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P05153 (PCKGC_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Short name=PEPCK-C
EC=4.1.1.32
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:PCK1
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

Cofactor

Binds 1 manganese ion per subunit By similarity.

Enzyme regulation

Activity is affected by a number of hormones regulating this metabolic process (such as glucagon, insulin, or glucocorticoids).

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Subunit structure

Monomer.

Subcellular location

Cytoplasm.

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Traceable author statement. Source: Reactome

small molecule metabolic process

Traceable author statement. Source: Reactome

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
PRO_0000103631

Regions

Nucleotide binding287 – 2926GTP By similarity
Nucleotide binding530 – 5334GTP By similarity
Region403 – 4053Substrate binding By similarity

Sites

Active site2881 By similarity
Metal binding2441Manganese By similarity
Metal binding2641Manganese By similarity
Metal binding3111Manganese By similarity
Binding site871Substrate By similarity
Binding site2371Substrate; via amide nitrogen By similarity
Binding site2441Substrate By similarity
Binding site2861Substrate By similarity
Binding site4051GTP By similarity
Binding site4361GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
P05153 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 1BB1E708A3D5E722

FASTA62269,528
        10         20         30         40         50         60 
MAPELKTEVN IISKVIQGDL ESLPPQVREF IESNAKLCQP ESIHICDGSE EENKKILDIM 

        70         80         90        100        110        120 
VEQGMIKKLS KYENCWLALT NPRDVARIES KTVIITQEQR DTIPIPKTGS SQLGRWMSEE 

       130        140        150        160        170        180 
DFEKAFNTRF PGCMQGRTMY VIPFSMGPIG SPLAKIGIEL TDSPYVVASM RMMTRMGTAA 

       190        200        210        220        230        240 
LKALGNGEFV KCLHSVGCPL PLKEPLINNW PCNPELTLIA HLPDRREIIS FGSGYGGNSL 

       250        260        270        280        290        300 
LGKKCFALRI ASRIAKEEGW LAEHMLILGI TNPEGEKKYF AAAFPSACGK TNLAMMNPSR 

       310        320        330        340        350        360 
PGWKIECVGD DIAWMKFDEL GNLRAINPEN GFFGVAPGTS IKTNPNAIKT IFKNTIFTNV 

       370        380        390        400        410        420 
AETSDGGVYW EGIDEPLPPG VTLTSWKNKD WTPDNGEPCA HPNSRFCTPA SQCPIMDPAW 

       430        440        450        460        470        480 
ESPEGVPIEG IIFGGRRPAG VPLVYEAFNW QHGVFIGAAM RSEATAAAEH KGKIIMHDPF 

       490        500        510        520        530        540 
AMRPFFGYNF GKYLAHWLSM AHRPAAKLPR IFHVNWFRKD SQGKFLWPGY GENSRVLEWM 

       550        560        570        580        590        600 
FNRIQGKASA KSTAIGYIPA DTALNLKGLE DINLTELFNI SKEFWEKEVE EIKQYFEGQV 

       610        620 
NADLPYEIER ELLALEMRIK QL

« Hide

References

[1]"Nucleotide sequence of the mRNA encoding the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) from the chicken."
Cook J.S., Weldon S.L., Garcia-Ruiz J.P., Hod Y., Hanson R.W.
Proc. Natl. Acad. Sci. U.S.A. 83:7583-7587(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M14229 mRNA. Translation: AAA49005.1.
IPIIPI00585626.
PIRQYCHGC. A26494.
RefSeqNP_990802.1. NM_205471.1.
UniGeneGga.4447.

3D structure databases

ProteinModelPortalP05153.
SMRP05153. Positions 13-622.
ModBaseSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000012342.

Proteomic databases

PaxDbP05153.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396458.
KEGGgga:396458.

Organism-specific databases

CTD5105.

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
HOVERGENHBG053651.
InParanoidP05153.
KOK01596.
OrthoDBEOG47SSD8.

Enzyme and pathway databases

ReactomeREACT_115655. Metabolism.
SABIO-RKP05153.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20816499.

Entry information

Entry namePCKGC_CHICK
AccessionPrimary (citable) accession number: P05153
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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