Cytochrome P450 3A2 - Rattus norvegicus (Rat)

Contribute

Send feedback

Read comments (?) or add your own

P05183 (CP3A2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 110. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 3A2
EC=1.14.14.1

Alternative name(s):
CYPIIIA2
Cytochrome P450-PCN2
Cytochrome P450/6-beta-A
Testosterone 6-beta-hydroxylase

Gene names

Name:	Cyp3a2
Synonyms:	Cyp3a-2, Cyp3a11

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Expressed in liver. Ref.6 Ref.7
Induction	By pregnenolone 16-alpha-carbonitrile (PNCN) and dexamethasone. Ref.5 Ref.6 Ref.7
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	drug metabolic process Inferred from direct assay. Source: RGD oxidative demethylation Inferred from direct assay. Source: RGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC demethylase activity Inferred from direct assay. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro testosterone 6-beta-hydroxylase activity Inferred from mutant phenotype Ref.3. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 504

504

Cytochrome P450 3A2

PRO_0000051784

Sites

Metal binding

443

Iron (heme axial ligand)

Experimental info

Sequence conflict

56 – 57

GL → AV in CAA55887. Ref.2

Sequence conflict

429

D → H in AAA41051. Ref.1

Sequence conflict

445

G → D in AAA41051. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P05183 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: C2C84AF736035AB2
Show »

FASTA

504

57,732

        10         20         30         40         50         60 
MDLLSALTLE TWVLLAVILV LLYRLGTHRH GIFKKQGIPG PKPLPFLGTV LNYYKGLGRF 

        70         80         90        100        110        120 
DMECYKKYGK IWGLFDGQTP VFAIMDTEMI KNVLVKECFS VFTNRRDFGP VGIMGKAVSV 

       130        140        150        160        170        180 
AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE QYGDILVKYL KQEAETGKPV TMKKVFGAYS 

       190        200        210        220        230        240 
MDVITSTSFG VNVDSLNNPK DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM 

       250        260        270        280        290        300 
FPKDSIAFFQ KFVHRIKETR LDSKHKHRVD FLQLMLNAHN NSKDEVSHKA LSDVEIIAQS 

       310        320        330        340        350        360 
VIFIFAGYET TSSTLSFVLY FLATHPDIQK KLQEEIDGAL PSKAPPTYDI VMEMEYLDMV 

       370        380        390        400        410        420 
LNETLRLYPI GNRLERVCKK DIELDGLFIP KGSVVTIPTY ALHHDPQHWP KPEEFHPERF 

       430        440        450        460        470        480 
SKENKGSIDP YVYLPFGNGP RNCIGMRFAL MNMKLALTKV LQNFSFQPCK ETQIPLKLSR 

       490        500 
QAILEPEKPI VLKVLPRDAV INGA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pregnenolone 16 alpha-carbonitrile-inducible P-450 gene family: gene conversion and differential regulation." Gonzalez F.J., Song B.-J., Hardwick J.P. Mol. Cell. Biol. 6:2969-2976(1986) [PubMed: 3785219] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A gene structure of testosterone 6 beta-hydroxylase (P450IIIA)." Miyata M., Nagata K., Yamazoe Y., Kato R. Biochem. Biophys. Res. Commun. 177:68-73(1991) [PubMed: 2043144] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"Structure of a gene and cDNA of a major constitutive form of testosterone 6 beta-hydroxylase (P450/6 beta A) encoding CYP3A2: comparison of the cDNA with P450PCN2." Miyata M., Nagata K., Shimada M., Yamazoe Y., Kato R. Arch. Biochem. Biophys. 314:351-359(1994) [PubMed: 7979376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[5]	"Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development." Telhada M.B., Pereira T.M., Lechner M.C. Arch. Biochem. Biophys. 298:715-725(1992) [PubMed: 1417000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-36, INDUCTION. Tissue: Liver.
[6]	"Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms." Nagata K., Gonzalez F.J., Yamazoe Y., Kato R. J. Biochem. 107:718-725(1990) [PubMed: 2398038] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-33, TISSUE SPECIFICITY, INDUCTION. Tissue: Liver.
[7]	"Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats." Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E. Arch. Biochem. Biophys. 301:345-354(1993) [PubMed: 7681660] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M13646 mRNA. Translation: AAA41051.1. X79319 Genomic DNA. Translation: CAA55887.1. X79320 mRNA. Translation: CAA55888.1. U09742 mRNA. Translation: AAA82168.1. U09734 U09733 Genomic DNA. Translation: AAB60492.1. BC089765 mRNA. Translation: AAH89765.1.
IPI	IPI00196764.
PIR	A25222.
RefSeq	NP_695224.2. NM_153312.2.
UniGene	Rn.225885.
3D structure databases
ProteinModelPortal	P05183.
SMR	P05183. Positions 30-497.
ModBase	Search...
Protein-protein interaction databases
STRING	P05183.
Proteomic databases
PRIDE	P05183.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	266682.
KEGG	rno:266682.
UCSC	NM_153312. rat.
Organism-specific databases
CTD	266682.
RGD	708379. Cyp3a2.
Phylogenomic databases
eggNOG	maNOG12931.
GeneTree	ENSGT00540000069787.
HOVERGEN	HBG108567.
PhylomeDB	P05183.
Gene expression databases
Genevestigator	P05183.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR008072. Cyt_P450_E_CYP3A. IPR002402. Cyt_P450_E_grp-II. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
KO	K07424.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00464. EP450II. PR01689. EP450IICYP3A. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	624480.

Entry information

Entry name

CP3A2_RAT

Accession

Primary (citable) accession number: P05183
Secondary accession number(s): Q5FVU5, Q64629, Q64672

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	July 15, 1999
Last modified:	November 16, 2011
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents