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Protein

Creatine kinase B-type

Gene

CKB

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei130ATPPROSITE-ProRule annotation1
Binding sitei132ATPPROSITE-ProRule annotation1
Binding sitei191ATPPROSITE-ProRule annotation1
Binding sitei232SubstrateBy similarity1
Binding sitei236ATPPROSITE-ProRule annotation1
Binding sitei285SubstrateBy similarity1
Binding sitei292ATPPROSITE-ProRule annotation1
Binding sitei320ATPPROSITE-ProRule annotation1
Binding sitei335ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 132ATPPROSITE-ProRule annotation5
Nucleotide bindingi320 – 325ATPPROSITE-ProRule annotation6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • creatine kinase activity Source: AgBase

GO - Biological processi

  • ATP biosynthetic process Source: AgBase
  • substantia nigra development Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase B-type (EC:2.7.3.2)
Alternative name(s):
B-CK
Creatine kinase B chain
Gene namesi
Name:CKB
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: AgBase
  • extracellular exosome Source: Ensembl
  • extracellular membrane-bounded organelle Source: AgBase
  • extracellular space Source: AgBase
  • mitochondrion Source: Ensembl
  • myelin sheath Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002119711 – 381Creatine kinase B-typeAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei282Phosphothreonine; by autocatalysis1 Publication1
Modified residuei285Phosphoserine; by autocatalysis1 Publication1
Modified residuei289Phosphothreonine; by autocatalysis1 Publication1

Post-translational modificationi

Ba-CK and Bb-CK are phosphorylated.1 Publication
The N-terminus of BA-CK is blocked.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP05122.
PRIDEiP05122.

PTM databases

iPTMnetiP05122.

Expressioni

Tissue specificityi

Expressed in almost all tissues and found enriched in various region of the brain, retina heart, gizzard, gut and sperm.

Interactioni

Subunit structurei

Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Protein-protein interaction databases

BioGridi676508. 1 interactor.
IntActiP05122. 1 interactor.
STRINGi9031.ENSGALP00000018743.

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 13Combined sources8
Helixi16 – 19Combined sources4
Helixi29 – 33Combined sources5
Helixi36 – 42Combined sources7
Helixi53 – 62Combined sources10
Beta strandi67 – 69Combined sources3
Helixi81 – 84Combined sources4
Helixi86 – 96Combined sources11
Helixi112 – 114Combined sources3
Turni123 – 125Combined sources3
Beta strandi126 – 135Combined sources10
Turni143 – 145Combined sources3
Helixi148 – 162Combined sources15
Helixi167 – 169Combined sources3
Beta strandi171 – 175Combined sources5
Helixi176 – 178Combined sources3
Helixi181 – 189Combined sources9
Helixi200 – 203Combined sources4
Turni204 – 214Combined sources11
Beta strandi216 – 220Combined sources5
Beta strandi223 – 244Combined sources22
Helixi246 – 266Combined sources21
Turni275 – 277Combined sources3
Helixi284 – 286Combined sources3
Beta strandi292 – 298Combined sources7
Helixi300 – 303Combined sources4
Helixi308 – 315Combined sources8
Beta strandi317 – 320Combined sources4
Turni329 – 332Combined sources4
Beta strandi333 – 338Combined sources6
Beta strandi342 – 344Combined sources3
Helixi346 – 368Combined sources23
Helixi374 – 376Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QH4X-ray1.41A/B/C/D2-381[»]
ProteinModelPortaliP05122.
SMRiP05122.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05122.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 98Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini125 – 367Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP05122.
KOiK00933.
PhylomeDBiP05122.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Bb-CK-1 (identifier: P05122-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPFSNSHNLL KMKYSVDDEY PDLSVHNNHM AKVLTLDLYK KLRDRQTSSG
60 70 80 90 100
FTLDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PVIEDRHGGY
110 120 130 140 150
KPTDEHKTDL NADNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLSVE ALGSLGGDLK GKYYALRNMT DAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
NLGKHEKFGE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEKRLEKG QSIDDLMPAQ K
Length:381
Mass (Da):42,871
Last modified:August 13, 1987 - v1
Checksum:i313BCCB46BCDD02B
GO
Isoform Ba-CK (identifier: P05122-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-65: MPFSNSHNLL...VIQTGVDNPG → MAQLNNQRLP...VIQTGVDNPG

Show »
Length:376
Mass (Da):42,267
Checksum:i5A107A22ABCB046F
GO
Isoform Bb-CK-2 (identifier: P05122-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Show »
Length:370
Mass (Da):41,601
Checksum:iA08833EFEBB99797
GO
Isoform Bb-CK-3 (identifier: P05122-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Show »
Length:352
Mass (Da):39,456
Checksum:iD0BE655ACDE7C082
GO
Isoform Bb-CK-4 (identifier: P05122-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: Missing.

Show »
Length:312
Mass (Da):34,983
Checksum:i0FDE036FF4AD60C6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0107701 – 69Missing in isoform Bb-CK-4. CuratedAdd BLAST69
Alternative sequenceiVSP_0028141 – 65MPFSN…VDNPG → MAQLNNQRLPPEEEYPDLST HNNHMAKVLTLDLYKKLRDR VTPSGFTLDDVIQTGVDNPG in isoform Ba-CK. CuratedAdd BLAST65
Alternative sequenceiVSP_0107691 – 29Missing in isoform Bb-CK-3. CuratedAdd BLAST29
Alternative sequenceiVSP_0107681 – 11Missing in isoform Bb-CK-2. CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03509 mRNA. Translation: CAA27221.1.
M33714
, M33711, M33712, M33713 Genomic DNA. Translation: AAA48613.1.
M33714
, M33711, M33712, M33713 Genomic DNA. Translation: AAA48614.1.
M35381 Genomic DNA. Translation: AAA48687.1.
PIRiA37059. A24793.
RefSeqiNP_990641.1. NM_205310.1. [P05122-1]
XP_015142790.1. XM_015287304.1. [P05122-2]
UniGeneiGga.2722.

Genome annotation databases

EnsembliENSGALT00000018765; ENSGALP00000018742; ENSGALG00000011511. [P05122-1]
ENSGALT00000053131; ENSGALP00000047068; ENSGALG00000011511. [P05122-2]
GeneIDi396248.
KEGGigga:396248.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03509 mRNA. Translation: CAA27221.1.
M33714
, M33711, M33712, M33713 Genomic DNA. Translation: AAA48613.1.
M33714
, M33711, M33712, M33713 Genomic DNA. Translation: AAA48614.1.
M35381 Genomic DNA. Translation: AAA48687.1.
PIRiA37059. A24793.
RefSeqiNP_990641.1. NM_205310.1. [P05122-1]
XP_015142790.1. XM_015287304.1. [P05122-2]
UniGeneiGga.2722.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QH4X-ray1.41A/B/C/D2-381[»]
ProteinModelPortaliP05122.
SMRiP05122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676508. 1 interactor.
IntActiP05122. 1 interactor.
STRINGi9031.ENSGALP00000018743.

PTM databases

iPTMnetiP05122.

Proteomic databases

PaxDbiP05122.
PRIDEiP05122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000018765; ENSGALP00000018742; ENSGALG00000011511. [P05122-1]
ENSGALT00000053131; ENSGALP00000047068; ENSGALG00000011511. [P05122-2]
GeneIDi396248.
KEGGigga:396248.

Organism-specific databases

CTDi1152.

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP05122.
KOiK00933.
PhylomeDBiP05122.

Miscellaneous databases

EvolutionaryTraceiP05122.
PROiP05122.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCRB_CHICK
AccessioniPrimary (citable) accession number: P05122
Secondary accession number(s): Q92061
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.