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Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

PCK1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Enzyme regulationi

Activity is affected by a number of hormones regulating this metabolic process (such as glucagon, insulin, or glucocorticoids).

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87SubstrateBy similarity1
Metal bindingi244ManganeseBy similarity1
Metal bindingi264Manganese; via tele nitrogenBy similarity1
Binding sitei286SubstrateBy similarity1
Active sitei288By similarity1
Metal bindingi311ManganeseBy similarity1
Binding sitei405GTPBy similarity1
Binding sitei436GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi287 – 292GTPBy similarity6
Nucleotide bindingi530 – 533GTPBy similarity4

GO - Molecular functioni

  • DBD domain binding Source: AgBase
  • epinephrine binding Source: AgBase
  • GTP binding Source: AgBase
  • inosine kinase activity Source: AgBase
  • malate oxidase activity Source: AgBase
  • manganese ion binding Source: AgBase
  • mRNA binding Source: AgBase
  • nucleoside diphosphate kinase activity Source: AgBase
  • phosphoenolpyruvate carboxykinase (GTP) activity Source: AgBase
  • protein-disulfide reductase activity Source: AgBase
  • sequence-specific DNA binding Source: AgBase

GO - Biological processi

  • adult feeding behavior Source: AgBase
  • aging Source: AgBase
  • alanine metabolic process Source: AgBase
  • aspartate metabolic process Source: AgBase
  • cellular response to cAMP Source: AgBase
  • cellular response to dexamethasone stimulus Source: AgBase
  • cellular response to ethanol Source: AgBase
  • cellular response to fructose stimulus Source: AgBase
  • cellular response to glucagon stimulus Source: AgBase
  • cellular response to glucose starvation Source: AgBase
  • cellular response to glucose stimulus Source: AgBase
  • cellular response to hypoxia Source: AgBase
  • cellular response to insulin stimulus Source: AgBase
  • cellular response to interleukin-1 Source: AgBase
  • cellular response to parathyroid hormone stimulus Source: AgBase
  • cellular response to retinoic acid Source: AgBase
  • cellular response to tumor necrosis factor Source: AgBase
  • developmental growth Source: AgBase
  • digestion Source: AgBase
  • eggshell formation Source: AgBase
  • embryo development ending in birth or egg hatching Source: AgBase
  • embryonic organ morphogenesis Source: AgBase
  • gluconeogenesis Source: AgBase
  • glutamate metabolic process Source: AgBase
  • glutamine metabolic process Source: AgBase
  • glycerol catabolic process Source: AgBase
  • glycine metabolic process Source: AgBase
  • hepatocyte differentiation Source: AgBase
  • intestinal absorption Source: AgBase
  • kidney interstitial fibroblast differentiation Source: AgBase
  • lactate metabolic process Source: AgBase
  • lipid catabolic process Source: AgBase
  • malate transport Source: AgBase
  • NADH regeneration Source: AgBase
  • negative regulation of carbohydrate metabolic process Source: AgBase
  • oviposition Source: AgBase
  • positive regulation of cAMP-mediated signaling Source: AgBase
  • positive regulation of carbohydrate metabolic process Source: AgBase
  • positive regulation of gluconeogenesis Source: AgBase
  • proline metabolic process Source: AgBase
  • propionate catabolic process Source: AgBase
  • pyruvate metabolic process Source: AgBase
  • regulation of feeding behavior Source: AgBase
  • regulation of gluconeogenesis Source: AgBase
  • regulation of transcription by glucose Source: AgBase
  • response to cAMP Source: AgBase
  • response to cycloheximide Source: AgBase
  • response to glucocorticoid Source: AgBase
  • response to interleukin-6 Source: AgBase
  • response to lipid Source: AgBase
  • response to lipopolysaccharide Source: AgBase
  • response to methionine Source: AgBase
  • response to starvation Source: AgBase
  • serine family amino acid metabolic process Source: AgBase
  • sexual reproduction Source: AgBase
  • vitellogenesis Source: AgBase

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-352875. Gluconeogenesis.
SABIO-RKiP05153.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32)
Short name:
PEPCK-C
Gene namesi
Name:PCK1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036311 – 622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]Add BLAST622

Proteomic databases

PaxDbiP05153.

Interactioni

Subunit structurei

Monomer.

GO - Molecular functioni

  • DBD domain binding Source: AgBase

Protein-protein interaction databases

STRINGi9031.ENSGALP00000012342.

Structurei

3D structure databases

ProteinModelPortaliP05153.
SMRiP05153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 237Substrate bindingBy similarity3
Regioni403 – 405Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3749. Eukaryota.
COG1274. LUCA.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiP05153.
KOiK01596.
PhylomeDBiP05153.

Family and domain databases

CDDicd00819. PEPCK_GTP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiView protein in InterPro
IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR035077. PEP_carboxykinase_GTP_C.
IPR035078. PEP_carboxykinase_GTP_N.
IPR008210. PEP_carboxykinase_N.
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiView protein in Pfam
PF00821. PEPCK_C. 1 hit.
PF17297. PEPCK_N. 1 hit.
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiView protein in PROSITE
PS00505. PEPCK_GTP. 1 hit.

Sequencei

Sequence statusi: Complete.

P05153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPELKTEVN IISKVIQGDL ESLPPQVREF IESNAKLCQP ESIHICDGSE
60 70 80 90 100
EENKKILDIM VEQGMIKKLS KYENCWLALT NPRDVARIES KTVIITQEQR
110 120 130 140 150
DTIPIPKTGS SQLGRWMSEE DFEKAFNTRF PGCMQGRTMY VIPFSMGPIG
160 170 180 190 200
SPLAKIGIEL TDSPYVVASM RMMTRMGTAA LKALGNGEFV KCLHSVGCPL
210 220 230 240 250
PLKEPLINNW PCNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRI
260 270 280 290 300
ASRIAKEEGW LAEHMLILGI TNPEGEKKYF AAAFPSACGK TNLAMMNPSR
310 320 330 340 350
PGWKIECVGD DIAWMKFDEL GNLRAINPEN GFFGVAPGTS IKTNPNAIKT
360 370 380 390 400
IFKNTIFTNV AETSDGGVYW EGIDEPLPPG VTLTSWKNKD WTPDNGEPCA
410 420 430 440 450
HPNSRFCTPA SQCPIMDPAW ESPEGVPIEG IIFGGRRPAG VPLVYEAFNW
460 470 480 490 500
QHGVFIGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM
510 520 530 540 550
AHRPAAKLPR IFHVNWFRKD SQGKFLWPGY GENSRVLEWM FNRIQGKASA
560 570 580 590 600
KSTAIGYIPA DTALNLKGLE DINLTELFNI SKEFWEKEVE EIKQYFEGQV
610 620
NADLPYEIER ELLALEMRIK QL
Length:622
Mass (Da):69,528
Last modified:August 13, 1987 - v1
Checksum:i1BB1E708A3D5E722
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14229 mRNA. Translation: AAA49005.1.
PIRiA26494. QYCHGC.
RefSeqiNP_990802.1. NM_205471.1.
UniGeneiGga.4447.

Genome annotation databases

GeneIDi396458.
KEGGigga:396458.

Similar proteinsi

Entry informationi

Entry nameiPCKGC_CHICK
AccessioniPrimary (citable) accession number: P05153
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 27, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families