Reviewed, UniProtKB/Swiss-Prot P05104 (T2P7_PSEAE)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme PaeR7I
      Short name=R.PaeR7I
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme PaeR7I
    Endonuclease PaeR7I

Gene names

Name:

paeR7IR

Encoded on

Plasmid pMG7

Organism

Pseudomonas aeruginosa

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the type II restriction enzyme XhoI family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 246	245	Type-2 restriction enzyme PaeR7I		PRO_0000077353

Sequences

	Sequence		Length	Mass (Da)
	P05104-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 3BC63AC987BDF3BA	FASTA	246	27,279
	10 20 30 40 50 60 MALDLVDYEQ KARDAVKAFW GNREAARQKQ IESGKADQGE RAGVTGGKNM DGFLALVLDV 70 80 90 100 110 120 IKANGLAHAE IHQNRAMLTL PGYFRPTKLW DLLVIYKGEL IAAIELKSHV GPSFSNNFNN 130 140 150 160 170 180 RTEEAIGTAH DLWTAYREEA FGKQPRPFVG WLMMVEDAPE SRRPVRDSSP HFPVFEEFKG 190 200 210 220 230 240 ASYLTRYDLL CQRLVQEQLY TTAAVIAAER SAVDTGNFTE LSSMTSLKTF VSALAGHIAA EAARLG

References

[1]

"Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products." Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., Gingeras T.R. Nucleic Acids Res. 13:8441-8461(1985) [PubMed: 3001639] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03274 Genomic DNA. Translation: CAA27026.1.
PIR	NDPS7A. S07935.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1451. PaeR7I.
Enzyme and pathway databases
BRENDA	3.1.21.4. 354.
Family and domain databases
InterPro	IPR007636. Restrict_endonuc_II_XhoI. [Graphical view]
Pfam	PF04555. XhoI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2P7_PSEAE

Accession

Primary (citable) accession number: P05104

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 61 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)