Cytochrome P450 2C7 - Rattus norvegicus (Rat)

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P05179 (CP2C7_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 2C7
EC=1.14.14.1

Alternative name(s):
CYPIIC7
Cytochrome P450F
PTF1

Gene names

Name:	Cyp2c7
Synonyms:	Cyp2c-7

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By growth hormone. P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens. Ref.3
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Methylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	response to drug Inferred from expression pattern PubMed 15634872. Source: RGD response to ethanol Inferred from expression pattern PubMed 10660124. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 8435092. Source: RGD response to nutrient Inferred from expression pattern PubMed 8534269. Source: RGD response to organic cyclic compound Inferred from expression pattern PubMed 8471063. Source: RGD response to peptide hormone stimulus Inferred from expression pattern PubMed 10660124. Source: RGD response to retinoic acid Inferred from expression pattern PubMed 9202400. Source: RGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular membrane-bounded organelle Inferred from direct assay PubMed 15363580. Source: RGD
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 490

490

Cytochrome P450 2C7

PRO_0000051698

Sites

Metal binding

435

Iron (heme axial ligand)

Amino acid modifications

Modified residue

144

Omega-N-methylated arginine Ref.7

Experimental info

Sequence conflict

K → E in AAA41058. Ref.5

Sequence conflict

57 – 65

FSKTYGPVF → VSIVGDPVI in CAA31108. Ref.2

Sequence conflict

L → V no nucleotide entry Ref.6

Sequence conflict

103

N → I in AAA41058. Ref.5

Sequence conflict

103

N → I no nucleotide entry Ref.6

Sequence conflict

130

N → T no nucleotide entry Ref.6

Sequence conflict

279

Q → H in AAA41058. Ref.5

Sequence conflict

351 – 352

MI → HD in AAA41058. Ref.5

Sequence conflict

383 – 385

KGT → RRA no nucleotide entry Ref.6

Sequence conflict

408 – 411

DPGH → VPWP no nucleotide entry Ref.6

Sequence conflict

471

P → A no nucleotide entry Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P05179 [UniParc].

Last modified October 1, 1989. Version 2.
Checksum: 0FBACF3E9C6ABAA5
Show »

FASTA

490

56,187

        10         20         30         40         50         60 
MDLVTFLVLT LSSLILLSLW RQSSRRRKLP PGPTPLPIIG NFLQIDVKNI SQSLTKFSKT 

        70         80         90        100        110        120 
YGPVFTLYLG SQPTVILHGY EAIKEALIDN GEKFSGRGSY PMNENVTKGF GIVFSNGNRW 

       130        140        150        160        170        180 
KEMRRFTIMN FRNLGIGKRN IEDRVQEEAQ CLVEELRKTK GSPCDPSLIL NCAPCNVICS 

       190        200        210        220        230        240 
ITFQNHFDYK DKEMLTFMEK VNENLKIMSS PWMQVCNSFP SLIDYFPGTH HKIAKNINYM 

       250        260        270        280        290        300 
KSYLLKKIEE HQESLDVTNP RDFVDYYLIK QKQANNIEQS EYSHENLTCS IMDLIGAGTE 

       310        320        330        340        350        360 
TMSTTLRYAL LLLMKYPHVT AKVQEEIDRV IGRHRSPCMQ DRKHMPYTDA MIHEVQRFIN 

       370        380        390        400        410        420 
FVPTNLPHAV TCDIKFRNYL IPKGTKVLTS LTSVLHDSKE FPNPEMFDPG HFLDENGNFK 

       430        440        450        460        470        480 
KSDYFLPFSA GKRACVGEGL ARMQLFLFLT TILQNFNLKS LVHPKDIDTM PVLNGFASLP 

       490 
PTYQLCFIPS

« Hide

References

[1]	"Complementary DNA cloning of cytochrome P-450s related to P-450(M-1) from the complementary DNA library of female rat livers. Predicted primary structures for P-450f, PB-1, and PB-1-related protein with a bizarre replacement block and their mode of transcriptional expression." Kimura H., Yoshioka H., Sogawa K., Sakai Y., Fujii-Kuriyama Y. J. Biol. Chem. 263:701-707(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"5' flanking sequence of the gene for rat cytochrome p-450f." Stroem A., Nilsson A.G., Zaphiropoulos P.G. Submitted (AUG-1988) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-66. Strain: Sprague-Dawley.
[3]	"Growth hormone regulation of the cytochrome P-450IIC subfamily in the rat: inductive, repressive, and transcriptional effects on P-450f (IIC7) and P-450PB1 (IIC6) gene expression." Westin S., Stroem A., Gustafsson J.-A., Zaphiropoulos P.G. Mol. Pharmacol. 38:192-197(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-56, INDUCTION.
[4]	"Responses to insulin by two forms of rat hepatic microsomal cytochrome P-450 that undergo major (RLM6) and minor (RLM5b) elevations in diabetes." Favreau L.V., Malchoff D.M., Mole J.E., Schenkman J.B. J. Biol. Chem. 262:14319-14326(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-23. Tissue: Liver.
[5]	"Sequence of two related P-450 mRNAs transcriptionally increased during rat development. An R.dre.1 sequence occupies the complete 3' untranslated region of a liver mRNA." Gonzalez F.J., Kimura S., Song B.-J., Pastewka J., Gelboin H.V., Hardwick J.P. J. Biol. Chem. 261:10667-10672(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 8-490.
[6]	"Isolation and characterization of cDNA clones for cytochromes P-450 immunochemically related to rat hepatic P-450 form PB-1." Friedberg T., Waxman D.J., Atchison M., Kumar A., Haaparanta T., Raphael C., Adesnik M. Biochemistry 25:7975-7983(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 87-490.
[7]	"Organellar proteomics reveals Golgi arginine dimethylation." Wu C.C., MacCoss M.J., Mardones G., Finnigan C., Mogelsvang S., Yates J.R. III, Howell K.E. Mol. Biol. Cell 15:2907-2919(2004) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION AT ARG-144, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M18335 mRNA. Translation: AAA41036.1. X12595 Genomic DNA. Translation: CAA31108.1. M31031 mRNA. Translation: AAA41058.1.
IPI	IPI00196748.
PIR	B28516.
RefSeq	NP_058854.1. NM_017158.2.
UniGene	Rn.1247.
3D structure databases
ProteinModelPortal	P05179.
SMR	P05179. Positions 30-489.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05179. 1 interaction.
PTM databases
PhosphoSite	P05179.
Proteomic databases
PRIDE	P05179.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	29298.
KEGG	rno:29298.
Organism-specific databases
CTD	29298.
RGD	620379. Cyp2c7.
Phylogenomic databases
HOVERGEN	HBG015789.
InParanoid	P05179.
KO	K07413.
Gene expression databases
Genevestigator	P05179.
GermOnline	ENSRNOG00000021405. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	608708.

Entry information

Entry name

CP2C7_RAT

Accession

Primary (citable) accession number: P05179
Secondary accession number(s): Q63706

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents