P05182 (CP2E1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2E1
EC=1.14.13.-

Alternative name(s):
4-nitrophenol 2-hydroxylase
EC=1.14.13.n7
CYPIIE1
Cytochrome P450-J
Cytochrome P450RLM6

Gene names

Name:	Cyp2e1
Synonyms:	Cyp2e, Cyp2e-1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites By similarity.
Catalytic activity	4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By ethanol.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	drug metabolic process Inferred from electronic annotation. Source: Compara heterocycle metabolic process Inferred from electronic annotation. Source: Compara monoterpenoid metabolic process Inferred from electronic annotation. Source: Compara response to drug Inferred from expression pattern PubMed 21170329. Source: RGD response to ethanol Inferred from expression pattern PubMed 16337197. Source: RGD response to organic nitrogen Inferred from expression pattern PubMed 19406192. Source: RGD response to ozone Inferred from expression pattern PubMed 9571988. Source: RGD steroid metabolic process Inferred from electronic annotation. Source: Compara triglyceride metabolic process Inferred from mutant phenotype PubMed 20116195. Source: RGD xenobiotic metabolic process Inferred from mutant phenotype PubMed 21187827. Source: RGD
Cellular_component	Golgi membrane Inferred from direct assay PubMed 8809087. Source: RGD intrinsic to endoplasmic reticulum membrane Inferred from direct assay PubMed 14661969. Source: RGD mitochondrion Inferred from direct assay PubMed 16337197. Source: RGD
Molecular_function	FMN binding Inferred by curator PubMed 8809087. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from sequence or structural similarity. Source: UniProtKB iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Inferred from direct assay PubMed 8809087. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5 Ref.6
	Chain	2 – 493	492	Cytochrome P450 2E1		PRO_0000051758
Regions
	Region	298 – 303	6	Substrate binding By similarity
Sites
	Metal binding	437	1	Iron (heme axial ligand)
Amino acid modifications
	Modified residue	129	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	25	1	Q → K in AAA41060. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P05182 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 7600B9D28AED5EDB	FASTA	493	56,627
	10 20 30 40 50 60 MAVLGITIAL LVWVATLLVI SIWKQIYNSW NLPPGPFPLP ILGNIFQLDL KDIPKSFTKL 70 80 90 100 110 120 AKRFGPVFTL HLGSRRIVVL HGYKAVKEVL LNHKNEFSGR GDIPVFQEYK NKGIIFNNGP 130 140 150 160 170 180 TWKDVRRFSL SILRDWGMGK QGNEARIQRE AQFLVEELKK TKGQPFDPTF LIGCAPCNVI 190 200 210 220 230 240 ADILFNKRFD YNDKKCLRLM SLFNENFYLL STPWIQLYNN FADYLRYLPG SHRKIMKNVS 250 260 270 280 290 300 EIKQYTLEKA KEHLQSLDIN CARDVTDCLL IEMEKEKHSQ EPMYTMENVS VTLADLFFAG 310 320 330 340 350 360 TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRVPA VRDRLDMPYM DAVVHEIQRF 370 380 390 400 410 420 INLVPSNLPH EATRDTVFQG YVIPKGTVVI PTLDSLLYDS HEFPDPEKFK PEHFLNENGK 430 440 450 460 470 480 FKYSDYFKAF SAGKRVCVGE GLARMELFLL LSAILQHFNL KSLVDPKDID LSPVTVGFGS 490 IPPQFKLCVI PRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s. Transcriptional and post-transcriptional regulation of the rat enzyme." Song B.-J., Gelboin H.V., Park S.-S., Yang C.S., Gonzalez F.J. J. Biol. Chem. 261:16689-16697(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The rat P450IIE1 gene: complete intron and exon sequence, chromosome mapping, and correlation of developmental expression with specific 5' cytosine demethylation." Umeno M., Song B.-J., Kozak C., Gelboin H.V., Gonzalez F.J. J. Biol. Chem. 263:4956-4962(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[3]	"The complete coding sequence of the rat brain cytochrome P450 2E1." Yoo M., Shin S.W. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Sprague-Dawley. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[5]	"Responses to insulin by two forms of rat hepatic microsomal cytochrome P-450 that undergo major (RLM6) and minor (RLM5b) elevations in diabetes." Favreau L.V., Malchoff D.M., Mole J.E., Schenkman J.B. J. Biol. Chem. 262:14319-14326(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-35. Tissue: Liver.
[6]	"Changes in the amount of cytochrome P450s in rat hepatic microsomes with starvation." Imaoka S., Terano Y., Funae Y. Arch. Biochem. Biophys. 278:168-178(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20. Tissue: Liver.
[7]	"Molecular cloning of a cDNA for rat diabetes-inducible cytochrome P450RLM6: hormonal regulation and similarity to the cytochrome P4502E1 gene." Richardson T.H., Schenkman J.B., Turcan R., Goldfarb P.S., Gibson G.G. Xenobiotica 22:621-631(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 100-463.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02627 mRNA. Translation: AAA41060.1. M20131 Genomic DNA. Translation: AAA41033.1. AF061442 mRNA. Translation: AAC15991.1. BC081774 mRNA. Translation: AAH81774.1. S48325 mRNA. Translation: AAB24151.1.
IPI	IPI00324912.
PIR	A25341.
RefSeq	NP_113731.1. NM_031543.1.
UniGene	Rn.1372.
3D structure databases
ProteinModelPortal	P05182.
SMR	P05182. Positions 33-493.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000016883.
PTM databases
PhosphoSite	P05182.
Proteomic databases
PaxDb	P05182.
PRIDE	P05182.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016883; ENSRNOP00000016883; ENSRNOG00000012458.
GeneID	25086.
KEGG	rno:25086.
UCSC	RGD:2475. rat.
Organism-specific databases
CTD	1571.
RGD	2475. Cyp2e1.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00680000099783.
HOGENOM	HOG000036992.
HOVERGEN	HBG015789.
InParanoid	P05182.
KO	K07415.
OMA	PMYTMEN.
OrthoDB	EOG48WC22.
Gene expression databases
ArrayExpress	P05182.
Genevestigator	P05182.
GermOnline	ENSRNOG00000012458. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008070. Cyt_P450_E_grp-I_CYP2E-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01687. EP450ICYP2E. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P05182.
ChEMBL	CHEMBL5978.
NextBio	605350.

Entry information

Entry name

CP2E1_RAT

Accession

Primary (citable) accession number: P05182

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 127 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program