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Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

PCK1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Enzyme regulationi

Activity is affected by a number of hormones regulating this metabolic process (such as glucagon, insulin, or glucocorticoids).

Pathway:igluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871SubstrateBy similarity
Metal bindingi244 – 2441ManganeseBy similarity
Metal bindingi264 – 2641Manganese; via tele nitrogenBy similarity
Binding sitei286 – 2861SubstrateBy similarity
Active sitei288 – 2881By similarity
Metal bindingi311 – 3111ManganeseBy similarity
Binding sitei405 – 4051GTPBy similarity
Binding sitei436 – 4361GTPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi287 – 2926GTPBy similarity
Nucleotide bindingi530 – 5334GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_115534. Gluconeogenesis.
SABIO-RKP05153.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32)
Short name:
PEPCK-C
Gene namesi
Name:PCK1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]PRO_0000103631Add
BLAST

Proteomic databases

PaxDbiP05153.
PRIDEiP05153.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000012342.

Structurei

3D structure databases

ProteinModelPortaliP05153.
SMRiP05153. Positions 13-622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni235 – 2373Substrate bindingBy similarity
Regioni403 – 4053Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiP05153.
KOiK01596.
PhylomeDBiP05153.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPELKTEVN IISKVIQGDL ESLPPQVREF IESNAKLCQP ESIHICDGSE
60 70 80 90 100
EENKKILDIM VEQGMIKKLS KYENCWLALT NPRDVARIES KTVIITQEQR
110 120 130 140 150
DTIPIPKTGS SQLGRWMSEE DFEKAFNTRF PGCMQGRTMY VIPFSMGPIG
160 170 180 190 200
SPLAKIGIEL TDSPYVVASM RMMTRMGTAA LKALGNGEFV KCLHSVGCPL
210 220 230 240 250
PLKEPLINNW PCNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRI
260 270 280 290 300
ASRIAKEEGW LAEHMLILGI TNPEGEKKYF AAAFPSACGK TNLAMMNPSR
310 320 330 340 350
PGWKIECVGD DIAWMKFDEL GNLRAINPEN GFFGVAPGTS IKTNPNAIKT
360 370 380 390 400
IFKNTIFTNV AETSDGGVYW EGIDEPLPPG VTLTSWKNKD WTPDNGEPCA
410 420 430 440 450
HPNSRFCTPA SQCPIMDPAW ESPEGVPIEG IIFGGRRPAG VPLVYEAFNW
460 470 480 490 500
QHGVFIGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM
510 520 530 540 550
AHRPAAKLPR IFHVNWFRKD SQGKFLWPGY GENSRVLEWM FNRIQGKASA
560 570 580 590 600
KSTAIGYIPA DTALNLKGLE DINLTELFNI SKEFWEKEVE EIKQYFEGQV
610 620
NADLPYEIER ELLALEMRIK QL
Length:622
Mass (Da):69,528
Last modified:August 13, 1987 - v1
Checksum:i1BB1E708A3D5E722
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14229 mRNA. Translation: AAA49005.1.
PIRiA26494. QYCHGC.
RefSeqiNP_990802.1. NM_205471.1.
UniGeneiGga.4447.

Genome annotation databases

GeneIDi396458.
KEGGigga:396458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14229 mRNA. Translation: AAA49005.1.
PIRiA26494. QYCHGC.
RefSeqiNP_990802.1. NM_205471.1.
UniGeneiGga.4447.

3D structure databases

ProteinModelPortaliP05153.
SMRiP05153. Positions 13-622.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000012342.

Proteomic databases

PaxDbiP05153.
PRIDEiP05153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396458.
KEGGigga:396458.

Organism-specific databases

CTDi5105.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiP05153.
KOiK01596.
PhylomeDBiP05153.

Enzyme and pathway databases

UniPathwayiUPA00138.
ReactomeiREACT_115534. Gluconeogenesis.
SABIO-RKP05153.

Miscellaneous databases

NextBioi20816499.
PROiP05153.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the mRNA encoding the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) from the chicken."
    Cook J.S., Weldon S.L., Garcia-Ruiz J.P., Hod Y., Hanson R.W.
    Proc. Natl. Acad. Sci. U.S.A. 83:7583-7587(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPCKGC_CHICK
AccessioniPrimary (citable) accession number: P05153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 22, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.