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P05120

- PAI2_HUMAN

UniProt

P05120 - PAI2_HUMAN

Protein

Plasminogen activator inhibitor 2

Gene

SERPINB2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 162 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei380 – 3812Reactive bond

    GO - Molecular functioni

    1. serine-type endopeptidase inhibitor activity Source: RefGenome

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. fibrinolysis Source: Reactome
    3. negative regulation of apoptotic process Source: ProtInc
    4. negative regulation of endopeptidase activity Source: RefGenome
    5. regulation of proteolysis Source: RefGenome

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Keywords - Biological processi

    Plasminogen activation

    Enzyme and pathway databases

    ReactomeiREACT_641. Dissolution of Fibrin Clot.

    Protein family/group databases

    MEROPSiI04.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plasminogen activator inhibitor 2
    Short name:
    PAI-2
    Alternative name(s):
    Monocyte Arg-serpin
    Placental plasminogen activator inhibitor
    Serpin B2
    Urokinase inhibitor
    Gene namesi
    Name:SERPINB2
    Synonyms:PAI2, PLANH2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:8584. SERPINB2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. extracellular region Source: Reactome
    3. extracellular space Source: UniProtKB-SubCell
    4. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35500.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 415415Plasminogen activator inhibitor 2PRO_0000223296Add
    BLAST
    Signal peptidei1 – ?Not cleaved

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi5 ↔ 405
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi115 – 1151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi339 – 3391N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The signal sequence is not cleaved.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP05120.
    PaxDbiP05120.
    PRIDEiP05120.

    PTM databases

    PhosphoSiteiP05120.

    Expressioni

    Gene expression databases

    ArrayExpressiP05120.
    BgeeiP05120.
    CleanExiHS_SERPINB2.
    GenevestigatoriP05120.

    Organism-specific databases

    HPAiHPA015480.

    Interactioni

    Subunit structurei

    Interacts with PSMB1.1 Publication

    Protein-protein interaction databases

    BioGridi111092. 6 interactions.
    IntActiP05120. 1 interaction.
    MINTiMINT-270968.
    STRINGi9606.ENSP00000299502.

    Structurei

    Secondary structure

    1
    415
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 2217
    Beta strandi24 – 263
    Beta strandi28 – 303
    Helixi32 – 4514
    Helixi48 – 5710
    Turni58 – 625
    Helixi100 – 1023
    Helixi103 – 11513
    Beta strandi122 – 13211
    Helixi139 – 14911
    Beta strandi154 – 1563
    Helixi158 – 17619
    Turni177 – 1793
    Beta strandi194 – 20512
    Beta strandi208 – 2103
    Beta strandi220 – 2256
    Beta strandi228 – 2325
    Beta strandi234 – 24613
    Helixi247 – 2493
    Beta strandi251 – 26919
    Beta strandi275 – 2784
    Helixi280 – 2856
    Helixi288 – 2958
    Turni297 – 2993
    Beta strandi301 – 31010
    Beta strandi312 – 3198
    Helixi321 – 3277
    Helixi331 – 3333
    Turni335 – 3373
    Turni341 – 3433
    Beta strandi349 – 36214
    Beta strandi368 – 37912
    Beta strandi387 – 3893
    Beta strandi394 – 4007
    Turni401 – 4044
    Beta strandi405 – 4128

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BY7X-ray2.00A1-415[»]
    1JRRX-ray1.60A1-415[»]
    P367-380[»]
    2ARQX-ray1.85A1-415[»]
    P367-380[»]
    2ARRX-ray1.55A1-415[»]
    P367-380[»]
    ProteinModelPortaliP05120.
    SMRiP05120. Positions 3-415.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05120.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the serpin family. Ov-serpin subfamily.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4826.
    HOGENOMiHOG000238519.
    HOVERGENiHBG005957.
    InParanoidiP05120.
    KOiK13963.
    OMAiLFLIMHK.
    PhylomeDBiP05120.
    TreeFamiTF352619.

    Family and domain databases

    InterProiIPR015556. PAI-2.
    IPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view]
    PANTHERiPTHR11461. PTHR11461. 1 hit.
    PTHR11461:SF61. PTHR11461:SF61. 1 hit.
    PfamiPF00079. Serpin. 1 hit.
    [Graphical view]
    SMARTiSM00093. SERPIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF56574. SSF56574. 2 hits.
    PROSITEiPS00284. SERPIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05120-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDLCVANTL FALNLFKHLA KASPTQNLFL SPWSISSTMA MVYMGSRGST    50
    EDQMAKVLQF NEVGANAVTP MTPENFTSCG FMQQIQKGSY PDAILQAQAA 100
    DKIHSSFRSL SSAINASTGN YLLESVNKLF GEKSASFREE YIRLCQKYYS 150
    SEPQAVDFLE CAEEARKKIN SWVKTQTKGK IPNLLPEGSV DGDTRMVLVN 200
    AVYFKGKWKT PFEKKLNGLY PFRVNSAQRT PVQMMYLREK LNIGYIEDLK 250
    AQILELPYAG DVSMFLLLPD EIADVSTGLE LLESEITYDK LNKWTSKDKM 300
    AEDEVEVYIP QFKLEEHYEL RSILRSMGME DAFNKGRANF SGMSERNDLF 350
    LSEVFHQAMV DVNEEGTEAA AGTGGVMTGR TGHGGPQFVA DHPFLFLIMH 400
    KITNCILFFG RFSSP 415
    Length:415
    Mass (Da):46,596
    Last modified:February 1, 1996 - v2
    Checksum:i10DFAB5A4B1246FF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti170 – 1701N → Y in AAH12609. (PubMed:15489334)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti120 – 1201N → D.1 Publication
    Corresponds to variant rs6098 [ dbSNP | Ensembl ].
    VAR_011743
    Natural varianti229 – 2291R → H.1 Publication
    Corresponds to variant rs6100 [ dbSNP | Ensembl ].
    VAR_014173
    Natural varianti374 – 3741G → A.
    Corresponds to variant rs34066931 [ dbSNP | Ensembl ].
    VAR_051946
    Natural varianti404 – 4041N → K.1 Publication
    Corresponds to variant rs6103 [ dbSNP | Ensembl ].
    VAR_011744
    Natural varianti413 – 4131S → C.1 Publication
    Corresponds to variant rs6104 [ dbSNP | Ensembl ].
    VAR_011745

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02685 mRNA. Translation: AAA36413.1.
    M18082 mRNA. Translation: AAA60006.1.
    Y00630 mRNA. Translation: CAA68666.1.
    J03603 mRNA. Translation: AAA60004.1.
    M24657
    , M24651, M24652, M24653, M24654, M24655, M24656 Genomic DNA. Translation: AAA60348.1.
    M31551
    , M31547, M31548, M31549, M31550 Genomic DNA. Translation: AAA36797.1.
    BC012609 mRNA. Translation: AAH12609.1.
    CCDSiCCDS11989.1.
    PIRiA32853.
    RefSeqiNP_001137290.1. NM_001143818.1.
    NP_002566.1. NM_002575.2.
    UniGeneiHs.594481.

    Genome annotation databases

    EnsembliENST00000299502; ENSP00000299502; ENSG00000197632.
    ENST00000457692; ENSP00000401645; ENSG00000197632.
    GeneIDi5055.
    KEGGihsa:5055.
    UCSCiuc002ljo.3. human.

    Polymorphism databases

    DMDMi1352712.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02685 mRNA. Translation: AAA36413.1 .
    M18082 mRNA. Translation: AAA60006.1 .
    Y00630 mRNA. Translation: CAA68666.1 .
    J03603 mRNA. Translation: AAA60004.1 .
    M24657
    , M24651 , M24652 , M24653 , M24654 , M24655 , M24656 Genomic DNA. Translation: AAA60348.1 .
    M31551
    , M31547 , M31548 , M31549 , M31550 Genomic DNA. Translation: AAA36797.1 .
    BC012609 mRNA. Translation: AAH12609.1 .
    CCDSi CCDS11989.1.
    PIRi A32853.
    RefSeqi NP_001137290.1. NM_001143818.1.
    NP_002566.1. NM_002575.2.
    UniGenei Hs.594481.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BY7 X-ray 2.00 A 1-415 [» ]
    1JRR X-ray 1.60 A 1-415 [» ]
    P 367-380 [» ]
    2ARQ X-ray 1.85 A 1-415 [» ]
    P 367-380 [» ]
    2ARR X-ray 1.55 A 1-415 [» ]
    P 367-380 [» ]
    ProteinModelPortali P05120.
    SMRi P05120. Positions 3-415.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111092. 6 interactions.
    IntActi P05120. 1 interaction.
    MINTi MINT-270968.
    STRINGi 9606.ENSP00000299502.

    Chemistry

    DrugBanki DB00009. Alteplase.
    DB00029. Anistreplase.
    DB00015. Reteplase.
    DB00031. Tenecteplase.
    DB00013. Urokinase.

    Protein family/group databases

    MEROPSi I04.007.

    PTM databases

    PhosphoSitei P05120.

    Polymorphism databases

    DMDMi 1352712.

    Proteomic databases

    MaxQBi P05120.
    PaxDbi P05120.
    PRIDEi P05120.

    Protocols and materials databases

    DNASUi 5055.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000299502 ; ENSP00000299502 ; ENSG00000197632 .
    ENST00000457692 ; ENSP00000401645 ; ENSG00000197632 .
    GeneIDi 5055.
    KEGGi hsa:5055.
    UCSCi uc002ljo.3. human.

    Organism-specific databases

    CTDi 5055.
    GeneCardsi GC18P061528.
    H-InvDB HIX0212740.
    HGNCi HGNC:8584. SERPINB2.
    HPAi HPA015480.
    MIMi 173390. gene.
    neXtProti NX_P05120.
    PharmGKBi PA35500.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG4826.
    HOGENOMi HOG000238519.
    HOVERGENi HBG005957.
    InParanoidi P05120.
    KOi K13963.
    OMAi LFLIMHK.
    PhylomeDBi P05120.
    TreeFami TF352619.

    Enzyme and pathway databases

    Reactomei REACT_641. Dissolution of Fibrin Clot.

    Miscellaneous databases

    ChiTaRSi SERPINB2. human.
    EvolutionaryTracei P05120.
    GeneWikii Plasminogen_activator_inhibitor-2.
    GenomeRNAii 5055.
    NextBioi 19484.
    PROi P05120.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P05120.
    Bgeei P05120.
    CleanExi HS_SERPINB2.
    Genevestigatori P05120.

    Family and domain databases

    InterProi IPR015556. PAI-2.
    IPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view ]
    PANTHERi PTHR11461. PTHR11461. 1 hit.
    PTHR11461:SF61. PTHR11461:SF61. 1 hit.
    Pfami PF00079. Serpin. 1 hit.
    [Graphical view ]
    SMARTi SM00093. SERPIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56574. SSF56574. 2 hits.
    PROSITEi PS00284. SERPIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression in Escherichia coli of a plasminogen activator inhibitor from human placenta."
      Ye R.D., Wun T.-Z., Sadler J.E.
      J. Biol. Chem. 262:3718-3725(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Placenta.
    2. "Plasminogen activator inhibitor 2: regulation of gene transcription during phorbol ester-mediated differentiation of U-937 human histiocytic lymphoma cells."
      Schleuning W.-D., Medcalf R.L., Hession C., Rothenbuhler R., Shaw A., Kruithof E.K.O.
      Mol. Cell. Biol. 7:4564-4567(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Human monocyte Arg-Serpin cDNA. Sequence, chromosomal assignment, and homology to plasminogen activator-inhibitor."
      Webb A.C., Collins K.L., Snyder S.F., Alexander S.J., Rosenwasser L.J., Eddy R.L., Shows T.B., Auron P.E.
      J. Exp. Med. 166:77-94(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Monocyte.
    4. "Cloning and expression of a cDNA coding for a human monocyte-derived plasminogen activator inhibitor."
      Antalis T.M., Clark M.A., Barnes T., Lehrbach P.R., Devine P.L., Schevzov G., Goss N.H., Stephens R.W., Tolstoshev P.
      Proc. Natl. Acad. Sci. U.S.A. 85:985-989(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Monocyte.
    5. "Structure of the gene for human plasminogen activator inhibitor-2. The nearest mammalian homologue of chicken ovalbumin."
      Ye R.D., Ahern S.M., le Beau M.M., Lebo R.V., Sadler J.E.
      J. Biol. Chem. 264:5495-5502(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "Chromosomal organization and localization of the human urokinase inhibitor gene: perfect structural conservation with ovalbumin."
      Samia J.A., Alexander S.J., Horton K.W., Auron P.E., Byers M.G., Shows T.B. Jr., Webb A.C.
      Genomics 6:159-167(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    8. "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."
      Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.
      Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 12-17; 103-108 AND 314-321.
    9. "Interaction of plasminogen activator inhibitor-2 and proteasome subunit, beta type 1."
      Fan J., Zhang Y.Q., Li P., Hou M., Tan L., Wang X., Zhu Y.S.
      Acta Biochim. Biophys. Sin. 36:42-46(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PSMB1.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "The crystal structure of plasminogen activator inhibitor 2 at 2.0-A resolution: implications for serpin function."
      Harrop S.J., Jankova L., Coles M., Jardine D., Whittaker J.S., Gould A.R., Meister A., King G.C., Mabbutt B.C., Curmi P.M.G.
      Structure 7:43-54(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    12. Cited for: VARIANTS ASP-120; HIS-229; LYS-404 AND CYS-413.

    Entry informationi

    Entry nameiPAI2_HUMAN
    AccessioniPrimary (citable) accession number: P05120
    Secondary accession number(s): Q96E96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 162 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3