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P05153

- PCKGC_CHICK

UniProt

P05153 - PCKGC_CHICK

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Protein
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Gene
PCK1
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Enzyme regulationi

Activity is affected by a number of hormones regulating this metabolic process (such as glucagon, insulin, or glucocorticoids).UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871Substrate By similarity
Binding sitei237 – 2371Substrate; via amide nitrogen By similarity
Metal bindingi244 – 2441Manganese By similarity
Binding sitei244 – 2441Substrate By similarity
Metal bindingi264 – 2641Manganese; via tele nitrogen By similarity
Binding sitei286 – 2861Substrate By similarity
Active sitei288 – 2881 By similarity
Metal bindingi311 – 3111Manganese By similarity
Binding sitei405 – 4051GTP By similarity
Binding sitei436 – 4361GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi287 – 2926GTP By similarity
Nucleotide bindingi530 – 5334GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. gluconeogenesis Source: Reactome
  3. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_115534. Gluconeogenesis.
SABIO-RKP05153.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32)
Short name:
PEPCK-C
Gene namesi
Name:PCK1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytosol Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]UniRule annotation
PRO_0000103631Add
BLAST

Proteomic databases

PaxDbiP05153.
PRIDEiP05153.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000012342.

Structurei

3D structure databases

ProteinModelPortaliP05153.
SMRiP05153. Positions 13-622.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni403 – 4053Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
HOVERGENiHBG053651.
InParanoidiP05153.
KOiK01596.
PhylomeDBiP05153.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05153-1 [UniParc]FASTAAdd to Basket

« Hide

MAPELKTEVN IISKVIQGDL ESLPPQVREF IESNAKLCQP ESIHICDGSE    50
EENKKILDIM VEQGMIKKLS KYENCWLALT NPRDVARIES KTVIITQEQR 100
DTIPIPKTGS SQLGRWMSEE DFEKAFNTRF PGCMQGRTMY VIPFSMGPIG 150
SPLAKIGIEL TDSPYVVASM RMMTRMGTAA LKALGNGEFV KCLHSVGCPL 200
PLKEPLINNW PCNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRI 250
ASRIAKEEGW LAEHMLILGI TNPEGEKKYF AAAFPSACGK TNLAMMNPSR 300
PGWKIECVGD DIAWMKFDEL GNLRAINPEN GFFGVAPGTS IKTNPNAIKT 350
IFKNTIFTNV AETSDGGVYW EGIDEPLPPG VTLTSWKNKD WTPDNGEPCA 400
HPNSRFCTPA SQCPIMDPAW ESPEGVPIEG IIFGGRRPAG VPLVYEAFNW 450
QHGVFIGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM 500
AHRPAAKLPR IFHVNWFRKD SQGKFLWPGY GENSRVLEWM FNRIQGKASA 550
KSTAIGYIPA DTALNLKGLE DINLTELFNI SKEFWEKEVE EIKQYFEGQV 600
NADLPYEIER ELLALEMRIK QL 622
Length:622
Mass (Da):69,528
Last modified:August 13, 1987 - v1
Checksum:i1BB1E708A3D5E722
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14229 mRNA. Translation: AAA49005.1.
PIRiA26494. QYCHGC.
RefSeqiNP_990802.1. NM_205471.1.
UniGeneiGga.4447.

Genome annotation databases

GeneIDi396458.
KEGGigga:396458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M14229 mRNA. Translation: AAA49005.1 .
PIRi A26494. QYCHGC.
RefSeqi NP_990802.1. NM_205471.1.
UniGenei Gga.4447.

3D structure databases

ProteinModelPortali P05153.
SMRi P05153. Positions 13-622.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000012342.

Proteomic databases

PaxDbi P05153.
PRIDEi P05153.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 396458.
KEGGi gga:396458.

Organism-specific databases

CTDi 5105.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
HOVERGENi HBG053651.
InParanoidi P05153.
KOi K01596.
PhylomeDBi P05153.

Enzyme and pathway databases

UniPathwayi UPA00138 .
Reactomei REACT_115534. Gluconeogenesis.
SABIO-RK P05153.

Miscellaneous databases

NextBioi 20816499.
PROi P05153.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the mRNA encoding the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) from the chicken."
    Cook J.S., Weldon S.L., Garcia-Ruiz J.P., Hod Y., Hanson R.W.
    Proc. Natl. Acad. Sci. U.S.A. 83:7583-7587(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPCKGC_CHICK
AccessioniPrimary (citable) accession number: P05153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 3, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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