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P05124

- KCRB_CANFA

UniProt

P05124 - KCRB_CANFA

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Protein
Creatine kinase B-type
Gene
CKB
Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei130 – 1301ATP By similarity
Binding sitei132 – 1321ATP By similarity
Binding sitei191 – 1911ATP By similarity
Binding sitei232 – 2321Substrate By similarity
Binding sitei236 – 2361ATP By similarity
Binding sitei285 – 2851Substrate By similarity
Binding sitei292 – 2921ATP By similarity
Binding sitei320 – 3201ATP By similarity
Binding sitei335 – 3351ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi128 – 1325ATP By similarity
Nucleotide bindingi320 – 3256ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. creatine kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. brain development Source: AgBase
  2. cellular chloride ion homeostasis Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase B-type (EC:2.7.3.2)
Alternative name(s):
B-CK
Creatine kinase B chain
Gene namesi
Name:CKB
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Chromosome 8

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 381380Creatine kinase B-type
PRO_0000211965Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41Phosphoserine By similarity
Modified residuei35 – 351Phosphothreonine By similarity
Modified residuei125 – 1251Phosphotyrosine By similarity
Modified residuei199 – 1991Phosphoserine By similarity
Modified residuei269 – 2691Nitrated tyrosine By similarity

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

PaxDbiP05124.
PRIDEiP05124.

Interactioni

Subunit structurei

Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000026998.

Structurei

3D structure databases

ProteinModelPortaliP05124.
SMRiP05124. Positions 2-381.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 9888Phosphagen kinase N-terminal
Add
BLAST
Domaini125 – 367243Phosphagen kinase C-terminal
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3869.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP05124.
KOiK00933.
OMAiQCLSDVR.
OrthoDBiEOG7XM2XW.
TreeFamiTF314214.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05124-1 [UniParc]FASTAAdd to Basket

« Hide

MPFSNSHNTL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG    50
FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYDVFKELFD PIIEDRHGGY 100
KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE 150
RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP 200
LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF 250
TRFCNGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP 300
HLGKHEKFPE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV 350
QMVVDGVKLL IEMEQRLEQG QAIDDLVPAQ K 381
Length:381
Mass (Da):42,701
Last modified:August 13, 1987 - v1
Checksum:i2C2F925C78F16364
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13453 mRNA. Translation: AAA30837.1.
PIRiA24227. B24686.
RefSeqiXP_003639259.1. XM_003639211.2.

Genome annotation databases

EnsembliENSCAFT00000029035; ENSCAFP00000026998; ENSCAFG00000018277.
GeneIDi100855552.
KEGGicfa:100855552.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M13453 mRNA. Translation: AAA30837.1 .
PIRi A24227. B24686.
RefSeqi XP_003639259.1. XM_003639211.2.

3D structure databases

ProteinModelPortali P05124.
SMRi P05124. Positions 2-381.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9615.ENSCAFP00000026998.

Proteomic databases

PaxDbi P05124.
PRIDEi P05124.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSCAFT00000029035 ; ENSCAFP00000026998 ; ENSCAFG00000018277 .
GeneIDi 100855552.
KEGGi cfa:100855552.

Organism-specific databases

CTDi 1152.

Phylogenomic databases

eggNOGi COG3869.
GeneTreei ENSGT00550000074561.
HOGENOMi HOG000232165.
HOVERGENi HBG001339.
InParanoidi P05124.
KOi K00933.
OMAi QCLSDVR.
OrthoDBi EOG7XM2XW.
TreeFami TF314214.

Miscellaneous databases

NextBioi 20855457.

Family and domain databases

Gene3Di 1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view ]
Pfami PF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view ]
SUPFAMi SSF48034. SSF48034. 1 hit.
PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete nucleotide sequence of canine brain B creatine kinase mRNA: homology in the coding and 3' noncoding regions among species."
    Billadello J.J., Kelly D.P., Roman D.G., Strauss A.W.
    Biochem. Biophys. Res. Commun. 138:392-398(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Complete nucleotide sequence of dog heart creatine kinase mRNA: conservation of amino acid sequence within and among species."
    Roman D.G., Billadello J.J., Gordon J., Grace A., Sobel B., Strauss A.W.
    Proc. Natl. Acad. Sci. U.S.A. 82:8394-8398(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-30; 44-61; 87-95; 138-149; 158-171; 178-208; 224-236; 247-261; 268-287; 321-357 AND 364-381.
    Tissue: Brain.

Entry informationi

Entry nameiKCRB_CANFA
AccessioniPrimary (citable) accession number: P05124
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: May 14, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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