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P05104

- T2P7_PSEAI

UniProt

P05104 - T2P7_PSEAI

Protein

Type-2 restriction enzyme PaeR7I

Gene

paeR7IR

Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CTCGAG and cleaves after C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi1451. PaeR7I.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme PaeR7I (EC:3.1.21.4)
    Short name:
    R.PaeR7I
    Alternative name(s):
    Endonuclease PaeR7I
    Type II restriction enzyme PaeR7I
    Gene namesi
    Name:paeR7IR
    Encoded oniPlasmid pMG70 Publication
    OrganismiPseudomonas aeruginosa
    Taxonomic identifieri287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 246245Type-2 restriction enzyme PaeR7IPRO_0000077353Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR007636. Restrct_endonuc_II_XhoI.
    [Graphical view]
    PfamiPF04555. XhoI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05104-1 [UniParc]FASTAAdd to Basket

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    MALDLVDYEQ KARDAVKAFW GNREAARQKQ IESGKADQGE RAGVTGGKNM    50
    DGFLALVLDV IKANGLAHAE IHQNRAMLTL PGYFRPTKLW DLLVIYKGEL 100
    IAAIELKSHV GPSFSNNFNN RTEEAIGTAH DLWTAYREEA FGKQPRPFVG 150
    WLMMVEDAPE SRRPVRDSSP HFPVFEEFKG ASYLTRYDLL CQRLVQEQLY 200
    TTAAVIAAER SAVDTGNFTE LSSMTSLKTF VSALAGHIAA EAARLG 246
    Length:246
    Mass (Da):27,279
    Last modified:January 23, 2007 - v3
    Checksum:i3BC63AC987BDF3BA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03274 Genomic DNA. Translation: CAA27026.1.
    PIRiS07935. NDPS7A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03274 Genomic DNA. Translation: CAA27026.1 .
    PIRi S07935. NDPS7A.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1451. PaeR7I.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR007636. Restrct_endonuc_II_XhoI.
    [Graphical view ]
    Pfami PF04555. XhoI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000994. Restrict_endonuc_II_XhoI. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products."
      Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., Gingeras T.R.
      Nucleic Acids Res. 13:8441-8461(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiT2P7_PSEAI
    AccessioniPrimary (citable) accession number: P05104
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 74 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3