Reviewed, UniProtKB/Swiss-Prot P05176 (CP1A1_RABIT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 1A1
    EC=1.14.14.1
Alternative name(s):
    CYPIA1
    P450 isozyme 6
    P-450 PHPAH1
    P450 LM6

Gene names

Name:

CYP1A1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and by 3-methylcholanthrene (3MC).
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Cytochrome P450 1A1		PRO_0000051632
Sites
	Metal binding	461	1	Iron (heme axial ligand)
Amino acid modifications
	Modified residue	364	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	69	1	R → L Ref.2
	Sequence conflict	146	1	L → R in AAA31431. Ref.2
	Sequence conflict	176 – 177	2	SK → GR in AAA31431. Ref.2
	Sequence conflict	341 – 345	5	PRIQR → RQHTRE in AAA31431. Ref.2
	Sequence conflict	422	1	P → R in AAA31431. Ref.2
	Sequence conflict	429 – 430	2	EA → RS Ref.2
	Sequence conflict	442 – 443	2	AV → TL Ref.2
	Sequence conflict	446 – 449	4	ALTE → GPDD Ref.2
	Sequence conflict	463	1	G → A in AAA31431. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05176-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: A9D382C5DF122A5A	FASTA	518	58,278
	10 20 30 40 50 60 MVSDFGLPTF ISATELLLAS AVFCLVFWVA GASKPRVPKG LKRLPGPWGW PLLGHVLTLG 70 80 90 100 110 120 KNPHVALARL SRRYGDVFQI RLGSTPVVVL SGLDTIKQAL VRQGDDFKGR PDLYSFSFVT 130 140 150 160 170 180 KGQSMIFGSD SGPVWAARRR LAQNALNSFS VASDPASSSS CYLEEHVSQE AENLISKFQE 190 200 210 220 230 240 LMAAVGHFDP YRYVVMSVAN VICAMCFGRR YDHDDQELLS LVNLNDEFGK VAASGSPADF 250 260 270 280 290 300 FLILRYLPNP ALDTFKDLNE RFYSFTQERV KEHCRSFEKG HIRDITDSLI KHYRVDRLDE 310 320 330 340 350 360 NANVQVSDEK TVGIVLDLFG AGFDTVTTAI SWSLMYLVTK PRIQRKIQEE LDAVVGRARR 370 380 390 400 410 420 PRFSDRPQLP YLEAVIMETF RHTSFLPFTI PHSTTRDTSL GGFYIPKGRC VFVNQWQNNH 430 440 450 460 470 480 DPELWGDPEA FRPERFLTPS GAVDKALTEK VLLFGLGKRK CIGETIGRLE VFLFLATLLQ 490 500 510 QVEFSVSPGT TVDMTPIYGL TMKHARCEHF QAKLRFEA

References

[1]	"Structural analysis of cloned cDNAs for polycyclic hydrocarbon-inducible forms of rabbit liver microsomal cytochrome P-450." Kagawa N., Mihara K., Sato R. J. Biochem. 101:1471-1479(1987) [PubMed: 3667560] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Cloning and characterization of cDNAs encoding 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible rabbit mRNAs for cytochrome P-450 isozymes 4 and 6." Okino S.T., Quattrochi L.C., Barnes H.J., Osanto S., Griffin K.J., Johnson E.F., Tukey R.H. Proc. Natl. Acad. Sci. U.S.A. 82:5310-5314(1985) [PubMed: 2991917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 56-518.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M11727 mRNA. Translation: AAA31431.1. Sequence problems. X05685 mRNA. Translation: CAA29170.1.
PIR	A27821.
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
SMR	P05176. Positions 36-516.
ModBase	Search...
Protein-protein interaction databases
STRING	P05176.
Genome annotation databases
Ensembl	ENSOCUT00000017743; ENSOCUP00000015239; ENSOCUG00000017744; Oryctolagus cuniculus. [Genome view]
Phylogenomic databases
HOVERGEN	P05176.
Enzyme and pathway databases
BRENDA	1.14.14.1. 255.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008066. Cyt_P450_E_grp-I_CYP1. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit. PTHR19383:SF63. Cyt_P450_E_grp-I_CYP1. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01683. EP450ICYP1A. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP1A1_RABIT

Accession

Primary (citable) accession number: P05176

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 1, 1988
Last modified:	October 13, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)