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P05125 (ANF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Natriuretic peptides A
Alternative name(s):
Prepronatriodilatin

Cleaved into the following 5 chains:

  1. Atrial natriuretic factor
    Short name=ANF
    Alternative name(s):
    Atrial natriuretic peptide
    Short name=ANP
  2. Auriculin-B
  3. Auriculin-A
  4. Atriopeptin-1
    Alternative name(s):
    Atriopeptin I
  5. Atriopeptin-2
    Alternative name(s):
    Atriopeptin II
Gene names
Name:Nppa
Synonyms:Pnd
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length152 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Ref.6 Ref.8 Ref.10

Subcellular location

Secreted.

Post-translational modification

Cleaved by CORIN upon secretion to produce the functional hormone.

Disruption phenotype

Mice display increased arterial blood pressure which is dietary salt intake independent. During pregnancy, increased blood pressure is observed, leading to late gestational proteinuria and smaller litters. Impaired trophoblast invasion and smaller spiral arteries are also observed in E12.5 embryos. In E18.5 embryos, mice display fewer trophoblasts and smaller arteries in the decidua and myometrium than those in wild-type mice. Ref.6 Ref.10

Sequence similarities

Belongs to the natriuretic peptide family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHormone
Vasoactive
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcGMP biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

cardiac muscle hypertrophy in response to stress

Inferred from mutant phenotype PubMed 14985074PubMed 15452027. Source: MGI

cellular response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

female pregnancy

Inferred from mutant phenotype Ref.10. Source: UniProtKB

negative regulation of cell growth

Inferred from electronic annotation. Source: Ensembl

negative regulation of systemic arterial blood pressure

Inferred from electronic annotation. Source: Ensembl

receptor guanylyl cyclase signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of blood pressure

Inferred from mutant phenotype Ref.10. Source: UniProtKB

regulation of blood vessel size

Inferred from electronic annotation. Source: UniProtKB-KW

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to insulin

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 12738802. Source: MGI

extracellular space

Inferred from direct assay PubMed 15452027. Source: MGI

mast cell granule

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Propeptide25 – 12298 By similarity
PRO_0000001495
Peptide123 – 15028Atrial natriuretic factor By similarity
PRO_0000391784
Peptide126 – 15025Auriculin-B By similarity
PRO_0000001496
Peptide126 – 14924Auriculin-A By similarity
PRO_0000001497
Peptide127 – 14923Atriopeptin-1 By similarity
PRO_0000001498
Peptide127 – 14721Atriopeptin-2 By similarity
PRO_0000001499

Sites

Site122 – 1232Cleavage; by CORIN

Amino acid modifications

Disulfide bond129 ↔ 145 By similarity

Experimental info

Mutagenesis1221R → A: Abolishes maturation and cleavage by CORIN. Ref.7
Mutagenesis1251R → A: Does not affect maturation and cleavage by CORIN. Ref.7
Mutagenesis1261R → A: Does not affect maturation and cleavage by CORIN. Ref.7
Sequence conflict96 – 972LG → SR in AAA37235. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P05125 [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: 1693D134B5FCA23D

FASTA15216,572
        10         20         30         40         50         60 
MGSFSITLGF FLVLAFWLPG HIGANPVYSA VSNTDLMDFK NLLDHLEEKM PVEDEVMPPQ 

        70         80         90        100        110        120 
ALSEQTEEAG AALSSLPEVP PWTGEVNPPL RDGSALGRSP WDPSDRSALL KSKLRALLAG 

       130        140        150 
PRSLRRSSCF GGRIDRIGAQ SGLGCNSFRY RR 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequences of the human and mouse atrial natriuretic factor genes."
Seidman C.E., Bloch K.D., Klein K.A., Smith J.A., Seidman J.G.
Science 226:1206-1209(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Heart.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
[6]"Blood pressure and fluid-electrolyte balance in mice with reduced or absent ANP."
John S.W., Veress A.T., Honrath U., Chong C.K., Peng L., Smithies O., Sonnenberg H.
Am. J. Physiol. 271:R109-R114(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]"Processing of pro-atrial natriuretic peptide by corin in cardiac myocytes."
Wu F., Yan W., Pan J., Morser J., Wu Q.
J. Biol. Chem. 277:16900-16905(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING, MUTAGENESIS OF ARG-122; ARG-125 AND ARG-126.
[8]"Vascular natriuretic peptide receptor-linked particulate guanylate cyclases are modulated by nitric oxide-cyclic GMP signalling."
Madhani M., Scotland R.S., MacAllister R.J., Hobbs A.J.
Br. J. Pharmacol. 139:1289-1296(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Hypertension in mice lacking the proatrial natriuretic peptide convertase corin."
Chan J.C., Knudson O., Wu F., Morser J., Dole W.P., Wu Q.
Proc. Natl. Acad. Sci. U.S.A. 102:785-790(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING.
[10]"Role of corin in trophoblast invasion and uterine spiral artery remodelling in pregnancy."
Cui Y., Wang W., Dong N., Lou J., Srinivasan D.K., Cheng W., Huang X., Liu M., Fang C., Peng J., Chen S., Wu S., Liu Z., Dong L., Zhou Y., Wu Q.
Nature 484:246-250(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02781 Genomic DNA. Translation: AAA37235.1.
AK147180 mRNA. Translation: BAE27742.1.
AL714013 Genomic DNA. Translation: CAM14483.1.
CU210867 Genomic DNA. Translation: CAQ52160.1.
CH466594 Genomic DNA. Translation: EDL14792.1.
BC089615 mRNA. Translation: AAH89615.1.
CCDSCCDS18927.1.
PIRAWMS. A29370.
RefSeqNP_032751.1. NM_008725.2.
UniGeneMm.482123.

3D structure databases

ProteinModelPortalP05125.
SMRP05125. Positions 121-148.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP05125. 4 interactions.
MINTMINT-4087738.
STRING10090.ENSMUSP00000099520.

PTM databases

PhosphoSiteP05125.

Proteomic databases

MaxQBP05125.
PaxDbP05125.
PRIDEP05125.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103230; ENSMUSP00000099520; ENSMUSG00000041616.
GeneID230899.
KEGGmmu:230899.
UCSCuc008vtq.1. mouse.

Organism-specific databases

CTD4878.
MGIMGI:97367. Nppa.

Phylogenomic databases

eggNOGNOG42244.
GeneTreeENSGT00510000048659.
HOGENOMHOG000033937.
HOVERGENHBG004227.
InParanoidP05125.
KOK12334.
OMANPAQRDG.
OrthoDBEOG7K9K4Q.
TreeFamTF106304.

Gene expression databases

CleanExMM_NPPA.
GenevestigatorP05125.

Family and domain databases

InterProIPR000663. Natr_peptide.
IPR002407. Natriuretic_peptide_atrial.
[Graphical view]
PfamPF00212. ANP. 1 hit.
[Graphical view]
PRINTSPR00711. ANATPEPTIDE.
PR00710. NATPEPTIDES.
SMARTSM00183. NAT_PEP. 1 hit.
[Graphical view]
PROSITEPS00263. NATRIURETIC_PEPTIDE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio380268.
PROP05125.
SOURCESearch...

Entry information

Entry nameANF_MOUSE
AccessionPrimary (citable) accession number: P05125
Secondary accession number(s): Q5FW59
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot