Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ornithine carbamoyltransferase

Gene

ARG3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Enzyme regulationi

Forms a stable complex with CAR1 in the presence of ornithine and arginine. In this complex CAR1 retains activity, but ARG3 activity is inhibited.1 Publication

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (ARG3)
  2. Argininosuccinate synthase (ARG1)
  3. Argininosuccinate lyase (ARG4)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Carbamoyl phosphateBy similarity
Binding sitei118 – 1181OrnithineBy similarity
Binding sitei145 – 1451Carbamoyl phosphateBy similarity
Binding sitei185 – 1851OrnithineBy similarity
Active sitei289 – 2891
Binding sitei316 – 3161Carbamoyl phosphateBy similarity
Binding sitei316 – 3161OrnithineBy similarity

GO - Molecular functioni

  • amino acid binding Source: InterPro
  • ornithine carbamoyltransferase activity Source: SGD

GO - Biological processi

  • arginine biosynthetic process Source: SGD
  • arginine biosynthetic process via ornithine Source: GO_Central
  • urea cycle Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YJL088W-MONOMER.
YEAST:YJL088W-MONOMER.
ReactomeiR-SCE-70635. Urea cycle.
SABIO-RKP05150.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Alternative name(s):
Ornithine transcarbamylase
Short name:
OTCase
Gene namesi
Name:ARG3
Ordered Locus Names:YJL088W
ORF Names:J0924
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL088W.
SGDiS000003624. ARG3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • mitochondrion Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi68 – 681T → G: Reduces activity by 95%. Reduces affinity for ornithine 2-fold. 1 Publication
Mutagenesisi181 – 1811G → R: Loss of activity. 1 Publication
Mutagenesisi182 – 1821D → N: Reduces activity by 33%. Reduces affinity for ornithine 30-fold. 1 Publication
Mutagenesisi184 – 1841N → Q: Reduces activity by 50%. Reduces affinity for ornithine 20-fold. 1 Publication
Mutagenesisi185 – 1851N → Q: No effect on activity. Reduces affinity for ornithine 200-fold. 1 Publication
Mutagenesisi256 – 2561E → Q: Reduces activity by 50%. 1 Publication
Mutagenesisi263 – 2631K → A: Reduces activity by 70%. Reduces affinity for ornithine 18-fold. 1 Publication
Mutagenesisi289 – 2891C → S: Reduces activity by 90%. Reduces affinity for ornithine 6-fold. 1 Publication
Mutagenesisi290 – 2901L → S: Reduces activity by 86%. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 338337Ornithine carbamoyltransferasePRO_0000113082Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP05150.

Interactioni

Subunit structurei

Interacts with CAR1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CAR1P008124EBI-12712,EBI-2856

Protein-protein interaction databases

BioGridi33669. 23 interactions.
DIPiDIP-8093N.
IntActiP05150. 3 interactions.
MINTiMINT-396186.

Structurei

3D structure databases

ProteinModelPortaliP05150.
SMRiP05150. Positions 12-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 715Ornithine and carbamoyl phosphate bindingBy similarity
Regioni145 – 1484Ornithine and carbamoyl phosphate bindingBy similarity
Regioni249 – 2535Ornithine bindingBy similarity
Regioni288 – 2914Ornithine bindingBy similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

GeneTreeiENSGT00510000047417.
HOGENOMiHOG000022686.
InParanoidiP05150.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiEOG092C3SF9.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05150-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTASTPSS LRHLISIKDL SDEEFRILVQ RAQHFKNVFK ANKTNDFQSN
60 70 80 90 100
HLKLLGRTIA LIFTKRSTRT RISTEGAATF FGAQPMFLGK EDIQLGVNES
110 120 130 140 150
FYDTTKVVSS MVSCIFARVN KHEDILAFCK DSSVPIINSL CDKFHPLQAI
160 170 180 190 200
CDLLTIIENF NISLDEVNKG INSKLKMAWI GDANNVINDM CIACLKFGIS
210 220 230 240 250
VSISTPPGIE MDSDIVDEAK KVAERNGATF ELTHDSLKAS TNANILVTDT
260 270 280 290 300
FVSMGEEFAK QAKLKQFKGF QINQELVSVA DPNYKFMHCL PRHQEEVSDD
310 320 330
VFYGEHSIVF EEAENRLYAA MSAIDIFVNN KGNFKDLK
Length:338
Mass (Da):37,845
Last modified:August 13, 1987 - v1
Checksum:i6EBB6598FA93C59E
GO

Sequence cautioni

The sequence CAA58480 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA89381 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11946 Genomic DNA. Translation: AAA34432.1.
X83502 Genomic DNA. Translation: CAA58480.1. Different initiation.
Z49363 Genomic DNA. Translation: CAA89381.1. Different initiation.
M28301 Genomic DNA. Translation: AAA34433.1.
BK006943 Genomic DNA. Translation: DAA08712.1.
PIRiS56020. OWBY.
RefSeqiNP_012447.1. NM_001181521.1.

Genome annotation databases

EnsemblFungiiYJL088W; YJL088W; YJL088W.
GeneIDi853357.
KEGGisce:YJL088W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11946 Genomic DNA. Translation: AAA34432.1.
X83502 Genomic DNA. Translation: CAA58480.1. Different initiation.
Z49363 Genomic DNA. Translation: CAA89381.1. Different initiation.
M28301 Genomic DNA. Translation: AAA34433.1.
BK006943 Genomic DNA. Translation: DAA08712.1.
PIRiS56020. OWBY.
RefSeqiNP_012447.1. NM_001181521.1.

3D structure databases

ProteinModelPortaliP05150.
SMRiP05150. Positions 12-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33669. 23 interactions.
DIPiDIP-8093N.
IntActiP05150. 3 interactions.
MINTiMINT-396186.

Proteomic databases

MaxQBiP05150.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL088W; YJL088W; YJL088W.
GeneIDi853357.
KEGGisce:YJL088W.

Organism-specific databases

EuPathDBiFungiDB:YJL088W.
SGDiS000003624. ARG3.

Phylogenomic databases

GeneTreeiENSGT00510000047417.
HOGENOMiHOG000022686.
InParanoidiP05150.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiEOG092C3SF9.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.
BioCyciMetaCyc:YJL088W-MONOMER.
YEAST:YJL088W-MONOMER.
ReactomeiR-SCE-70635. Urea cycle.
SABIO-RKP05150.

Miscellaneous databases

PROiP05150.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_YEAST
AccessioniPrimary (citable) accession number: P05150
Secondary accession number(s): D6VW96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 7, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.