation: AAB60492.1 .
    BC089765 mRNA. Translation: AAH89765.1 .
    PIRi A25222.
    RefSeqi NP_695224.2. NM_153312.2.
    UniGenei Rn.225885.

    3D structure databases

    ProteinModelPortali P05183.
    SMRi P05183. Positions 30-497.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P05183.
    ChEMBLi CHEMBL3324.

    Proteomic databases

    PaxDbi P05183.
    PRIDEi P05183.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 266682.
    KEGGi rno:266682.

    Organism-specific databases

    CTDi 266682.
    RGDi 708379. Cyp3a2.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000039127.
    HOVERGENi HBG108567.
    KOi K07424.
    PhylomeDBi P05183.

    Enzyme and pathway databases

    SABIO-RK P05183.

    Miscellaneous databases

    NextBioi 624480.

    Gene expression databases

    Genevestigatori P05183.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR008072. Cyt_P450_E_CYP3A.
    IPR002402. Cyt_P450_E_grp-II.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00464. EP450II.
    PR01689. EP450IICYP3A.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Pregnenolone 16 alpha-carbonitrile-inducible P-450 gene family: gene conversion and differential regulation."
      Gonzalez F.J., Song B.-J., Hardwick J.P.
      Mol. Cell. Biol. 6:2969-2976(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "A gene structure of testosterone 6 beta-hydroxylase (P450IIIA)."
      Miyata M., Nagata K., Yamazoe Y., Kato R.
      Biochem. Biophys. Res. Commun. 177:68-73(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    3. "Structure of a gene and cDNA of a major constitutive form of testosterone 6 beta-hydroxylase (P450/6 beta A) encoding CYP3A2: comparison of the cDNA with P450PCN2."
      Miyata M., Nagata K., Shimada M., Yamazoe Y., Kato R.
      Arch. Biochem. Biophys. 314:351-359(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    5. "Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development."
      Telhada M.B., Pereira T.M., Lechner M.C.
      Arch. Biochem. Biophys. 298:715-725(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-36, INDUCTION.
      Tissue: Liver.
    6. "Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms."
      Nagata K., Gonzalez F.J., Yamazoe Y., Kato R.
      J. Biochem. 107:718-725(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-33, TISSUE SPECIFICITY, INDUCTION.
      Tissue: Liver.
    7. "Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats."
      Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E.
      Arch. Biochem. Biophys. 301:345-354(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-25, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiCP3A2_RAT
    AccessioniPrimary (citable) accession number: P05183
    Secondary accession number(s): Q5FVU5, Q64629, Q64672
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: July 15, 1999
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3