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Protein

cAMP-dependent protein kinase catalytic subunit beta

Gene

PRKACB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, and differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by cAMP.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73ATPPROSITE-ProRule annotation1
Active sitei167Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi50 – 58ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, cAMP, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.11 908
ReactomeiR-BTA-163615 PKA activation
R-BTA-164378 PKA activation in glucagon signalling
R-BTA-180024 DARPP-32 events
R-BTA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-BTA-392517 Rap1 signalling
R-BTA-422356 Regulation of insulin secretion
R-BTA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-BTA-4420097 VEGFA-VEGFR2 Pathway
R-BTA-442720 CREB phosphorylation through the activation of Adenylate Cyclase
R-BTA-5610785 GLI3 is processed to GLI3R by the proteasome
R-BTA-5610787 Hedgehog 'off' state
R-BTA-5621575 CD209 (DC-SIGN) signaling
R-BTA-5687128 MAPK6/MAPK4 signaling
R-BTA-8853659 RET signaling
R-BTA-8963896 HDL assembly
R-BTA-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase catalytic subunit beta (EC:2.7.11.11)
Short name:
PKA C-beta
Gene namesi
Name:PRKACB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Organism-specific databases

VGNCiVGNC:33321 PRKACB

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2111446

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000860572 – 351cAMP-dependent protein kinase catalytic subunit betaAdd BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei3Deamidated asparagine; partial3 Publications1
Modified residuei11PhosphoserineBy similarity1
Modified residuei69PhosphotyrosineBy similarity1
Modified residuei140PhosphoserineBy similarity1
Modified residuei198PhosphothreonineBy similarity1
Modified residuei322PhosphoserineBy similarity1
Modified residuei331PhosphotyrosineBy similarity1
Modified residuei339PhosphoserineBy similarity1

Post-translational modificationi

Asn-3 is deaminated to Asp in more than 25% of the proteins, giving rise to 2 major isoelectric variants, called CB and CA respectively (0.4 pH unit change). Deamidation proceeds via the so-called beta-aspartyl shift mechanism and yields either 'D-Asp-2' (major) or 'D-isoAsp-2' (minor), in addition to L-isomers. Deamidation occurs after the addition of myristate. The Asn-3 form reaches a significantly larger nuclear/cytoplasmic ratio than the 'Asp-2' form.3 Publications

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiP05131
PRIDEiP05131

PTM databases

iPTMnetiP05131

Expressioni

Tissue specificityi

Isoform 2 is mainly expressed in heart and brain.1 Publication

Gene expression databases

BgeeiENSBTAG00000011953
ExpressionAtlasiP05131 baseline and differential

Interactioni

Subunit structurei

A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The cAMP-dependent protein kinase catalytic subunit binds PJA2. Interacts with GPKOW.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi159585, 26 interactors
IntActiP05131, 27 interactors
STRINGi9913.ENSBTAP00000046803

Structurei

3D structure databases

ProteinModelPortaliP05131
SMRiP05131
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 298Protein kinasePROSITE-ProRule annotationAdd BLAST255
Domaini299 – 351AGC-kinase C-terminalAdd BLAST53

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0616 Eukaryota
ENOG410XPQQ LUCA
GeneTreeiENSGT00810000125385
HOVERGENiHBG108317
InParanoidiP05131
KOiK04345
OMAiGKEFCEF
OrthoDBiEOG091G10O8
TreeFamiTF313399

Family and domain databases

InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: N-terminal differences in alternatively spliced products could be involved in regulation such as alternative targeting.
Isoform 1 (identifier: P05131-1) [UniParc]FASTAAdd to basket
Also known as: Beta-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNAATAKKG SEVESVKEFL AKAKEDFLKK WENPAPNNAG LEDFERKKTL
60 70 80 90 100
GTGSFGRVML VKHKATEQYY AMKILDKQKV VKLKQIEHTL NEKRILQAVN
110 120 130 140 150
FPFLVRLEYA FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ
160 170 180 190 200
IVLTFEYLHS LDLIYRDLKP ENLLIDHQGY IQVTDFGFAK RVKGRTWTLC
210 220 230 240 250
GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK
260 270 280 290 300
IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT
310 320 330 340 350
TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEDIRVSI TEKCGKEFCE

F
Length:351
Mass (Da):40,594
Last modified:January 23, 2007 - v2
Checksum:iC84D84C2722C9F0C
GO
Isoform 2 (identifier: P05131-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: Beta-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MGNAATAKKGSEVESV → MAAYREVPCN...EHTALWDRSM

Show »
Length:397
Mass (Da):46,108
Checksum:i7F87EF506C32FE29
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0312461 – 16MGNAA…EVESV → MAAYREVPCNQYTGTTALQK LEGFASRLFHRHSKGTAHDQ KTALENDSLHFSEHTALWDR SM in isoform 2. 1 PublicationAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02647 mRNA Translation: AAA30707.1
M60482 mRNA Translation: AAA30424.1
PIRiA23716 OKBOB2
A25334 OKBOB1
RefSeqiNP_777010.1, NM_174585.3 [P05131-1]
XP_005204430.1, XM_005204373.3 [P05131-2]
UniGeneiBt.391

Genome annotation databases

EnsembliENSBTAT00000044032; ENSBTAP00000041551; ENSBTAG00000011953 [P05131-1]
ENSBTAT00000050033; ENSBTAP00000046803; ENSBTAG00000011953 [P05131-2]
GeneIDi282323
KEGGibta:282323

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKAPCB_BOVIN
AccessioniPrimary (citable) accession number: P05131
Secondary accession number(s): P24256
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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