P05125 (ANF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Natriuretic peptides A

Alternative name(s):
Prepronatriodilatin

Cleaved into the following 5 chains:

1. Atrial natriuretic factor
   Short name=ANF
   Alternative name(s):
   Atrial natriuretic peptide
   Short name=ANP
2. Auriculin-B
3. Auriculin-A
4. Atriopeptin-1
   Alternative name(s):
   Atriopeptin I
5. Atriopeptin-2
   Alternative name(s):
   Atriopeptin II

Gene names

Name:	Nppa
Synonyms:	Pnd

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilatation. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Ref.2 Ref.3
Subcellular location	Secreted.
Post-translational modification	Cleaved by CORIN upon secretion to produce the functional hormone.
Disruption phenotype	Mice display increased arterial blood pressure which is dietary salt intake independent. Ref.2
Sequence similarities	Belongs to the natriuretic peptide family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone Vasoactive
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cGMP biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB receptor guanylyl cyclase signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB regulation of blood vessel size Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 122	98	By similarity		PRO_0000001495
	Peptide	123 – 150	28	Atrial natriuretic factor By similarity		PRO_0000391784
	Peptide	126 – 150	25	Auriculin-B By similarity		PRO_0000001496
	Peptide	126 – 149	24	Auriculin-A By similarity		PRO_0000001497
	Peptide	127 – 149	23	Atriopeptin-1 By similarity		PRO_0000001498
	Peptide	127 – 147	21	Atriopeptin-2 By similarity		PRO_0000001499
Sites
	Site	122 – 123	2	Cleavage; by CORIN By similarity
Amino acid modifications
	Disulfide bond	129 ↔ 145		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05125 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: FC8CC43EAAFCA227	FASTA	152	16,645
	10 20 30 40 50 60 MGSFSITLGF FLVLAFWLPG HIGANPVYSA VSNTDLMDFK NLLDHLEEKM PVEDEVMPPQ 70 80 90 100 110 120 ALSEQTEEAG AALSSLPEVP PWTGEVNPPL RDGSASRRSP WDPSDRSALL KSKLRALLAG 130 140 150 PRSLRRSSCF GGRIDRIGAQ SGLGCNSFRY RR

References

[1]	"Nucleotide sequences of the human and mouse atrial natriuretic factor genes." Seidman C.E., Bloch K.D., Klein K.A., Smith J.A., Seidman J.G. Science 226:1206-1209(1984) [PubMed: 6542248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Blood pressure and fluid-electrolyte balance in mice with reduced or absent ANP." John S.W., Veress A.T., Honrath U., Chong C.K., Peng L., Smithies O., Sonnenberg H. Am. J. Physiol. 271:R109-R114(1996) [PubMed: 8760210] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[3]	"Vascular natriuretic peptide receptor-linked particulate guanylate cyclases are modulated by nitric oxide-cyclic GMP signalling." Madhani M., Scotland R.S., MacAllister R.J., Hobbs A.J. Br. J. Pharmacol. 139:1289-1296(2003) [PubMed: 12890708] [Abstract] Cited for: FUNCTION.
[4]	"Hypertension in mice lacking the proatrial natriuretic peptide convertase corin." Chan J.C., Knudson O., Wu F., Morser J., Dole W.P., Wu Q. Proc. Natl. Acad. Sci. U.S.A. 102:785-790(2005) [PubMed: 15637153] [Abstract] Cited for: PROCESSING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02781 Genomic DNA. Translation: AAA37235.1.
IPI	IPI00116489.
PIR	AWMS. A29370.
UniGene	Mm.482123.
3D structure databases
ProteinModelPortal	P05125.
SMR	P05125. Positions 125-151.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05125. 3 interactions.
STRING	P05125.
Proteomic databases
PRIDE	P05125.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:97367. Nppa.
Phylogenomic databases
HOGENOM	HBG283050.
HOVERGEN	HBG004227.
InParanoid	P05125.
OrthoDB	EOG40K81J.
Gene expression databases
ArrayExpress	P05125.
Bgee	P05125.
CleanEx	MM_NPPA.
Genevestigator	P05125.
GermOnline	ENSMUSG00000041616. Mus musculus.
Family and domain databases
InterPro	IPR000663. Natr_peptide. IPR002407. Natriuretic_peptide_atrial. [Graphical view]
Pfam	PF00212. ANP. 1 hit. [Graphical view]
PRINTS	PR00711. ANATPEPTIDE. PR00710. NATPEPTIDES.
SMART	SM00183. NAT_PEP. 1 hit. [Graphical view]
PROSITE	PS00263. NATRIURETIC_PEPTIDE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

ANF_MOUSE

Accession

Primary (citable) accession number: P05125

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	September 21, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program