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Protein

Protein kinase C, brain isozyme

Gene

Pkc53E

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei379 – 3791ATPPROSITE-ProRule annotation
Active sitei474 – 4741Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri45 – 10460Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri119 – 16951Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi356 – 3649ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-dependent protein kinase C activity Source: FlyBase
  • diacylglycerol binding Source: FlyBase
  • protein kinase C activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase
  • zinc ion binding Source: InterPro

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • positive regulation of clathrin-mediated endocytosis Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of hemocyte proliferation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.13. 1994.
ReactomeiR-DME-111933. Calmodulin induced events.
R-DME-114516. Disinhibition of SNARE formation.
R-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1433559. Regulation of KIT signaling.
R-DME-2179392. EGFR Transactivation by Gastrin.
R-DME-3000170. Syndecan interactions.
R-DME-399997. Acetylcholine regulates insulin secretion.
R-DME-416993. Trafficking of GluR2-containing AMPA receptors.
R-DME-4419969. Depolymerisation of the Nuclear Lamina.
R-DME-450520. HuR (ELAVL1) binds and stabilizes mRNA.
R-DME-5218921. VEGFR2 mediated cell proliferation.
R-DME-76005. Response to elevated platelet cytosolic Ca2+.
SignaLinkiP05130.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C, brain isozyme (EC:2.7.11.13)
Short name:
PKC
Alternative name(s):
dPKC53E(BR)
Gene namesi
Name:Pkc53E
Synonyms:PKC1
ORF Names:CG6622
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003091. Pkc53E.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 679679Protein kinase C, brain isozymePRO_0000055730Add
BLAST

Proteomic databases

PaxDbiP05130.

Expressioni

Tissue specificityi

Head neural tissue.

Gene expression databases

BgeeiP05130.
ExpressionAtlasiP05130. differential.
GenevisibleiP05130. DM.

Interactioni

Protein-protein interaction databases

BioGridi71309. 1 interaction.
IntActiP05130. 5 interactions.
STRINGi7227.FBpp0086197.

Structurei

3D structure databases

ProteinModelPortaliP05130.
SMRiP05130. Positions 46-676.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini191 – 27888C2PROSITE-ProRule annotationAdd
BLAST
Domaini350 – 608259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini609 – 67971AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri45 – 10460Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri119 – 16951Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
InParanoidiP05130.
KOiK02677.
OMAiQSKKDMI.
OrthoDBiEOG77M8QM.
PhylomeDBiP05130.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000550. PKC_alpha. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P05130-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEGSDNNGD PQQQGAEGEA VGENKMKSRL RKGALKKKNV FNVKDHCFIA
60 70 80 90 100
RFFKQPTFCS HCKDFICGYQ SGYAWMGFGK QGFQCQVCSY VVHKRCHEYV
110 120 130 140 150
TFICPGKDKG IDSDSPKTQH NFEPFTYAGP TFCDHCGSLL YGIYHQGLKC
160 170 180 190 200
SACDMNVHAR CKENVPSLCG CDHTERRGRI YLEINVKENL LTVQIKEGRN
210 220 230 240 250
LIPMDPNGLS DPYVKVKLIP DDKDQSKKKT RTIKACLNPV WNETLTYDLK
260 270 280 290 300
PEDKDRRILI EVWDWDRTSR NDFMGALSFG ISEIIKNPTN GWFKLLTQDE
310 320 330 340 350
GEYYNVPCAD DEQDLLKLKQ KPSQKKPMVM RSDTNTHTSS KKDMIRATDF
360 370 380 390 400
NFIKVLGKGS FGKVLLAERK GSEELYAIKI LKKDVIIQDD DVECTMIEKR
410 420 430 440 450
VLALGEKPPF LVQLHSCFQT MDRLFFVMEY VNGGDLMFQI QQFGKFKEPV
460 470 480 490 500
AVFYAAEIAA GLFFLHTKGI LYRDLKLDNV LLDADGHVKI ADFGMCKENI
510 520 530 540 550
VGDKTTKTFC GTPDYIAPEI ILYQPYGKSV DWWAYGVLLY EMLVGQPPFD
560 570 580 590 600
GEDEEELFAA ITDHNVSYPK SLSKEAKEAC KGFLTKQPNK RLGCGSSGEE
610 620 630 640 650
DVRLHPFFRR IDWEKIENRE VQPPFKPKIK HRKDVSNFDK QFTSEKTDLT
660 670
PTDKVFMMNL DQSEFVGFSY MNPEYVFSP
Length:679
Mass (Da):77,695
Last modified:November 28, 2002 - v2
Checksum:i3C69AD351E36B7DC
GO
Isoform Short (identifier: P05130-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     67-77: CGYQSGYAWMG → WG

Note: No experimental confirmation available.
Show »
Length:670
Mass (Da):76,734
Checksum:i588F5194A212BEAF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti608 – 6081F → S in CAA28736 (PubMed:3107983).Curated
Sequence conflicti608 – 6081F → S in CAA28890 (PubMed:3107983).Curated
Sequence conflicti634 – 64815DVSNF…SEKTD → MCPTLTSSSHQRKQT (PubMed:3107983).CuratedAdd
BLAST
Sequence conflicti649 – 67931Missing (PubMed:3107983).CuratedAdd
BLAST

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti437 – 4371M → I.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei67 – 7711CGYQSGYAWMG → WG in isoform Short. 1 PublicationVSP_004743Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05076 mRNA. Translation: CAA28736.1.
X05279
, X05280, X05281, X05282, X05283 Genomic DNA. Translation: CAA28890.2.
AE013599 Genomic DNA. Translation: AAF57932.1.
AE013599 Genomic DNA. Translation: AAF57933.1.
AY095003 mRNA. Translation: AAM11331.1.
PIRiA32545.
RefSeqiNP_001261035.1. NM_001274106.1. [P05130-2]
NP_476682.1. NM_057334.3. [P05130-2]
NP_725626.1. NM_166201.3. [P05130-1]
UniGeneiDm.3632.

Genome annotation databases

EnsemblMetazoaiFBtr0087048; FBpp0086197; FBgn0003091. [P05130-1]
GeneIDi48311.
KEGGidme:Dmel_CG6622.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05076 mRNA. Translation: CAA28736.1.
X05279
, X05280, X05281, X05282, X05283 Genomic DNA. Translation: CAA28890.2.
AE013599 Genomic DNA. Translation: AAF57932.1.
AE013599 Genomic DNA. Translation: AAF57933.1.
AY095003 mRNA. Translation: AAM11331.1.
PIRiA32545.
RefSeqiNP_001261035.1. NM_001274106.1. [P05130-2]
NP_476682.1. NM_057334.3. [P05130-2]
NP_725626.1. NM_166201.3. [P05130-1]
UniGeneiDm.3632.

3D structure databases

ProteinModelPortaliP05130.
SMRiP05130. Positions 46-676.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi71309. 1 interaction.
IntActiP05130. 5 interactions.
STRINGi7227.FBpp0086197.

Proteomic databases

PaxDbiP05130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087048; FBpp0086197; FBgn0003091. [P05130-1]
GeneIDi48311.
KEGGidme:Dmel_CG6622.

Organism-specific databases

CTDi48311.
FlyBaseiFBgn0003091. Pkc53E.

Phylogenomic databases

eggNOGiKOG0694. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126964.
InParanoidiP05130.
KOiK02677.
OMAiQSKKDMI.
OrthoDBiEOG77M8QM.
PhylomeDBiP05130.

Enzyme and pathway databases

BRENDAi2.7.11.13. 1994.
ReactomeiR-DME-111933. Calmodulin induced events.
R-DME-114516. Disinhibition of SNARE formation.
R-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1433559. Regulation of KIT signaling.
R-DME-2179392. EGFR Transactivation by Gastrin.
R-DME-3000170. Syndecan interactions.
R-DME-399997. Acetylcholine regulates insulin secretion.
R-DME-416993. Trafficking of GluR2-containing AMPA receptors.
R-DME-4419969. Depolymerisation of the Nuclear Lamina.
R-DME-450520. HuR (ELAVL1) binds and stabilizes mRNA.
R-DME-5218921. VEGFR2 mediated cell proliferation.
R-DME-76005. Response to elevated platelet cytosolic Ca2+.
SignaLinkiP05130.

Miscellaneous databases

GenomeRNAii48311.
PROiP05130.

Gene expression databases

BgeeiP05130.
ExpressionAtlasiP05130. differential.
GenevisibleiP05130. DM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000550. PKC_alpha. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and nucleotide sequence of a Drosophila melanogaster protein kinase C gene."
    Rosenthal A., Rhee L., Yadegari R., Paro R., Ullrich A., Goeddel D.V.
    EMBO J. 6:433-441(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT).
    Strain: Canton-S and Oregon-R.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
    Strain: Berkeley.
    Tissue: Ovary.

Entry informationi

Entry nameiKPC1_DROME
AccessioniPrimary (citable) accession number: P05130
Secondary accession number(s): Q9V7V6, Q9V7V7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 28, 2002
Last modified: July 6, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
The sequence shown is that of Oregon-R.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.