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P05104

- T2P7_PSEAI

UniProt

P05104 - T2P7_PSEAI

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Protein

Type-2 restriction enzyme PaeR7I

Gene
paeR7IR
Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Magnesium.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Protein family/group databases

REBASEi1451. PaeR7I.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme PaeR7I (EC:3.1.21.4)
Short name:
R.PaeR7I
Alternative name(s):
Endonuclease PaeR7I
Type II restriction enzyme PaeR7I
Gene namesi
Name:paeR7IR
Encoded oniPlasmid pMG70 Publication
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 246245Type-2 restriction enzyme PaeR7IPRO_0000077353Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR007636. Restrct_endonuc_II_XhoI.
[Graphical view]
PfamiPF04555. XhoI. 1 hit.
[Graphical view]
PIRSFiPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05104-1 [UniParc]FASTAAdd to Basket

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MALDLVDYEQ KARDAVKAFW GNREAARQKQ IESGKADQGE RAGVTGGKNM    50
DGFLALVLDV IKANGLAHAE IHQNRAMLTL PGYFRPTKLW DLLVIYKGEL 100
IAAIELKSHV GPSFSNNFNN RTEEAIGTAH DLWTAYREEA FGKQPRPFVG 150
WLMMVEDAPE SRRPVRDSSP HFPVFEEFKG ASYLTRYDLL CQRLVQEQLY 200
TTAAVIAAER SAVDTGNFTE LSSMTSLKTF VSALAGHIAA EAARLG 246
Length:246
Mass (Da):27,279
Last modified:January 23, 2007 - v3
Checksum:i3BC63AC987BDF3BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03274 Genomic DNA. Translation: CAA27026.1.
PIRiS07935. NDPS7A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03274 Genomic DNA. Translation: CAA27026.1 .
PIRi S07935. NDPS7A.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1451. PaeR7I.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007636. Restrct_endonuc_II_XhoI.
[Graphical view ]
Pfami PF04555. XhoI. 1 hit.
[Graphical view ]
PIRSFi PIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products."
    Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., Gingeras T.R.
    Nucleic Acids Res. 13:8441-8461(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiT2P7_PSEAI
AccessioniPrimary (citable) accession number: P05104
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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