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P05183 (CP3A2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 3A2

EC=1.14.14.1
Alternative name(s):
CYPIIIA2
Cytochrome P450-PCN2
Cytochrome P450/6-beta-A
Testosterone 6-beta-hydroxylase
Gene names
Name:Cyp3a2
Synonyms:Cyp3a-2, Cyp3a11
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Expressed in liver. Ref.6 Ref.7

Induction

By pregnenolone 16-alpha-carbonitrile (PNCN) and dexamethasone. Ref.5 Ref.6 Ref.7

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Cytochrome P450 3A2
PRO_0000051784

Sites

Metal binding4431Iron (heme axial ligand)

Experimental info

Sequence conflict56 – 572GL → AV in CAA55887. Ref.2
Sequence conflict4291D → H in AAA41051. Ref.1
Sequence conflict4451G → D in AAA41051. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P05183 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: C2C84AF736035AB2

FASTA50457,732
        10         20         30         40         50         60 
MDLLSALTLE TWVLLAVILV LLYRLGTHRH GIFKKQGIPG PKPLPFLGTV LNYYKGLGRF 

        70         80         90        100        110        120 
DMECYKKYGK IWGLFDGQTP VFAIMDTEMI KNVLVKECFS VFTNRRDFGP VGIMGKAVSV 

       130        140        150        160        170        180 
AKDEEWKRYR ALLSPTFTSG RLKEMFPIIE QYGDILVKYL KQEAETGKPV TMKKVFGAYS 

       190        200        210        220        230        240 
MDVITSTSFG VNVDSLNNPK DPFVEKTKKL LRFDFFDPLF LSVVLFPFLT PIYEMLNICM 

       250        260        270        280        290        300 
FPKDSIAFFQ KFVHRIKETR LDSKHKHRVD FLQLMLNAHN NSKDEVSHKA LSDVEIIAQS 

       310        320        330        340        350        360 
VIFIFAGYET TSSTLSFVLY FLATHPDIQK KLQEEIDGAL PSKAPPTYDI VMEMEYLDMV 

       370        380        390        400        410        420 
LNETLRLYPI GNRLERVCKK DIELDGLFIP KGSVVTIPTY ALHHDPQHWP KPEEFHPERF 

       430        440        450        460        470        480 
SKENKGSIDP YVYLPFGNGP RNCIGMRFAL MNMKLALTKV LQNFSFQPCK ETQIPLKLSR 

       490        500 
QAILEPEKPI VLKVLPRDAV INGA 

« Hide

References

« Hide 'large scale' references
[1]"Pregnenolone 16 alpha-carbonitrile-inducible P-450 gene family: gene conversion and differential regulation."
Gonzalez F.J., Song B.-J., Hardwick J.P.
Mol. Cell. Biol. 6:2969-2976(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A gene structure of testosterone 6 beta-hydroxylase (P450IIIA)."
Miyata M., Nagata K., Yamazoe Y., Kato R.
Biochem. Biophys. Res. Commun. 177:68-73(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[3]"Structure of a gene and cDNA of a major constitutive form of testosterone 6 beta-hydroxylase (P450/6 beta A) encoding CYP3A2: comparison of the cDNA with P450PCN2."
Miyata M., Nagata K., Shimada M., Yamazoe Y., Kato R.
Arch. Biochem. Biophys. 314:351-359(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[5]"Effect of dexamethasone and phenobarbital on run-on transcription rate and CYP3A mRNA concentration in rat liver: changes during development."
Telhada M.B., Pereira T.M., Lechner M.C.
Arch. Biochem. Biophys. 298:715-725(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-36, INDUCTION.
Tissue: Liver.
[6]"Purification and characterization of four catalytically active testosterone 6 beta-hydroxylase P-450s from rat liver microsomes: comparison of a novel form with three structurally and functionally related forms."
Nagata K., Gonzalez F.J., Yamazoe Y., Kato R.
J. Biochem. 107:718-725(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-33, TISSUE SPECIFICITY, INDUCTION.
Tissue: Liver.
[7]"Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats."
Cooper K.O., Reik L.M., Jayyosi Z., Bandiera S., Kelley M., Ryan D.E., Daniel R., McCluskey S.A., Levin W., Thomas P.E.
Arch. Biochem. Biophys. 301:345-354(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-25, TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13646 mRNA. Translation: AAA41051.1.
X79319 Genomic DNA. Translation: CAA55887.1.
X79320 mRNA. Translation: CAA55888.1.
U09742 mRNA. Translation: AAA82168.1.
U09734 expand/collapse EMBL AC list , U09725, U09726, U09727, U09728, U09729, U09730, U09731, U09732, U09733 Genomic DNA. Translation: AAB60492.1.
BC089765 mRNA. Translation: AAH89765.1.
PIRA25222.
RefSeqNP_695224.2. NM_153312.2.
UniGeneRn.225885.

3D structure databases

ProteinModelPortalP05183.
SMRP05183. Positions 30-497.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP05183.
ChEMBLCHEMBL3324.

Proteomic databases

PaxDbP05183.
PRIDEP05183.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID266682.
KEGGrno:266682.

Organism-specific databases

CTD266682.
RGD708379. Cyp3a2.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000039127.
HOVERGENHBG108567.
KOK07424.

Enzyme and pathway databases

SABIO-RKP05183.

Gene expression databases

GenevestigatorP05183.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR008072. Cyt_P450_E_CYP3A.
IPR002402. Cyt_P450_E_grp-II.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00464. EP450II.
PR01689. EP450IICYP3A.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio624480.

Entry information

Entry nameCP3A2_RAT
AccessionPrimary (citable) accession number: P05183
Secondary accession number(s): Q5FVU5, Q64629, Q64672
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 15, 1999
Last modified: January 22, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families