P05167 (ALEU_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Thiol protease aleurain EC=3.4.22.16
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.
Subcellular location	Vacuole. Note: Vacuole-like subcellular compartment.
Induction	Aleurain is synthesized by the aleurone cells stimulated by gibberellic or abscisic acid.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Biological process	Germination
Cellular component	Vacuole
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Propeptide	23 – 143	121	Activation peptide Potential		PRO_0000026416
	Chain	144 – 362	219	Thiol protease aleurain		PRO_0000026417
Sites
	Active site	168	1	By similarity
	Active site	308	1	By similarity
	Active site	328	1	By similarity
Amino acid modifications
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Glycosylation	257	1	N-linked (GlcNAc...) Potential
	Disulfide bond	165 ↔ 208		By similarity
	Disulfide bond	199 ↔ 241		By similarity
	Disulfide bond	299 ↔ 349		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05167 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: A70CCD4A843A1686	FASTA	362	39,122
	10 20 30 40 50 60 MAHARVLLLA LAVLATAAVA VASSSSFADS NPIRPVTDRA ASTLESAVLG ALGRTRHALR 70 80 90 100 110 120 FARFAVRYGK SYESAAEVRR RFRIFSESLE EVRSTNRKGL PYRLGINRFS DMSWEEFQAT 130 140 150 160 170 180 RLGAAQTCSA TLAGNHLMRD AAALPETKDW REDGIVSPVK NQAHCGSCWT FSTTGALEAA 190 200 210 220 230 240 YTQATGKNIS LSEQQLVDCA GGFNNFGCNG GLPSQAFEYI KYNGGIDTEE SYPYKGVNGV 250 260 270 280 290 300 CHYKAENAAV QVLDSVNITL NAEDELKNAV GLVRPVSVAF QVIDGFRQYK SGVYTSDHCG 310 320 330 340 350 360 TTPDDVNHAV LAVGYGVENG VPYWLIKNSW GADWGDNGYF KMEMGKNMCA IATCASYPVV AA

References

[1]	"Aleurain: a barley thiol protease closely related to mammalian cathepsin H." Rogers J.C., Dean D., Heck G.R. Proc. Natl. Acad. Sci. U.S.A. 82:6512-6516(1985) [PubMed: 3901004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Rogers J.C. Submitted (MAR-1987) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05167 Genomic DNA. Translation: CAA28804.1.
PIR	KHBH. A25492.
3D structure databases
ProteinModelPortal	P05167.
SMR	P05167. Positions 145-359.
ModBase	Search...
Protein family/group databases
MEROPS	C01.041.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P05167.
Gene expression databases
Genevestigator	P05167.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALEU_HORVU

Accession

Primary (citable) accession number: P05167

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program