Reviewed, UniProtKB/Swiss-Prot P05109 (S10A8_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein S100-A8
Alternative name(s):
    S100 calcium-binding protein A8
    Calgranulin-A
    Migration inhibitory factor-related protein 8
      Short name=MRP-8
      Short name=P8
    Cystic fibrosis antigen
      Short name=CFAG
    Leukocyte L1 complex light chain
    Calprotectin L1L subunit
    Urinary stone protein band A

Gene names

Name:	S100A8
Synonyms:	CAGA, CFAG, MRP8

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	93 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells.
Subunit structure	Homodimer.
Miscellaneous	Binds two calcium ions per molecule with an affinity similar to that of the S100 proteins.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	inflammatory response Ref.2 Traceable author statement. Source: ProtInc
Molecular function	calcium ion binding Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LRRK1	Q38SD2	1	EBI-355281,EBI-1050422
MOBKL1B	Q9H8S9	1	EBI-355281,EBI-748229
NUAK1	O60285	1	EBI-355281,EBI-1046789
PPM1B	O75688	1	EBI-355281,EBI-1047039
USF2	Q15853	1	EBI-355281,EBI-1055994

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 93

93

Protein S100-A8

PRO_0000143993

Regions

Domain

12 – 47

36

EF-hand 1

Domain

46 – 81

36

EF-hand 2

Calcium binding

20 – 33

14

1; low affinity

Calcium binding

59 – 70

12

2; high affinity

Experimental info

Sequence conflict

80 – 93

14

VAAHK…ESHKE → WQPTKKAMKKATKSS in CAA68390. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

93

Helix Strand Turn

Details...

Helix

4 – 20

17

Beta strand

22 – 25

4

Helix

31 – 41

11

Helix

44 – 47

4

Helix

51 – 58

8

Beta strand

63 – 66

4

Helix

68 – 86

19

Sequences

	Sequence		Length	Mass (Da)
	P05109-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: 78F589140B9CE166	FASTA	93	10,835
	10 20 30 40 50 60 MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDI 70 80 90 NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A clue to the basic defect in cystic fibrosis from cloning the CF antigen gene." Dorin J.R., Novak M., Hill R.E., Brock D.J.H., Secher D.S., van Heyningen V. Nature 326:614-617(1987) [PubMed: 3561500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis." Odink K., Cerletti N., Bruggen J., Clerc R.G., Tarcsay L., Zwaldo G., Gerhards G., Schlegel R., Sorg C. Nature 330:80-82(1987) [PubMed: 3313057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation." Lagasse E., Clerc R.G. Mol. Cell. Biol. 8:2402-2410(1988) [PubMed: 3405210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The calcium-binding protein MRP-8 is produced by human pulmonary tumor cells." Schaefer T., Sachse G.E., Gassen H.G. Biol. Chem. Hoppe-Seyler 372:1-4(1991) [PubMed: 2039599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-30.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tongue.
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[9]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skeletal muscle.
[11]	"Host-parasite interaction in human onchocerciasis: identification and sequence analysis of a novel human calgranulin." Marti T., Erttmann K.D., Gallin M.Y. Biochem. Biophys. Res. Commun. 221:454-458(1996) [PubMed: 8619876] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-19; 24-35 AND 63-89.
[12]	"In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena." Miyasaki K.T., Bodeau A.L., Murthy A.R., Lehrer R.I. J. Dent. Res. 72:517-523(1993) [PubMed: 8423249] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-27.
[13]	"Translocation of a small cytosolic calcium-binding protein (MRP-8) to plasma membrane correlates with human neutrophil activation." Lemarchand P., Vaglio M., Mauel J., Markert M. J. Biol. Chem. 267:19379-19382(1992) [PubMed: 1326551] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25. Tissue: Neutrophil.
[14]	"Calprotectin-like protein is related to soluble organic matrix in calcium oxalate urinary stone." Umekawa T., Kurita T. Biochem. Mol. Biol. Int. 34:309-313(1994) [PubMed: 7849642] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20.
[15]	"Isolation of an ascitic oncodevelopmental protein exhibiting high sequence homology with calcium-binding protein MRP8." Nakai M., Ishikawa M., Hamada Y., Sugano S. Biol. Chem. Hoppe-Seyler 375:789-792(1994) [PubMed: 7695842] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20. Tissue: Ascites.
[16]	"Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes." Rasmussen H.H., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J. Electrophoresis 13:960-969(1992) [PubMed: 1286667] [Abstract] Cited for: PROTEIN SEQUENCE OF 38-47 AND 50-56. Tissue: Keratinocyte.
[17]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[18]	"The structure of human MRP8, a member of the S100 calcium-binding protein family, by MAD phasing at 1.9 A resolution." Ishikawa K., Nakagawa A., Tanaka I., Suzuki M., Nishihira J. Acta Crystallogr. D 56:559-566(2000) [PubMed: 10771424] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y00278 mRNA. Translation: CAA68390.1.
X06234 mRNA. Translation: CAA29580.1.
M21005 Genomic DNA. Translation: AAA36327.1.
AK291328 mRNA. Translation: BAF84017.1.
CR407674 mRNA. Translation: CAG28602.1.
BT007378 mRNA. Translation: AAP36042.1.
AL591704 Genomic DNA. Translation: CAI19497.1.
CH471121 Genomic DNA. Translation: EAW53330.1.
BC005928 mRNA. Translation: AAH05928.1.

IPI

IPI00007047.

PIR

BCHUCF. A31848.

RefSeq

NP_002955.2.

UniGene

Hs.416073

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MR8	X-ray	1.90	A/B	1-93	[»]
1XK4	X-ray	1.80	A/B/E/F/I/J	1-93	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1165N.

IntAct

P05109. 25 interactions.

2-D gel databases

SWISS-2DPAGE

P05109.

Aarhus/Ghent-2DPAGE

1003. IEF.

PMMA-2DPAGE

P05109.

Proteomic databases

PeptideAtlas

P05109.

PRIDE

P05109.

Genome annotation databases

Ensembl

ENSG00000143546. Homo sapiens. [Contig view]

GeneID

6279.

KEGG

hsa:6279.

Organism-specific databases

GeneCards

GC01M151629.

H-InvDB

HIX0001079.

HGNC

HGNC:10498. S100A8.

HPA

CAB002791.

MIM

123885. gene.

PharmGKB

PA34910.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P05109.

HOVERGEN

P05109.

OMA

P05109. KDADTWF.

Gene expression databases

ArrayExpress

P05109.

Bgee

P05109.

CleanEx

HS_S100A8.

GermOnline

ENSG00000143546. Homo sapiens.

Family and domain databases

InterPro

IPR011992. EF-Hand_type.
IPR018248. EF_hand.
IPR018247. EF_HAND_1.
IPR018249. EF_HAND_2.
IPR001751. S100_Ca_bd.
IPR013787. S100_Ca_bd_sub.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

Pfam

PF00036. efhand. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]

ProDom

PD003407. CaBP_S100. 1 hit.
PD000012. EF-hand. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

24373.

SOURCE

Search...

Entry information

Entry name

S10A8_HUMAN

Accession

Primary (citable) accession number: P05109
Secondary accession number(s): A8K5L3 , Q5SY70, Q9UC84, Q9UC92, Q9UCJ0, Q9UCM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 1, 1988
Last modified:	June 16, 2009
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)