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P05126

- KPCB_BOVIN

UniProt

P05126 - KPCB_BOVIN

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Protein

Protein kinase C beta type

Gene
PRKCB, PRKCB1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Binds 3 calcium ions per subunit. The ions are bound to the C2 domain By similarity.

Enzyme regulationi

Classical (or conventional) PKCs (PRKCA, PRKCB and PRKCG) are activated by calcium and diacylglycerol (DAG) in the presence of phosphatidylserine. Three specific sites; Thr-500 (activation loop of the kinase domain), Thr-642 (turn motif) and Ser-661 (hydrophobic region), need to be phosphorylated for its full activation. Specifically inhibited by enzastaurin (LY317615) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi186 – 1861Calcium 1; via carbonyl oxygen By similarity
Metal bindingi187 – 1871Calcium 1 By similarity
Metal bindingi187 – 1871Calcium 2 By similarity
Metal bindingi193 – 1931Calcium 2 By similarity
Metal bindingi246 – 2461Calcium 1 By similarity
Metal bindingi246 – 2461Calcium 2 By similarity
Metal bindingi247 – 2471Calcium 2; via carbonyl oxygen By similarity
Metal bindingi248 – 2481Calcium 1 By similarity
Metal bindingi248 – 2481Calcium 2 By similarity
Metal bindingi248 – 2481Calcium 3 By similarity
Metal bindingi251 – 2511Calcium 3 By similarity
Metal bindingi252 – 2521Calcium 3; via carbonyl oxygen By similarity
Metal bindingi254 – 2541Calcium 1 By similarity
Metal bindingi254 – 2541Calcium 3 By similarity
Binding sitei371 – 3711ATP By similarity
Active sitei466 – 4661Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri36 – 8651Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri101 – 15151Phorbol-ester/DAG-type 2Add
BLAST
Nucleotide bindingi348 – 3569ATP By similarity

GO - Molecular functioni

  1. androgen receptor binding Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. calcium channel regulator activity Source: Ensembl
  4. chromatin binding Source: UniProtKB
  5. histone binding Source: UniProtKB
  6. histone kinase activity (H3-T6 specific) Source: UniProtKB
  7. ligand-dependent nuclear receptor transcription coactivator activity Source: UniProtKB
  8. protein kinase C activity Source: UniProtKB-EC
  9. zinc ion binding Source: InterPro

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. B cell activation Source: UniProtKB
  3. B cell receptor signaling pathway Source: UniProtKB
  4. calcium ion transport Source: Ensembl
  5. cellular calcium ion homeostasis Source: Ensembl
  6. cellular response to carbohydrate stimulus Source: Ensembl
  7. histone H3-T6 phosphorylation Source: UniProtKB
  8. intracellular signal transduction Source: InterPro
  9. negative regulation of glucose transport Source: UniProtKB
  10. negative regulation of insulin receptor signaling pathway Source: UniProtKB
  11. positive regulation of angiogenesis Source: UniProtKB
  12. positive regulation of B cell receptor signaling pathway Source: UniProtKB
  13. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  14. positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  15. positive regulation of vascular endothelial growth factor receptor signaling pathway Source: UniProtKB
  16. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  17. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Adaptive immunity, Apoptosis, Immunity, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.11.13. 908.
ReactomeiREACT_205897. Activation of NF-kappaB in B cells.
REACT_209033. G alpha (z) signalling events.
REACT_212308. Response to elevated platelet cytosolic Ca2+.
REACT_217496. Trafficking of GluR2-containing AMPA receptors.
REACT_217562. WNT5A-dependent internalization of FZD4.
REACT_220570. Disinhibition of SNARE formation.
REACT_221009. Depolymerisation of the Nuclear Lamina.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C beta type (EC:2.7.11.13)
Short name:
PKC-B
Short name:
PKC-beta
Gene namesi
Name:PRKCB
Synonyms:PRKCB1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 25

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Membrane; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB
  3. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 671670Protein kinase C beta typePRO_0000055683Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei16 – 161Phosphoserine; by autocatalysis Reviewed prediction
Modified residuei17 – 171Phosphothreonine; by autocatalysis Reviewed prediction
Modified residuei250 – 2501Phosphothreonine; by autocatalysis By similarity
Modified residuei324 – 3241Phosphothreonine; by autocatalysis Reviewed prediction
Modified residuei500 – 5001Phosphothreonine; by PDPK1 By similarity
Modified residuei504 – 5041Phosphothreonine By similarity
Modified residuei635 – 6351Phosphothreonine; by autocatalysis By similarity
Modified residuei642 – 6421Phosphothreonine; by autocatalysis By similarity
Modified residuei661 – 6611Phosphoserine; by autocatalysis By similarity
Modified residuei662 – 6621Phosphotyrosine; by SYK By similarity

Post-translational modificationi

Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP05126.

Interactioni

Subunit structurei

Interacts with PDK1. Interacts in vitro with PRKCBP1. Interacts with PHLPP1 and PHLPP2; both proteins mediate its dephosphorylation. Interacts with KDM1A/LSD1, PKN1 and ANDR By similarity.

Protein-protein interaction databases

BioGridi159587. 1 interaction.
STRINGi9913.ENSBTAP00000027870.

Structurei

3D structure databases

ProteinModelPortaliP05126.
SMRiP05126. Positions 37-288, 339-668.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini173 – 26088C2Add
BLAST
Domaini342 – 600259Protein kinaseAdd
BLAST
Domaini601 – 66969AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri36 – 8651Phorbol-ester/DAG-type 1Add
BLAST
Zinc fingeri101 – 15151Phorbol-ester/DAG-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117339.
HOGENOMiHOG000233022.
HOVERGENiHBG108317.
InParanoidiP05126.
KOiK02677.
OMAiQAHIDRE.
OrthoDBiEOG77M8QM.
TreeFamiTF351133.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000550. PKC_alpha. 1 hit.
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Beta-I (identifier: P05126-1) [UniParc]FASTAAdd to Basket

Also known as: PRKCB1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MADPAAGPPP SEGEESTVRF ARKGALRQKN VHEVKNHKFT ARFFKQPTFC    50
SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPASDDPRS 100
KHKFKIHTYS SPTFCDHCGS LLYGLIHQGM KCDTCMMNVH KRCVMNVPSL 150
CGTDHTERRG RIYIQAHIER EVLIVVVRDA KNLVPMDPNG LSDPYVKLKL 200
IPDPKSESKQ KTKTIKCSLN PEWNETFRFQ LKESDKDRRL SVEIWDWDLT 250
SRNDFMGSLS FGISELQKAG VDGWFKLLSQ EEGEYFNVPV PPEGSEGNEE 300
LRQKFERAKI GPGPKTPEEK TTNTISKFDN NGNRDRMKLT DFNFLMVLGK 350
GSFGKVMLSE RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP 400
PFLTQLHSCF QTMDRLYFVM EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI 450
AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI KIADFGMCKE NIWDGVTTKT 500
FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP FEGEDEDELF 550
QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKEHAFF 600
RYIDWEKLER KEIQPPYKPK ARDKRDTSNF DKEFTRQPVE LTPTDKLFIM 650
NLDQNEFAGF SYTNPEFVIN V 671
Length:671
Mass (Da):76,790
Last modified:May 18, 2010 - v4
Checksum:i1F31D6A9D3C4C61F
GO
Isoform Beta-II (identifier: P05126-2) [UniParc]FASTAAdd to Basket

Also known as: PRKCB2

The sequence of this isoform differs from the canonical sequence as follows:
     622-671: RDKRDTSNFD...YTNPEFVINV → CGRNAENFDR...SEFLKPEVKS

Show »
Length:673
Mass (Da):76,933
Checksum:i61711D08707B005B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei622 – 67150RDKRD…FVINV → CGRNAENFDRFFTRHPPVLT PPDQEVIRNIDQSEFEGFSF VNSEFLKPEVKS in isoform Beta-II. VSP_039222Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti576 – 5761M → I in AAA30703. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13974 mRNA. Translation: AAA30703.1.
BC140620 mRNA. Translation: AAI40621.1.
PIRiA24664. KIBOC2.
RefSeqiNP_777012.1. NM_174587.1.
XP_005224747.1. XM_005224690.1. [P05126-1]
UniGeneiBt.5435.

Genome annotation databases

EnsembliENSBTAT00000027870; ENSBTAP00000027870; ENSBTAG00000020921. [P05126-2]
ENSBTAT00000042942; ENSBTAP00000040548; ENSBTAG00000020921. [P05126-1]
GeneIDi282325.
KEGGibta:282325.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13974 mRNA. Translation: AAA30703.1 .
BC140620 mRNA. Translation: AAI40621.1 .
PIRi A24664. KIBOC2.
RefSeqi NP_777012.1. NM_174587.1.
XP_005224747.1. XM_005224690.1. [P05126-1 ]
UniGenei Bt.5435.

3D structure databases

ProteinModelPortali P05126.
SMRi P05126. Positions 37-288, 339-668.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 159587. 1 interaction.
STRINGi 9913.ENSBTAP00000027870.

Proteomic databases

PRIDEi P05126.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000027870 ; ENSBTAP00000027870 ; ENSBTAG00000020921 . [P05126-2 ]
ENSBTAT00000042942 ; ENSBTAP00000040548 ; ENSBTAG00000020921 . [P05126-1 ]
GeneIDi 282325.
KEGGi bta:282325.

Organism-specific databases

CTDi 5579.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117339.
HOGENOMi HOG000233022.
HOVERGENi HBG108317.
InParanoidi P05126.
KOi K02677.
OMAi QAHIDRE.
OrthoDBi EOG77M8QM.
TreeFami TF351133.

Enzyme and pathway databases

BRENDAi 2.7.11.13. 908.
Reactomei REACT_205897. Activation of NF-kappaB in B cells.
REACT_209033. G alpha (z) signalling events.
REACT_212308. Response to elevated platelet cytosolic Ca2+.
REACT_217496. Trafficking of GluR2-containing AMPA receptors.
REACT_217562. WNT5A-dependent internalization of FZD4.
REACT_220570. Disinhibition of SNARE formation.
REACT_221009. Depolymerisation of the Nuclear Lamina.

Miscellaneous databases

NextBioi 20806122.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR000961. AGC-kinase_C.
IPR000008. C2_dom.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR014375. Protein_kinase_C_a/b/g.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF00168. C2. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF000550. PKC_alpha. 1 hit.
PRINTSi PR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTi SM00109. C1. 2 hits.
SM00239. C2. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
PS50004. C2. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways."
    Coussens L., Parker P.J., Rhee L., Yang-Feng T.L., Chen E., Waterfield M.D., Francke U., Ullrich A.
    Science 233:859-866(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA-II).
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA-I).
    Strain: Hereford.
    Tissue: Fetal pons.
  3. "The molecular heterogeneity of protein kinase C and its implications for cellular regulation."
    Nishizuka Y.
    Nature 334:661-665(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiKPCB_BOVIN
AccessioniPrimary (citable) accession number: P05126
Secondary accession number(s): A5D7N0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: May 18, 2010
Last modified: September 3, 2014
This is version 151 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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