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P05182

- CP2E1_RAT

UniProt

P05182 - CP2E1_RAT

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Protein

Cytochrome P450 2E1

Gene

Cyp2e1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites.By similarity

Catalytic activityi

4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi437 – 4371Iron (heme axial ligand)

GO - Molecular functioni

  1. FMN binding Source: RGD
  2. heme binding Source: UniProtKB
  3. iron ion binding Source: InterPro
  4. monooxygenase activity Source: RGD
  5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB-EC
  6. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: RGD

GO - Biological processi

  1. drug metabolic process Source: Ensembl
  2. heterocycle metabolic process Source: Ensembl
  3. monoterpenoid metabolic process Source: Ensembl
  4. oxidation-reduction process Source: RGD
  5. response to drug Source: RGD
  6. response to ethanol Source: RGD
  7. response to organonitrogen compound Source: RGD
  8. response to ozone Source: RGD
  9. steroid metabolic process Source: Ensembl
  10. triglyceride metabolic process Source: RGD
  11. xenobiotic metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2E1 (EC:1.14.13.-)
Alternative name(s):
4-nitrophenol 2-hydroxylase (EC:1.14.13.n7)
CYPIIE1
Cytochrome P450-J
Cytochrome P450RLM6
Gene namesi
Name:Cyp2e1
Synonyms:Cyp2e, Cyp2e-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi2475. Cyp2e1.

Subcellular locationi

GO - Cellular componenti

  1. Golgi membrane Source: RGD
  2. intracellular membrane-bounded organelle Source: RGD
  3. intrinsic component of endoplasmic reticulum membrane Source: RGD
  4. mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 493492Cytochrome P450 2E1PRO_0000051758Add
BLAST

Proteomic databases

PaxDbiP05182.
PRIDEiP05182.

PTM databases

PhosphoSiteiP05182.

Expressioni

Inductioni

By ethanol.

Gene expression databases

ExpressionAtlasiP05182. baseline.
GenevestigatoriP05182.

Interactioni

Protein-protein interaction databases

BioGridi247159. 2 interactions.
IntActiP05182. 1 interaction.
MINTiMINT-4576828.
STRINGi10116.ENSRNOP00000016883.

Structurei

3D structure databases

ProteinModelPortaliP05182.
SMRiP05182. Positions 33-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni298 – 3036Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP05182.
KOiK07415.
OMAiPMYTMEN.
OrthoDBiEOG7RBZ85.
PhylomeDBiP05182.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01687. EP450ICYP2E.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05182-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVLGITIAL LVWVATLLVI SIWKQIYNSW NLPPGPFPLP ILGNIFQLDL
60 70 80 90 100
KDIPKSFTKL AKRFGPVFTL HLGSRRIVVL HGYKAVKEVL LNHKNEFSGR
110 120 130 140 150
GDIPVFQEYK NKGIIFNNGP TWKDVRRFSL SILRDWGMGK QGNEARIQRE
160 170 180 190 200
AQFLVEELKK TKGQPFDPTF LIGCAPCNVI ADILFNKRFD YNDKKCLRLM
210 220 230 240 250
SLFNENFYLL STPWIQLYNN FADYLRYLPG SHRKIMKNVS EIKQYTLEKA
260 270 280 290 300
KEHLQSLDIN CARDVTDCLL IEMEKEKHSQ EPMYTMENVS VTLADLFFAG
310 320 330 340 350
TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRVPA VRDRLDMPYM
360 370 380 390 400
DAVVHEIQRF INLVPSNLPH EATRDTVFQG YVIPKGTVVI PTLDSLLYDS
410 420 430 440 450
HEFPDPEKFK PEHFLNENGK FKYSDYFKAF SAGKRVCVGE GLARMELFLL
460 470 480 490
LSAILQHFNL KSLVDPKDID LSPVTVGFGS IPPQFKLCVI PRS
Length:493
Mass (Da):56,627
Last modified:January 23, 2007 - v4
Checksum:i7600B9D28AED5EDB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251Q → K in AAA41060. (PubMed:3782137)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02627 mRNA. Translation: AAA41060.1.
M20131 Genomic DNA. Translation: AAA41033.1.
AF061442 mRNA. Translation: AAC15991.1.
BC081774 mRNA. Translation: AAH81774.1.
S48325 mRNA. Translation: AAB24151.1.
PIRiA25341.
RefSeqiNP_113731.1. NM_031543.1.
UniGeneiRn.1372.

Genome annotation databases

EnsembliENSRNOT00000016883; ENSRNOP00000016883; ENSRNOG00000012458.
GeneIDi25086.
KEGGirno:25086.
UCSCiRGD:2475. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02627 mRNA. Translation: AAA41060.1 .
M20131 Genomic DNA. Translation: AAA41033.1 .
AF061442 mRNA. Translation: AAC15991.1 .
BC081774 mRNA. Translation: AAH81774.1 .
S48325 mRNA. Translation: AAB24151.1 .
PIRi A25341.
RefSeqi NP_113731.1. NM_031543.1.
UniGenei Rn.1372.

3D structure databases

ProteinModelPortali P05182.
SMRi P05182. Positions 33-493.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 247159. 2 interactions.
IntActi P05182. 1 interaction.
MINTi MINT-4576828.
STRINGi 10116.ENSRNOP00000016883.

Chemistry

BindingDBi P05182.
ChEMBLi CHEMBL5978.

PTM databases

PhosphoSitei P05182.

Proteomic databases

PaxDbi P05182.
PRIDEi P05182.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000016883 ; ENSRNOP00000016883 ; ENSRNOG00000012458 .
GeneIDi 25086.
KEGGi rno:25086.
UCSCi RGD:2475. rat.

Organism-specific databases

CTDi 1571.
RGDi 2475. Cyp2e1.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00760000118775.
HOGENOMi HOG000036992.
HOVERGENi HBG015789.
InParanoidi P05182.
KOi K07415.
OMAi PMYTMEN.
OrthoDBi EOG7RBZ85.
PhylomeDBi P05182.
TreeFami TF352043.

Miscellaneous databases

NextBioi 605350.
PROi P05182.

Gene expression databases

ExpressionAtlasi P05182. baseline.
Genevestigatori P05182.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01687. EP450ICYP2E.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s. Transcriptional and post-transcriptional regulation of the rat enzyme."
    Song B.-J., Gelboin H.V., Park S.-S., Yang C.S., Gonzalez F.J.
    J. Biol. Chem. 261:16689-16697(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The rat P450IIE1 gene: complete intron and exon sequence, chromosome mapping, and correlation of developmental expression with specific 5' cytosine demethylation."
    Umeno M., Song B.-J., Kozak C., Gelboin H.V., Gonzalez F.J.
    J. Biol. Chem. 263:4956-4962(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  3. "The complete coding sequence of the rat brain cytochrome P450 2E1."
    Yoo M., Shin S.W.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  5. "Responses to insulin by two forms of rat hepatic microsomal cytochrome P-450 that undergo major (RLM6) and minor (RLM5b) elevations in diabetes."
    Favreau L.V., Malchoff D.M., Mole J.E., Schenkman J.B.
    J. Biol. Chem. 262:14319-14326(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-35.
    Tissue: Liver.
  6. "Changes in the amount of cytochrome P450s in rat hepatic microsomes with starvation."
    Imaoka S., Terano Y., Funae Y.
    Arch. Biochem. Biophys. 278:168-178(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20.
    Tissue: Liver.
  7. "Molecular cloning of a cDNA for rat diabetes-inducible cytochrome P450RLM6: hormonal regulation and similarity to the cytochrome P4502E1 gene."
    Richardson T.H., Schenkman J.B., Turcan R., Goldfarb P.S., Gibson G.G.
    Xenobiotica 22:621-631(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 100-463.

Entry informationi

Entry nameiCP2E1_RAT
AccessioniPrimary (citable) accession number: P05182
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 138 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3