Reviewed, UniProtKB/Swiss-Prot P05130 (KPC1_DROME)

Names and origin

Protein names

Recommended name:
    Protein kinase C, brain isozyme
      Short name=PKC
    EC=2.7.11.13
Alternative name(s):
    dPKC53E(BR)

Gene names

Name:	Pkc53E
Synonyms:	PKC1
ORF Names:	CG6622

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	679 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Head neural tissue.
Miscellaneous	This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. The sequence shown is that of Oregon-R.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Calcium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from direct assay. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P05130-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P05130-2)

The sequence of this isoform differs from the canonical sequence as follows:
     67-77: CGYQSGYAWMG → WG

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 679	679	Protein kinase C, brain isozyme		PRO_0000055730
Regions
	Domain	191 – 278	88	C2
	Domain	350 – 608	259	Protein kinase
	Domain	609 – 679	71	AGC-kinase C-terminal
	Zinc finger	45 – 104	60	Phorbol-ester/DAG-type 1
	Zinc finger	119 – 169	51	Phorbol-ester/DAG-type 2
	Nucleotide binding	356 – 364	9	ATP By similarity
Sites
	Active site	474	1	Proton acceptor By similarity
	Binding site	379	1	ATP By similarity
Natural variations
	Alternative sequence	67 – 77	11	CGYQSGYAWMG → WG in isoform Short.		VSP_004743
	Natural variant	437	1	M → I
Experimental info
	Sequence conflict	608	1	F → S in CAA28736. Ref.1
	Sequence conflict	608	1	F → S in CAA28890. Ref.1
	Sequence conflict	634 – 648	15	DVSNF…SEKTD → MCPTLTSSSHQRKQT Ref.1
	Sequence conflict	649 – 679	31	Missing Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 28, 2002. Version 2. Checksum: 3C69AD351E36B7DC	FASTA	679	77,695
	10 20 30 40 50 60 MSEGSDNNGD PQQQGAEGEA VGENKMKSRL RKGALKKKNV FNVKDHCFIA RFFKQPTFCS 70 80 90 100 110 120 HCKDFICGYQ SGYAWMGFGK QGFQCQVCSY VVHKRCHEYV TFICPGKDKG IDSDSPKTQH 130 140 150 160 170 180 NFEPFTYAGP TFCDHCGSLL YGIYHQGLKC SACDMNVHAR CKENVPSLCG CDHTERRGRI 190 200 210 220 230 240 YLEINVKENL LTVQIKEGRN LIPMDPNGLS DPYVKVKLIP DDKDQSKKKT RTIKACLNPV 250 260 270 280 290 300 WNETLTYDLK PEDKDRRILI EVWDWDRTSR NDFMGALSFG ISEIIKNPTN GWFKLLTQDE 310 320 330 340 350 360 GEYYNVPCAD DEQDLLKLKQ KPSQKKPMVM RSDTNTHTSS KKDMIRATDF NFIKVLGKGS 370 380 390 400 410 420 FGKVLLAERK GSEELYAIKI LKKDVIIQDD DVECTMIEKR VLALGEKPPF LVQLHSCFQT 430 440 450 460 470 480 MDRLFFVMEY VNGGDLMFQI QQFGKFKEPV AVFYAAEIAA GLFFLHTKGI LYRDLKLDNV 490 500 510 520 530 540 LLDADGHVKI ADFGMCKENI VGDKTTKTFC GTPDYIAPEI ILYQPYGKSV DWWAYGVLLY 550 560 570 580 590 600 EMLVGQPPFD GEDEEELFAA ITDHNVSYPK SLSKEAKEAC KGFLTKQPNK RLGCGSSGEE 610 620 630 640 650 660 DVRLHPFFRR IDWEKIENRE VQPPFKPKIK HRKDVSNFDK QFTSEKTDLT PTDKVFMMNL 670 DQSEFVGFSY MNPEYVFSP
	Isoform Short. Checksum: 588F5194A212BEAF	FASTA	670	76,734
	10 20 30 40 50 60 MSEGSDNNGD PQQQGAEGEA VGENKMKSRL RKGALKKKNV FNVKDHCFIA RFFKQPTFCS 70 80 90 100 110 120 HCKDFIWGFG KQGFQCQVCS YVVHKRCHEY VTFICPGKDK GIDSDSPKTQ HNFEPFTYAG 130 140 150 160 170 180 PTFCDHCGSL LYGIYHQGLK CSACDMNVHA RCKENVPSLC GCDHTERRGR IYLEINVKEN 190 200 210 220 230 240 LLTVQIKEGR NLIPMDPNGL SDPYVKVKLI PDDKDQSKKK TRTIKACLNP VWNETLTYDL 250 260 270 280 290 300 KPEDKDRRIL IEVWDWDRTS RNDFMGALSF GISEIIKNPT NGWFKLLTQD EGEYYNVPCA 310 320 330 340 350 360 DDEQDLLKLK QKPSQKKPMV MRSDTNTHTS SKKDMIRATD FNFIKVLGKG SFGKVLLAER 370 380 390 400 410 420 KGSEELYAIK ILKKDVIIQD DDVECTMIEK RVLALGEKPP FLVQLHSCFQ TMDRLFFVME 430 440 450 460 470 480 YVNGGDLMFQ IQQFGKFKEP VAVFYAAEIA AGLFFLHTKG ILYRDLKLDN VLLDADGHVK 490 500 510 520 530 540 IADFGMCKEN IVGDKTTKTF CGTPDYIAPE IILYQPYGKS VDWWAYGVLL YEMLVGQPPF 550 560 570 580 590 600 DGEDEEELFA AITDHNVSYP KSLSKEAKEA CKGFLTKQPN KRLGCGSSGE EDVRLHPFFR 610 620 630 640 650 660 RIDWEKIENR EVQPPFKPKI KHRKDVSNFD KQFTSEKTDL TPTDKVFMMN LDQSEFVGFS 670 YMNPEYVFSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and nucleotide sequence of a Drosophila melanogaster protein kinase C gene." Rosenthal A., Rhee L., Yadegari R., Paro R., Ullrich A., Goeddel D.V. EMBO J. 6:433-441(1987) [PubMed: 3107983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM SHORT). Strain: Canton-S and Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Strain: Berkeley. Tissue: Ovary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X05076 mRNA. Translation: CAA28736.1. X05279 , X05280, X05281, X05282, X05283 Genomic DNA. Translation: CAA28890.2. AE013599 Genomic DNA. Translation: AAF57932.1. AE013599 Genomic DNA. Translation: AAF57933.1. AY095003 mRNA. Translation: AAM11331.1.
PIR	A32545.
RefSeq	NP_476682.1. NP_725626.1.
UniGene	Dm.3632
3D structure databases
HSSP	HSSP built from PDB template 1DSY based on UniProtKB P05696.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05130. 2 interactions.
STRING	P05130.
Genome annotation databases
Ensembl	FBtr0087048; FBpp0086197; FBgn0003091; Drosophila melanogaster. [Genome view]
GeneID	48311.
Organism-specific databases
CTD	48311.
FlyBase	FBgn0003091. Pkc53E.
Phylogenomic databases
HOGENOM	P05130.
OMA	EEGEFYN.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-005054-MON.
BRENDA	2.7.11.13. 48.
Gene expression databases
GermOnline	CG6622. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000008. C2_Ca-dep. IPR018029. C2_membr_targeting. IPR020477. C2_region. IPR020454. DAG/PE_bd. IPR015745. PKC. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR014375. Protein_kinase_C_a/b/g. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00168. C2. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000550. PKC_alpha. 1 hit.
PRINTS	PR00360. C2DOMAIN. PR00008. DAGPEDOMAIN.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 2 hits. SM00239. C2. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50004. C2. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	839293.

Entry information

Entry name

KPC1_DROME

Accession

Primary (citable) accession number: P05130
Secondary accession number(s): Q9V7V6, Q9V7V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	November 28, 2002
Last modified:	November 3, 2009
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project