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P05124 (KCRB_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Creatine kinase B-type

EC=2.7.3.2
Alternative name(s):
B-CK
Creatine kinase B chain
Gene names
Name:CKB
OrganismCanis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity

ATP + creatine = ADP + phosphocreatine.

Subunit structure

Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the ATP:guanido phosphotransferase family.

Contains 1 phosphagen kinase C-terminal domain.

Contains 1 phosphagen kinase N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 381380Creatine kinase B-type
PRO_0000211965

Regions

Domain11 – 9888Phosphagen kinase N-terminal
Domain125 – 367243Phosphagen kinase C-terminal
Nucleotide binding128 – 1325ATP By similarity
Nucleotide binding320 – 3256ATP By similarity

Sites

Binding site1301ATP By similarity
Binding site1321ATP By similarity
Binding site1911ATP By similarity
Binding site2321Substrate By similarity
Binding site2361ATP By similarity
Binding site2851Substrate By similarity
Binding site2921ATP By similarity
Binding site3201ATP By similarity
Binding site3351ATP By similarity

Amino acid modifications

Modified residue41Phosphoserine By similarity
Modified residue351Phosphothreonine By similarity
Modified residue1251Phosphotyrosine By similarity
Modified residue1991Phosphoserine By similarity
Modified residue2691Nitrated tyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
P05124 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 2C2F925C78F16364

FASTA38142,701
        10         20         30         40         50         60 
MPFSNSHNTL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG 

        70         80         90        100        110        120 
VDNPGHPYIM TVGCVAGDEE SYDVFKELFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD 

       130        140        150        160        170        180 
LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT 

       190        200        210        220        230        240 
EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM 

       250        260        270        280        290        300 
QKGGNMKEVF TRFCNGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP 

       310        320        330        340        350        360 
HLGKHEKFPE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL 

       370        380 
IEMEQRLEQG QAIDDLVPAQ K 

« Hide

References

[1]"The complete nucleotide sequence of canine brain B creatine kinase mRNA: homology in the coding and 3' noncoding regions among species."
Billadello J.J., Kelly D.P., Roman D.G., Strauss A.W.
Biochem. Biophys. Res. Commun. 138:392-398(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Complete nucleotide sequence of dog heart creatine kinase mRNA: conservation of amino acid sequence within and among species."
Roman D.G., Billadello J.J., Gordon J., Grace A., Sobel B., Strauss A.W.
Proc. Natl. Acad. Sci. U.S.A. 82:8394-8398(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-30; 44-61; 87-95; 138-149; 158-171; 178-208; 224-236; 247-261; 268-287; 321-357 AND 364-381.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13453 mRNA. Translation: AAA30837.1.
PIRB24686. A24227.
RefSeqXP_003639259.1. XM_003639211.2.

3D structure databases

ProteinModelPortalP05124.
SMRP05124. Positions 2-381.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9615.ENSCAFP00000026998.

Proteomic databases

PaxDbP05124.
PRIDEP05124.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSCAFT00000029035; ENSCAFP00000026998; ENSCAFG00000018277.
GeneID100855552.
KEGGcfa:100855552.

Organism-specific databases

CTD1152.

Phylogenomic databases

eggNOGCOG3869.
GeneTreeENSGT00550000074561.
HOGENOMHOG000232165.
HOVERGENHBG001339.
InParanoidP05124.
KOK00933.
OMARGHEFMW.
OrthoDBEOG7XM2XW.
TreeFamTF314214.

Family and domain databases

Gene3D1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMSSF48034. SSF48034. 1 hit.
PROSITEPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20855457.

Entry information

Entry nameKCRB_CANFA
AccessionPrimary (citable) accession number: P05124
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: March 19, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families