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P05104 (T2P7_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme PaeR7I

Short name=R.PaeR7I
EC=3.1.21.4
Alternative name(s):
Endonuclease PaeR7I
Type II restriction enzyme PaeR7I
Gene names
Name:paeR7IR
Encoded onPlasmid pMG7
OrganismPseudomonas aeruginosa
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the type II restriction enzyme XhoI family.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Plasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 246245Type-2 restriction enzyme PaeR7I
PRO_0000077353

Sequences

Sequence LengthMass (Da)Tools
P05104 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 3BC63AC987BDF3BA

FASTA24627,279
        10         20         30         40         50         60 
MALDLVDYEQ KARDAVKAFW GNREAARQKQ IESGKADQGE RAGVTGGKNM DGFLALVLDV 

        70         80         90        100        110        120 
IKANGLAHAE IHQNRAMLTL PGYFRPTKLW DLLVIYKGEL IAAIELKSHV GPSFSNNFNN 

       130        140        150        160        170        180 
RTEEAIGTAH DLWTAYREEA FGKQPRPFVG WLMMVEDAPE SRRPVRDSSP HFPVFEEFKG 

       190        200        210        220        230        240 
ASYLTRYDLL CQRLVQEQLY TTAAVIAAER SAVDTGNFTE LSSMTSLKTF VSALAGHIAA 


EAARLG 

« Hide

References

[1]"Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products."
Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., Gingeras T.R.
Nucleic Acids Res. 13:8441-8461(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03274 Genomic DNA. Translation: CAA27026.1.
PIRNDPS7A. S07935.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1451. PaeR7I.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007636. Restrct_endonuc_II_XhoI.
[Graphical view]
PfamPF04555. XhoI. 1 hit.
[Graphical view]
PIRSFPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
ProtoNetSearch...

Entry information

Entry nameT2P7_PSEAI
AccessionPrimary (citable) accession number: P05104
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries