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P05153

- PCKGC_CHICK

UniProt

P05153 - PCKGC_CHICK

Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

PCK1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

    Cofactori

    Binds 1 manganese ion per subunit.By similarity

    Enzyme regulationi

    Activity is affected by a number of hormones regulating this metabolic process (such as glucagon, insulin, or glucocorticoids).

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei237 – 2371Substrate; via amide nitrogenBy similarity
    Metal bindingi244 – 2441ManganeseBy similarity
    Binding sitei244 – 2441SubstrateBy similarity
    Metal bindingi264 – 2641Manganese; via tele nitrogenBy similarity
    Binding sitei286 – 2861SubstrateBy similarity
    Active sitei288 – 2881By similarity
    Metal bindingi311 – 3111ManganeseBy similarity
    Binding sitei405 – 4051GTPBy similarity
    Binding sitei436 – 4361GTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi287 – 2926GTPBy similarity
    Nucleotide bindingi530 – 5334GTPBy similarity

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. gluconeogenesis Source: Reactome
    3. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_115534. Gluconeogenesis.
    SABIO-RKP05153.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32)
    Short name:
    PEPCK-C
    Gene namesi
    Name:PCK1
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]PRO_0000103631Add
    BLAST

    Proteomic databases

    PaxDbiP05153.
    PRIDEiP05153.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000012342.

    Structurei

    3D structure databases

    ProteinModelPortaliP05153.
    SMRiP05153. Positions 13-622.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni403 – 4053Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    HOVERGENiHBG053651.
    InParanoidiP05153.
    KOiK01596.
    PhylomeDBiP05153.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05153-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPELKTEVN IISKVIQGDL ESLPPQVREF IESNAKLCQP ESIHICDGSE    50
    EENKKILDIM VEQGMIKKLS KYENCWLALT NPRDVARIES KTVIITQEQR 100
    DTIPIPKTGS SQLGRWMSEE DFEKAFNTRF PGCMQGRTMY VIPFSMGPIG 150
    SPLAKIGIEL TDSPYVVASM RMMTRMGTAA LKALGNGEFV KCLHSVGCPL 200
    PLKEPLINNW PCNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRI 250
    ASRIAKEEGW LAEHMLILGI TNPEGEKKYF AAAFPSACGK TNLAMMNPSR 300
    PGWKIECVGD DIAWMKFDEL GNLRAINPEN GFFGVAPGTS IKTNPNAIKT 350
    IFKNTIFTNV AETSDGGVYW EGIDEPLPPG VTLTSWKNKD WTPDNGEPCA 400
    HPNSRFCTPA SQCPIMDPAW ESPEGVPIEG IIFGGRRPAG VPLVYEAFNW 450
    QHGVFIGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM 500
    AHRPAAKLPR IFHVNWFRKD SQGKFLWPGY GENSRVLEWM FNRIQGKASA 550
    KSTAIGYIPA DTALNLKGLE DINLTELFNI SKEFWEKEVE EIKQYFEGQV 600
    NADLPYEIER ELLALEMRIK QL 622
    Length:622
    Mass (Da):69,528
    Last modified:August 13, 1987 - v1
    Checksum:i1BB1E708A3D5E722
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14229 mRNA. Translation: AAA49005.1.
    PIRiA26494. QYCHGC.
    RefSeqiNP_990802.1. NM_205471.1.
    UniGeneiGga.4447.

    Genome annotation databases

    GeneIDi396458.
    KEGGigga:396458.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14229 mRNA. Translation: AAA49005.1 .
    PIRi A26494. QYCHGC.
    RefSeqi NP_990802.1. NM_205471.1.
    UniGenei Gga.4447.

    3D structure databases

    ProteinModelPortali P05153.
    SMRi P05153. Positions 13-622.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000012342.

    Proteomic databases

    PaxDbi P05153.
    PRIDEi P05153.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396458.
    KEGGi gga:396458.

    Organism-specific databases

    CTDi 5105.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    HOVERGENi HBG053651.
    InParanoidi P05153.
    KOi K01596.
    PhylomeDBi P05153.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    Reactomei REACT_115534. Gluconeogenesis.
    SABIO-RK P05153.

    Miscellaneous databases

    NextBioi 20816499.
    PROi P05153.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the mRNA encoding the cytosolic form of phosphoenolpyruvate carboxykinase (GTP) from the chicken."
      Cook J.S., Weldon S.L., Garcia-Ruiz J.P., Hod Y., Hanson R.W.
      Proc. Natl. Acad. Sci. U.S.A. 83:7583-7587(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiPCKGC_CHICK
    AccessioniPrimary (citable) accession number: P05153
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3