P05125 (ANF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Natriuretic peptides A

Alternative name(s):
Prepronatriodilatin

Cleaved into the following 5 chains:

1. Atrial natriuretic factor
   Short name=ANF
   Alternative name(s):
   Atrial natriuretic peptide
   Short name=ANP
2. Auriculin-B
3. Auriculin-A
4. Atriopeptin-1
   Alternative name(s):
   Atriopeptin I
5. Atriopeptin-2
   Alternative name(s):
   Atriopeptin II

Gene names

Name:	Nppa
Synonyms:	Pnd

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Ref.6 Ref.8 Ref.10
Subcellular location	Secreted.
Post-translational modification	Cleaved by CORIN upon secretion to produce the functional hormone.
Disruption phenotype	Mice display increased arterial blood pressure which is dietary salt intake independent. During pregnancy, increased blood pressure is observed, leading to late gestational proteinuria and smaller litters. Impaired trophoblast invasion and smaller spiral arteries are also observed in E12.5 embryos. In E18.5 embryos, mice display fewer trophoblasts and smaller arteries in the decidua and myometrium than those in wild-type mice. Ref.6 Ref.10
Sequence similarities	Belongs to the natriuretic peptide family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone Vasoactive
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cGMP biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB cardiac muscle hypertrophy in response to stress Inferred from mutant phenotype PubMed 14985074PubMed 15452027. Source: MGI female pregnancy Inferred from mutant phenotype Ref.10. Source: UniProtKB negative regulation of cell growth Inferred from electronic annotation. Source: Compara receptor guanylyl cyclase signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB regulation of blood pressure Inferred from mutant phenotype Ref.10. Source: UniProtKB regulation of blood vessel size Inferred from electronic annotation. Source: UniProtKB-KW response to insulin stimulus Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from direct assay PubMed 12738802. Source: MGI extracellular space Inferred from direct assay PubMed 15452027. Source: MGI mast cell granule Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 122	98	By similarity		PRO_0000001495
	Peptide	123 – 150	28	Atrial natriuretic factor By similarity		PRO_0000391784
	Peptide	126 – 150	25	Auriculin-B By similarity		PRO_0000001496
	Peptide	126 – 149	24	Auriculin-A By similarity		PRO_0000001497
	Peptide	127 – 149	23	Atriopeptin-1 By similarity		PRO_0000001498
	Peptide	127 – 147	21	Atriopeptin-2 By similarity		PRO_0000001499
Sites
	Site	122 – 123	2	Cleavage; by CORIN
Amino acid modifications
	Disulfide bond	129 ↔ 145		By similarity
Experimental info
	Mutagenesis	122	1	R → A: Abolishes maturation and cleavage by CORIN. Ref.7
	Mutagenesis	125	1	R → A: Does not affect maturation and cleavage by CORIN. Ref.7
	Mutagenesis	126	1	R → A: Does not affect maturation and cleavage by CORIN. Ref.7
	Sequence conflict	96 – 97	2	LG → SR in AAA37235. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P05125 [UniParc]. Last modified October 3, 2012. Version 2. Checksum: 1693D134B5FCA23D	FASTA	152	16,572
	10 20 30 40 50 60 MGSFSITLGF FLVLAFWLPG HIGANPVYSA VSNTDLMDFK NLLDHLEEKM PVEDEVMPPQ 70 80 90 100 110 120 ALSEQTEEAG AALSSLPEVP PWTGEVNPPL RDGSALGRSP WDPSDRSALL KSKLRALLAG 130 140 150 PRSLRRSSCF GGRIDRIGAQ SGLGCNSFRY RR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequences of the human and mouse atrial natriuretic factor genes." Seidman C.E., Bloch K.D., Klein K.A., Smith J.A., Seidman J.G. Science 226:1206-1209(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Heart.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[6]	"Blood pressure and fluid-electrolyte balance in mice with reduced or absent ANP." John S.W., Veress A.T., Honrath U., Chong C.K., Peng L., Smithies O., Sonnenberg H. Am. J. Physiol. 271:R109-R114(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Processing of pro-atrial natriuretic peptide by corin in cardiac myocytes." Wu F., Yan W., Pan J., Morser J., Wu Q. J. Biol. Chem. 277:16900-16905(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING, MUTAGENESIS OF ARG-122; ARG-125 AND ARG-126.
[8]	"Vascular natriuretic peptide receptor-linked particulate guanylate cyclases are modulated by nitric oxide-cyclic GMP signalling." Madhani M., Scotland R.S., MacAllister R.J., Hobbs A.J. Br. J. Pharmacol. 139:1289-1296(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Hypertension in mice lacking the proatrial natriuretic peptide convertase corin." Chan J.C., Knudson O., Wu F., Morser J., Dole W.P., Wu Q. Proc. Natl. Acad. Sci. U.S.A. 102:785-790(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING.
[10]	"Role of corin in trophoblast invasion and uterine spiral artery remodelling in pregnancy." Cui Y., Wang W., Dong N., Lou J., Srinivasan D.K., Cheng W., Huang X., Liu M., Fang C., Peng J., Chen S., Wu S., Liu Z., Dong L., Zhou Y., Wu Q. Nature 484:246-250(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02781 Genomic DNA. Translation: AAA37235.1. AK147180 mRNA. Translation: BAE27742.1. AL714013 Genomic DNA. Translation: CAM14483.1. CU210867 Genomic DNA. Translation: CAQ52160.1. CH466594 Genomic DNA. Translation: EDL14792.1. BC089615 mRNA. Translation: AAH89615.1.
IPI	IPI00116489.
PIR	AWMS. A29370.
RefSeq	NP_032751.1. NM_008725.2.
UniGene	Mm.482123.
3D structure databases
ProteinModelPortal	P05125.
SMR	P05125. Positions 121-148.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05125. 3 interactions.
STRING	10090.ENSMUSP00000099520.
PTM databases
PhosphoSite	P05125.
Proteomic databases
PaxDb	P05125.
PRIDE	P05125.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000103230; ENSMUSP00000099520; ENSMUSG00000041616.
GeneID	230899.
KEGG	mmu:230899.
Organism-specific databases
CTD	4878.
MGI	MGI:97367. Nppa.
Phylogenomic databases
eggNOG	NOG42244.
GeneTree	ENSGT00510000048659.
HOGENOM	HOG000033937.
HOVERGEN	HBG004227.
InParanoid	P05125.
KO	K12334.
OMA	EVPPWTG.
OrthoDB	EOG40K81J.
Gene expression databases
CleanEx	MM_NPPA.
Genevestigator	P05125.
GermOnline	ENSMUSG00000041616. Mus musculus.
Family and domain databases
InterPro	IPR000663. Natr_peptide. IPR002407. Natriuretic_peptide_atrial. [Graphical view]
Pfam	PF00212. ANP. 1 hit. [Graphical view]
PRINTS	PR00711. ANATPEPTIDE. PR00710. NATPEPTIDES.
SMART	SM00183. NAT_PEP. 1 hit. [Graphical view]
PROSITE	PS00263. NATRIURETIC_PEPTIDE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	380268.
SOURCE	Search...

Entry information

Entry name

ANF_MOUSE

Accession

Primary (citable) accession number: P05125
Secondary accession number(s): Q5FW59

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program