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P05120 (PAI2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 158. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plasminogen activator inhibitor 2

Short name=PAI-2
Alternative name(s):
Monocyte Arg-serpin
Placental plasminogen activator inhibitor
Serpin B2
Urokinase inhibitor
Gene names
Name:SERPINB2
Synonyms:PAI2, PLANH2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

Subunit structure

Interacts with PSMB1. Ref.9

Subcellular location

Cytoplasm. Secretedextracellular space.

Post-translational modification

The signal sequence is not cleaved.

Sequence similarities

Belongs to the serpin family. Ov-serpin subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Plasminogen activator inhibitor 2
PRO_0000223296
Signal peptide1 – ?Not cleaved

Sites

Site380 – 3812Reactive bond

Amino acid modifications

Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation1151N-linked (GlcNAc...) Potential
Glycosylation3391N-linked (GlcNAc...) Potential
Disulfide bond5 ↔ 405

Natural variations

Natural variant1201N → D. Ref.12
Corresponds to variant rs6098 [ dbSNP | Ensembl ].
VAR_011743
Natural variant2291R → H. Ref.12
Corresponds to variant rs6100 [ dbSNP | Ensembl ].
VAR_014173
Natural variant3741G → A.
Corresponds to variant rs34066931 [ dbSNP | Ensembl ].
VAR_051946
Natural variant4041N → K. Ref.12
Corresponds to variant rs6103 [ dbSNP | Ensembl ].
VAR_011744
Natural variant4131S → C. Ref.12
Corresponds to variant rs6104 [ dbSNP | Ensembl ].
VAR_011745

Experimental info

Sequence conflict1701N → Y in AAH12609. Ref.7

Secondary structure

................................................................... 415
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P05120 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 10DFAB5A4B1246FF

FASTA41546,596
        10         20         30         40         50         60 
MEDLCVANTL FALNLFKHLA KASPTQNLFL SPWSISSTMA MVYMGSRGST EDQMAKVLQF 

        70         80         90        100        110        120 
NEVGANAVTP MTPENFTSCG FMQQIQKGSY PDAILQAQAA DKIHSSFRSL SSAINASTGN 

       130        140        150        160        170        180 
YLLESVNKLF GEKSASFREE YIRLCQKYYS SEPQAVDFLE CAEEARKKIN SWVKTQTKGK 

       190        200        210        220        230        240 
IPNLLPEGSV DGDTRMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSAQRT PVQMMYLREK 

       250        260        270        280        290        300 
LNIGYIEDLK AQILELPYAG DVSMFLLLPD EIADVSTGLE LLESEITYDK LNKWTSKDKM 

       310        320        330        340        350        360 
AEDEVEVYIP QFKLEEHYEL RSILRSMGME DAFNKGRANF SGMSERNDLF LSEVFHQAMV 

       370        380        390        400        410 
DVNEEGTEAA AGTGGVMTGR TGHGGPQFVA DHPFLFLIMH KITNCILFFG RFSSP 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning and expression in Escherichia coli of a plasminogen activator inhibitor from human placenta."
Ye R.D., Wun T.-Z., Sadler J.E.
J. Biol. Chem. 262:3718-3725(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[2]"Plasminogen activator inhibitor 2: regulation of gene transcription during phorbol ester-mediated differentiation of U-937 human histiocytic lymphoma cells."
Schleuning W.-D., Medcalf R.L., Hession C., Rothenbuhler R., Shaw A., Kruithof E.K.O.
Mol. Cell. Biol. 7:4564-4567(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Human monocyte Arg-Serpin cDNA. Sequence, chromosomal assignment, and homology to plasminogen activator-inhibitor."
Webb A.C., Collins K.L., Snyder S.F., Alexander S.J., Rosenwasser L.J., Eddy R.L., Shows T.B., Auron P.E.
J. Exp. Med. 166:77-94(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Monocyte.
[4]"Cloning and expression of a cDNA coding for a human monocyte-derived plasminogen activator inhibitor."
Antalis T.M., Clark M.A., Barnes T., Lehrbach P.R., Devine P.L., Schevzov G., Goss N.H., Stephens R.W., Tolstoshev P.
Proc. Natl. Acad. Sci. U.S.A. 85:985-989(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Monocyte.
[5]"Structure of the gene for human plasminogen activator inhibitor-2. The nearest mammalian homologue of chicken ovalbumin."
Ye R.D., Ahern S.M., le Beau M.M., Lebo R.V., Sadler J.E.
J. Biol. Chem. 264:5495-5502(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Chromosomal organization and localization of the human urokinase inhibitor gene: perfect structural conservation with ovalbumin."
Samia J.A., Alexander S.J., Horton K.W., Auron P.E., Byers M.G., Shows T.B. Jr., Webb A.C.
Genomics 6:159-167(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[8]"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."
Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.
Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 12-17; 103-108 AND 314-321.
[9]"Interaction of plasminogen activator inhibitor-2 and proteasome subunit, beta type 1."
Fan J., Zhang Y.Q., Li P., Hou M., Tan L., Wang X., Zhu Y.S.
Acta Biochim. Biophys. Sin. 36:42-46(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PSMB1.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"The crystal structure of plasminogen activator inhibitor 2 at 2.0-A resolution: implications for serpin function."
Harrop S.J., Jankova L., Coles M., Jardine D., Whittaker J.S., Gould A.R., Meister A., King G.C., Mabbutt B.C., Curmi P.M.G.
Structure 7:43-54(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[12]"Characterization of single-nucleotide polymorphisms in coding regions of human genes."
Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S.
Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS ASP-120; HIS-229; LYS-404 AND CYS-413.
[13]Erratum
Cargill M., Altshuler D., Ireland J., Sklar P., Ardlie K., Patil N., Shaw N., Lane C.R., Lim E.P., Kalyanaraman N., Nemesh J., Ziaugra L., Friedland L., Rolfe A., Warrington J., Lipshutz R., Daley G.Q., Lander E.S.
Nat. Genet. 23:373-373(1999)
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02685 mRNA. Translation: AAA36413.1.
M18082 mRNA. Translation: AAA60006.1.
Y00630 mRNA. Translation: CAA68666.1.
J03603 mRNA. Translation: AAA60004.1.
M24657 expand/collapse EMBL AC list , M24651, M24652, M24653, M24654, M24655, M24656 Genomic DNA. Translation: AAA60348.1.
M31551 expand/collapse EMBL AC list , M31547, M31548, M31549, M31550 Genomic DNA. Translation: AAA36797.1.
BC012609 mRNA. Translation: AAH12609.1.
PIRA32853.
RefSeqNP_001137290.1. NM_001143818.1.
NP_002566.1. NM_002575.2.
UniGeneHs.594481.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BY7X-ray2.00A1-415[»]
1JRRX-ray1.60A1-415[»]
P367-380[»]
2ARQX-ray1.85A1-415[»]
P367-380[»]
2ARRX-ray1.55A1-415[»]
P367-380[»]
ProteinModelPortalP05120.
SMRP05120. Positions 3-415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111092. 6 interactions.
IntActP05120. 1 interaction.
MINTMINT-270968.
STRING9606.ENSP00000299502.

Chemistry

DrugBankDB00009. Alteplase.
DB00029. Anistreplase.
DB00015. Reteplase.
DB00031. Tenecteplase.
DB00013. Urokinase.

Protein family/group databases

MEROPSI04.007.

PTM databases

PhosphoSiteP05120.

Polymorphism databases

DMDM1352712.

Proteomic databases

PaxDbP05120.
PRIDEP05120.

Protocols and materials databases

DNASU5055.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000299502; ENSP00000299502; ENSG00000197632.
ENST00000457692; ENSP00000401645; ENSG00000197632.
GeneID5055.
KEGGhsa:5055.
UCSCuc002ljo.3. human.

Organism-specific databases

CTD5055.
GeneCardsGC18P061528.
H-InvDBHIX0212740.
HGNCHGNC:8584. SERPINB2.
HPAHPA015480.
MIM173390. gene.
neXtProtNX_P05120.
PharmGKBPA35500.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG4826.
HOGENOMHOG000238519.
HOVERGENHBG005957.
InParanoidP05120.
KOK13963.
OMALFLIMHK.
PhylomeDBP05120.
TreeFamTF352619.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.

Gene expression databases

ArrayExpressP05120.
BgeeP05120.
CleanExHS_SERPINB2.
GenevestigatorP05120.

Family and domain databases

InterProIPR015556. PAI-2.
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PTHR11461:SF61. PTHR11461:SF61. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 2 hits.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSERPINB2. human.
EvolutionaryTraceP05120.
GeneWikiPlasminogen_activator_inhibitor-2.
GenomeRNAi5055.
NextBio19484.
PROP05120.
SOURCESearch...

Entry information

Entry namePAI2_HUMAN
AccessionPrimary (citable) accession number: P05120
Secondary accession number(s): Q96E96
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 18

Human chromosome 18: entries, gene names and cross-references to MIM