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Protein

Cytochrome P450 3A2

Gene

Cyp3a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi443Iron (heme axial ligand)1

GO - Molecular functioni

  • aromatase activity Source: UniProtKB-EC
  • demethylase activity Source: RGD
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • monooxygenase activity Source: RGD
  • testosterone 6-beta-hydroxylase activity Source: RGD

GO - Biological processi

  • drug metabolic process Source: RGD
  • oxidative demethylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP05183.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 3A2 (EC:1.14.14.1)
Alternative name(s):
CYPIIIA2
Cytochrome P450-PCN2
Cytochrome P450/6-beta-A
Testosterone 6-beta-hydroxylase
Gene namesi
Name:Cyp3a2
Synonyms:Cyp3a-2, Cyp3a11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708379. Cyp3a2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3324.
DrugBankiDB08834. Tauroursodeoxycholic acid.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000517841 – 504Cytochrome P450 3A2Add BLAST504

Proteomic databases

PRIDEiP05183.

PTM databases

iPTMnetiP05183.
PhosphoSitePlusiP05183.

Expressioni

Tissue specificityi

Expressed in liver.2 Publications

Inductioni

By pregnenolone 16-alpha-carbonitrile (PNCN) and dexamethasone.3 Publications

Interactioni

Chemistry databases

BindingDBiP05183.

Structurei

3D structure databases

ProteinModelPortaliP05183.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOGENOMiHOG000039127.
HOVERGENiHBG108567.
InParanoidiP05183.
KOiK07424.
PhylomeDBiP05183.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05183-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLLSALTLE TWVLLAVILV LLYRLGTHRH GIFKKQGIPG PKPLPFLGTV
60 70 80 90 100
LNYYKGLGRF DMECYKKYGK IWGLFDGQTP VFAIMDTEMI KNVLVKECFS
110 120 130 140 150
VFTNRRDFGP VGIMGKAVSV AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE
160 170 180 190 200
QYGDILVKYL KQEAETGKPV TMKKVFGAYS MDVITSTSFG VNVDSLNNPK
210 220 230 240 250
DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM FPKDSIAFFQ
260 270 280 290 300
KFVHRIKETR LDSKHKHRVD FLQLMLNAHN NSKDEVSHKA LSDVEIIAQS
310 320 330 340 350
VIFIFAGYET TSSTLSFVLY FLATHPDIQK KLQEEIDGAL PSKAPPTYDI
360 370 380 390 400
VMEMEYLDMV LNETLRLYPI GNRLERVCKK DIELDGLFIP KGSVVTIPTY
410 420 430 440 450
ALHHDPQHWP KPEEFHPERF SKENKGSIDP YVYLPFGNGP RNCIGMRFAL
460 470 480 490 500
MNMKLALTKV LQNFSFQPCK ETQIPLKLSR QAILEPEKPI VLKVLPRDAV

INGA
Length:504
Mass (Da):57,732
Last modified:July 15, 1999 - v2
Checksum:iC2C84AF736035AB2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56 – 57GL → AV in CAA55887 (PubMed:2043144).Curated2
Sequence conflicti429D → H in AAA41051 (PubMed:3785219).Curated1
Sequence conflicti445G → D in AAA41051 (PubMed:3785219).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13646 mRNA. Translation: AAA41051.1.
X79319 Genomic DNA. Translation: CAA55887.1.
X79320 mRNA. Translation: CAA55888.1.
U09742 mRNA. Translation: AAA82168.1.
U09734
, U09725, U09726, U09727, U09728, U09729, U09730, U09731, U09732, U09733 Genomic DNA. Translation: AAB60492.1.
BC089765 mRNA. Translation: AAH89765.1.
PIRiA25222.
RefSeqiNP_695224.2. NM_153312.2.
UniGeneiRn.225885.

Genome annotation databases

GeneIDi266682.
KEGGirno:266682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13646 mRNA. Translation: AAA41051.1.
X79319 Genomic DNA. Translation: CAA55887.1.
X79320 mRNA. Translation: CAA55888.1.
U09742 mRNA. Translation: AAA82168.1.
U09734
, U09725, U09726, U09727, U09728, U09729, U09730, U09731, U09732, U09733 Genomic DNA. Translation: AAB60492.1.
BC089765 mRNA. Translation: AAH89765.1.
PIRiA25222.
RefSeqiNP_695224.2. NM_153312.2.
UniGeneiRn.225885.

3D structure databases

ProteinModelPortaliP05183.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP05183.
ChEMBLiCHEMBL3324.
DrugBankiDB08834. Tauroursodeoxycholic acid.

PTM databases

iPTMnetiP05183.
PhosphoSitePlusiP05183.

Proteomic databases

PRIDEiP05183.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi266682.
KEGGirno:266682.

Organism-specific databases

CTDi266682.
RGDi708379. Cyp3a2.

Phylogenomic databases

HOGENOMiHOG000039127.
HOVERGENiHBG108567.
InParanoidiP05183.
KOiK07424.
PhylomeDBiP05183.

Enzyme and pathway databases

SABIO-RKP05183.

Miscellaneous databases

PROiP05183.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP3A2_RAT
AccessioniPrimary (citable) accession number: P05183
Secondary accession number(s): Q5FVU5, Q64629, Q64672
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 15, 1999
Last modified: November 2, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.