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P05125

- ANF_MOUSE

UniProt

P05125 - ANF_MOUSE

Protein

Natriuretic peptides A

Gene

Nppa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.3 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei122 – 1232Cleavage; by CORIN

    GO - Biological processi

    1. cardiac muscle hypertrophy in response to stress Source: MGI
    2. cellular response to mechanical stimulus Source: Ensembl
    3. cGMP biosynthetic process Source: UniProtKB
    4. female pregnancy Source: UniProtKB
    5. negative regulation of cell growth Source: Ensembl
    6. negative regulation of systemic arterial blood pressure Source: Ensembl
    7. receptor guanylyl cyclase signaling pathway Source: UniProtKB
    8. regulation of blood pressure Source: UniProtKB
    9. regulation of blood vessel size Source: UniProtKB-KW
    10. response to hypoxia Source: Ensembl
    11. response to insulin Source: Ensembl

    Keywords - Molecular functioni

    Hormone, Vasoactive

    Enzyme and pathway databases

    ReactomeiREACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
    REACT_198563. Amyloids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Natriuretic peptides A
    Alternative name(s):
    Prepronatriodilatin
    Cleaved into the following 5 chains:
    Alternative name(s):
    Atrial natriuretic peptide
    Short name:
    ANP
    Alternative name(s):
    Atriopeptin I
    Alternative name(s):
    Atriopeptin II
    Gene namesi
    Name:Nppa
    Synonyms:Pnd
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:97367. Nppa.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. extracellular space Source: MGI
    3. mast cell granule Source: Ensembl
    4. nucleus Source: Ensembl
    5. perinuclear region of cytoplasm Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Mice display increased arterial blood pressure which is dietary salt intake independent. During pregnancy, increased blood pressure is observed, leading to late gestational proteinuria and smaller litters. Impaired trophoblast invasion and smaller spiral arteries are also observed in E12.5 embryos. In E18.5 embryos, mice display fewer trophoblasts and smaller arteries in the decidua and myometrium than those in wild-type mice.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi122 – 1221R → A: Abolishes maturation and cleavage by CORIN. 1 Publication
    Mutagenesisi125 – 1251R → A: Does not affect maturation and cleavage by CORIN. 1 Publication
    Mutagenesisi126 – 1261R → A: Does not affect maturation and cleavage by CORIN. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 12298By similarityPRO_0000001495Add
    BLAST
    Peptidei123 – 15028Atrial natriuretic factorBy similarityPRO_0000391784Add
    BLAST
    Peptidei126 – 15025Auriculin-BBy similarityPRO_0000001496Add
    BLAST
    Peptidei126 – 14924Auriculin-ABy similarityPRO_0000001497Add
    BLAST
    Peptidei127 – 14923Atriopeptin-1By similarityPRO_0000001498Add
    BLAST
    Peptidei127 – 14721Atriopeptin-2By similarityPRO_0000001499Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi129 ↔ 145By similarity

    Post-translational modificationi

    Cleaved by CORIN upon secretion to produce the functional hormone.

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    MaxQBiP05125.
    PaxDbiP05125.
    PRIDEiP05125.

    PTM databases

    PhosphoSiteiP05125.

    Expressioni

    Gene expression databases

    CleanExiMM_NPPA.
    GenevestigatoriP05125.

    Interactioni

    Protein-protein interaction databases

    IntActiP05125. 4 interactions.
    MINTiMINT-4087738.
    STRINGi10090.ENSMUSP00000099520.

    Structurei

    3D structure databases

    ProteinModelPortaliP05125.
    SMRiP05125. Positions 121-148.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the natriuretic peptide family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG42244.
    GeneTreeiENSGT00510000048659.
    HOGENOMiHOG000033937.
    HOVERGENiHBG004227.
    InParanoidiP05125.
    KOiK12334.
    OMAiNPAQRDG.
    OrthoDBiEOG7K9K4Q.
    TreeFamiTF106304.

    Family and domain databases

    InterProiIPR000663. Natr_peptide.
    IPR002407. Natriuretic_peptide_atrial.
    [Graphical view]
    PfamiPF00212. ANP. 1 hit.
    [Graphical view]
    PRINTSiPR00711. ANATPEPTIDE.
    PR00710. NATPEPTIDES.
    SMARTiSM00183. NAT_PEP. 1 hit.
    [Graphical view]
    PROSITEiPS00263. NATRIURETIC_PEPTIDE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05125-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSFSITLGF FLVLAFWLPG HIGANPVYSA VSNTDLMDFK NLLDHLEEKM    50
    PVEDEVMPPQ ALSEQTEEAG AALSSLPEVP PWTGEVNPPL RDGSALGRSP 100
    WDPSDRSALL KSKLRALLAG PRSLRRSSCF GGRIDRIGAQ SGLGCNSFRY 150
    RR 152
    Length:152
    Mass (Da):16,572
    Last modified:October 3, 2012 - v2
    Checksum:i1693D134B5FCA23D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti96 – 972LG → SR in AAA37235. (PubMed:6542248)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02781 Genomic DNA. Translation: AAA37235.1.
    AK147180 mRNA. Translation: BAE27742.1.
    AL714013 Genomic DNA. Translation: CAM14483.1.
    CU210867 Genomic DNA. Translation: CAQ52160.1.
    CH466594 Genomic DNA. Translation: EDL14792.1.
    BC089615 mRNA. Translation: AAH89615.1.
    CCDSiCCDS18927.1.
    PIRiA29370. AWMS.
    RefSeqiNP_032751.1. NM_008725.2.
    UniGeneiMm.482123.

    Genome annotation databases

    EnsembliENSMUST00000103230; ENSMUSP00000099520; ENSMUSG00000041616.
    GeneIDi230899.
    KEGGimmu:230899.
    UCSCiuc008vtq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02781 Genomic DNA. Translation: AAA37235.1 .
    AK147180 mRNA. Translation: BAE27742.1 .
    AL714013 Genomic DNA. Translation: CAM14483.1 .
    CU210867 Genomic DNA. Translation: CAQ52160.1 .
    CH466594 Genomic DNA. Translation: EDL14792.1 .
    BC089615 mRNA. Translation: AAH89615.1 .
    CCDSi CCDS18927.1.
    PIRi A29370. AWMS.
    RefSeqi NP_032751.1. NM_008725.2.
    UniGenei Mm.482123.

    3D structure databases

    ProteinModelPortali P05125.
    SMRi P05125. Positions 121-148.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P05125. 4 interactions.
    MINTi MINT-4087738.
    STRINGi 10090.ENSMUSP00000099520.

    PTM databases

    PhosphoSitei P05125.

    Proteomic databases

    MaxQBi P05125.
    PaxDbi P05125.
    PRIDEi P05125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103230 ; ENSMUSP00000099520 ; ENSMUSG00000041616 .
    GeneIDi 230899.
    KEGGi mmu:230899.
    UCSCi uc008vtq.1. mouse.

    Organism-specific databases

    CTDi 4878.
    MGIi MGI:97367. Nppa.

    Phylogenomic databases

    eggNOGi NOG42244.
    GeneTreei ENSGT00510000048659.
    HOGENOMi HOG000033937.
    HOVERGENi HBG004227.
    InParanoidi P05125.
    KOi K12334.
    OMAi NPAQRDG.
    OrthoDBi EOG7K9K4Q.
    TreeFami TF106304.

    Enzyme and pathway databases

    Reactomei REACT_196516. YAP1- and WWTR1 (TAZ)-stimulated gene expression.
    REACT_198563. Amyloids.

    Miscellaneous databases

    NextBioi 380268.
    PROi P05125.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_NPPA.
    Genevestigatori P05125.

    Family and domain databases

    InterProi IPR000663. Natr_peptide.
    IPR002407. Natriuretic_peptide_atrial.
    [Graphical view ]
    Pfami PF00212. ANP. 1 hit.
    [Graphical view ]
    PRINTSi PR00711. ANATPEPTIDE.
    PR00710. NATPEPTIDES.
    SMARTi SM00183. NAT_PEP. 1 hit.
    [Graphical view ]
    PROSITEi PS00263. NATRIURETIC_PEPTIDE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences of the human and mouse atrial natriuretic factor genes."
      Seidman C.E., Bloch K.D., Klein K.A., Smith J.A., Seidman J.G.
      Science 226:1206-1209(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Heart.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.
    6. "Blood pressure and fluid-electrolyte balance in mice with reduced or absent ANP."
      John S.W., Veress A.T., Honrath U., Chong C.K., Peng L., Smithies O., Sonnenberg H.
      Am. J. Physiol. 271:R109-R114(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    7. "Processing of pro-atrial natriuretic peptide by corin in cardiac myocytes."
      Wu F., Yan W., Pan J., Morser J., Wu Q.
      J. Biol. Chem. 277:16900-16905(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING, MUTAGENESIS OF ARG-122; ARG-125 AND ARG-126.
    8. "Vascular natriuretic peptide receptor-linked particulate guanylate cyclases are modulated by nitric oxide-cyclic GMP signalling."
      Madhani M., Scotland R.S., MacAllister R.J., Hobbs A.J.
      Br. J. Pharmacol. 139:1289-1296(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Hypertension in mice lacking the proatrial natriuretic peptide convertase corin."
      Chan J.C., Knudson O., Wu F., Morser J., Dole W.P., Wu Q.
      Proc. Natl. Acad. Sci. U.S.A. 102:785-790(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING.
    10. "Role of corin in trophoblast invasion and uterine spiral artery remodelling in pregnancy."
      Cui Y., Wang W., Dong N., Lou J., Srinivasan D.K., Cheng W., Huang X., Liu M., Fang C., Peng J., Chen S., Wu S., Liu Z., Dong L., Zhou Y., Wu Q.
      Nature 484:246-250(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiANF_MOUSE
    AccessioniPrimary (citable) accession number: P05125
    Secondary accession number(s): Q5FW59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3