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P05182

- CP2E1_RAT

UniProt

P05182 - CP2E1_RAT

Protein

Cytochrome P450 2E1

Gene

Cyp2e1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites.By similarity

    Catalytic activityi

    4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi437 – 4371Iron (heme axial ligand)

    GO - Molecular functioni

    1. FMN binding Source: RGD
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro
    4. monooxygenase activity Source: RGD
    5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB-EC
    6. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: RGD

    GO - Biological processi

    1. drug metabolic process Source: Ensembl
    2. heterocycle metabolic process Source: Ensembl
    3. monoterpenoid metabolic process Source: Ensembl
    4. oxidation-reduction process Source: RGD
    5. response to drug Source: RGD
    6. response to ethanol Source: RGD
    7. response to organonitrogen compound Source: RGD
    8. response to ozone Source: RGD
    9. steroid metabolic process Source: Ensembl
    10. triglyceride metabolic process Source: RGD
    11. xenobiotic metabolic process Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2E1 (EC:1.14.13.-)
    Alternative name(s):
    4-nitrophenol 2-hydroxylase (EC:1.14.13.n7)
    CYPIIE1
    Cytochrome P450-J
    Cytochrome P450RLM6
    Gene namesi
    Name:Cyp2e1
    Synonyms:Cyp2e, Cyp2e-1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2475. Cyp2e1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: RGD
    2. intracellular membrane-bounded organelle Source: RGD
    3. intrinsic component of endoplasmic reticulum membrane Source: RGD
    4. mitochondrion Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 493492Cytochrome P450 2E1PRO_0000051758Add
    BLAST

    Proteomic databases

    PaxDbiP05182.
    PRIDEiP05182.

    PTM databases

    PhosphoSiteiP05182.

    Expressioni

    Inductioni

    By ethanol.

    Gene expression databases

    GenevestigatoriP05182.

    Interactioni

    Protein-protein interaction databases

    BioGridi247159. 2 interactions.
    IntActiP05182. 1 interaction.
    MINTiMINT-4576828.
    STRINGi10116.ENSRNOP00000016883.

    Structurei

    3D structure databases

    ProteinModelPortaliP05182.
    SMRiP05182. Positions 33-493.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni298 – 3036Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00680000099783.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    InParanoidiP05182.
    KOiK07415.
    OMAiPMYTMEN.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP05182.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01687. EP450ICYP2E.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05182-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVLGITIAL LVWVATLLVI SIWKQIYNSW NLPPGPFPLP ILGNIFQLDL    50
    KDIPKSFTKL AKRFGPVFTL HLGSRRIVVL HGYKAVKEVL LNHKNEFSGR 100
    GDIPVFQEYK NKGIIFNNGP TWKDVRRFSL SILRDWGMGK QGNEARIQRE 150
    AQFLVEELKK TKGQPFDPTF LIGCAPCNVI ADILFNKRFD YNDKKCLRLM 200
    SLFNENFYLL STPWIQLYNN FADYLRYLPG SHRKIMKNVS EIKQYTLEKA 250
    KEHLQSLDIN CARDVTDCLL IEMEKEKHSQ EPMYTMENVS VTLADLFFAG 300
    TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRVPA VRDRLDMPYM 350
    DAVVHEIQRF INLVPSNLPH EATRDTVFQG YVIPKGTVVI PTLDSLLYDS 400
    HEFPDPEKFK PEHFLNENGK FKYSDYFKAF SAGKRVCVGE GLARMELFLL 450
    LSAILQHFNL KSLVDPKDID LSPVTVGFGS IPPQFKLCVI PRS 493
    Length:493
    Mass (Da):56,627
    Last modified:January 23, 2007 - v4
    Checksum:i7600B9D28AED5EDB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti25 – 251Q → K in AAA41060. (PubMed:3782137)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02627 mRNA. Translation: AAA41060.1.
    M20131 Genomic DNA. Translation: AAA41033.1.
    AF061442 mRNA. Translation: AAC15991.1.
    BC081774 mRNA. Translation: AAH81774.1.
    S48325 mRNA. Translation: AAB24151.1.
    PIRiA25341.
    RefSeqiNP_113731.1. NM_031543.1.
    UniGeneiRn.1372.

    Genome annotation databases

    EnsembliENSRNOT00000016883; ENSRNOP00000016883; ENSRNOG00000012458.
    GeneIDi25086.
    KEGGirno:25086.
    UCSCiRGD:2475. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02627 mRNA. Translation: AAA41060.1 .
    M20131 Genomic DNA. Translation: AAA41033.1 .
    AF061442 mRNA. Translation: AAC15991.1 .
    BC081774 mRNA. Translation: AAH81774.1 .
    S48325 mRNA. Translation: AAB24151.1 .
    PIRi A25341.
    RefSeqi NP_113731.1. NM_031543.1.
    UniGenei Rn.1372.

    3D structure databases

    ProteinModelPortali P05182.
    SMRi P05182. Positions 33-493.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 247159. 2 interactions.
    IntActi P05182. 1 interaction.
    MINTi MINT-4576828.
    STRINGi 10116.ENSRNOP00000016883.

    Chemistry

    BindingDBi P05182.
    ChEMBLi CHEMBL5978.

    PTM databases

    PhosphoSitei P05182.

    Proteomic databases

    PaxDbi P05182.
    PRIDEi P05182.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000016883 ; ENSRNOP00000016883 ; ENSRNOG00000012458 .
    GeneIDi 25086.
    KEGGi rno:25086.
    UCSCi RGD:2475. rat.

    Organism-specific databases

    CTDi 1571.
    RGDi 2475. Cyp2e1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00680000099783.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    InParanoidi P05182.
    KOi K07415.
    OMAi PMYTMEN.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P05182.
    TreeFami TF352043.

    Miscellaneous databases

    NextBioi 605350.
    PROi P05182.

    Gene expression databases

    Genevestigatori P05182.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01687. EP450ICYP2E.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s. Transcriptional and post-transcriptional regulation of the rat enzyme."
      Song B.-J., Gelboin H.V., Park S.-S., Yang C.S., Gonzalez F.J.
      J. Biol. Chem. 261:16689-16697(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The rat P450IIE1 gene: complete intron and exon sequence, chromosome mapping, and correlation of developmental expression with specific 5' cytosine demethylation."
      Umeno M., Song B.-J., Kozak C., Gelboin H.V., Gonzalez F.J.
      J. Biol. Chem. 263:4956-4962(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Liver.
    3. "The complete coding sequence of the rat brain cytochrome P450 2E1."
      Yoo M., Shin S.W.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: Sprague-Dawley.
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    5. "Responses to insulin by two forms of rat hepatic microsomal cytochrome P-450 that undergo major (RLM6) and minor (RLM5b) elevations in diabetes."
      Favreau L.V., Malchoff D.M., Mole J.E., Schenkman J.B.
      J. Biol. Chem. 262:14319-14326(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-35.
      Tissue: Liver.
    6. "Changes in the amount of cytochrome P450s in rat hepatic microsomes with starvation."
      Imaoka S., Terano Y., Funae Y.
      Arch. Biochem. Biophys. 278:168-178(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-20.
      Tissue: Liver.
    7. "Molecular cloning of a cDNA for rat diabetes-inducible cytochrome P450RLM6: hormonal regulation and similarity to the cytochrome P4502E1 gene."
      Richardson T.H., Schenkman J.B., Turcan R., Goldfarb P.S., Gibson G.G.
      Xenobiotica 22:621-631(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 100-463.

    Entry informationi

    Entry nameiCP2E1_RAT
    AccessioniPrimary (citable) accession number: P05182
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 137 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3