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P05179 (CP2C7_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2C7

EC=1.14.14.1
Alternative name(s):
CYPIIC7
Cytochrome P450F
PTF1
Gene names
Name:Cyp2c7
Synonyms:Cyp2c-7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

By growth hormone. P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens. Ref.3

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Cytochrome P450 2C7
PRO_0000051698

Sites

Metal binding4351Iron (heme axial ligand)

Amino acid modifications

Modified residue1441Omega-N-methylated arginine Ref.7

Experimental info

Sequence conflict281K → E in AAA41058. Ref.5
Sequence conflict57 – 659FSKTYGPVF → VSIVGDPVI in CAA31108. Ref.2
Sequence conflict871L → V no nucleotide entry Ref.6
Sequence conflict1031N → I in AAA41058. Ref.5
Sequence conflict1031N → I no nucleotide entry Ref.6
Sequence conflict1301N → T no nucleotide entry Ref.6
Sequence conflict2791Q → H in AAA41058. Ref.5
Sequence conflict351 – 3522MI → HD in AAA41058. Ref.5
Sequence conflict383 – 3853KGT → RRA no nucleotide entry Ref.6
Sequence conflict408 – 4114DPGH → VPWP no nucleotide entry Ref.6
Sequence conflict4711P → A no nucleotide entry Ref.6

Sequences

Sequence LengthMass (Da)Tools
P05179 [UniParc].

Last modified October 1, 1989. Version 2.
Checksum: 0FBACF3E9C6ABAA5

FASTA49056,187
        10         20         30         40         50         60 
MDLVTFLVLT LSSLILLSLW RQSSRRRKLP PGPTPLPIIG NFLQIDVKNI SQSLTKFSKT 

        70         80         90        100        110        120 
YGPVFTLYLG SQPTVILHGY EAIKEALIDN GEKFSGRGSY PMNENVTKGF GIVFSNGNRW 

       130        140        150        160        170        180 
KEMRRFTIMN FRNLGIGKRN IEDRVQEEAQ CLVEELRKTK GSPCDPSLIL NCAPCNVICS 

       190        200        210        220        230        240 
ITFQNHFDYK DKEMLTFMEK VNENLKIMSS PWMQVCNSFP SLIDYFPGTH HKIAKNINYM 

       250        260        270        280        290        300 
KSYLLKKIEE HQESLDVTNP RDFVDYYLIK QKQANNIEQS EYSHENLTCS IMDLIGAGTE 

       310        320        330        340        350        360 
TMSTTLRYAL LLLMKYPHVT AKVQEEIDRV IGRHRSPCMQ DRKHMPYTDA MIHEVQRFIN 

       370        380        390        400        410        420 
FVPTNLPHAV TCDIKFRNYL IPKGTKVLTS LTSVLHDSKE FPNPEMFDPG HFLDENGNFK 

       430        440        450        460        470        480 
KSDYFLPFSA GKRACVGEGL ARMQLFLFLT TILQNFNLKS LVHPKDIDTM PVLNGFASLP 

       490 
PTYQLCFIPS 

« Hide

References

[1]"Complementary DNA cloning of cytochrome P-450s related to P-450(M-1) from the complementary DNA library of female rat livers. Predicted primary structures for P-450f, PB-1, and PB-1-related protein with a bizarre replacement block and their mode of transcriptional expression."
Kimura H., Yoshioka H., Sogawa K., Sakai Y., Fujii-Kuriyama Y.
J. Biol. Chem. 263:701-707(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"5' flanking sequence of the gene for rat cytochrome p-450f."
Stroem A., Nilsson A.G., Zaphiropoulos P.G.
Submitted (AUG-1988) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-66.
Strain: Sprague-Dawley.
[3]"Growth hormone regulation of the cytochrome P-450IIC subfamily in the rat: inductive, repressive, and transcriptional effects on P-450f (IIC7) and P-450PB1 (IIC6) gene expression."
Westin S., Stroem A., Gustafsson J.-A., Zaphiropoulos P.G.
Mol. Pharmacol. 38:192-197(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-56, INDUCTION.
[4]"Responses to insulin by two forms of rat hepatic microsomal cytochrome P-450 that undergo major (RLM6) and minor (RLM5b) elevations in diabetes."
Favreau L.V., Malchoff D.M., Mole J.E., Schenkman J.B.
J. Biol. Chem. 262:14319-14326(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-23.
Tissue: Liver.
[5]"Sequence of two related P-450 mRNAs transcriptionally increased during rat development. An R.dre.1 sequence occupies the complete 3' untranslated region of a liver mRNA."
Gonzalez F.J., Kimura S., Song B.-J., Pastewka J., Gelboin H.V., Hardwick J.P.
J. Biol. Chem. 261:10667-10672(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-490.
[6]"Isolation and characterization of cDNA clones for cytochromes P-450 immunochemically related to rat hepatic P-450 form PB-1."
Friedberg T., Waxman D.J., Atchison M., Kumar A., Haaparanta T., Raphael C., Adesnik M.
Biochemistry 25:7975-7983(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 87-490.
[7]"Organellar proteomics reveals Golgi arginine dimethylation."
Wu C.C., MacCoss M.J., Mardones G., Finnigan C., Mogelsvang S., Yates J.R. III, Howell K.E.
Mol. Biol. Cell 15:2907-2919(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT ARG-144, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M18335 mRNA. Translation: AAA41036.1.
X12595 Genomic DNA. Translation: CAA31108.1.
M31031 mRNA. Translation: AAA41058.1.
PIRB28516.
RefSeqNP_058854.1. NM_017158.2.
UniGeneRn.1247.

3D structure databases

ProteinModelPortalP05179.
SMRP05179. Positions 30-489.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP05179. 1 interaction.
MINTMINT-4565026.

PTM databases

PhosphoSiteP05179.

Proteomic databases

PRIDEP05179.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29298.
KEGGrno:29298.

Organism-specific databases

CTD29298.
RGD620379. Cyp2c7.

Phylogenomic databases

HOVERGENHBG015789.
InParanoidP05179.
KOK07413.
PhylomeDBP05179.

Gene expression databases

GenevestigatorP05179.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608708.

Entry information

Entry nameCP2C7_RAT
AccessionPrimary (citable) accession number: P05179
Secondary accession number(s): Q63706
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: October 1, 1989
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families