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P05122

- KCRB_CHICK

UniProt

P05122 - KCRB_CHICK

Protein

Creatine kinase B-type

Gene

CKB

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

    Catalytic activityi

    ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei130 – 1301ATPPROSITE-ProRule annotation
    Binding sitei132 – 1321ATPPROSITE-ProRule annotation
    Binding sitei191 – 1911ATPPROSITE-ProRule annotation
    Binding sitei232 – 2321SubstrateBy similarity
    Binding sitei236 – 2361ATPPROSITE-ProRule annotation
    Binding sitei285 – 2851SubstrateBy similarity
    Binding sitei292 – 2921ATPPROSITE-ProRule annotation
    Binding sitei320 – 3201ATPPROSITE-ProRule annotation
    Binding sitei335 – 3351ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi128 – 1325ATPPROSITE-ProRule annotation
    Nucleotide bindingi320 – 3256ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. creatine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Creatine kinase B-type (EC:2.7.3.2)
    Alternative name(s):
    B-CK
    Creatine kinase B chain
    Gene namesi
    Name:CKB
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 381381Creatine kinase B-typePRO_0000211971Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei282 – 2821Phosphothreonine; by autocatalysis1 Publication
    Modified residuei285 – 2851Phosphoserine; by autocatalysis1 Publication
    Modified residuei289 – 2891Phosphothreonine; by autocatalysis1 Publication

    Post-translational modificationi

    Ba-CK and Bb-CK are phosphorylated.1 Publication
    The N-terminus of BA-CK is blocked.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP05122.
    PRIDEiP05122.

    Expressioni

    Tissue specificityi

    Expressed in almost all tissues and found enriched in various region of the brain, retina heart, gizzard, gut and sperm.

    Interactioni

    Subunit structurei

    Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

    Protein-protein interaction databases

    BioGridi676508. 1 interaction.
    IntActiP05122. 1 interaction.

    Structurei

    Secondary structure

    1
    381
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 138
    Helixi16 – 194
    Helixi29 – 335
    Helixi36 – 427
    Helixi53 – 6210
    Beta strandi67 – 693
    Helixi81 – 844
    Helixi86 – 9611
    Helixi112 – 1143
    Turni123 – 1253
    Beta strandi126 – 13510
    Turni143 – 1453
    Helixi148 – 16215
    Helixi167 – 1693
    Beta strandi171 – 1755
    Helixi176 – 1783
    Helixi181 – 1899
    Helixi200 – 2034
    Turni204 – 21411
    Beta strandi216 – 2205
    Beta strandi223 – 24422
    Helixi246 – 26621
    Turni275 – 2773
    Helixi284 – 2863
    Beta strandi292 – 2987
    Helixi300 – 3034
    Helixi308 – 3158
    Beta strandi317 – 3204
    Turni329 – 3324
    Beta strandi333 – 3386
    Beta strandi342 – 3443
    Helixi346 – 36823
    Helixi374 – 3763

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1QH4X-ray1.41A/B/C/D2-381[»]
    ProteinModelPortaliP05122.
    SMRiP05122. Positions 2-381.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05122.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 9888Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini125 – 367243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG3869.
    HOGENOMiHOG000232165.
    HOVERGENiHBG001339.
    KOiK00933.
    PhylomeDBiP05122.

    Family and domain databases

    Gene3Di1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 1 hit.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view]

    Sequences (5)i

    Sequence statusi: Complete.

    This entry describes 5 isoformsi produced by alternative splicing. Align

    Isoform Bb-CK-1 (identifier: P05122-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPFSNSHNLL KMKYSVDDEY PDLSVHNNHM AKVLTLDLYK KLRDRQTSSG    50
    FTLDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PVIEDRHGGY 100
    KPTDEHKTDL NADNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE 150
    RRAIEKLSVE ALGSLGGDLK GKYYALRNMT DAEQQQLIDD HFLFDKPVSP 200
    LLLASGMARD WPDARGIWHN DNKTFLVWIN EEDHLRVISM QKGGNMKEVF 250
    TRFCTGLTQI ETLFKSKNYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP 300
    NLGKHEKFGE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV 350
    QMVVDGVKLL IEMEKRLEKG QSIDDLMPAQ K 381
    Length:381
    Mass (Da):42,871
    Last modified:August 13, 1987 - v1
    Checksum:i313BCCB46BCDD02B
    GO
    Isoform Ba-CK (identifier: P05122-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-65: MPFSNSHNLL...VIQTGVDNPG → MAQLNNQRLP...VIQTGVDNPG

    Show »
    Length:376
    Mass (Da):42,267
    Checksum:i5A107A22ABCB046F
    GO
    Isoform Bb-CK-2 (identifier: P05122-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-11: Missing.

    Show »
    Length:370
    Mass (Da):41,601
    Checksum:iA08833EFEBB99797
    GO
    Isoform Bb-CK-3 (identifier: P05122-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-29: Missing.

    Show »
    Length:352
    Mass (Da):39,456
    Checksum:iD0BE655ACDE7C082
    GO
    Isoform Bb-CK-4 (identifier: P05122-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-69: Missing.

    Show »
    Length:312
    Mass (Da):34,983
    Checksum:i0FDE036FF4AD60C6
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6969Missing in isoform Bb-CK-4. CuratedVSP_010770Add
    BLAST
    Alternative sequencei1 – 6565MPFSN…VDNPG → MAQLNNQRLPPEEEYPDLST HNNHMAKVLTLDLYKKLRDR VTPSGFTLDDVIQTGVDNPG in isoform Ba-CK. CuratedVSP_002814Add
    BLAST
    Alternative sequencei1 – 2929Missing in isoform Bb-CK-3. CuratedVSP_010769Add
    BLAST
    Alternative sequencei1 – 1111Missing in isoform Bb-CK-2. CuratedVSP_010768Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03509 mRNA. Translation: CAA27221.1.
    M33714
    , M33711, M33712, M33713 Genomic DNA. Translation: AAA48613.1.
    M33714
    , M33711, M33712, M33713 Genomic DNA. Translation: AAA48614.1.
    M35381 Genomic DNA. Translation: AAA48687.1.
    PIRiA37059. A24793.
    RefSeqiNP_990641.1. NM_205310.1. [P05122-1]
    UniGeneiGga.2722.

    Genome annotation databases

    GeneIDi396248.
    KEGGigga:396248.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03509 mRNA. Translation: CAA27221.1 .
    M33714
    , M33711 , M33712 , M33713 Genomic DNA. Translation: AAA48613.1 .
    M33714
    , M33711 , M33712 , M33713 Genomic DNA. Translation: AAA48614.1 .
    M35381 Genomic DNA. Translation: AAA48687.1 .
    PIRi A37059. A24793.
    RefSeqi NP_990641.1. NM_205310.1. [P05122-1 ]
    UniGenei Gga.2722.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1QH4 X-ray 1.41 A/B/C/D 2-381 [» ]
    ProteinModelPortali P05122.
    SMRi P05122. Positions 2-381.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 676508. 1 interaction.
    IntActi P05122. 1 interaction.

    Proteomic databases

    PaxDbi P05122.
    PRIDEi P05122.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 396248.
    KEGGi gga:396248.

    Organism-specific databases

    CTDi 1152.

    Phylogenomic databases

    eggNOGi COG3869.
    HOGENOMi HOG000232165.
    HOVERGENi HBG001339.
    KOi K00933.
    PhylomeDBi P05122.

    Miscellaneous databases

    EvolutionaryTracei P05122.
    NextBioi 20816300.
    PROi P05122.

    Family and domain databases

    Gene3Di 1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 1 hit.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The primary structure of chicken B-creatine kinase and evidence for heterogeneity of its mRNA."
      Hossle J.P., Rosenberg U.B., Schaefer B.W., Eppenberger H.M., Perriard J.-C.
      Nucleic Acids Res. 14:1449-1463(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BB-CK-1).
    2. "A unique chicken B-creatine kinase gene gives rise to two B-creatine kinase isoproteins with distinct N termini by alternative splicing."
      Wirz T., Braendle U., Soldati T., Hossle J.P., Perriard J.-C.
      J. Biol. Chem. 265:11656-11666(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS BA-CK AND BB-CK-1).
      Strain: White leghorn.
      Tissue: Brain.
    3. "Accumulation of creatine kinase mRNA during myogenesis: molecular cloning of a B-creatine kinase cDNA."
      Kwiatkowski R.W., Ehrismann R., Schweinfest C.W., Dottin R.P.
      Dev. Biol. 112:84-88(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 317-381.
      Tissue: Brain.
    4. "Autophosphorylation of creatine kinase: characterization and identification of a specifically phosphorylated peptide."
      Hemmer W., Furter-Graves E.M., Frank G., Wallimann T., Furter R.
      Biochim. Biophys. Acta 1251:81-90(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-9; 12-20; 266-277 AND 320-329, AUTOPHOSPHORYLATION.
    5. "Alternative ribosomal initiation gives rise to chicken brain-type creatine kinase isoproteins with heterogeneous amino termini."
      Soldati T., Schaefer B.W., Perriard J.-C.
      J. Biol. Chem. 265:4498-4506(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING (ISOFORMS BA-CK-2; BA-CK-3 AND BA-CK-4), PHOSPHORYLATION.
    6. "Crystal structure of brain-type creatine kinase at 1.41-A resolution."
      Eder M., Schlattner U., Becker A., Wallimann T., Kabsch W., Fritz-Wolf K.
      Protein Sci. 8:2258-2269(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.41 ANGSTROMS).

    Entry informationi

    Entry nameiKCRB_CHICK
    AccessioniPrimary (citable) accession number: P05122
    Secondary accession number(s): Q92061
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3